Regulation of hepatic cytochrome P-450 during Infectious disease

1990 ◽  
Vol 68 (6) ◽  
pp. 777-781 ◽  
Author(s):  
Kenneth W. Renton ◽  
Leah C. Knickle
Keyword(s):  
Infectious Disease ◽  
Viral Infections ◽  
Oligonucleotide Probe ◽  
Protein S ◽  
Unique Sequence ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Mixed Function Oxidase System ◽  
Cytochrome P 450 ◽  
Hepatic Cytochrome

During episodes of infectious disease the mixed function oxidase system is depressed and the capacity of the liver to metabolize drags can be compromised in both animals and humans. The depression that occurs during viral infections is mediated via the production of interferon. This action of interferon requires the synthesis of an intermediate protein(s) yet to be identified. Using an oligonucleotide probe for a unique sequence in cytochrome P-450LAω we have now shown that the mRNA for this isozyme is depressed following the administration of interferon inducers. The magnitude in the loss of mRNA corresponds to the magnitude of the loss in the levels of this isozyme. This depression is observed within 6 h of interferon exposure. It is concluded that the decrease in drag metabolism during viral infections is caused by an interferon-mediated loss in mRNA and subsequent cytochrome P-450 synthesis in the liver.Key words: cytochrome P-450, drug metabolism, mixed function oxidase, interferon, viral infection.

Occurrence of a cytochrome P-450-containing mixed-function oxidase system in the pond snail,Lymnaea stagnalis

Xenobiotica ◽  
1991 ◽  
Vol 21 (2) ◽  
pp. 223-233 ◽  
Author(s):  
M. Wilbrink ◽  
E. J. Groot ◽  
R. Jansen ◽  
Y. De Vries ◽  
N. P. E. Vermeulen
Keyword(s):  
Lymnaea Stagnalis ◽  
Pond Snail ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Mixed Function Oxidase System ◽  
Cytochrome P 450

Use of monoclonal antibodies to characterize the induction response of the cytochrome P-450-dependent mixed function oxidase system to nitrofluoranthenes

1987 ◽  
Vol 8 (11) ◽  
pp. 1679-1684 ◽  
Author(s):  
Wasiuddin A. Khan ◽  
Parthasarathy Asokan ◽  
Sang S. Park ◽  
Harry V. Gelboin ◽  
David R. Bickers ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Mixed Function Oxidase System ◽  
Cytochrome P 450

scholarly journals Mixed function oxidases in response to different types of dietary lipids in rats

1990 ◽  
Vol 63 (2) ◽  
pp. 249-257 ◽  
Author(s):  
Morio Saito ◽  
Akira Oh-Hashi ◽  
Mika Kubota ◽  
Eiichi Nishide ◽  
Michio Yamaguchi
Keyword(s):  
Soya Bean ◽  
Dietary Lipids ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Sardine Oil ◽  
Demethylase Activity ◽  
Mixed Function Oxidase System ◽  
Mixed Oil ◽  
Cytochrome P 450 ◽  
Microsomal Mixed Function Oxidase

The influence of dietary lipids on the liver microsomal mixed function oxidase system and on pentobarbital-induced sleeping time was studied in rats. Giving diets containing (g/kg) 150 olive oil, 150 lard or 150 soya-bean oil for 21 d (Expt 1) increased the cytochrome P-450 content in the order: olive oil < lard < soya-bean oil. When diets containing (g/kg) 150 lard, 150 soya-bean oil, 150 sardine oil or an equal mixture of 50 of each oil were given for 15 d (Expt 2), the cytochrome P-450 content and aminopyrine N-demethylase activity were significantly higher in the sardine-oil and mixed-oil groups than in the lard group, and the activity of aminopyrine N-demethylase was also significantly higher in the soya-bean oil group compared with the lard group. A significantly higher activity of NADPH-cytochrome c reductase (EC 1.6.2.5) was observed in the sardine-oil group than in the other three groups. Aniline hydroxylase activity and cytochrome b5 content remained unchanged in all the groups. Pentobarbital-induced sleeping time measured on day 15 (Expt 2) varied inversely with the changes in cytochrome P-450 content and aminopyrine N-demethylase activity in the three single-fat groups, but not in the mixed-oil group, reflecting liver microsomal metabolic activity for pentobarbital in vivo. From these results, it appears that high intakes of polyunsaturated fatty acids (18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3) stimulate the liver microsomal mixed function oxidase system.

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