Influence of adrenalectomy and sex on the binding of 3-methylcholanthrene to cytosol macromolecules of rat liver

1979 ◽  
Vol 57 (9) ◽  
pp. 1032-1036
Author(s):  
C. A. H. Bigger ◽  
C. R. Short
Keyword(s):  
Ion Exchange ◽  
Rat Liver ◽  
Column Chromatography ◽  
Cytosol Fraction ◽  
Exchange Column ◽  
Significant Difference ◽  
Ion Exchange Column

The retention of 3-methylcholanthrene (3-MC) in rat hepatic cytosol was significantly enhanced by adrenalectomy. In contrast, there was no significant difference in 3-MC retention in females as compared with males. 3-MC present in the cytosol fraction was bound to macromolecules and could be separated into three fractions by ion-exchange column chromatography.

scholarly journals The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers

1980 ◽  
Vol 186 (1) ◽  
pp. 235-242 ◽  
Author(s):  
M J Connor ◽  
J A Blair
Keyword(s):  
Ion Exchange ◽  
Rat Liver ◽  
Paper Chromatography ◽  
Column Chromatography ◽  
Lactobacillus Casei ◽  
Deae Cellulose ◽  
Microbiological Assay ◽  
Exchange Column ◽  
Chromatographic Techniques ◽  
Ion Exchange Column

About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labelled folate was incorporated into two folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, though less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0nmol/g) and 10-formylpteroyltetraglutamate (0.25nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase treatment.

Quantitative Method for Estimating Fecal Amino Acids

1975 ◽  
Vol 21 (10) ◽  
pp. 1437-1440 ◽  
Author(s):  
Christopher W I Owens ◽  
Walfredo Padovan
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Column Chromatography ◽  
Quantitative Method ◽  
Normal Values ◽  
Amino Nitrogen ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Fresh Stool

Abstract We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 µmol of α-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.

Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

2007 ◽  
Vol 70 (3) ◽  
pp. 493-498 ◽  
Author(s):  
Tsutomu Arakawa ◽  
Kouhei Tsumoto ◽  
Daisuke Ejima ◽  
Yoshiko Kita ◽  
Yasushi Yonezawa ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Ammonium Sulfate ◽  
Column Chromatography ◽  
Exchange Column ◽  
Ion Exchange Column

scholarly journals Fractionnement sur échangeur anionique des colloïdes glucidiques solubles (gommes et pectines) du jus de raisin sain

OENO One ◽  
1980 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Denis Dubourdieu ◽  
Pascal Ribéreau-Gayon
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Galacturonic Acid ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Neutral Sugars

<p style="text-align: justify;">Les gommes d'un moût de raisins sains sont fractionnés par chromatographie d'échange anionique sur D.E.A.E. Sephadex A 25. On peut ainsi caractériser des gommes neutres qui ne contiennent que des oses neutres dans leur molécule et des gommes acides qui comportent des oses neutres et de l'acide galacturonique.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Gums of juice from healthy grappes are fractionnated by ion exchange column chromatography on D.E.A.E. Sephadex A 25. So are separated neutral gums, containing only neutral sugars in their molecules, from acidic gums, that contains neutral sugars and galacturonic acid.</p>

Phosphonodipeptides: synthesis and separation of diastereoisomers

1982 ◽  
Vol 60 (24) ◽  
pp. 3081-3084 ◽  
Author(s):  
Paweł Kafarski ◽  
Barbara Lejczak ◽  
Przemsław Mastalerz ◽  
Jerzy Szewczyk ◽  
Czesław Wasielewski
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Exchange Column ◽  
Ion Exchange Column

Diastereoisomeric mixtures of phosphonodipeptides, prepared from racemic dialkyl 1-aminoalkanephosphonates, were separated by ion-exchange column chromatography. Phosphonodipeptides were found to be much more readily available from dialkyl 1-aminoalkanephosphonates than from the corresponding free acids and the mixed carboxylic – carbonic anhydride method was found to be the most effective one.

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