Validation of otolith daily increments in the tropical eel Anguilla celebesensis

2000 ◽  
Vol 78 (6) ◽  
pp. 1078-1084 ◽  
Author(s):  
Takaomi Arai ◽  
Daniel Limbong ◽  
Katsumi Tsukamoto
Keyword(s):  
Age Determination ◽  
Structural Features ◽  
Reference Mark ◽  
Increment Width ◽  
Daily Increments ◽  
Otolith Increments ◽  
Glass Eels ◽  
Daily Deposition ◽  
Tropical Eel ◽  
Alizarin Complexone

To validate daily increments in otoliths of the tropical freshwater eel Anguilla celebesensis, a time-reference mark was incorporated in the otoliths of glass eels by immersing the eels in alizarin complexone (ALC) solution. They were subsequently reared in aquaria for 10 or 20 days, and the increments outside a fluorescent red ALC mark were counted. The numbers from the 10- and 20-d experiments were 9.4 ± 0.9 (mean ± SD) and 19.4 ± 1.4, respectively, suggesting daily deposition of otolith increments. Precise examination of otolith microstructure from the core to the edge showed gradual changes in increment width with no discontinuous structural features, suggesting that increments were deposited continuously from hatching to time of sacrifice. It was thus concluded that otolith increments in this species can be used for daily age determination.

scholarly journals Species composition of glass eels (Anguilla spp.) recruiting to the Palu River, Central Sulawesi

2018 ◽  
Author(s):  
Novalina Serdiati ◽  
Samliok Ndobe ◽  
Abigail Moore ◽  
Deddy Wahyudi
Keyword(s):  
Species Composition ◽  
Dna Analysis ◽  
Temporal Patterns ◽  
Clove Oil ◽  
Analysis Method ◽  
Substantial Variation ◽  
Anguilla Marmorata ◽  
Glass Eels ◽  
Central Sulawesi ◽  
Tropical Eel

Demand for tropical eel seed has been increased and many tropical eel populations are under pressure. To conserve eel biodiversity and manage eel populations sustainably, it is necessary to identify eel species and their recruitment patterns at regional and watershed scales. The research objective was to determine the species composition and temporal recruit-ment patterns of glass eels recruiting to Palu River in Central Sulawesi. Glass eels sampling were conducted in January-April 2009, May-November 2010 and April-December 2011. Identification under anaesthetic (15-17.5 ppm clove oil solution) was based mainly on the number of ano-dorsal vertebrae (ADV). Species composition was dominated by two commercially species, Anguilla marmorata and A. bicolor pacifica with substantial variation and no clear temporal patterns. Specimens of other species that important from conservation and biodiversity aspects were present at each month but cannot be accurately identified using the ADV method. DNA analysis method is required to identify these specimens.

scholarly journals Age, growth, and condition of European eel (Anguilla anguilla) from six lakes in the River Havel system (Germany)

2007 ◽  
Vol 64 (7) ◽  
pp. 1414-1422 ◽  
Author(s):  
Janek Simon
Keyword(s):  
Growth Rate ◽  
Condition Factor ◽  
Energy Content ◽  
Anguilla Anguilla ◽  
European Eel ◽  
Annual Increment ◽  
Otolith Increments ◽  
Gross Energy ◽  
Glass Eels ◽  
Mean Annual Increment

Abstract Simon, J. 2007. Age, growth, and condition of European eel (Anguilla anguilla) from six lakes in the River Havel system (Germany). – ICES Journal of Marine Science, 64: 1414–1422. A total of 199 female yellow European eels (Anguilla anguilla), 21.6–66.2 cm long and 3–14 years old, was collected by electro-fishing from six lakes in the River Havel system (Germany) in spring 2001. The condition and the growth rate, estimated by otolith increments, varied between eels within single lakes and between lakes. Fulton's condition factor ranged from 0.10 to 0.24 and the gross energy content varied between 4.3 and 15.3 MJ kg−1. There were no significant differences in mean condition factor (0.16–0.18) or gross energy content (6.5–9.3 MJ kg−1) between lakes. Fastest growth was in Lake Blankensee (mean 5.3 cm year−1), and the slowest in Lake Sacrow (mean 4.0 cm year−1). For all lakes combined, the overall mean annual increment was estimated to be 4.5 cm year−1. The biggest annual increment on the otoliths was generally laid down during the first and second years in fresh water, when the growth rate was 6.1–8.5 cm year−1. Then, in the subsequent 12 years, the annual increment remained almost constant or decreased slightly (with lake-dependent values of between 1.6 and 6.8 cm year−1). In the River Havel system, the time between stocking of the lakes with glass eels and the recapture of eels at 45 cm body length was 7–10 years. The physiologically possible maximum length (L∞ values) of eels lay in the range 50–130 cm. In comparison with previous investigations (between the 1950s and the 1970s), the only difference observed was a trend towards slower growth.

scholarly journals Validation of otolith daily increments in the tropical eel Anguilla marmorata

2001 ◽  
Vol 220 ◽  
pp. 291-294 ◽  
Author(s):  
HY Sugeha ◽  
A Shinoda ◽  
M Marui ◽  
T Arai ◽  
K Tsukamoto
Keyword(s):  
Anguilla Marmorata ◽  
Daily Increments ◽  
Tropical Eel

scholarly journals DISTRIBUTION OF TROPICAL EEL GENUS Anguilla IN INDONESIA WATER BASED ON SEMI-MULTIPLEX PCR

2012 ◽  
Vol 7 (2) ◽  
pp. 139 ◽  
Author(s):  
Melta Rini Fahmi ◽  
Laurent Pouyaud ◽  
Patrick Berrebi
Keyword(s):  
Morphological Characters ◽  
Wide Distribution ◽  
Sample Collection ◽  
Fish Size ◽  
Distribution Ranges ◽  
Pcr Method ◽  
Glass Eels ◽  
Very High ◽  
Tropical Eel ◽  
Tropical Eels

Tropical eels living in Indonesian waters are known to be composed of several species, but their real listing together with their distribution ranges need to be established. The main difficulties are the very high number of islands with perennial rivers where these species are living during the growth phase of their life cycle. It is difficult, sometimes impossible, to determine the species using morphological characters, moreover on glass eels. In order to establish the geographic distribution of tropical eels of the genus Anguilla in Indonesian waters, a total 1,115 specimens were collected between 2008 and 2012. Sample collection was done in the growth habitats that are rivers and estuaries by commercial nets of different categories according to the fish size. All samples were identified genetically using the recently developed semimultiplex PCR method. We recognized four species and subspecies with wide distribution: Anguilla bicolor bicolor, Anguilla bicolor pacifica, Anguilla marmorata, and Anguilla interioris; two species with limited distribution, close to endemism: Anguilla celebesensis and Anguilla borneensis and one subspecies Anguilla nebulosa nebulosa that is only spread in river flowing into Indian Ocean.

Otolith microstructure of the Russian sturgeon, Acipenser gueldenstadti

2002 ◽  
Vol 82 (4) ◽  
pp. 679-680
Author(s):  
Takaomi Arai ◽  
Nobuyuki Miyazaki
Keyword(s):  
Caspian Sea ◽  
Age Determination ◽  
Russian Sturgeon ◽  
Otolith Microstructure ◽  
The Caspian Sea ◽  
Otolith Increments ◽  
Anterior Side

Otolith microstructure of the Russian sturgeon, Acipenser gueldenstadti was examined in adult samples collected in the Caspian Sea. The microstructure of the otolith was the same among specimens. The anterior side of the sagitta grew in two directions elongating both the long and short arms, and the long arm sections exhibited 14–21 distinct translucent and opaque zones. Otolith increments in this species can be used for age determination.

Effect of food availability on the growth and age determination of European sardine (Sardina pilchardus Walbaum, 1792) larvae

2021 ◽  
pp. 1-11
Author(s):  
Susana Garrido ◽  
Susana Ferreira ◽  
Claudia Soares ◽  
Isabel Meneses ◽  
Nuria Baylina ◽  
...  
Keyword(s):  
Environmental Variability ◽  
Stock Assessment ◽  
Larval Growth ◽  
Age Determination ◽  
Age And Growth ◽  
Vital Rates ◽  
Sardina Pilchardus ◽  
Feeding Rates ◽  
Growth Increments ◽  
Daily Deposition

Abstract Accurate assessment of age and growth of fish is essential to understand population dynamics, namely for age-structured stock assessment and for determining vital rates of fish (e.g. age at sexual maturity) and their relationship with environmental variability. To validate the daily deposition in the otoliths of European sardine (Sardina pilchardus) larvae, these were reared with a range of food densities translating into feeding rates extending from less than required for maintenance to saturated feeding levels. When exogenous feeding began, a high variability in the relationship between number of growth increments (GI) and elapsed days after hatching (dph) was observed, irrespective of the feeding treatment. GI counts using a light microscope were less than one per day for larvae <15 dph (ranging from 0.17–0.43 GI day−1) and similar for larvae reared with different food concentrations. The rate of GI count vs age was significantly higher for larvae older than 15 dph. GI count from 3–30 dph was lower than one per day (0.45–0.75 GI day−1 95% CI) for fed larvae 3–30 dph. Scanning electron microscopy confirmed the daily deposition of GI and revealed some increment widths around 0.2 μm, particularly during the first week post-hatch. Otoliths of wild sardine larvae exhibited narrow GI (<0.5 μm) comparable with reared larvae. This study demonstrates that during the first weeks after hatch the daily increment deposition is underestimated using traditional light microscopy, which must be taken into account in future works determining wild sardine larval growth.

Validation of otolith daily increments in the tropical eel Anguilla celebesensis

2000 ◽  
Vol 78 (6) ◽  
pp. 1078-1084 ◽  
Author(s):  
Takaomi Arai ◽  
Daniel Limbong ◽  
Katsumi Tsukamoto
Keyword(s):  
Daily Increments ◽  
Tropical Eel

Daily incuremtns in Otoliths of juvenile Snapper, Pagrus auratus (Sparidae)

1992 ◽  
Vol 43 (5) ◽  
pp. 1015 ◽  
Author(s):  
MP Francis ◽  
MW Williams ◽  
AC Pryce ◽  
S Pollard ◽  
SG Scott
Keyword(s):  
Population Dynamics ◽  
New Zealand ◽  
Light Microscope ◽  
Wild Population ◽  
Larval Period ◽  
Increment Width ◽  
Daily Increment ◽  
Daily Increments ◽  
Pagrus Auratus ◽  
Increment Formation

Sagitta microstructure was investigated in juvenile New Zealand snapper, Pagrus auratus, to provide a basis for daily ageing in population dynamics studies. Transverse sections produced the clearest daily increments and were the easiest to prepare. Daily increment formation was validated up to an age of 100 days by using increment counts from reared juveniles and up to about 160 days by using changes in mean increment counts from juveniles sampled from a wild population at different times. A prominent metamorphic mark was visible in transverse and frontal sections, providing a means for determining the duration of the larval period, and juvenile postmetamorphic ages. Postmetamorphic increment width varied with the age of the snapper and with season. Increment width dropped below 0.5 μm in winter and increments could not be resolved with a light microscope.

Interpretation of statolith microstructure in reared hatchling paralarvae of the squid Illex argentinus

2004 ◽  
Vol 55 (4) ◽  
pp. 403 ◽  
Author(s):  
Mitsuo Sakai ◽  
Norma Brunetti ◽  
Marcela Ivanovic ◽  
Beatriz Elena ◽  
Kazuyoshi Nakamura
Keyword(s):  
Full Range ◽  
Increment Width ◽  
Daily Growth ◽  
Daily Increments ◽  
Growth Increments ◽  
Absorption Phase ◽  
Yolk Absorption ◽  
Different Temperatures ◽  
Illex Argentinus ◽  
Two Phases

To identify sub-daily or aperiodic increments of statolith growth in the ommastrephid squid Illex argentinus, we examined statolith microstructure, especially with regard to the natal ring, where counting of daily growth increments should begin, and the widths of subsequent daily increments. Paralarvae obtained by artificial fertilisation were incubated on board at different temperatures ranging from 11.4 to 25.4°C, and were starved throughout the experiments. We observed statolith growth from newly hatched to 10-day-old paralarvae and used alizarine complexone staining to attempt validation of the growth. The maximum statolith radius (MSR) of newly hatched paralarvae was constant at 21.1 μm across the full range of temperatures, with the exception of 25.4°C. Daily growth of MSR was analysed separately in two phases, the pre-yolk-absorption phase (i.e. yolk sac still present) and the post-yolk-absorption phase. During the pre-yolk-absorption phase, the daily growth rate (DGR, y) of the MSR varied from 3 to 7 μm day–1 depending on rearing temperature (x) and was expressed as y = 0.37x – 1.77. We concluded that the natal ring forms at 21 μm MSR. The initial increment width obtained from the DGR of MSR seems applicable for distinguishing daily rings from sub-daily rings, although this application should be limited to hatchling paralarvae in the pre-yolk-absorption phase.

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