EVIDENCE FOR THE LYSOSOMAL NATURE OF THE PAS-POSITIVE GRANULES IN THE PURKINJE CELL OF THE CEREBELLAR CORTEX IN THE ADULT CAT

1964 ◽  
Vol 42 (1) ◽  
pp. 103-112 ◽  
Author(s):  
L. A. Chouinard
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Purkinje Cell ◽  
Cerebellar Cortex ◽  
Enzymatic Activities ◽  
Frozen Sections ◽  
Acid Hydrolases ◽  
Transparent Zone ◽  
Adult Cat ◽  
Dendritic Branches

In the adult cat, the Purkinje cell of the cerebellar cortex contains PAS-positive granules distributed more or less at random within the pericaryone and the primary dendritic branches. In the present study, evidence has been obtained indicating that such granules exhibit enzymatic activities characteristic of the cytoplasmic organelles known as lysosomes. Frozen sections of the cerebellar cortex, fixed in chilled formol–calcium and stained according to histochemical procedures for the detection of acid hydrolases, indeed reveal that the granules in question possess high levels of acid phosphatase, ribonuclease, deoxyribonuclease, and arylsulphatase activities. Further support for our conclusion comes from electron microscope observations of osmium-fixed material which show that the PAS-positive granules are solid organelles possessing ultrastructural features characteristic of the lysosomes in general. These features include a single, usually complete, limiting membrane; a 70 to 100 Å wide electron-transparent zone separating the limiting membrane from the organelle matrix proper; a matrix consisting of a fine, rather homogeneous, grainy material of moderate electron opacity which often contains ill-defined masses or clumps made up of a more electron-dispersing substance.

HISTOCHEMICAL CHARACTERISTICS OF THE PAS-POSITIVE INCLUSIONS IN THE PURKINJE CELL OF THE CEREBELLAR CORTEX IN THE ADULT CAT

1962 ◽  
Vol 40 (5) ◽  
pp. 871-878 ◽  
Author(s):  
L. A. Chouinard
Keyword(s):  
Chemical Analysis ◽  
Purkinje Cell ◽  
Physical Properties ◽  
Purkinje Cells ◽  
Cerebellar Cortex ◽  
Protein Complex ◽  
Protein Fraction ◽  
Size And Shape ◽  
Adult Cat ◽  
Dendritic Branches

A chemical analysis of the PAS-positive inclusions present in the Purkinje cell of the adult cat has been attempted utilizing various accepted histochemical procedures. Two distinct types of inclusions have been identified. The inclusions of the first type are present m the Purkinje cells of all investigated animals and appear as isolated granules distributed more or less at random within the pericaryone and the primary dendritic branches; these granules exhibit the histochemical characteristics of a neutral mucopolysaccharide. The inclusions of the second type are present in the Purkinje cells of only 9 of the 22 investigated animals and appear as granules grouped into clusters of varying size and shape usually located in the upper half portion of the pericaryone and also within the primary dendritic branches; these granules contain not only a mucopolysaccharide but also a lipid and a protein fraction. This mucopolysaccharide–lipid–protein complex exhibits the physical properties and the staining reactions of the lipofuscin pigments.

scholarly journals Lafora Bodies Associated with Neurologic Signs in a Cat

1998 ◽  
Vol 35 (3) ◽  
pp. 218-220 ◽  
Author(s):  
D. G. Hall ◽  
W. L. Steffens ◽  
L. Lassiter
Keyword(s):  
Young Adult ◽  
Purkinje Cell ◽  
Cerebellar Cortex ◽  
Affected Area ◽  
Lafora Bodies ◽  
Adult Cat ◽  
The Brain ◽  
Incidental Lesions

Lafora bodies (polyglucosan deposits) were identified in the brain of a young adult cat with neurologic signs characterized by intermittent but progressively worsening head and body tremors. The cerebellar cortex was the most severely affected area of brain, and the deposits were identified within Purkinje cell bodies and processes and throughout the neuropil. The association of Lafora bodies with neurologic signs, occurrence of deposits within neuronal perikarya, and distribution primarily within the cerebellar cortex are features distinct from the more commonly recognized situation in which Lafora bodies occur as incidental lesions in cats.

Phosphatases in the Neurones of Locusta Migratoria

1963 ◽  
Vol s3-104 (68) ◽  
pp. 475-481
Author(s):  
ROSEMARY S. LEE
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Golgi Apparatus ◽  
Acid Phosphatase Activity ◽  
Locusta Migratoria ◽  
Frozen Sections ◽  
Thiamine Pyrophosphatase ◽  
Motor Neurones ◽  
Membrane Bound ◽  
Pyrophosphatase Activity

Frozen sections of motor neurones in the thoracic ganglia of Locusta migratoria were treated for thiamine pyrophosphatase activity and for acid phosphatase activity. The TPPase-positive bodies range from 0.5 to 1.25 µ diameter and appear to be the small, membrane-bound inclusions described by Ashhurst and Chapman (1962) in their electron-microscope work; these are the smaller lipochondria of Shafiq (1953). The acid-phosphatase-positive bodies range from 1 to 2.5 µ, diameter and seem to be the lamellar aggregates described by Ashhurst and Chapman that are very similar to γ-cytomembranes, and which are the larger lipochondria of Shafiq. It is concluded that the enzyme content of the γ-cytomembranes is very different in this cell from their content in the vertebrate neurone, and doubt is thrown on the usefulness of TPPase activity as a marker for the Golgi apparatus in invertebrate tissue.

Prefertilization ovule development in Capsella: the dyad, tetrad, developing megaspore, and two-nucleate gametophyte

1986 ◽  
Vol 64 (4) ◽  
pp. 875-884 ◽  
Author(s):  
Patricia Schulz ◽  
William A. Jensen
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Acid Phosphatase ◽  
De Novo ◽  
Periodic Acid ◽  
Aniline Blue ◽  
Ovule Development ◽  
Periodic Acid Schiff ◽  
Fluorescent Material ◽  
Autophagic Vacuoles

Ovules of Capsella bursa-pastoris at the dyad and tetrad stages of meiosis and at the megaspore and two-nucleate stages of the gametophyte were studied with the electron microscope. The cells of the dyad and tetrad are separated by aniline blue fluorescent cross walls and receive all types of organelles and autophagic vacuoles that were present in the meiocyte. Autophagic vacuoles enclose ribosomes and organelles and show reaction product for acid phosphatase. Autophagic vacuoles and some plastids are absorbed into the enlarging vacuoles of the growing megaspore. Other plastids appear to survive meiosis and there is no evidence for their de novo origin. Some mitochondria appear to degenerate in the enlarging megaspore but others look healthy and there is no evidence for the de novo origin of mitochondria. The nucleolus of the developing megaspore becomes very large and the cytoplasm is extremely dense with ribosomes. The cell wall is thickened by an electron-translucent, periodic acid – Schiff negative, aniline blue fluorescent material and contains plasmodesmata that link the megaspore with the nucellus. The plasmalemma of the growing megaspore produces microvilluslike extensions into this wall that disappear with the formation of the two-nucleate gametophyte. Plasmodesmata disappear from the cell wall at the four-nucleate stage.

scholarly journals ROCK PHOSPHATE SOLUBILIZATION AND ENZYMATIC ACTIVITIES FROM INDIGENOUS BACTERIA ON CASSAVA MILL EFFLUENT CONTAMINATED SOIL

2020 ◽  
Vol 1 (2) ◽  
pp. 37-51
Author(s):  
C. E. Oshoma ◽  
S. O. Nwodo ◽  
I. S. Obuekwe
Keyword(s):  
Acid Phosphatase ◽  
Contaminated Soil ◽  
Rock Phosphate ◽  
Phosphate Solubilization ◽  
Enzymatic Activities ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Indigenous Bacteria ◽  
Rock Phosphate Solubilization ◽  
Phosphate Solubilizing

The processing of cassava into value-added products is associated with discharge of effluents which contain substances that have adverse effect on the environment. Remediative activity of indigenous bacteria can be stimulated by supplementing effluents with phosphorus. Rock phosphate (RP) solubilization and enzymatic activities from bacteria on the cassava mill effluents (CME) contaminated soil was investigated. Soil mixed with varying concentrations of CME (0, 100, 200, 300, 400, 500 and 600 ml) and 10 g of RP were analyzed on days 0 and 16. Parameters analyzed were changes in pH, heterotrophic bacteria load, phosphate-solubilizing bacteria load, available phosphorus, acid phosphatase, cellulase and urease concentrations. The results showed that the medium containing 400 ml CME contaminated soil had the highest phosphate-solubilizing bacteria load (12.60 ± 2.08 x 106 cfu/ml), available phosphorus (126.00 ± 4.08 mg/kg), acid phosphatase (9.54 ± 0.51 mgN/g/min), cellulase (15.24 ± 0.81 mg/g/6h) and urease concentration (2.15±0.22 mg/g/2h). The control had the lowest phosphate-solubilizing bacteria load and enzymatic activity. Biostimulation of indigenous bacteria to enhance the degradation of cassava mill effluent-contaminated soil, using rock phosphate, showed promising results. This implies that rock phosphate solubilization by indigenous bacteria in CME-contaminated soils could be important for the remediation and reclamation of contaminated lands.

Sign in / Sign up

Export Citation Format

Share Document