Prorenin is present in human red blood cells

1991 ◽  
Vol 69 (9) ◽  
pp. 1394-1397 ◽  
Author(s):  
C. Troffa ◽  
G. Tonolo ◽  
P. Manunta ◽  
A. Pazzola ◽  
A. Soro ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Blood Cells ◽  
Normal Subjects ◽  
Trypsin Treatment ◽  
Angiotensin I ◽  
Hypertensive Patients ◽  
Human Red Blood Cells ◽  
Before And After ◽  
Inactive Renin ◽  
Homologous Substrate

We looked for the presence of prorenin in erythrocytes from normal subjects (n = 8), hypertensive patients (n = 8), and pregnant women (n = 8). Angiotensin I generation was measured at 37 °C, pH 5.7, in the presence of homologous substrate (1400 ng/mL) before and after trypsin activation (100 μg/mL) in (A) haemolyzed erythrocytes, (B) supernatants of haemolyzed erythrocytes, and (C) in the sixth washing of erythrocytes diluted 1:1 with a 0.1 M Tris buffer containing 0.5% bovine serum albumin and protease inhibitors. Haemolyzed erythrocytes generated angiotensin I only after trypsin treatment, and the rate of generation was the same (A) before and (B) after centrifugation at 20000g, indicating the absence of prorenin bound to the cell membranes. When aliquots of the last washing of erythrocytes (C) were tested for angiotensin I generation before and after trypsin, they did not generate angiotensin I, indicating that residual prorenin from the plasma was no longer present in our preparation. Angiotensin I generation by trypsin-treated A and B was completely abolished by preincubation with anti-renin serum. The level of prorenin was not significantly different in the erythrocytes from normal, hypertensive, and pregnant subjects (68 ± 10, 58 ± 7 and 107 ± 17 pg angiotensin I∙mL−1∙h−1, ns) in spite of their very different plasma levels (21 ± 2.5, 17 ± 2.4 and 110 ± 12 ng angiotensin I∙mL−1∙h−1, p < 0.01 for pregnant women compared with both normal and hypertensive subjects). Our data show that prorenin is present in human erythrocytes in fairly constant and clearly detectable amounts, thus suggesting a possible intracellular role for it.Key words: inactive renin, intracellular prorenin, erythrocytes, prorenin.

scholarly journals Pengaruh Pemberian Buah Pisang Mas Terhadap Peningkatan Kadar Hb Pada Ibu Hamil Trimester III

2019 ◽  
Vol 5 (2) ◽  
pp. 50-54
Author(s):  
Elsa Kumala Safitri ◽  
Miftahul Mualimah ◽  
Herliana Riska
Keyword(s):  
Data Analysis ◽  
Pregnant Women ◽  
Blood Cells ◽  
Research Method ◽  
Observation Data ◽  
Banana Fruit ◽  
Signed Rank ◽  
Before And After ◽  
Protein Rich ◽  
The Given

Hemoglobin is a protein-rich substance bezi, Have affinity (power team) against oxygen and with oxygen it forms a oxihemoglobin in red blood cells. The goal in this research is to know the influence of banana fruit mas to increased hemoglobin on pregnant women trimester III in BPM Ny "B" Ngasem Regency Kediri Year 2017. In this research method using alphabets experiment and approach Pre alphabets experiment with Purposive Sampling. This research was conducted in BPM Ny "B" Ngasem Regency Kediri Year 2017. Instruments in use are sheets of observation, data analysis with the iji Wlcoxon Signed Rank. Results of research of banana fruit mas on pregnant women this trimester III that the magnitude of the value of ρ is less than the value of 0.000 α = 0.05 (0.000 < 0.05) so that H0 is rejected and the H1 is accepted means there is influence of hemoglobin levels before and after the given bananas mas against peningktan the levels of haemoglobin in pregnant women Trimester III in BPM Ny. " B “ Ngasem Regency Kediri year 2017. With this expected to consume fruit banana mas on pregnant women may help lower III trimester anemia with practical.

scholarly journals Effect of Halothane and Enflurane Anaesthesia on the Level of Reduced Glutathione in Human Red Blood Cells

1980 ◽  
Vol 8 (3) ◽  
pp. 356-358 ◽  
Author(s):  
N. S. Agar ◽  
G. B. H. Lewis
Keyword(s):  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Reduced Glutathione ◽  
Haemoglobin Concentration ◽  
Red Cell ◽  
Anaesthetic Agents ◽  
Human Red Blood Cells ◽  
Enflurane Anaesthesia ◽  
Before And After

No significant changes were found in packed cell volume, haemoglobin concentration and red cell glutathione levels in patients before and after anaesthesia with halothane or enflurane. These results, though unable to explain the mechanism, support the earlier suggestion that glutathione plays little, if any, role in protecting liver against toxic effects of these anaesthetic agents or their metabolites.

Catechol O-Methyltransferase in Red Blood Cells of Schizophrenic, Depressed, and Normal Human Subjects

1976 ◽  
Vol 128 (2) ◽  
pp. 184-187 ◽  
Author(s):  
Helen L. White ◽  
Malcolm N. McLeod ◽  
Jonathan R. T. Davidson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Human Subjects ◽  
Normal Subjects ◽  
Enzyme Preparations ◽  
Human Red Blood Cells ◽  
Schizophrenic Patients ◽  
Normal Human ◽  
The Mean ◽  
Isoelectric Ph

SummaryCatechol O-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, S-adenosyl-L-methionine and 3,4-dihydroxybenzoic acid. The mean enzyme activity found in 24 normal subjects was 29.2 nmol/hr/ml RBC. The mean activity in blood of 33 female unipolar depressives was not significantly different from normal. However, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38.9 nmol/hr/ml RBC). Partially purified enzyme preparations from blood of normal and schizophrenic individuals were indistinguishable with respect to substrate specificities, isoelectric pH values, and ratios of the two O-methylated products. Therefore it is unlikely that any defect in O-methylation which may occur in schizophrenia can be attributed to a change in the intrinsic properties of erythrocyte catechol O-methyltransferase.

THE EFFECT OF NIACIN ON THE PYRIDINE NUCLEOTIDE CONTENT OF HUMAN BLOOD CELLS IN ANEMIA

1950 ◽  
Vol 28e (3) ◽  
pp. 97-105
Author(s):  
M. C. Blanchaer ◽  
D. E. Bergsagel ◽  
Pamela Weiss ◽  
Dorothy E. Jefferson
Keyword(s):  
Nicotinic Acid ◽  
Blood Cells ◽  
Normal Subjects ◽  
Pyridine Nucleotide ◽  
Nucleotide Content ◽  
Human Blood Cells ◽  
Normal Individuals ◽  
Before And After ◽  
Niacin Therapy ◽  
Normal Controls

The pyridine nucleotide content of the blood cells (PN/BG) of four normal individuals and five anemic patients was measured repeatedly before and after supplementing the diet with niacin. Daily doses of 50 mgm. nicotinic acid or nicotinamide failed to affect the values in the normal subjects and two well-nourished anemic patients. The same dose of nicotinic acid rapidly raised the PN/BC of three poorly nourished anemic patients to values approximating those of well-nourished subjects with similar degrees of anemia. The results obtained after saturation with niacin confirmed the previous report that the PN/BC is higher in anemic patients than in normal controls and that a negative correlation exists between the pyridine nucleotide values and the severity of the anemia. In spite of continued niacin therapy, correction of the anemia was accompanied by a gradual decrease in the values until they approached those of the normal subjects. The present findings also confirm the earlier report that changes in the PN/BC are apparently independent of moderate variations in the number of circulating leucocytes and reticulocytes and bear no relationship to the size or hemoglobin content of the red cells. The significance of these findings in relation to human niacin nutrition is discussed.

EFFECT OF INHIBITION OF CONVERTING ENZYME ON INACTIVE RENIN IN THE CIRCULATION OF SALT-REPLETE AND SALT-DEPLETE NORMAL SUBJECTS

1980 ◽  
Vol 86 (2) ◽  
pp. 329-335 ◽  
Author(s):  
J. A. MILLAR ◽  
M. T. HAMMAT ◽  
C. I. JOHNSTON
Keyword(s):  
Angiotensin Ii ◽  
Normal Subjects ◽  
Inhibitory Influence ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Active Inhibitor ◽  
Active Renin ◽  
Angiotensin I Converting Enzyme ◽  
Inactive Renin ◽  
Orally Active

Angiotensin II exerts an inhibitory influence on active renin release from the kidney. To assess a possible role for angiotensin II in the release of inactive renin, levels in the circulation were measured before and at regular intervals after the administration of captopril, an orally active inhibitor of angiotensin I-converting enzyme, to 12 salt-replete and six salt-deplete normal subjects. Concurrent measurements of active renin, angiotensin I and angiotensin II were also performed. Basal inactive renin in the salt-deplete group was increased compared with the salt-replete subjects, but inactive renin remained constant in both groups after treatment with captopril. There were significant increases in concentrations of both active renin and angiotensin I after treatment with captopril in all subjects and corresponding decreases in angiotensin II. These results suggested that angiotensin II does not influence the release of inactive renin, in contrast with its role in the release of active renin.

scholarly journals KONSUMSI RUMPUT LAUT DAPAT MENGATASI ANEMIA KEHAMILAN

2020 ◽  
Vol 6 (1) ◽  
pp. 68-74
Author(s):  
Melly Damayanti
Keyword(s):  
Pregnant Women ◽  
Blood Cells ◽  
White Blood Cells ◽  
Third Trimester ◽  
Work Area ◽  
Before And After ◽  
The Status ◽  
Post Test ◽  
Anemia In Pregnancy ◽  
In Pregnancy

  ABSTRACT Anemia in pregnancy is a condition in pregnant women with hemoglobin levels less than 11gr% during pregnancy. An increased of anemia often occurs in the third trimester of pregnancy. Pregnant women needs to be supported with nutritional patterns which contain some necessary intermediate in the synthesis of hemoglobin, which  is seaweed. Seaweed type Euchoma sp contains iron, a mixture of which is needed in the synthesis of hemoglobin, has a high bioavailability of substances and is able to stabilize the number of red blood cells, white blood cells, and hemoglobin. The purpose of the study was to determine the status of anemia before and after the study. The study design is the pre-post test design. The population of the study was 30 third trimester pregnant women in the Tanjung Pinang City Primary Health Care Work Area. Respondents were given seaweed as much as 200 gram for 7 days and the 8th day repeated hemoglobin levels were measured. After being given an intervention, there was an increase in hemoglobin levels in pregnant women by 1.2gr% and there were no more respondents suffering anemia.   Keywords : consumption of seaweed, anemia in pregnancy

Enzymatic Activity of Renin in Plasma of Normal and Uraemic Subjects

1984 ◽  
Vol 67 (3) ◽  
pp. 365-368 ◽  
Author(s):  
Theodore A. Kotchen ◽  
Tam T. Guyenne ◽  
Pierre Corvol ◽  
Joel Menard
Keyword(s):  
Renal Failure ◽  
Enzymatic Activity ◽  
Plasma Renin ◽  
Normal Subjects ◽  
Renin Activity ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Inactive Renin ◽  
Substrate Concentrations

1. Plasma renin reactivity (PRR) is the rate of angiotensin I production after addition of renin to plasma, minus endogenous renin activity. PRR is increased in plasma of patients with renal failure compared with that of normal subjects. The present study was carried out to determine if increased PRR in uraemic plasma is related to differences of endogenous active or inactive renin, endogenous renin substrate, or pH of the incubation in vitro. 2. PRR in plasma of ten uraemic patients was greater (P<0.02) than that in plasma of ten normal subjects in incubations carried out at pH 7.4 and 5.7. 3. Increased PRR was not accounted for by differences of endogenous active and inactive renin activity. 4. After addition of renin, renin concentration (measured by direct radioimmunoassay) did not differ in normal and uraemic plasma. 5. Renin substrate concentration, measured both indirectly and by direct radioimmunoassay, also did not differ in normal and uraemic plasma. 6. Increased PRR in uraemic plasma is not related to alterations of renin or renin substrate concentrations. These observations are consistent with our earlier hypothesis that there is a deficiency of a renin inhibitor in uraemic plasma.

The Conversion of [35S]PTC-Angiotensin I to PTC-Angiotensin II in Plasma of Normotensive and Hypertensive Subjects

1970 ◽  
Vol 38 (2) ◽  
pp. 217-223 ◽  
Author(s):  
E. C. Osborn ◽  
N. G. Hodges ◽  
P. T. Pickens ◽  
P. R. Willicombe ◽  
R. F. Mahler
Keyword(s):  
Angiotensin Ii ◽  
Normal Subjects ◽  
The Other ◽  
Angiotensin I ◽  
Hypertensive Patients

1. The rate of conversion of angiotensin I to angiotensin II was measured by using their [35S]PTC-analogues which can be separated and identified by radiochromatography. 2. There was no difference in the rate of conversion of one compound to the other in the plasma of normal subjects and hypertensive patients.

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