Protoplasmic fragments in haematopoietic tissues and analysis of intranuclear vesicles in lymph node blast cells of the rabbit

1970 ◽  
Vol 48 (4) ◽  
pp. 709-716 ◽  
Author(s):  
Jean E. Mills Westermann ◽  
Jessica L. G. Shelley ◽  
Vibeke E. Engelbert
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Cell Formation ◽  
Lymphoid Cells ◽  
Mesenteric Lymph Nodes ◽  
Mesenteric Lymph ◽  
Blood Smears ◽  
Blast Cells

Vesicles and cytoplasmic fragments are found in greatest numbers in the lymph node and spleen and least commonly in the thymus and bone narrow in the rabbit. Vesicles appear to originate by the extrusion of intranuclear and intracytoplasmic vesicles mostly from cells of the lymphoid series. Cytoplasmic fragments formed by the pinching off of cytoplasmic buds of blast and lymphoid cells may be either round, oval, or irregular in shape. Vesicles and cytoplasmic fragments are absent from blood smears and extremely difficult to recognize in sections or in areas of imprints where the cells are closely applied one to another. About one-third of all round to oval "naked" blast cells in imprints of mesenteric lymph nodes contain vesicles ranging to 7 μ in diameter although most are about 1 μ in diameter. Large "naked" nuclei contain more vesicles than expected although the size of the nucleus does not affect the size of the vesicles present. We suggest that chromatin from "naked" nuclei and smaller free chromatin masses may become transferred to free vesicles and this process may function in new cell formation.

scholarly journals Immunocytochemistry of mesenteric lymph node fine-needle aspirates in the diagnosis of feline infectious peritonitis

2019 ◽  
Vol 31 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Sandra Felten ◽  
Katrin Hartmann ◽  
Stefanie Doerfelt ◽  
Laura Sangl ◽  
Johannes Hirschberger ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Complex Method ◽  
Mesenteric Lymph Nodes ◽  
Predictive Values ◽  
Feline Infectious Peritonitis ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Mesenteric Lymph

Immunohistochemistry (IHC) of tissue samples is considered the gold standard for diagnosing feline infectious peritonitis (FIP), and, in cats without body cavity effusion, IHC is the only method available to establish definitive antemortem diagnosis. However, IHC requires invasive tissue sample collection. We evaluated sensitivity and specificity of an immunocytochemical assay of fine-needle aspirates (FNAs) of mesenteric lymph nodes that can be obtained noninvasively by ultrasound-guided aspiration to diagnose FIP. FNAs of mesenteric lymph nodes were obtained postmortem from 41 cats suspected of having FIP based on clinical and/or laboratory findings. FIP was confirmed immunohistochemically in 30 cats. In the other 11 cats, a disease other than FIP, which explained the clinical signs, was diagnosed histopathologically. Immunocytochemistry (ICC) was performed as an avidin–biotin complex method using a monoclonal anti-FCoV IgG 2A. Sensitivity, specificity, negative and positive predictive values (NPV, PPV, respectively) including 95% confidence intervals (95% CIs) were determined. ICC was positive in 17 of 30 cats with FIP, but also in 1 of 11 control cats that was diagnosed with lymphoma. Sensitivity of ICC was 53% (95% CI: 34–72); specificity 91% (95% CI: 59–100); NPV 42% (95% CI: 22–63); and PPV 94% (95% CI: 71–100). In a lethal disease such as FIP, specificity is most important in order to avoid euthanasia of unaffected cats. Given that a false-positive result occurred and FIP was correctly detected in only approximately half of the cases of FIP, ICC of mesenteric lymph node FNA alone cannot reliably confirm or exclude FIP, but can be a helpful test in conjunction with other diagnostic measures.

scholarly journals THE ROLE OF THE LYMPHATIC SYSTEM IN THE REJECTION OF HOMOGRAFTS: A STUDY OF LYMPH FROM RENAL TRANSPLANTS

1970 ◽  
Vol 131 (5) ◽  
pp. 936-969 ◽  
Author(s):  
Niels C. Pedersen ◽  
Bede Morris
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
The Body ◽  
Lymphoid Cells ◽  
Host Cells ◽  
Kidney Graft ◽  
Blood Capillaries ◽  
Renal Lymph ◽  
Peritubular Capillaries ◽  
Blast Cells

The rejection of renal homografts has been studied in sheep by transplanting kidneys into the neck and preserving the renal lymphatic drainage intact. Chronic fistulae were established in the transplanted renal lymphatics and lymph collected throughout the life of the graft. The changes that occurred in homografts during the process of rejection were reflected in changes in the lymph. Large numbers of basophilic, blast, lymphoid cells appeared in the lymph, and lymph production in the grafted kidney increased 20–50 fold. Over a period of about 10 days, up to 60 g wet weight of lymphoid cells and up to 10 liters of lymph were collected from the graft. Within 24 hr of grafting, the host cells present in the renal lymph had become sensitized to the graft and transformed into blast cells when cultivated in Millipore chambers in vitro. When the cells leaving the graft during the first 18–48 hr were injected into distant nonstimulated lymph nodes of the host sheep, they evoked significant cellular and antibody responses in the nodes. Within the graft, the main pathological changes were found in the vascular endothelium and many of the peritubular capillaries become plugged with emboli comprised of blast cells. There was extensive infiltration of the renal parenchyma with lymphoid cells and evidence of their transformation and proliferation within the renal blood capillaries. When all the lymph and cells leaving the homograft were diverted from the body, there was a greatly decreased reaction in the regional prescapular lymph node, and no reaction in lymph nodes distant from the graft. In these circumstances, the survival of the graft was not prolonged, and it was rejected without involvement of the lymph nodes of the host. Humoral antibody was produced in the lymph node regional to the homograft within 48–60 hr of grafting. Antibody was not detected in the blood or in the renal lymph until near to the time the graft was rejected. It was thought that this was due to the binding of antibody by the kidney graft tissue. We conclude that all the events which lead to the recognition and rejection of renal homografts can occur centrally within the graft itself.

The structure of the thymus and mesenteric lymph nodes of newborn rats as a result of the prenatal influence of ethanol

2015 ◽  
Vol 6 (4) ◽  
pp. 51-55 ◽  
Author(s):  
Petr Vladimirovich Pugach ◽  
Sergey Vladimirovich Kruglov ◽  
Natalia Rafailovna Karelina ◽  
Dmitriy Vitalievich Breusenko ◽  
Stepan Yurevich Bazhin ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Morphological Changes ◽  
Alcohol Intoxication ◽  
Sole Source ◽  
Structural Features ◽  
Lymphoid Cells ◽  
Newborn Rats ◽  
Mesenteric Lymph Nodes ◽  
White Rats ◽  
Mesenteric Lymph

The present study was undertaken to examine the structural features of the thymus and cranial mesenteric lymph nodes of newborn rats that have occurred as a result of antenatal alcohol intoxication. We used a set of morphometric, anatomical and histological methods. A study of the cranial mesenteric lymph nodes performed on 45 newborn rats born to 18 female mongrel white rats seven months of age. The studies were conducted in compliance with the order of the Ministry of Health of the USSR N 755 from 12.08.1977 and the order of the Ministry of Higher and Secondary Special Education of the USSR from 13.11.1984, "On the rules of work with experimental animals". Females, on which was received investigated offspring exposed to 15 % ethanol as the sole source of fluid for 1 week, one and three months before pregnancy, during pregnancy and after its completion. It is shown that depending on the duration of pregravid exposure to ethanol occur progredient changes in the structure of the thymus and cranial mesenteric lymph nodes. In the thymus, as well as in the lymph nodes, decreases the number of lymphoid cells and stromal elements content increases. In lymph nodes there are significant changes in the structure of the sinus system. The severity of the identified morphological changes due to the duration of the effects of alcohol on the system "mother-placenta-fetus”.

scholarly journals Fulminant Liver Failure with Necrotizing Foci in the Liver, Spleen and Lymph Nodes in Celiac Disease Due to Malignant Lymphoma

1996 ◽  
Vol 10 (4) ◽  
pp. 225-229 ◽  
Author(s):  
Hugh James Freeman
Keyword(s):  
Celiac Disease ◽  
T Cell ◽  
Lymph Nodes ◽  
Liver Failure ◽  
Cell Receptor ◽  
Lymphoid Cells ◽  
Mesenteric Lymph Nodes ◽  
Rare Type ◽  
Mesenteric Lymph ◽  
Spleen And Lymph Nodes

A 65-year-old female with celiac disease developed cholestatic jaundice and fatal liver failure. Investigations revealed widespread necrotic foci in the liver, spleen and mesenteric lymph nodes, changes reminiscent of the mesenteric lymph node cavitation syndrome, which is known to complicate celiac disease. In addition, malignant lymphoid cells were present infiltrating hepatic sinusoids, lymph nodes and spleen. These features are typical of hepatosplenic lymphoma, a rare type of peripheral T cell lymphoma with T cell receptor rearrangement. Lymphorecticular malignancy complicating celiac disease may present with fulminant liver disease.

scholarly journals Metastasis to lateral lymph nodes with no mesenteric lymph node involvement in low rectal cancer: a retrospective case series

2020 ◽  
Author(s):  
Peng Li ◽  
Zhichun Zhang ◽  
Yuanda Zhou ◽  
Qingsheng Zeng ◽  
Xipeng Zhang ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Dissection ◽  
Low Rectal Cancer ◽  
Lateral Lymph Node Dissection ◽  
Node Dissection ◽  
Mesenteric Lymph Nodes ◽  
Lateral Lymph Node ◽  
Mesenteric Lymph

Abstract Purpose The aim of this study is to examine the pattern of lymph node metastasis (lateral vs. mesenteric lymph nodes) in low rectal cancer.Methods This retrospective analysis included all patients undergoing laparoscopic total mesorectal excision plus lateral lymph node dissection for advanced low rectal cancer (up to 8 cm from the anal verge) during a period from July 1, 2017 to August 31, 2019 at the Department of Colorectal Surgery, Tianjin Union Medical Center. The decision to conduct lateral lymph node dissection was based on positive findings in preoperative imaging assessments.Results A total of 42 patients were included in data analysis. Surgery was successfully completed as planned, without conversion to open surgery in any case. A minimal of 10 mesenteric lymph nodes and 1 lateral lymph node on each side were dissected in all patients. Pathologic examination of resected specimens showed no metastasis to either mesenteric or lateral lymph nodes in 7 (16.7%) case, metastasis to both mesenteric and lateral lymph nodes in 26 (61.9%) cases, metastasis to mesenteric but not lateral lymph nodes in 4 (9.5%) cases, and metastasis to lateral but not mesenteric lymph nodes in 5 (11.9%) cases (n=2 in the obturator region; n=3 in the iliac artery region).Conclusion A clinically significant proportion of low rectal cancer patients have metastasis to lateral lymph nodes without involvement of mesenteric lymph nodes. More carefully planned prospective studies are needed to verify this preliminary finding.

scholarly journals Metastasis to lateral lymph nodes with no mesenteric lymph node involvement in low rectal cancer: a retrospective case series

2020 ◽  
Author(s):  
Peng Li ◽  
Zhichun Zhang ◽  
Yuanda Zhou ◽  
Qingsheng Zeng ◽  
Xipeng Zhang ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Dissection ◽  
Low Rectal Cancer ◽  
Lateral Lymph Node Dissection ◽  
Node Dissection ◽  
Mesenteric Lymph Nodes ◽  
Lateral Lymph Node ◽  
Mesenteric Lymph

Abstract Purpose The aim of this study is to examine the pattern of lymph node metastasis (lateral vs. mesenteric lymph nodes) in low rectal cancer.Methods This retrospective analysis included all patients undergoing laparoscopic total mesorectal excision plus lateral lymph node dissection for advanced low rectal cancer (up to 8 cm from the anal verge) during a period from July 1, 2017 to August 31, 2019 at the Department of Colorectal Surgery, Tianjin Union Medical Center. The decision to conduct lateral lymph node dissection was based on positive findings in preoperative imaging assessments.Results A total of 42 patients were included in data analysis. Surgery was successfully completed as planned, without conversion to open surgery in any case. A minimum of 10 mesenteric lymph nodes and 1 lateral lymph node on each side were dissected in all patients. Pathologic examination of resected specimens showed no metastasis to either mesenteric or lateral lymph nodes in 7 (16.7%) case, metastasis to both mesenteric and lateral lymph nodes in 26 (61.9%) cases, metastasis to mesenteric but not lateral lymph nodes in 4 (9.5%) cases, and metastasis to lateral but not mesenteric lymph nodes in 5 (11.9%) cases (n=2 in the obturator region; n=3 in the iliac artery region).Conclusion A clinically significant proportion of low rectal cancer patients have metastasis to lateral lymph nodes without involvement of mesenteric lymph nodes. More carefully planned prospective studies are needed to verify this preliminary finding.

scholarly journals CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Emma C. Mackley ◽  
Stephanie Houston ◽  
Clare L. Marriott ◽  
Emily E. Halford ◽  
Beth Lucas ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Lymphoid Cells ◽  
Intestinal Cells ◽  
Lymphoid Tissues ◽  
Mesenteric Lymph Nodes ◽  
Cell Responses ◽  
Mesenteric Lymph ◽  
Peripheral Lymph ◽  
Group 3 ◽  
Draining Lymph Nodes

Abstract Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

scholarly journals MAGNITUDE AND PATTERN OF THYMIC LYMPHOCYTE MIGRATION IN NEONATAL MICE

1972 ◽  
Vol 135 (4) ◽  
pp. 907-923 ◽  
Author(s):  
D. D. Joel ◽  
M. W. Hess ◽  
H. Cottier
Keyword(s):  
Bone Marrow ◽  
Cell Migration ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Peyer's Patches ◽  
Mesenteric Lymph Nodes ◽  
Neonatal Mice ◽  
Mesenteric Lymph ◽  
The Mean ◽  
Thymic Cell

Neonatal mice were given a subcapsular, intrathymic injection of thymidine-3H using a modified microneedle technique, and the migration of labeled cells to spleen, lymph nodes, Peyer's patches, and bone marrow was followed radioautographically with time. Assuming that nonlabeled lymphocytes migrated in the same manner as labeled lymphocytes, it can be concluded that the majority of lymphocytes present within mesenteric lymph nodes (74%) and Peyer's patches (61%), and a large proportion of those located in popliteal lymph nodes (40%) and the spleen (26%), were of thymic origin. Evidence is presented indicating that these are minimum values. The difference in the magnitude of thymic cell migration to gut-associated lymphoid tissue on the one hand and to the spleen and popliteal lymph node on the other hand was tentatively attributed to antigenic stimulation from the intestinal flora which develops during the first days of life. Thymus-derived lymphocytes were scattered throughout the lymph node cortex and splenic follicles. No noticeable thymic cell migration to the bone marrow was found. Labeling indices in the peripheral lymphoid organs paralleled those of cortical thymic lymphocytes suggesting the thymic cortex as the major source of migrants. By 2 days postinjection, the mean grain counts of labeled lymphocytes in all peripheral lymphoid tissues were higher than the mean grain counts of labeled lymphocytes in the thymus. At 7 days postinjection heavily labeled cells constituted 11–16% of the labeled population in peripheral tissues while they were absent from the thymic cortex. These results indicate that a fraction of thymus-derived cells, upon settling in the periphery, remained in, or reentered, a nonproliferative phase for at least 7 days. Conversely, many thymus-derived lymphocytes underwent division in the periphery and/or penetrated the intestinal epithelium. Since the relative number of thymus-derived cells found in the mesenteric lymph nodes of 1- and 2-day old mice was considerably higher than the percentage of cells at this site having the theta (θ) alloantigen, as reported by other authors, the possibility exists that θ-antigen on thymus-derived lymphocytes may, at least in a fraction of these cells, no longer be detectable as they reach the peripheral organs.

scholarly journals Metastasis to lateral lymph nodes with no mesenteric lymph node involvement in low rectal cancer: a retrospective case series

2020 ◽  
Author(s):  
Peng Li ◽  
Zhichun Zhang ◽  
Yuanda Zhou ◽  
Qingsheng Zeng ◽  
Xipeng Zhang ◽  
...  
Keyword(s):  
Rectal Cancer ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Lymph Node Dissection ◽  
Low Rectal Cancer ◽  
Lateral Lymph Node Dissection ◽  
Node Dissection ◽  
Mesenteric Lymph Nodes ◽  
Lateral Lymph Node ◽  
Mesenteric Lymph

Abstract Purpose The aim of this study is to examine the pattern of lymph node metastasis (lateral vs. mesenteric lymph nodes) in low rectal cancer.Methods This retrospective analysis included all patients undergoing laparoscopic total mesorectal excision plus lateral lymph node dissection for advanced low rectal cancer (up to 8 cm from the anal verge) during a period from July 1, 2017 to August 31, 2019 at the Department of Colorectal Surgery, Tianjin Union Medical Center. The decision to conduct lateral lymph node dissection was based on positive findings in preoperative imaging assessments.Results A total of 42 patients were included in data analysis. Surgery was successfully completed as planned, without conversion to open surgery in any case. A minimumal of 10 mesenteric lymph nodes and 1 lateral lymph node on each side were dissected in all patients. Pathologic examination of resected specimens showed no metastasis to either mesenteric or lateral lymph nodes in 7 (16.7%) case, metastasis to both mesenteric and lateral lymph nodes in 26 (61.9%) cases, metastasis to mesenteric but not lateral lymph nodes in 4 (9.5%) cases, and metastasis to lateral but not mesenteric lymph nodes in 5 (11.9%) cases (n=2 in the obturator region; n=3 in the iliac artery region).Conclusion A clinically significant proportion of low rectal cancer patients have metastasis to lateral lymph nodes without involvement of mesenteric lymph nodes. More carefully planned prospective studies are needed to verify this preliminary finding.

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