scholarly journals The identification of the folate conjugates found in rat liver 48 h after the administration of radioactively labelled folate tracers

1980 ◽  
Vol 186 (1) ◽  
pp. 235-242 ◽  
Author(s):  
M J Connor ◽  
J A Blair
Keyword(s):  
Ion Exchange ◽  
Rat Liver ◽  
Paper Chromatography ◽  
Column Chromatography ◽  
Lactobacillus Casei ◽  
Deae Cellulose ◽  
Microbiological Assay ◽  
Exchange Column ◽  
Chromatographic Techniques ◽  
Ion Exchange Column

About 70% of the radioactivity retained in the livers of rats dosed 48 h earlier with radioactively labelled folate was incorporated into two folate conjugates. The major derivative was purified and isolated by Sephadex G-15, DEAE-cellulose and DEAE-Sephadex ion-exchange column chromatography and paper chromatography. It was identified as 10-formylpteroylpentaglutamate by a combination of spectral, microbiological, chemical and chromatographic techniques. The minor conjugate, though less well characterized, exhibited similar properties and was assigned the structure 10-formylpteroyltetraglutamate. 10-Formylpteroylpentaglutamate (2.0nmol/g) and 10-formylpteroyltetraglutamate (0.25nmol/g) comprised about 20% of the total endogenous hepatic folate as determined by microbiological assay (Lactobacillus casei after conjugase treatment.

Influence of adrenalectomy and sex on the binding of 3-methylcholanthrene to cytosol macromolecules of rat liver

1979 ◽  
Vol 57 (9) ◽  
pp. 1032-1036
Author(s):  
C. A. H. Bigger ◽  
C. R. Short
Keyword(s):  
Ion Exchange ◽  
Rat Liver ◽  
Column Chromatography ◽  
Cytosol Fraction ◽  
Exchange Column ◽  
Significant Difference ◽  
Ion Exchange Column

The retention of 3-methylcholanthrene (3-MC) in rat hepatic cytosol was significantly enhanced by adrenalectomy. In contrast, there was no significant difference in 3-MC retention in females as compared with males. 3-MC present in the cytosol fraction was bound to macromolecules and could be separated into three fractions by ion-exchange column chromatography.

Quantitative Method for Estimating Fecal Amino Acids

1975 ◽  
Vol 21 (10) ◽  
pp. 1437-1440 ◽  
Author(s):  
Christopher W I Owens ◽  
Walfredo Padovan
Keyword(s):  
Amino Acids ◽  
Ion Exchange ◽  
Column Chromatography ◽  
Quantitative Method ◽  
Normal Values ◽  
Amino Nitrogen ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Fresh Stool

Abstract We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 µmol of α-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.

Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

2007 ◽  
Vol 70 (3) ◽  
pp. 493-498 ◽  
Author(s):  
Tsutomu Arakawa ◽  
Kouhei Tsumoto ◽  
Daisuke Ejima ◽  
Yoshiko Kita ◽  
Yasushi Yonezawa ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Ammonium Sulfate ◽  
Column Chromatography ◽  
Exchange Column ◽  
Ion Exchange Column

Composition of Pteroylpolyglutamates (Conjugated Folates) in Guinea-Pig Liver and Their Formation from Folic Acid

1972 ◽  
Vol 43 (6) ◽  
pp. 799-813 ◽  
Author(s):  
R. Corrocher ◽  
B. K. Bhuyan ◽  
A. V. Hoffbrand
Keyword(s):  
Folic Acid ◽  
Guinea Pig ◽  
Lactobacillus Casei ◽  
Deae Cellulose ◽  
Microbiological Assay ◽  
Pig Liver ◽  
Ion Exchange Column ◽  
Pteroylglutamic Acid ◽  
Guinea Pig Liver ◽  
Liver Folate

1. The composition of guinea-pig liver folates and the biochemical route of formation of liver folates from injected tritium-labelled pteroylglutamic acid (folic acid) have been studied. 2. Endogenous folate was measured by microbiological assay with Lactobacillus casei and Streptococcus faecalis, with and without deconjugation of whole liver pteroylpolyglutamates (conjugated folates). Individual folate compounds were identified by microbiological assay after fractionation of liver folates by DEAE cellulose ion-exchange column chromatography. 3. Liver folate in the guinea-pig consists of about 84–87% reduced pteroylpolyglutamates with more than three glutamate moieties/molecule, about 12–15% reduced pteroyltriglutamates, about 1% reduced pteroyldiglutamates and only traces of reduced pteroylmonoglutamates. 4. About 53% of the liver folate consists of methylated derivatives. 5. Injected pteroylglutamic acid was first rapidly reduced and formylated or methylated. Glutamate moieties were then added, probably singly, to form di-, tri- and poly-glutamates. This was a relatively slow process with a hold-up at the triglutamate stage. 6. The proportion of the labelled pteroylglutamic acid in the polyglutamate form approximated to the proportion of endogenous folates in this form after 3–4 days. 7. The amount of radioactive folate in the liver increased progressively from 1 to 84 h after injection of a standard amount of radioactive pteroylglutamic acid.

scholarly journals Fractionnement sur échangeur anionique des colloïdes glucidiques solubles (gommes et pectines) du jus de raisin sain

OENO One ◽  
1980 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Denis Dubourdieu ◽  
Pascal Ribéreau-Gayon
Keyword(s):  
Ion Exchange ◽  
Column Chromatography ◽  
Galacturonic Acid ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Neutral Sugars

<p style="text-align: justify;">Les gommes d'un moût de raisins sains sont fractionnés par chromatographie d'échange anionique sur D.E.A.E. Sephadex A 25. On peut ainsi caractériser des gommes neutres qui ne contiennent que des oses neutres dans leur molécule et des gommes acides qui comportent des oses neutres et de l'acide galacturonique.</p><p style="text-align: justify;">+++</p><p style="text-align: justify;">Gums of juice from healthy grappes are fractionnated by ion exchange column chromatography on D.E.A.E. Sephadex A 25. So are separated neutral gums, containing only neutral sugars in their molecules, from acidic gums, that contains neutral sugars and galacturonic acid.</p>

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