The fine structure of the cuticle and interchordal hypodermis of the parasitic nematodes, Capillaria hepatica and Trichuris myocastoris

1968 ◽  
Vol 46 (2) ◽  
pp. 173-179 ◽  
Author(s):  
K. A. Wright
Keyword(s):  
Light And Electron Microscopy ◽  
Basal Layer ◽  
Outer Region ◽  
Parasitic Nematodes ◽  
Cortical Layers ◽  
Capillaria Hepatica ◽  
Permeability Characteristics ◽  
Matrix Layer ◽  
The Matrix ◽  
Cell Processes

The cuticle and underlying hypodermis of the parasitic nematodes, Capillaria hepatica and Trichuris myocastoris, were examined by light and electron microscopy. The cuticle in both species consists of a filamentous, collagenous, basal layer, which contains a zone of striated material in its outer region; a matrix layer; and thin cortical layers which may appear as three osmiophilic lines. It is suggested that the striated layer of the cuticle provides the tensile strength given by fiber layers in the cuticle of other species. The matrix layer of T. myocastoris contains large amounts of non-glycogen polysaccharide while the matrix layer of C. hepatica does not. It is speculated that the polysaccharide content of the cuticle may determine its permeability characteristics. The interchordal hypodermis contains little endoplasmic reticulum, very few Golgi zones, but abundant mitochondria. In C. hepatica the interchordal hypodermis is formed of cell processes from cells of the lateral and median hypodermal chords, while in T. myocastoris, it is cellular.

scholarly journals Damage and repair of the peripheral myelin sheath and node of Ranvier after treatment with trypsin.

1975 ◽  
Vol 64 (1) ◽  
pp. 1-14 ◽  
Author(s):  
R C Yu ◽  
R P Bunge
Keyword(s):  
Schwann Cell ◽  
Light And Electron Microscopy ◽  
Node Of Ranvier ◽  
Nerve Fibers ◽  
Myelinated Nerve ◽  
Nodal Region ◽  
Fetal Rat ◽  
The Matrix ◽  
Cell Processes

Cultures of whole fetal rat sensory ganglia which had matured and myelinated in culture were treated for 1-3 h with a pulse of 0.2% trypsin. The tissue was observed during the period of treatment and during subsequent weeks using both light and electron microscopy. Within minutes after trypsin addition the matrix of the culture was altered and the nerve fascicles loosened. Progressive changes included the retraction of Schwann cell processes from the nodal region the detachment of the myelin-related paranodal Schwann cell loops from the axon, and lengthening of the nodal region as the axon was bared. The retraction of myelin from nodal stabilized several hours after trypsin withdrawal. Breakdown of the altered myelin segments was rare. There were no discernable changes in neurons or their processes after this exposure to trypsin. The partial repair which occured over a period of several weeks included the reattachment of paranodal Schwann cell loops to the axolemma and the insertion of new myelin segments where a substantial length of axolemma had been bared. The significance of these observations to the characterization of the Schwann cell-axolemmal junctions on myelinated nerve fibers is discussed. The dramatic degree of myelin change that can occur without concomitant myelin breakdown is particularly noted, as is the observation that these altered myelin segments are, in part, repaired.

scholarly journals Tenascin/hexabrachion in human skin: biochemical identification and localization by light and electron microscopy.

1989 ◽  
Vol 108 (6) ◽  
pp. 2483-2493 ◽  
Author(s):  
V A Lightner ◽  
F Gumkowski ◽  
D D Bigner ◽  
H P Erickson
Keyword(s):  
Human Skin ◽  
Basal Lamina ◽  
Guanidine Hydrochloride ◽  
Light And Electron Microscopy ◽  
Basal Layer ◽  
Papillary Dermis ◽  
Sweat Glands ◽  
Elastic Fibers ◽  
Normal Human Skin ◽  
The Matrix

Tenascin/hexabrachion is a large glycoprotein of the extracellular matrix. Previous reports have demonstrated that tenascin is associated with epithelial-mesenchymal interfaces during embryogenesis and is prominent in the matrix of many tumors. However, the distribution of tenascin is more restricted in adult tissues. We have found tenascin to be present in normal human skin in a distribution distinct from other matrix proteins. Immunohistochemical studies showed staining of the papillary dermis immediately beneath the basal lamina. Examination of skin that had been split within the lamina lucida of the basement membrane suggested a localization of tenascin beneath the lamina lucida. In addition, there was finely localized staining within the walls of blood vessels and in the smooth muscle bundles of the arrectori pilorem. Very prominent staining was seen around the cuboidal cells that formed the basal layer of sweat gland ducts. The sweat glands themselves did not stain. The distribution of tenascin in the papillary dermis was studied at high resolution by immunoelectron microscopy. Staining was concentrated in small amorphous patches scattered amongst the collagen fibers beneath the basal lamina. These patches were not associated with cell structures, collagen, or elastic fibers. Tenascin could be partially extracted from the papillary dermis by urea, guanidine hydrochloride, or high pH solution. The extracted protein showed a 320-kD subunit similar to that purified from fibroblast or glioma cell cultures. We have developed a sensitive ELISA assay that can quantitate tenascin at concentrations as low as 5 ng/ml. Tests on extracts of the papillary dermis showed tenascin constituted about 0.02-0.05% of the protein extracted.

Organisation in the Structure of the Cytoplasmic Ground Substance

1978 ◽  
Vol 36 (3) ◽  
pp. 627-639
Author(s):  
K.R. Porter
Keyword(s):  
Endoplasmic Reticulum ◽  
Golgi Complex ◽  
Random Distribution ◽  
Ground Substance ◽  
The Matrix ◽  
Cell Processes ◽  
Cytoplasmic Ground Substance

Most types of cells are known from their structure and overall form to possess a characteristic organization. In some instances this is evident in the non-random disposition of organelles and such system subunits as cisternae of the endoplasmic reticulum or the Golgi complex. In others it appears in the distribution and orientation of cytoplasmic fibrils. And in yet others the organization finds expression in the non-random distribution and orientation of microtubules, especially as found in highly anisometric cells and cell processes. The impression is unavoidable that in none of these cases is the organization achieved without the involvement of the cytoplasmic ground substance (CGS) or matrix. This impression is based on the fact that a matrix is present and that in all instances these formed structures, whether membranelimited or filamentous, are suspended in it. In some well-known instances, as in arrays of microtubules which make up axonemes and axostyles, the matrix resolves itself into bridges (and spokes) between the microtubules, bridges which are in some cases very regularly disposed and uniform in size (Mcintosh, 1973; Bloodgood and Miller, 1974; Warner and Satir, 1974).

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

scholarly journals A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography

BMC Biology ◽  
2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sergio Gabarre ◽  
Frank Vernaillen ◽  
Pieter Baatsen ◽  
Katlijn Vints ◽  
Christopher Cawthorne ◽  
...  
Keyword(s):  
High Resolution ◽  
Light And Electron Microscopy ◽  
User Interaction ◽  
Regions Of Interest ◽  
Imaging Method ◽  
Array Tomography ◽  
Reference Space ◽  
Cell Processes ◽  
Electron Microscopes ◽  
Specific Tissue

Abstract Background Array tomography (AT) is a high-resolution imaging method to resolve fine details at the organelle level and has the advantage that it can provide 3D volumes to show the tissue context. AT can be carried out in a correlative way, combing light and electron microscopy (LM, EM) techniques. However, the correlation between modalities can be a challenge and delineating specific regions of interest in consecutive sections can be time-consuming. Integrated light and electron microscopes (iLEMs) offer the possibility to provide well-correlated images and may pose an ideal solution for correlative AT. Here, we report a workflow to automate navigation between regions of interest. Results We use a targeted approach that allows imaging specific tissue features, like organelles, cell processes, and nuclei at different scales to enable fast, directly correlated in situ AT using an integrated light and electron microscope (iLEM-AT). Our workflow is based on the detection of section boundaries on an initial transmitted light acquisition that serves as a reference space to compensate for changes in shape between sections, and we apply a stepwise refinement of localizations as the magnification increases from LM to EM. With minimal user interaction, this enables autonomous and speedy acquisition of regions containing cells and cellular organelles of interest correlated across different magnifications for LM and EM modalities, providing a more efficient way to obtain 3D images. We provide a proof of concept of our approach and the developed software tools using both Golgi neuronal impregnation staining and fluorescently labeled protein condensates in cells. Conclusions Our method facilitates tracing and reconstructing cellular structures over multiple sections, is targeted at high resolution ILEMs, and can be integrated into existing devices, both commercial and custom-built systems.

The Relationship Between Magnetism and Microstructure of Ethylene Pyrolysis Furnace Tubes after a Long-term Service

2018 ◽  
Vol 24 (5) ◽  
pp. 478-487 ◽  
Author(s):  
Jingfeng Guo ◽  
Tieshan Cao ◽  
Congqian Cheng ◽  
Xianming Meng ◽  
Jie Zhao
Keyword(s):  
Saturation Magnetization ◽  
Outer Region ◽  
Cr Content ◽  
Pyrolysis Furnace ◽  
The Matrix ◽  
Furnace Tube ◽  
Primary Carbides ◽  
The Relationship ◽  
Very High

AbstractThe magnetism and microstructure of Cr25Ni35Nb and Cr35Ni45Nb alloy tubes after 5 years of service were investigated in this paper. The saturation magnetization of the Cr25Ni35Nb alloy tube in the thickness direction is more than 20 emu/g, and the tube becomes ferromagnetic. The inner and outer walls of Cr35Ni45Nb alloy tubes also become ferromagnetic. But the saturation magnetization of the Cr35Ni45Nb alloy tubes approaches to zero in the center zone. The primary carbides M7C3 and NbC are changed into M23C6 and G phase at the outer region of the furnace tube. However, the M23C6-type carbides were replaced by carbon-rich carbides M7C3 at the carburization zone. Cr-depleted zones are formed at the inner and outer walls of the furnace tubes owing to oxidation. Carburization and oxidation reduce the Cr content of the matrix. Accordingly, the saturation magnetization is very high at the carburization zone and Cr-depleted zone. The magnetism of Cr25Ni35Nb and Cr35Ni45Nb alloy tubes has a high correlation with the Cr content of the matrix. Carburization and oxidation are the main reasons that make the paramagnetic ethylene pyrolysis furnace tube change to ferromagnetic.

A developmental and ultrastructural study of the optic chiasma in Xenopus

Development ◽  
1988 ◽  
Vol 102 (3) ◽  
pp. 537-553
Author(s):  
M.A. Wilson ◽  
J.S. Taylor ◽  
R.M. Gaze
Keyword(s):  
Optic Nerve ◽  
Cell Mass ◽  
Light And Electron Microscopy ◽  
Optic Tract ◽  
Retinal Ganglion ◽  
Intercellular Spaces ◽  
Optic Chiasma ◽  
Cell Processes ◽  
Optic Axons ◽  
The Brain

The structure of the optic chiasma in Xenopus tadpoles has been investigated by light and electron microscopy. Where the optic nerve approaches the chiasma, a tongue of cells protrudes from the periventricular cell mass into the dorsal part of the nerve. Glial processes from this tongue of cells ensheath fascicles of optic axons as they enter the brain. Coincident with this partitioning, the annular arrangement of axons in the optic nerve changes to the laminar organization of the optic tract. Beyond the site of this rearrangement, all newly growing axons accumulate in the ventral-most part of the nerve and pass into the region between the periventricular cells and pia which we have called the ‘bridge’. This region is characterized by a loose meshwork of glial cell processes, intercellular spaces and the presence of both optic and nonoptic axons. In the bridge, putative growth cones of retinal ganglion cell axons are found in the intercellular spaces in contact with both the glia and with other axons. The newly growing axons from each eye cross in the bridge at the midline and pass into the superficial layers of the contralateral optic tracts. As the system continues to grow, previous generations of axon, which initially crossed in the existing bridge, are displaced dorsally and caudally, forming the deeper layers of the chiasma. At their point of crossing in the deeper layers, these fascicles of axons from each eye interweave in an intimate fashion. There is no glial segregation of the older axons as they interweave within the chiasma.

Laboratory Study of Corrosion of an Alumina Refractory by Molten Potassium Salts

2010 ◽  
Vol 70 ◽  
pp. 65-71 ◽  
Author(s):  
Na Li ◽  
Leena Hupa ◽  
Patrik Yrjas ◽  
Mikko Hupa
Keyword(s):  
Cross Sections ◽  
Molar Ratio ◽  
Biomass Combustion ◽  
Silicate Matrix ◽  
Potassium Salts ◽  
Scale Method ◽  
Matrix Layer ◽  
The Matrix ◽  
Alumina Refractory ◽  
Refractory Surface

The increasing use of biomass and waste derived fuels in combustion challenges the chemical durability of refractories. Durability of an alumina refractory was studied in a chemically aggressive environment. A mixture of potassium chloride and carbonate (molar ratio 1:9) was placed on the sample and heated at 700-1000°C in an electric laboratory furnace in air for one week. Cross-sections of the samples were studied by SEM-EDXA to determine penetration of potassium in the refractory. Potassium was found only in the silicate matrix phase of the alumina refractory. Penetration of potassium decreased steeply from the surface to 1 mm, after which the decrease was linear but varied with temperature. At 700 and 800°C the thickness of the matrix layer that had reacted with potassium was 3 mm, while the layer was thinner at 900 and 1000°C. At the higher temperatures a glassy layer consisting of K2O, Na2O, CaO and SiO2 formed on the refractory surface. At 900°C the thickness of the surface layer was of 10μm, while a 200μm layer was measured at 1000°C. The procedure used in this work can be used to develop a laboratory scale method to be used to study corrosion of refractories in biomass combustion devices.

Effect of subcellular matrix on glycosaminoglycan synthesis by human lung fibroblasts

1987 ◽  
Vol 63 (6) ◽  
pp. 2181-2188 ◽  
Author(s):  
D. J. Cui ◽  
B. A. Dubaybo ◽  
R. A. Durr ◽  
L. A. Thet
Keyword(s):  
Type I Collagen ◽  
Human Lung ◽  
Basement Membranes ◽  
Lung Fibroblasts ◽  
Type I ◽  
Glycosaminoglycan Synthesis ◽  
Human Lung Fibroblasts ◽  
Cell Matrix ◽  
Matrix Layer ◽  
The Matrix

The influences modulating glycosaminoglycan production by lung cells are not well understood. We examined the effect of three different subcellular matrices, plastic, type I collagen, and reconstituted basement membrane-like material (RBM), on the synthesis of sulfated glycosaminoglycans by cultured IMR-90 human lung fibroblasts. Accumulation of 35SO4-labeled glycosaminoglycans into the cell-matrix layer or medium was measured. Cells on collagen synthesized significantly less total glycosaminoglycans than cells on plastic but had a higher fraction of labeled glycosaminoglycans present in the cell-matrix layer (35 vs. 18%) with the increases being highest for dermatan and chondroitin sulfates. Cells grown on the RBM synthesized significantly more glycosaminoglycans than cells on plastic or collagen and also had 260% more labeled glycosaminoglycans present in the cell-matrix layer than cells on plastic. We conclude that the matrix to which lung fibroblasts are exposed can influence the amount and type of glycosaminoglycans synthesized and the degree of incorporation into the matrix. This may be relevant to fibrotic lungs with increased type I collagen or to severely injured lungs in which intra-alveolar fibroblasts are in contact with denuded basement membranes.

Multi-Scale Analysis of a Refractory Composite

2005 ◽  
Author(s):  
Y. W. Kwon ◽  
T. Chu ◽  
D. Kim
Keyword(s):  
Coating Layer ◽  
Matrix Material ◽  
Design Tool ◽  
Scale Model ◽  
High Temperature Application ◽  
Multi Scale ◽  
Matrix Layer ◽  
The Matrix ◽  
Temperature Application ◽  
Refractory Composite

A refractory composite for a high temperature application was studied at various length scales, and its effective thermo-mechanical properties were computed. The analysis considered a micro-scale model made of a representative carbon fiber, a matrix layer, and a coating layer. The model included weak tangential bonding of the intra-layer of the matrix material in order to reduce the thermal stress occurring in the coating material caused by mismatch of coefficients of thermal expansions. In addition, unit-cell models for a 3-D braided composite and a plane-weave composite were also studied. The modeling technique developed in this study can be used as a design tool for an optimal refractory composite for a given application.

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