EFFECTS OF DIETETIC NUCLEIC ACIDS AND COMPONENTS ON GROWTH OF AGRIA AFFINIS (FALLÉN) (DIPTERA: SARCOPHAGIDAE)

1964 ◽  
Vol 42 (5) ◽  
pp. 801-806 ◽  
Author(s):  
H. L. House
Keyword(s):  
Nucleic Acids ◽  
Growth Rates ◽  
Deoxyribonucleic Acid ◽  
Optimal Growth ◽  
Insect Larvae ◽  
Ecological Adaptations ◽  
Adenylic Acid ◽  
Pyrimidine Bases ◽  
Derivatives Of ◽  
Respective Species

Feeding tests on chemically defined diets; showed that dietary nucleic acids are needed for optimal growth rates of the parasitoid Agria affinis (Fallén) reared axenically. Both ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) met this requirement qualitatively and were almost equal quantitatively. The nucleotides adenylic acid, guanylic acid, cytidylic acid, and uridylic acid were the only derivatives of RNA used by the insect larvae to promote growth rates. These nucleotides were equally well utilized regardless of whether their base was purine or pyrimidine. No evidence was found for the utilization of single nucleosides or purine and pyrimidine bases. Phosphorylation of the molecule seemed to be the primary need that decided the utilization of dietary nucleic acid components in this species of Diptera. This augments evidence that various species of Diptera utilize nucleic acids and their derivatives differently. Moreover, it supports an attractive hypothesis of other workers that differences in utilization derive from ecological adaptations of the respective species and more greatly concern the needs for non-essential nutrients than for the essential ones.

INCORPORATION OF RADIOACTIVE COMPOUNDS INTO THE PURINE AND PYRIMIDINE BASES AND 2-DEOXYRIBOSE OF SALMON TESTES DEOXYRIBONUCLEIC ACID

1966 ◽  
Vol 44 (11) ◽  
pp. 1435-1446 ◽  
Author(s):  
H. L. A. Tars ◽  
Joan Roy
Keyword(s):  
Deoxyribonucleic Acid ◽  
De Novo ◽  
Biosynthetic Pathways ◽  
De Novo Biosynthesis ◽  
Adenylic Acid ◽  
Pyrimidine Bases ◽  
Purines And Pyrimidines

Slices of immature salmon testes incorporated radioactive purine and pyrimidine bases and related nucleosides, deoxynucleosides, nucleotides, and deoxynucleotides into deoxyribonucleic acid (DNA). When DNA was extracted from the tissues and hydrolyzed, the purine and pyrimidine bases obtained were labeled in a manner which suggested that the radioactive substrates had been incorporated into DNA by recognized biosynthetic pathways involving "preformed" purines and pyrimidines and their derivatives. Since radioactive carbonate, glycine, and formate were not incorporated into the purines obtained from DNA, and radioactive formate only caused labeling of the thymine, it would appear unlikely that the de novo biosynthesis of purine and pyrimidine rings occurs in milts. C-1, C-6, and generally labeled glucose-14C caused labeling of the 2-deoxyribose, but not of the purines or pyrimidines, of DNA. Radioactive adenylic acid, deoxyuridylic acid, ribose 5-phosphate, and 5-phospho-α-D-ribofuranosyl-1-pyrophosphate also caused labeling of the 2-deoxyribose of DNA.

The Interactions of adenylates with allosteric citrate synthase

1979 ◽  
Vol 57 (5) ◽  
pp. 385-395 ◽  
Author(s):  
Michael M. Talgoy ◽  
Harry W. Duckworth
Keyword(s):  
Coenzyme A ◽  
Citrate Synthase ◽  
Potent Inhibitor ◽  
Sulfhydryl Group ◽  
Fluorescence Technique ◽  
Allosteric Site ◽  
Acetyl Coenzyme A ◽  
Nitrobenzoic Acid ◽  
Adenylic Acid ◽  
Derivatives Of

Evidence is presented that a number of derivatives of adenylic acid may bind to the allosteric NADH binding site of Escherichia coli citrate synthase. This evidence includes the facts that all the adenylates inhibit NADH binding in a competitive manner and that those which have been tested protect an enzyme sulfhydryl group from reaction with 5,5′-dithiobis-(2-nitrobenzoic acid) in the same way that NADH does. However, whereas NADH is a potent inhibitor of citrate synthase, most of the adenylates are activators. The best activator, ADP-ribose, increases the affinity of the enzyme for the substrate, acetyl-CoA, and saturates the enzyme in a sigmoid manner. A fluorescence technique, involving the displacement of 8-anilino-1-naphthalenesulfonate from its complex with citrate synthase, is used to obtain saturation curves for several nucleotides under nonassay conditions. It is found that acetyl-coenzyme A, coenzyme A, and ADP-ribose all bind to the enzyme cooperatively, and that the binding of each becomes tighter in the presence of KCl the activator, and oxaloacetic acid (OAA), the second substrate. Another inhibitor, α-ketoglutarate, can compete with OAA in the absence of KClbut not in its presence. The nature of the allosteric site of citrate synthase, and the modes of action of several activators and inhibitors, are discussed in the light of this evidence.

INCORPORATION OF RADIOACTIVE PURINE AND PYRIMIDINE BASES AND OF THYMIDINE INTO THE DEOXYRIBONUCLEIC ACID OF SALMON MILTS

1964 ◽  
Vol 42 (1) ◽  
pp. 51-57 ◽  
Author(s):  
H. L. A. Tarr
Keyword(s):  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine ◽  
Experimental Conditions ◽  
Live Fish ◽  
Pyrimidine Bases

C14-labeled adenine, guanine and cytosine, and tritiated thymidine were incorporated into the deoxyribonucleic acid of salmon milts, either by injection into the milts of live fish or into excised milts. The amount incorporated was very small. Under the experimental conditions radioactive nucleosides, deoxyuridine, adenosine 5′-mono- and tri-phosphates, orotic acid, uracil, ribose 1-phosphate, and ribose 5-phosphate were not incorporated. It is suggested that these results may be due to the comparative impermeability of the cells to the various compounds.

ChemInform Abstract: OXIDATION OF 1,3-DIMETHYL DERIVATIVES OF PYRIMIDINE BASES WITH M-CHLOROPERBENZOIC ACID

1984 ◽  
Vol 15 (45) ◽  
Author(s):  
T. HARAYAMA ◽  
K. KOTOJI ◽  
F. YONEDA ◽  
T. TAGA ◽  
K. OSAKI ◽  
...  
Keyword(s):  
Pyrimidine Bases ◽  
Derivatives Of
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