An immunological study of the embryonic stages of the zebra fish, Brachydanio rerio. I. Prehatching-stage antigenic patterns

1975 ◽  
Vol 53 (3) ◽  
pp. 246-252 ◽  
Author(s):  
Hans Laale ◽  
Peter Yin-Hong Law
Keyword(s):  
Developmental Stages ◽  
Immunological Study ◽  
Zebra Fish ◽  
Brachydanio Rerio ◽  
Optic Cup ◽  
Embryonic Shield

Seven developmental stages of the zebra fish, Brachydanio rerio, namely, ovary, cleavage, high blastula, midgastrula, embryonic shield (11–12 h), optic cup, and hatching stages were analyzed by immunodiffusion and immunoelectrophoresis with unabsorbed and absorbed rabbit antisera to five prehatching developmental stages. The precipitin bands are discussed.

An immunological study of the embryonic stages of the zebra fish, Brachydanio rerio. II. Hatching-stage antigenic patterns

1975 ◽  
Vol 53 (3) ◽  
pp. 253-261 ◽  
Author(s):  
Peter Yin-Hong Law ◽  
Hans Laale
Keyword(s):  
Developmental Stage ◽  
Developmental Stages ◽  
Rabbit Antiserum ◽  
Immunological Study ◽  
Zebra Fish ◽  
Brachydanio Rerio

Immunoelectrophoresis and immunodiffusion analyses were performed on the supernates of seven developmental stages of the zebra fish, Brachydanio rerio. The analytical agents were unabsorbed rabbit antiserum against hatching-stage extract, rabbit antisera against all stages (except ovary) absorbed with hatching-stage extract, and rabbit antiserum against hatching-stage extract absorbed respectively with extracts from each developmental stage (except ovary).The results are discussed and compared to the prehatching antigenic patterns reported for Brachydanio rerio.

EFFECTS OF CHLORAMPHENICOL ON THE DEVELOPMENT OF THE ZEBRAFISH, BRACHYDANIO RERIO

1967 ◽  
Vol 45 (2) ◽  
pp. 191-204 ◽  
Author(s):  
P. D. Anderson ◽  
H. I. Battle
Keyword(s):  
Spina Bifida ◽  
Mode Of Action ◽  
Developmental Stages ◽  
Frequent Occurrence ◽  
Brachydanio Rerio ◽  
Unique Type ◽  
Present Concept ◽  
Optic Cup

Fertilized eggs of the zebrafish, Brachydanio rerio, were subjected at 26 °C to three concentrations of chloramphenicol, 0.125, 0.250, and 0.500 mg/ml, at seven developmental stages ranging from cleavage to optic cup formation for periods of 12, 24, and 36 h. Subsequently, they were transferred to aquarial water to complete 48 h of development. Most extreme anomalies were induced by chloramphenicol in eggs initially subjected during cleavage and blastulation and less extreme anomalies in eggs exposed during or after gastrulation. In addition to general retardation of development, anomalies of the nervous, muscular, and vascular systems were induced. A unique type of spina bifida was of frequent occurrence. The teratogenic responses of the zebrafish egg are discussed in relation to the present concept of the mode of action of this antibiotic.

Production of clones of homozygous diploid zebra fish (Brachydanio rerio)

Nature ◽  
1981 ◽  
Vol 291 (5813) ◽  
pp. 293-296 ◽  
Author(s):  
George Streisinger ◽  
Charline Walker ◽  
Nancy Dower ◽  
Donna Knauber ◽  
Fred Singer
Keyword(s):  
Zebra Fish ◽  
Brachydanio Rerio ◽  
Homozygous Diploid

scholarly journals Breeding biology of guppy fish, Poecilia reticulata (Peters, 1859) in the laboratory

2014 ◽  
Vol 39 (2) ◽  
pp. 259-267 ◽  
Author(s):  
Reza Md Shahjahan ◽  
Md Jubayer Ahmed ◽  
Rowshan Ara Begum ◽  
Md Abdur Rashid
Keyword(s):  
Sexual Maturity ◽  
Poecilia Reticulata ◽  
Developmental Stages ◽  
Breeding Biology ◽  
Zoological Garden ◽  
Peak Period ◽  
Optic Cup ◽  
The Mean ◽  
Egg Diameter ◽  
Guppy Fish

The breeding biology of guppy fish, Poecilia reticulata (Cyprinodontiformes: Poiciliidae) was studied during March 2008 to May 2009 in ‘Zoological garden laboratory’, Curzon Hall campus, Dhaka University. Guppy bred all over the year except in the winter months December and January with a peak period in July. They were viviparous and multiple breeders, i.e., give birth to fry several times in the breeding season. The mean egg diameter was measured to be (1.02±0.08mm) and fecundity was estimated (40-89) per gram of body weight. The gestation period ranged 25-35 days with an average of 28.1±2.12 days. Developmental stages observed under a compound microscope were classified based on the changes in the developing eye, such as optic cup, early-eyed, middle-eyed, late-eyed, very late-eyed etc. It was noticed that tail portion comes out first at birth. The number of fry per brood ranged from 12 to 60. New born fries were observed with transparent or blackish in colour having slender body with jaws developed on mouth and were fully capable of swimming, eating, and avoiding danger. Guppy grew rapidly, attained sexual maturity at 8 -10 weeks and reached full size in 6 months. DOI: http://dx.doi.org/10.3329/jasbs.v39i2.17866 J. Asiat. Soc. Bangladesh, Sci. 39(2): 259-267, December 2013

Zebra fish myc family and max genes: differential expression and oncogenic activity throughout vertebrate evolution

1993 ◽  
Vol 13 (5) ◽  
pp. 2765-2775
Author(s):  
N Schreiber-Agus ◽  
J Horner ◽  
R Torres ◽  
F C Chiu ◽  
R A DePinho
Keyword(s):  
Steady State ◽  
Mammalian Cells ◽  
Cellular Growth ◽  
Zebra Fish ◽  
Brachydanio Rerio ◽  
Organ Systems ◽  
Myc Gene ◽  
Transformation Activity ◽  
Mammalian Counterpart ◽  
High Degree

To gain insight into the role of Myc family oncoproteins and their associated protein Max in vertebrate growth and development, we sought to identify homologs in the zebra fish (Brachydanio rerio). A combination of a polymerase chain reaction-based cloning strategy and low-stringency hybridization screening allowed for the isolation of zebra fish c-, N-, and L-myc and max genes; subsequent structural characterization showed a high degree of conservation in regions that encode motifs of known functional significance. On the functional level, zebra fish Max, like its mammalian counterpart, served to suppress the transformation activity of mouse c-Myc in rat embryo fibroblasts. In addition, the zebra fish c-myc gene proved capable of cooperating with an activated H-ras to effect the malignant transformation of mammalian cells, albeit with diminished potency compared with mouse c-myc. With respect to their roles in normal developing tissues, the differential temporal and spatial patterns of steady-state mRNA expression observed for each zebra fish myc family member suggest unique functions for L-myc in early embryogenesis, for N-myc in establishment and growth of early organ systems, and for c-myc in increasingly differentiated tissues. Furthermore, significant alterations in the steady-state expression of zebra fish myc family genes concomitant with relatively constant max expression support the emerging model of regulation of Myc function in cellular growth and differentiation.

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