STUDIES ON THE MORPHOLOGY OF FEMALE EUDORYLAIMUS OBSCURUS (THORNE AND SWANGER, 1936) ANDRÁSSY, 1959

1962 ◽  
Vol 40 (5) ◽  
pp. 747-754 ◽  
Author(s):  
V. M. Das
Keyword(s):  
Slight Modification ◽  
Silver Impregnation ◽  
Impregnation Method ◽  
Serial Sections ◽  
Organ Systems ◽  
Cuticular Structures ◽  
Whole Mounts

The anatomy of Eudorylaimus obscurus is described from whole mounts and serial sections. The description is based on living nematodes, using intravital stains, and preserved specimens mounted in glycerine. Serial sections were prepared from specimens embedded in wax and the sections subsequently stained with haematoxylin and eosin. Cajal's silver-impregnation method with slight modification was used with useful results.In this study, particular attention is given to the cuticular structures, i.e., transverse striae, longitudinal striae, lateral fields, lateral pores, median pores, and hemizonid, which are illustrated and described. In addition to these, reproductive, excretory, and other organ systems are also described and illustrated.

MORPHOLOGICAL STUDIES OF THE MALE NYGOLAIMUS AQUATICUS THORNE, 1930

1963 ◽  
Vol 41 (5) ◽  
pp. 725-732
Author(s):  
V. M. Das
Keyword(s):  
Nervous System ◽  
Digestive System ◽  
Reproductive Organs ◽  
The Body ◽  
Excretory System ◽  
Serial Sections ◽  
Morphological Studies ◽  
Organ Systems ◽  
Cuticular Structure ◽  
Whole Mounts

The anatomy of males of Nygolaimus aquaticus Thorne, 1930, collected from Blackburn, Ontario, is described. Descriptions are based on whole mounts and also on serial sections. Specimens embedded in paraffin were cut in sections 9 μ in thickness and stained with haematoxylin and eosin.Special emphasis is given to cuticular structure, such as median pores, lateral pores, hemizonid, and transverse and longitudinal striations, which are described and illustrated. Besides these structures, other organ systems of the body, such as digestive system, excretory system, nervous system, and reproductive organs, have also been described and illustrated.

Three-Dimensional Reconstruction Using Stereo Pairs from Serial Thick Sections

1977 ◽  
Vol 35 ◽  
pp. 562-563
Author(s):  
Robert Glaeser ◽  
Thomas Bauer ◽  
David Grano
Keyword(s):  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sections ◽  
Thin Sections ◽  
3 Dimensional ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Dimensional Reconstruction ◽  
Stereo Imagery ◽  
Whole Mounts

In transmission electron microscopy, the 3-dimensional structure of an object is usually obtained in one of two ways. For objects which can be included in one specimen, as for example with elements included in freeze- dried whole mounts and examined with a high voltage microscope, stereo pairs can be obtained which exhibit the 3-D structure of the element. For objects which can not be included in one specimen, the 3-D shape is obtained by reconstruction from serial sections. However, without stereo imagery, only detail which remains constant within the thickness of the section can be used in the reconstruction; consequently, the choice is between a low resolution reconstruction using a few thick sections and a better resolution reconstruction using many thin sections, generally a tedious chore. This paper describes an approach to 3-D reconstruction which uses stereo images of serial thick sections to reconstruct an object including detail which changes within the depth of an individual thick section.

A Rapid Silver Impregnation Method for Nervous Tissue: A Modified Protargol-Peroxide Technic

1979 ◽  
Vol 54 (2) ◽  
pp. 97-100 ◽  
Author(s):  
G. P. Loots ◽  
J. M. Loots ◽  
J. M. M. Brown ◽  
J. L. Schoeman
Keyword(s):  
Nervous Tissue ◽  
Silver Impregnation ◽  
Impregnation Method

On the Anatomy of the Trematode Petasiger exaeretus Dietz 1909, from the Intestine of Phalacrocorax Carbo

Parasitology ◽  
1934 ◽  
Vol 26 (1) ◽  
pp. 133-137 ◽  
Author(s):  
Evan Davies
Keyword(s):  
Small Intestine ◽  
Type Species ◽  
Detailed Account ◽  
Serial Sections ◽  
Phalacrocorax Carbo ◽  
The Family ◽  
Whole Mounts

The genus Petasiger which belongs to the family Echinostomidae was proposed by Dietz in 1909, with Petasiger exaeretus as the type species. The description given by Dietz of this species is incomplete and it is proposed therefore to present below a more detailed account of the anatomy, based on an examination of whole mounts and serial sections. The material was obtained from the small intestine of cormorants shot on the coast of Cardigan Bay between Aberystwyth and Llanrhystyd. Five cormorants were shot in July, and of these two contained a large number of Petasiger exaeretus in the small intestine.

Observations on the function of the choriothete and on egg hatching in Glossina spp. (Dipt., Glossinidae)

1973 ◽  
Vol 62 (3) ◽  
pp. 371-374 ◽  
Author(s):  
Michael J. Roberts
Keyword(s):  
Recent Work ◽  
Instar Larva ◽  
Serial Sections ◽  
Egg Hatching ◽  
Glossina Austeni ◽  
First Instar ◽  
Muscular Tension ◽  
Eggs And Larvae ◽  
Whole Mounts

The choriothete in Glossina austeni Newst. and G. morsitans Westw. was studied from serial sections of females, from dissected material and whole mounts of eggs and larvae. The choriothete cells are secretory and, while an embryo or first-instar larva is attached to it, are not stretched. The external muscles dilate the uterus or support the uterus and embryo. There is no sign of major folding or muscular tension during dechorionation of the egg. It is concluded, in contrast to recent work, that the choriothete is an organ for the support of developing embryos. Hatching of the first-instar larvae is probably achieved by means of a labral egg tooth.

Plagiorchis peterborensis sp. n. (Trematoda:Plagiorchiidae), a parasite of Lymnaea stagnalis appressa, reared in the laboratory mouse, Mus musculus

1968 ◽  
Vol 46 (2) ◽  
pp. 135-140 ◽  
Author(s):  
Bro. Joseph Kavelaars ◽  
T. K. R. Bourns
Keyword(s):  
Life Cycle ◽  
Aedes Aegypti ◽  
Mus Musculus ◽  
Egg Size ◽  
Lymnaea Stagnalis ◽  
Laboratory Mouse ◽  
Serial Sections ◽  
Laboratory Mice ◽  
Whole Mounts

Cercariae of Plagiorchis peterborensis sp. n. were obtained from Lymnaea stagnalis appressa, and the life cycle was completed using Aedes aegypti larvae and laboratory mice as experimental hosts. Description of the adult is based upon whole mounts and serial sections of 14-day-old worms. P. peterborensis resembles most closely P. muris, but differs in stylet shape, adult dimensions, and egg size.

THE MORPHOLOGY OF THE NEMATODE RADOPHOLUS GRACILIS (DE MAN, 1880) HIRSCHMANN, 1955, PARASITIC IN ROOTS OF WILD RICE, ZIZANIA AQUATICA L.

1957 ◽  
Vol 35 (1) ◽  
pp. 75-92 ◽  
Author(s):  
K. C. Sanwal
Keyword(s):  
Vas Deferens ◽  
Neutral Red ◽  
Silver Impregnation ◽  
Nerve Ring ◽  
The Body ◽  
Impregnation Method ◽  
Sense Organs ◽  
Low Oxygen ◽  
Zizania Aquatica ◽  
De Man

The anatomy of Radopholus gracilis is described, the description being based chiefly on the study of living specimens and intravital staining with methylene blue and neutral red. Cajal's silver impregnation method and Golgi's bichromate silver method, both with slight modifications, prove useful.Cuticle striated with four longitudinal incisures; 16–18 muscle cells in anterior region; head supported by six-ridged cuticular framework with six lips and 16 sense organs; excretory system with anterior and posterior canals opening into an ampulla, all on left side of the body, and a cuticular terminal duct; ovaries opposed and outstretched; vas deferens differentiated into three regions and no central rachis in the gonads; nerve ring associated with a group of 7, 13, and 27 cells on dorsal, ventral, and lateral sides respectively; phasmid nerve supply from lateral nerves; 'hemizouid' organ present. The nematodes appear to be adapted to an aquatic environment and seem to be unaffected by low temperatures and low oxygen content in their habitat.

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