THE EFFECT OF GONADAL HORMONES ON THE NUCLEIC ACID CONTENT OF LIVER AND SERUM IN THE IMMATURE PULLET, AND THE DIFFERENCE BETWEEN THE NUCLEIC ACID CONTENT OF THE LIVERS OF SEXUALLY MATURE PULLETS AND COCKERELS

1951 ◽  
Vol 29 (4) ◽  
pp. 265-275 ◽  
Author(s):  
R. H. Common ◽  
D. G. Chapman ◽  
W. A. Maw
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Testosterone Propionate ◽  
Liver Weight ◽  
Reproductive Activity ◽  
Estradiol Benzoate ◽  
Gonadal Hormones ◽  
Nucleic Acid Content ◽  
Activity Data ◽  
Young Chicken

Treatment of sexually immature pullets with testosterone propionate so as to evoke changes in combs and wattles similar to normal puberal changes did not affect liver weight or liver content of nucleic acids. Estradiol benzoate, or estradiol benzoate plus testosterone propionate, increased liver weight and total liver pentose nucleic acid, and slightly increased liver desoxypentose nucleic acid. Chemical evidence is adduced in support of the view that the increase of liver crude protein caused by estrogen is a consequence of cellular hypertrophy accompanied by a slight degree of hyperplasia. The ratio RNAP: DNAP was relatively high in the liver of the young chicken, but declined somewhat during the first 12 weeks. With the onset of reproductive activity, the ratio RNAP: DNAP increased in the livers of the females but did not show a similar tendency in the males. The results suggest that there is a sexual differentiation in the nucleic acid content of the livers of the mature fowl, and that this is reasonably attributable to endogenous estrogen activity. Data are presented in confirmation of the reported effect of estrogen in increasing serum or plasma nucleic acid in the fowl, androgen being without any such effect.

SOME EFFECTS OF PROGESTERONE ON THE RESPONSE OF THE IMMATURE PULLET TO ESTROGEN, AND SOME OBSERVATIONS ON THE EFFECTS OF GONADAL HORMONES ON THE AVIAN SPLEEN AND KIDNEY

1952 ◽  
Vol 30 (4) ◽  
pp. 201-207 ◽  
Author(s):  
W. E. J. Phillips ◽  
R. H. Common ◽  
W. A. Maw
Keyword(s):  
Nucleic Acid ◽  
Calcium Level ◽  
Serum Calcium Level ◽  
Crude Protein ◽  
Testosterone Propionate ◽  
Liver Weight ◽  
Significant Degree ◽  
Gonadal Hormones ◽  
Spleen Weight ◽  
Kidney Weight

Progesterone depressed the hypertrophy of the immature pullet's oviduct evoked by estrogen, but did not significantly affect the responses of liver weight, total liver crude protein, and serum calcium level evoked by estrogen. These effects are in some respects the converse of previously recorded effects of thiour-acil on the response of the pullet to estrogen. Progesterone did not affect the increases of liver pentose nucleic acid and desoxypentose nucleic acid evoked by estrogen. Testosterone propionate by itself, at the levels used, did not affect the amounts or ratios of liver pentose nucleic acid and desoxypentose nucleic acid. Estrogen reduced spleen weight to a highly significant degree, and progesterone significantly reduced this effect of estrogen. Testosterone did not affect spleen weight. In the experiment described estrogen increased kidney weight to a highly significant degree; progesterone tended to reduce this effect, but the tendency did not attain significance at P = 0.05.

scholarly journals Nucleic Acid Content in Tissue of the Fish N. notopterus Collected from Different Aquatic Bodies

2014 ◽  
Vol 28 ◽  
pp. 17-26
Author(s):  
K. Ravikiran ◽  
R.S. Kulkarni
Keyword(s):  
Nucleic Acid ◽  
Freshwater Fish ◽  
Acid Content ◽  
Reproductive Activity ◽  
Nucleic Acid Content ◽  
Environmental Condition ◽  
Physiochemical Parameters ◽  
Favorable Environmental Condition ◽  
Notopterus Notopterus ◽  
Growth Distribution

Nucleic acid content in brain, liver, kidney and gonads of the freshwater fish Notopterus notopterus collected from different aquatic bodies were studied during pre-spawning phase of the reproductive cycle. The three different aquatic bodies selected were Saradgi nala, Bennithora River and Bheema River and their physiochemical parameters also studied. The present study of nucleic acid content in brain, liver, kidney and gonads of the fish Notopterus notopterus collected from aquatic body Bennithora River shows normal levels of the nucleic acid as compared to that of fish from other two aquatic bodies. The normal content of nucleic acid content of N.notoptuers in the aquatic body Bennithora may be due to favorable environmental condition for survival, growth, distribution and reproductive activity.

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

Age-Related Effect of Dietary Nucleotides on Liver Nucleic Acid Content in Rats

1997 ◽  
Vol 41 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Ana T. López-Navarro ◽  
Angel Gil ◽  
Antonio Sánchez-Pozo
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Related Effect ◽  
Age Related ◽  
Dietary Nucleotides

scholarly journals Effect of starvation and refeeding on polyamine concentrations and ornithine decarboxylase antizyme in mammary gland of lactating rats

1983 ◽  
Vol 212 (1) ◽  
pp. 149-153 ◽  
Author(s):  
M E Brosnan ◽  
R Farrell ◽  
H Wilansky ◽  
D H Williamson
Keyword(s):  
Mammary Gland ◽  
Nucleic Acid ◽  
Ornithine Decarboxylase ◽  
Acid Content ◽  
Marked Decrease ◽  
Mammary Glands ◽  
Nucleic Acid Content ◽  
Decarboxylase Activity ◽  
Ornithine Decarboxylase Activity ◽  
Polyamine Content

Starvation caused a marked increase in putrescine content in mammary gland of lactating rats, together with a marked decrease in activity of ornithine decarboxylase and appearance of measurable ornithine decarboxylase antizyme. 2. Refeeding for 5 h caused disappearance of free antizyme and ornithine decarboxylase activity returned to the value in fed animals. Putrescine concentration remained elevated. 3. There was no significant change in nucleic acid content of mammary gland from starved rats, but spermidine and spermine contents increased significantly. 4. Refeeding for 5 h returned the spermidine content of mammary glands to ‘fed’ values, and significantly decreased the content of spermine, although it did not reach control values. Thus changes in polyamine content of mammary gland in starved rats are clearly dissociated from changes in either RNA content or activities of polyamine-synthetic decarboxylases. 5. Starvation caused a fall in the content of spermidine in liver, with no change in spermine content. Refeeding for 5 h returned the spermidine content to ‘fed’ values.

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