Studies on the microsomal mixed-function oxidase system: mechanism of action of hepatic NADPH-cytochrome P-450 reductase

Biochemistry ◽  
1981 ◽  
Vol 20 (7) ◽  
pp. 1722-1730 ◽  
Author(s):  
Takashi Iyanagi ◽  
Ryu Makino ◽  
F. Koichi Anan
Keyword(s):  
Mechanism Of Action ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Nadph Cytochrome ◽  
Mixed Function Oxidase System ◽  
Cytochrome P 450 ◽  
Microsomal Mixed Function Oxidase ◽  
System Mechanism

scholarly journals Mixed function oxidases in response to different types of dietary lipids in rats

1990 ◽  
Vol 63 (2) ◽  
pp. 249-257 ◽  
Author(s):  
Morio Saito ◽  
Akira Oh-Hashi ◽  
Mika Kubota ◽  
Eiichi Nishide ◽  
Michio Yamaguchi
Keyword(s):  
Soya Bean ◽  
Dietary Lipids ◽  
Mixed Function Oxidase ◽  
Oxidase System ◽  
Sardine Oil ◽  
Demethylase Activity ◽  
Mixed Function Oxidase System ◽  
Mixed Oil ◽  
Cytochrome P 450 ◽  
Microsomal Mixed Function Oxidase

The influence of dietary lipids on the liver microsomal mixed function oxidase system and on pentobarbital-induced sleeping time was studied in rats. Giving diets containing (g/kg) 150 olive oil, 150 lard or 150 soya-bean oil for 21 d (Expt 1) increased the cytochrome P-450 content in the order: olive oil < lard < soya-bean oil. When diets containing (g/kg) 150 lard, 150 soya-bean oil, 150 sardine oil or an equal mixture of 50 of each oil were given for 15 d (Expt 2), the cytochrome P-450 content and aminopyrine N-demethylase activity were significantly higher in the sardine-oil and mixed-oil groups than in the lard group, and the activity of aminopyrine N-demethylase was also significantly higher in the soya-bean oil group compared with the lard group. A significantly higher activity of NADPH-cytochrome c reductase (EC 1.6.2.5) was observed in the sardine-oil group than in the other three groups. Aniline hydroxylase activity and cytochrome b5 content remained unchanged in all the groups. Pentobarbital-induced sleeping time measured on day 15 (Expt 2) varied inversely with the changes in cytochrome P-450 content and aminopyrine N-demethylase activity in the three single-fat groups, but not in the mixed-oil group, reflecting liver microsomal metabolic activity for pentobarbital in vivo. From these results, it appears that high intakes of polyunsaturated fatty acids (18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3) stimulate the liver microsomal mixed function oxidase system.

Phenobarbitone-induced urinary excretions of D-glucaric acid and 6β-hydroxycortisol in man

1976 ◽  
Vol 54 (5) ◽  
pp. 778-782 ◽  
Author(s):  
A. N. Latham ◽  
P. Turner ◽  
C. Franklin ◽  
W. Maclay
Keyword(s):  
Normal Subjects ◽  
Mixed Function Oxidase ◽  
Glucaric Acid ◽  
Oxidase System ◽  
Mixed Function Oxidase System ◽  
Baseline Values ◽  
Microsomal Mixed Function Oxidase

The urinary excretions of D-glucaric acid and 6β-hydroxycortisol were determined in normal subjects before, during, and after 14 days treatment with placebo or phenobarbitone. The excretion of both metabolites was significantly potentiated by phenobarbitone and returned to baseline values 1 month after treatment was withdrawn. It was suggested that the determination of urinary D-glucaric acid reflects the activity of the hepatic microsomal mixed function oxidase system after the administration of an inducing agent such as phenobarbitone.

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