Alteration of the erythrocyte ultrastructure and blood viscosity by morphine

1978 ◽  
Vol 56 (2) ◽  
pp. 245-251 ◽  
Author(s):  
E. B. Vadas ◽  
E. A. Hosein
Keyword(s):  
Cell Volume ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Plasma Viscosity ◽  
Cell Geometry ◽  
Flow Properties ◽  
Mean Cell Volume ◽  
Morphine Administration ◽  
The Mean ◽  
Volume Cell

The effects of acute morphine administration on intact erythrocytes and on their flow properties were studied by measuring the mean cell volume, cell geometry, and whole blood and plasma viscosities. Morphine caused a small (2–7%) increase in mean cell volume. Changes in cell geometry were found to be time dependent and most pronounced in concave portions of the red cells. Whole blood viscosity was found to decrease upon morphine treatment; this may be due in part to a concurrent decrease in plasma viscosity.

Viscosity and Red Cell Deformity in Arterial Disease

1979 ◽  
Author(s):  
G Cella ◽  
H de Haas ◽  
M Rampling ◽  
V Kakkar
Keyword(s):  
Vascular Disease ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Arterial Disease ◽  
Plasma Viscosity ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Red Cell ◽  
Whole Blood Viscosity ◽  
Significant Difference

Haemorrheological factors have been shown to be affected in many kings of vascular disease. The present study was undertaken to correlate these factors in normal subjects and patients suffering from peripheral arterial disease. Twenty-two patients were investigated; they had moderate or severe intermittent claudication, extent of disease being confirmed by aorto-arteriography and ankle-systolic pressure studies. Twenty-five controls with no symptoms or signs of arterial disease were selected with comparable age and sex distribution. Whole blood viscosity was measured at shear rates of 230 secs-1 and 23 secs-lat 37°c using a Wells Brookfield cone plate microvisco meter. Plasma viscosity was also measured in an identical manner. Erythrocyte flexibility was measured by centrifuge technique and fibrinogen concentration as well as haematocrit by standard techniques. The fibrinogen concentration appeared to be the only significant parameter; the mean concentration in patients with peripheral vascular disease of 463 ± 73mg/l00ml in the control group ( < 0.05). Although whole blood viscosity was high in patients, when corrected to a common haematocrit, there was no significant difference between patients and controls. The same megative correlation was found for plasma viscosity. The red cell flexibility was found to be increased in patients as compared to the control group, but this effect appeared to be simply proportional to the fibrinogen concentration.

scholarly journals The mean cell volume difference (dMCV) reflects serum hypertonicity in diabetic dogs

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219864 ◽  
Author(s):  
Olga C. Norris ◽  
Thomas Schermerhorn
Keyword(s):  
Cell Volume ◽  
Mean Cell Volume ◽  
The Mean ◽  
Diabetic Dogs ◽  
Volume Difference

scholarly journals Cell size and plamitate-1-14c turnover of rabbit marrow fat

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 599-605 ◽  
Author(s):  
S Trubowitz ◽  
A Bathija
Keyword(s):  
Fatty Acids ◽  
Cell Volume ◽  
Unsaturated Fatty Acids ◽  
Fat Cell ◽  
Marrow Fat ◽  
Mean Cell Volume ◽  
Triglyceride Fraction ◽  
Mean Diameter ◽  
The Mean ◽  
Perinephric Fat

Abstract Adipose tissue is a major component of normal rabbit marrow. Morphological considerations suggest an active role for this tissue in hematopoiesis. This hypothesis was tested by injecting 50 micronCi of palmitate-1–14C intravenously into fed, hematologically normal New Zealand rabbits. The animals were sacrificed 24 hr later and the femoral marrow removed. Samples of subcutaneous and perinephric fat were taken for comparison. The fat cells were isolated by the Rodbell method and the diameters measured. Incorporation of the 14C-palmitate in the triglyceride fraction was determined and the composition of the fatty acids was measured by gas chromatography. The mean diameter of the marrow fat cell was 46 micronm (mean cell volume 55 pl); the mean diameter of the perinephric fat cell 70 micronm (mean cell volume 200 pl). 14C-Palmitate turnover per gram triglyceride was some fivefold greater in the marrow fat; however, when expressed on a cell basis, the turnover for the marrow and perinephric fat cell was similar. The marrow fat contained a higher concentration of unsaturated fatty acids. These findings suggest that there is greater lipolysis and lesser storage in the marrow fat than in the perinephric.

scholarly journals Reference and cut-off values for serum ferritin, mean cell volume, and hemoglobin to diagnose iron deficiency in infants aged 9 to 12 months

Medicina ◽  
2007 ◽  
Vol 43 (9) ◽  
pp. 698 ◽  
Author(s):  
Neve Vendt ◽  
Tiina Talvik ◽  
Pille Kool ◽  
Sirje Leedo ◽  
Karel Tomberg ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Cell Volume ◽  
Serum Ferritin ◽  
Reference Values ◽  
Mean Cell Volume ◽  
Receiver Operating Characteristic Curves ◽  
Healthy Infants ◽  
Diagnostic Characteristics ◽  
The Mean ◽  
Better Than

Background. The number of different laboratory tests and reference values are used to diagnose iron deficiency, but there is no agreement regarding the diagnostic criteria for infants. Aim of study. To establish reference values for serum ferritin, mean cell volume, and hemoglobin in infants aged from 9 to 12 months in Estonia and to evaluate the diagnostic characteristics of serum ferritin, mean cell volume, and hemoglobin in the diagnosis of iron deficiency. Methods. Altogether 195 healthy infants aged 9–12 months participated in the study. They were randomly selected out of 300 families from seven different counties from all over Estonia. Serum ferritin, hemoglobin, soluble transferrin receptor (sTfR) levels and mean cell volume were measured. The best cut-off values for serum ferritin, mean cell volume, and hemoglobin to diagnose iron deficiency, defined by sTfR>2.45 mg/L (n=25), were determined by receiver operating characteristic curves. Results. The mean and reference values (5th and 95th centiles) for ferritin was 24 µg/L (4– 55), 73 fl (68–80) for mean cell volume, and 112 g/L (101–128) for hemoglobin. The best cut-off values to diagnose iron deficiency were <10.9 µg/L for serum ferritin (sensitivity of 83% and specificity of 80%), <71 fl for mean cell volume (86% and 83%, respectively), and <107 g/L for hemoglobin (67% and 87%, respectively). The sensitivity and specificity of serum ferritin and mean cell volume in the diagnosis of iron deficiency were better than those of hemoglobin. Conclusion. For the diagnosis of iron deficiency in infants aged 9–12 months, the cut-off values of <10.9 µg/L and <71 fl should be used for serum ferritin and mean cell volume, respectively.

An assessment of the incidence of iron deficiency in paediatric otolaryngology inpatients

1991 ◽  
Vol 105 (12) ◽  
pp. 1021-1024 ◽  
Author(s):  
Judith M. Heaton ◽  
Robin L. Blair ◽  
Clemency Shadbolt ◽  
Helen Christmas
Keyword(s):  
Regression Analysis ◽  
Iron Deficiency ◽  
Haemoglobin Level ◽  
Cell Volume ◽  
Surgical Procedures ◽  
Ferritin Level ◽  
Preoperative Screening ◽  
Mean Cell Volume ◽  
Economic Class ◽  
The Mean

AbstractThe aims of this study were: to determine whether there is an increased incidence of iron deficiency in paediatric otolaryngology inpatients compared with other surgical controls; and to establish whether preoperative screening of haemoglobin level is warranted in such patients.Children aged 1–10 years admitted electively for ENT surgery or for general surgical procedures had blood taken for haemoglobin level, mean cell volume and serum ferritin. Their age, weight, socio-economic class and ethnic background were recorded.A total of 100 patients entered the study, in a six-month period. The mean ages and weights for the two groups were statistically different, so allowance was made for this in calculations. Social class was not significantly different. No relationship could be established between haemoglobin level and ferritin level for individual patients. Multiple regression analysis for haemoglobin level, mean cell volume and for ferritin level showed that allowing for the age and weight differences these variables were not significantly different for the two groups.This study has therefore shown no increased incidence of iron deficiency in paediatric ENT inpatients. Each Department should formulate its own policy on pre-operative haemoglobin screening, based on local considerations.

Blood Viscosity, Glycaemic Control and Retinopathy in Insulin-Dependent Diabetes

1982 ◽  
Vol 63 (2) ◽  
pp. 211-216 ◽  
Author(s):  
P. Y. W. Poon ◽  
T. L. Dornan ◽  
C. Orde-Peckar ◽  
R. Mullins ◽  
A. J. Bron ◽  
...  
Keyword(s):  
Cell Volume ◽  
Blood Viscosity ◽  
Packed Cell Volume ◽  
Cross Sectional Study ◽  
Plasma Viscosity ◽  
Glycosylated Haemoglobin ◽  
Trend Test ◽  
Diabetic Patients ◽  
Cross Sectional ◽  
Low Shear

1. In a cross-sectional study, 32 insulin-treated diabetic patients had elevated low shear (27·1 vs 22·1 mPa s, P < 0·05) and high shear blood viscosity (5·4 vs 5·0 mPa s, P < 0·05) when compared with 10 non-diabetic controls. After correction to 45% packed cell volume, the abnormality had a tendency to be greater in patients with proliferative (mean low shear viscosity, 30·8 mPa s) than background (29·2 mPa s) or nil/minimal retinopathy (27·6 mPa s, 0·05 < P < 0·07, permutational trend test). 2. The fibrinogen levels were higher in the diabetic group (P < 0·05) and correlated with the low shear blood viscosity (rs = 0·38, P < 0·05). 3. In a prospective study, 74 insulin-treated diabetic patients with background retinopathy were randomized into two groups. Thirty-six patients were on attempted improved therapy (A group); in these the mean glycosylated haemoglobin (Hb A1c) fell within 1 year (11·6 to 10·1%, P < 0·001). Both the corrected low shear blood and plasma viscosity fell similarly (P < 0·001). The fall was greater than in those patients who were kept on usual therapy (U group) and whose glycosylated haemoglobin did not change significantly (11·7 to 11·4%) over the year. 4. The effect of diabetes on blood viscosity may not be a direct pathological factor, as the same increased viscosity would be produced by a mean increase of 1·7% in packed cell volume, compared with a population range of 14% packed cell volume.

Viscosity and Red Cell Deformity in Arterial Disease

1979 ◽  
Author(s):  
G. Cella ◽  
H.A. de Haas ◽  
M. Rampling ◽  
V.V. Kakkar
Keyword(s):  
Vascular Disease ◽  
Blood Viscosity ◽  
Whole Blood ◽  
Arterial Disease ◽  
Plasma Viscosity ◽  
Control Group ◽  
Fibrinogen Concentration ◽  
Red Cell ◽  
Whole Blood Viscosity ◽  
Significant Difference

Haemorrheological factors have been shown to be affected in many kings of vascular disease. The present study was undertaken to correlate these factors in normal subjects and patients suffering from peripheral arterial disease. Twenty-two patients were investigated; they had moderate or severe intermittent claudication, extent of disease being confirmed by aorto-arteriography and ankle-systolic pressure studies. Twenty-five controls with no symptoms or signs of arterial disease were selected withcomparable age and sex distribution. Whole blood viscosity was measured at shear rates of 230 secs-1 and 23 sees-1 at 37°C using a Weils Brookfield cone plate microvisco meter. Plasma viscosity was also measured in an identical manner. Erythrocyte flexibility was measured by centrifuge technique and fibrinogen concentration as well as haematocrit by standard techniques. The fibrinogen concentration appeared to be the only significant parameter; the mean concentration in patients with peripheral vascular disease of 463 ± 73mg/100ml in the control group ( 〈 0.05). Although whole blood viscosity was high in patients, when corrected t. a common haematocrit, there was no significant difference between patients and controls The same megative correlation was found for plasma viscosity. The red cell flexibility was found to be increased in patients as compared to the control group, but this effect appeared to be simply proportional to the fibrinogen concentration.

Effects of Chloramphenicol on Growth, Size Distribution, Chlorophyll Synthesis and Ultrastructure of Euglena Gracilis

1969 ◽  
Vol 4 (3) ◽  
pp. 627-644
Author(s):  
Y. BEN-SHAUL ◽  
Y. MARKUS
Keyword(s):  
Protein Synthesis ◽  
Cell Division ◽  
Cell Volume ◽  
Chlorophyll Synthesis ◽  
Secondary Effect ◽  
Mean Cell Volume ◽  
Initial Rise ◽  
Dividing Cells ◽  
Light Inhibition ◽  
The Mean

Multiplication of Euglena cells treated by 0.5-1.0 mg/ml chloramphenicol was not disturbed for the first 36 h and inhibition appeared only at later stages. The mean cell volume of treated dividing cells was decreased, although the initial rise in cell volume, which normally occurred during the first 12 h of incubation, was not prevented. The antibiotic also lowered the chlorophyll content of green dividing cells. In dard-grown cells transferred to light, inhibition of chlorophyll synthesis was immediate but not complete, and was followed by a decreased rate of plastid elongation and thylakoid formation. Our findings suggest that chloramphenicol does not cause the loss of existing pigment and that impaired chlorophyll synthesis is a secondary effect of inhibition of protein synthesis. The results also indicate that the greening process is more sensitive than cell division to the antibiotic.

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