Intestinal absorption of free oleic acid in the unanesthetized rat: evidence for a saturable component?

1984 ◽  
Vol 62 (9) ◽  
pp. 1136-1140 ◽  
Author(s):  
Daniel Hollander ◽  
San Laung Chow ◽  
Violetta D. Dadufalza
Keyword(s):  
Oleic Acid ◽  
Intestinal Absorption ◽  
Sodium Taurocholate ◽  
Water Layer ◽  
Unstirred Layer ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Rate Of Absorption ◽  
Free Oleic Acid ◽  
Arachidonic Acids

The intestinal absorption of free oleic acid at low intraluminal concentrations and the influence of luminal factors on its absorption were studied in the unanesthetized rat. The relationship between oleic acid concentration (30–2500 μM) and its rate of absorptions fitted best to a rectangular hyperbola (y = x/(2.19 + 0.0015x), r = 0.94). Oleic acid's rate of absorption increased as the hydrogen ion and sodium taurocholate concentrations were increased or as the thickness and resistance of the unstirred water layer were diminished or following the addition of lysolecithin. The additions of the artificial detergent Tween-80, or lecithin and linoleic, linolenic and arachidonic acids to the perfusate decreased oleic acid's rate of absorption. It was concluded that oleic acid absorption in this range of concentrations displays apparent saturation kinetics which are due to unstirred layer effects, limited aqueous solubility of oleic acid and possible saturation of cytosol fatty acid binding proteins. Factors which increase oleic acid's protonated concentration or diminish the unstirred layer resistance, enhance its absorption rate, while factors which enhance its micellar solubility or interfere with its transfer out of the cell membrane decrease its overall rate of absorption.

scholarly journals Bioinformatics exploration of olive oil: molecular targets and properties of major bioactive constituents

OCL ◽  
2021 ◽  
Vol 28 ◽  
pp. 36
Author(s):  
Toluwase Hezekiah Fatoki ◽  
Cecilia O. Akintayo ◽  
Omodele Ibraheem
Keyword(s):  
Oleic Acid ◽  
Linoleic Acid ◽  
Olive Oil ◽  
Oleanolic Acid ◽  
Tyrosine Phosphatase ◽  
Fatty Acid Binding Proteins ◽  
Protein Tyrosine Phosphatase 1B ◽  
Bioactive Constituents ◽  
Fatty Acid Binding ◽  
Maslinic Acid

Olive oil possesses medicinal properties which include antimicrobial, antioxidant and anti-inflammatory, anti-diabetes, and anti-cardiovascular diseases. Oleic acid is the most abundant (95%) constituent of olive oil and others include linoleic acid, oleuropein, oleanolic acid, maslinic acid, melatonin, and others. The objective of this study is to predict the molecular targets and properties of key bioactive components of olive oil in human. Bioinformatics methods, which involved pharmacokinetics prediction, target prediction and gene network analyses, were used. The results showed that oleic acid has similar targets with linoleic acid, and showed significant probability of binding to several targets such as fatty acid-binding proteins in the adipose, epidermal, liver and muscle as well as alpha, delta and gamma peroxisome proliferator-activated receptors (PPARs). Carbonic anhydrase showed to be the only significant target of tyrosol, while protein-tyrosine phosphatase 1B, and CD81 antigen were targeted by maslinic acid and oleanolic acid. This study has applauded oleic acid, linoleic acid and tyrosol as olive oil bioactive constituents that have several potential pharmacological effects in humans that modulate several enzymes, receptors and transcription factors. The future work will be to investigate the effects of oleic acid on fatty acid-binding proteins and telomerase reverse transcriptase; melatonin on quinone reductase 2; tyrosol on carbonic anhydrase II; maslinic acid and oleanolic acid on protein-tyrosine phosphatase 1B.

Vitamin K1 intestinal absorption in vivo: influence of luminal contents on transport.

1977 ◽  
Vol 232 (1) ◽  
pp. E69 ◽  
Author(s):  
D Hollander ◽  
E Rim ◽  
K S Muralidhara
Keyword(s):  
Intestinal Absorption ◽  
Absorption Rate ◽  
Saturated Fatty Acids ◽  
Sodium Taurocholate ◽  
Water Layer ◽  
Vitamin K1 ◽  
Unstirred Water Layer ◽  
Saturation Kinetics ◽  
Nonionic Detergent

Intestinal absorption of [3H]phylloquinone was investigated in the unanesthetized rat by the use of a technique of recirculating perfused isolated intestinal segments. Apparent saturation kinetics were found as the concentration of the vitamin in the perfusate was increased in a stepwise fashion from 15 nM to 300 muM. Alkalinization of the perfusate or the addition of 2.5 mM linoleic acid to the perfusate caused a significant (P less than 0.05) decrease in the absorption rate of phylloquinone. Modifications in the perfusate concentration of sodium taurocholate, the substitution of a nonionic detergent (Pluronic F-68) for sodium taurocholate, the addition of medium- and long-chain saturated fatty acids, or the addition of vitamins K2 and K3 to the perfusate did not alter the absorption rate of the vitamin. Decreasing the thickness of the unstirred water layer by increasing the perfusion rate caused a significant increase in phylloquinone absorption rate. In vivo absorption of vitamin K1 appears to be mediated by an energy requiring saturable transport mechanism. The composition of the perfusate, its pH, and its rate of flow are all important determinants of vitamin K1 absorption rate.

Vitamin A1 intestinal absorption in vivo: influence of luminal factors on transport.

1977 ◽  
Vol 232 (5) ◽  
pp. E471 ◽  
Author(s):  
D Hollander ◽  
K S Muralidhara
Keyword(s):  
Intestinal Absorption ◽  
Absorption Rate ◽  
Sodium Taurocholate ◽  
Water Layer ◽  
Metabolic Inhibitors ◽  
Absorption Mechanism ◽  
Lymphatic Circulation ◽  
Passive Absorption

Intestinal absorption of [3H]retinol was studied in the unanesthetized rat. Luminal perfusate was recirculated through isolated intestinal segments with intact vascular and lymphatic circulation. Apparent saturation kinetics were found in physiological concentrations of retinol, whereas a linear relationship between the concentration and absorption rate was found at pharmacological concentrations of retinol in the perfusate. In physiological concentrations, retinol uptake in vitro by everted gut sacs was unaffected by anoxia or metabolic inhibitors and uncouplers. In vivo retinol absorption rate was decreased when sodium taurocholate concentration was raised above 5 mM, or when 2.5 mM linoleic or linolenic acids were added to the perfusate. Absorption increased markedly as the thickness of the unstirred water layer was diminished. Variations in perfusate pH from 4.5 to 8.6 did not change the retinol absorption rate. In vivo absorption of retinol in physiological concentrations is mediated by a saturable, carrier-mediated passive absorption mechanism modified by the presence of fatty acids of varying chain length.

Intestinal Absorption of Iodine131-Labeled Triolein and Oleic Acid in Normal Subjects and in Steatorrhea

1958 ◽  
Vol 34 (5) ◽  
pp. 901-909 ◽  
Author(s):  
Ervin Kaplan ◽  
Bernard D. Edidin ◽  
Robert C. Fruin ◽  
Lyle A. Baker
Keyword(s):  
Oleic Acid ◽  
Intestinal Absorption ◽  
Normal Subjects

Bionanomaterials: Thermal Stability of the Oleic Acid / alpha- and beta-cyclodextrin Complexes

2008 ◽  
Vol 59 (9) ◽  
Author(s):  
Daniel I. Hadaruga ◽  
Nicoleta G. Hadaruga ◽  
Anca Hermenean ◽  
Adrian Rivis ◽  
Vasile Paslaru ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Oleic Acid ◽  
Solution Method ◽  
Degradation Products ◽  
Water Solution ◽  
Fatty Acid Esters ◽  
Cyclodextrin Complexes ◽  
Free Oleic Acid ◽  
Thermal Stability Of ◽  
Acid Esters

This paper presents the thermal stability of the oleic acid encapsulated in a- and b - cyclodextrin. The complexation of the oleic acid was achieved by the ethanol-water solution method and the nanoparticles were analyzed by DSC. The free oleic acid and the encapsulated one were subjected to the thermal degradation in the range of 50-150�C and the degradation products were identified and quantified by GC-MS analysis of the fatty acid esters obtained by deriving with methanol/boron trifluoride, both for free compounds and for the encapsulated ones. The oleic acid complexes were very stable in this range of temperature.

scholarly journals Fatty acid transfer between multilamellar liposomes and fatty acid-binding proteins.

1984 ◽  
Vol 259 (21) ◽  
pp. 13395-13401 ◽  
Author(s):  
P Brecher ◽  
R Saouaf ◽  
J M Sugarman ◽  
D Eisenberg ◽  
K LaRosa
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Multilamellar Liposomes
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