Myocardial lesions in rats fed rapeseed oil. II. Effects of castration

1977 ◽  
Vol 55 (2) ◽  
pp. 265-271 ◽  
Author(s):  
H. W. Hulan ◽  
J. K. G. Kramer ◽  
A. H. Corner ◽  
B. Thompson
Keyword(s):  
Rapeseed Oil ◽  
Corn Oil ◽  
Sprague Dawley ◽  
Semisynthetic Diet ◽  
Sprague Dawley Rats ◽  
Body Weights ◽  
Two Factors ◽  
The Mean ◽  
Heart Lesions ◽  
Myocardial Lesions

The effects of castration on the incidence of myocardial lesions in rats were investigated in an experiment which included two factors: sex (entire males, castrated males, entire females and castrated (ovariohysterectomized) females) and diets (5% corn oil, 20% corn oil, and 20% Brassica napus var. Zephyr rapeseed oil). For 16 weeks, each of the 12 groups of 30 Sprague–Dawley rats, housed 2 per cage, were fed ad libitum the test oils incorporated in a semisynthetic diet. At each weighing, the mean body weights for each diet were highest in entire males followed by castrated males, castrated females, and entire females with all differences significant (P < 0.05). The results indicated that castration did not influence cardiac fatty acid composition. The incidence of myocardial lesions in entire and castrated females and in castrated males was similar while significantly more entire males developed lesions (P < 0.001). Rats fed a diet containing 20% Zephyr rapeseed oil showed a significantly (P < 0.001) higher incidence of heart lesions than did rats fed diets containing 5% or 20% corn oil. Similarly, significantly (P < 0.05) more rats fed the 20% corn oil diet had lesions than rats fed the 5% corn oil diet. The involvement of androgens in the formation of myocardial lesions is suggested, since castration significantly lowered the incidence in males but not in females.

Myocardial lesions in rats fed rapeseed oil. I. Influence of strain of rats

1977 ◽  
Vol 55 (2) ◽  
pp. 258-264 ◽  
Author(s):  
H. W. Hulan ◽  
J. K. G. Kramer ◽  
A. H. Corner
Keyword(s):  
Fatty Acid Composition ◽  
Rapeseed Oil ◽  
Corn Oil ◽  
The Other ◽  
Sprague Dawley ◽  
High Incidence ◽  
Two Factors ◽  
Ad Libitum ◽  
Different Strains ◽  
Myocardial Lesions

The influence of strain of rat on the development of myocardial lesions was investigated in an experiment which included two factors: strain (Wistar, Sherman, Chester Beatty (Hooded), and Sprague–Dawley from two sources designated Sprague–Dawley A, Sprague–Dawley C) and diet (5% corn oil, 20% corn oil, and 20% Brassica napus var. Zephyr rapeseed oil). Groups of 30 rats, housed 2 per cage, from each of three different strains of rats and groups of 10 rats, housed 2 per cage, from one strain of rats (Hooded) were fed semisynthetic diets containing the test oils for 16 weeks on an ad libitum basis. Rats of the Hooded strain consumed considerably less feed and grew at a significantly (P < 0.01) slower rate than did rats of the Sprague–Dawley C strain, which in turn ate less and grew significantly (P < 0.01) slower than rats of the other three strains. No marked differences were observed in the fatty acid composition of total cardiac lipids among strains. Rats from all strains except Hooded fed the diet containing 20% Zephyr RSO had a significantly (P < 0.001) higher incidence of myocardial lesions than rats fed the 20% corn oil diet which in turn had a significantly (P < 0.001) higher incidence than rats fed 5% corn oil. Similarly, significantly (P < 0.05) more Sprague–Dawley C rats had myocardial lesions than Sprague–Dawley A rats regardless of diet. None of the Hooded rats fed the diet containing 20% Zephyr RSO developed myocardial lesions whereas all other strains fed this diet developed a high incidence of myocardial lesions.

The development of dermal lesions and alopecia in male rats fed rapeseed oil

1976 ◽  
Vol 54 (1) ◽  
pp. 1-6 ◽  
Author(s):  
H. W. Hulan ◽  
W. G. Hunsaker ◽  
J. K. G. Kramer ◽  
S. Mahadevan
Keyword(s):  
Fatty Acid ◽  
Rapeseed Oil ◽  
Corn Oil ◽  
Fatty Acid Analysis ◽  
Male Rats ◽  
Sprague Dawley ◽  
Semisynthetic Diet ◽  
Prostaglandin Biosynthesis ◽  
Sprague Dawley Rats ◽  
Dermal Lesions

For 8 weeks 10 male weanling Sprague–Dawley rats were fed a semisynthetic diet containing by weight either 20% corn oil or rapeseed oils containing different amounts of erucic acid (Brassica napus var. Zephyr, 0.6%; B. napus var. Oro, 1.8%; B. campestris var. Span, 4.8%; or B. campestris var. Echo and Arlo, i.e., regular rapeseed oil, 23.6%). At 4–5 weeks after the experiment began, rats receiving the diets containing rapeseed oil showed evidence of alopecia and developed scaly, hemorrhagic, and necrotic tails, as well as scaliness of the feet, similar to the lesions described in essential fatty acid (EFA) deficiency. This condition became most severe between 5 and 8 weeks and had disappeared by 14 weeks. Fatty acid analysis of the diets and tissues of the animals did not reveal any evidence of EFA deficiency. It is suggested that these symptoms observed might be related to a possible inhibition of prostaglandin biosynthesis in rats fed rapeseed oils.

Electrocardiograms of male rats fed rapeseed oil

1977 ◽  
Vol 55 (5) ◽  
pp. 1116-1121 ◽  
Author(s):  
W. G. Hunsaker ◽  
H. W. Hulan ◽  
J. K. G. Kramer ◽  
A. H. Corner
Keyword(s):  
Rapeseed Oil ◽  
Corn Oil ◽  
Frontal Plane ◽  
P Wave ◽  
Male Rats ◽  
Qrs Complex ◽  
Sprague Dawley ◽  
Cardiac Rate ◽  
Sprague Dawley Rats ◽  
Wave Forms

Frontal plane electrocardiograms (standard limb leads) were recorded on male Sprague–Dawley rats fed for 14 weeks on semisynthetic diets containing 20% (by weight) of Brassica napus cv. Tower or B. campestris cv. Span rapeseed oil or fractions isolated from Span rapeseed oil. Control diets were rat Chow or semisynthetic diets containing 20% (by weight) of either safflower oil or corn oil. The amplitude of the P, Q, R, S, and T waves, the duration of the P wave, QRS complex, and P–R interval, and the cardiac rate were measured in the electrocardiograms and differences between groups compared statistically. While there were statistically significant differences in the amplitude of some of the wave forms, there were no consistent differences which could be attributed to the feeding of rapeseed oil or its fractions. The duration of the P wave and the QRS complex were significantly longer in some of the control groups than those in most of the treated groups. There was no correlation between the presence of a Q wave and the incidence of myocardial lesions in any group. Cardiac rate was essentially the same in all groups.

Evaluation of the Developmental Toxicity of α-Methyl-3,4-Methylene-Dioxyhydrocinnamic Aldehyde in Rats

2006 ◽  
Vol 25 (2) ◽  
pp. 127-132 ◽  
Author(s):  
Anne Marie Api ◽  
Elise M. Lewis ◽  
Alan M. Hoberman ◽  
Mildred S. Christian ◽  
Robert M. Diener
Keyword(s):  
Corn Oil ◽  
Developmental Toxicity ◽  
Clinical Signs ◽  
Feed Consumption ◽  
Corpora Lutea ◽  
Sprague Dawley ◽  
Pregnant Rats ◽  
Excessive Salivation ◽  
Sprague Dawley Rats ◽  
Body Weights

The developmental toxicity of α-methyl-3,4-methylene-dioxyhydrocinnamic aldehyde (MMDHCA), a widely used fragrance ingredient, was evaluated for developmental toxicity in Sprague-Dawley rats (25/group; cesarean-sectioning identified 21 to 25 pregnant rats/group). Oral dosages of 0 (corn oil), 62, 125, or 250 mg/kg/day were administered by gavage on days 7 through 17 of gestation (GDs 7 through 17). Rats were observed for viability, clinical signs, body weights, and feed consumption. Necropsy and cesarean sectioning occurred on GD 21. Uteri were examined for number and distribution of implantations, live and dead fetuses, and early and late resorptions. Numbers of corpora lutea were also recorded. Fetuses were weighed and examined for gender, gross external changes, and soft tissue or skeletal alterations. Analysis of dosage preparations verified calculated dosages ±10%. No deaths occurred. Excessive salivation occurred in all groups, but the incidence was increased at 250 mg/kg/day. The 250 mg/kg/day dosage also was associated with a significant increase in the incidences of a clear, red or yellow perioral and/or red perivaginal substance and significant reductions in mean feed consumption and body weight gains (11.6% and 7.4%, respectively) during the entire dosage period. No gross changes attributable to MMDHCA were observed at necropsy. Cesarean section or litter parameters, as well as fetal alterations, were not affected by MMDHCA at 250 mg/kg/day or either of the lower dosages tested. Based on these data, maternal and developmental no-observable-effect levels (NOAELs) of 125 and >250 mg/kg/day, respectively, were established for MMDHCA. It is concluded that MMDHCA is not a developmental toxicant in rats under the conditions of this study and dosing regimen.

RESPONSES OF TWO STRAINS OF RATS TO RAPESEED OIL AND CORN OIL

1960 ◽  
Vol 38 (3) ◽  
pp. 187-192 ◽  
Author(s):  
Joyce L. Beare ◽  
T. K. Murray ◽  
J. A. Campbell
Keyword(s):  
Vitamin A ◽  
Rapeseed Oil ◽  
Wistar Rats ◽  
Corn Oil ◽  
Strain Differences ◽  
Basal Diet ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Dietary Oil

Wistar and Sprague–Dawley rats were compared in their response to 20% rapeseed oil or corn oil in a basal diet of ground fox cubes. Apparent coefficients of digestibility were significantly lower for rapeseed oil than for corn oil in the Sprague–Dawley, but not in the Wistar rats. After 6 weeks the adrenals of Sprague–Dawley rats were larger in the animals fed rapeseed oil than in those fed corn oil, while the adrenals of Wistar rats were not influenced by the dietary oil. These strain differences explain some discrepancies appearing in the literature concerning the effects of rapeseed oil in the rat.In another experiment, Wistar and Sprague–Dawley rats deprived of vitamin A for 18 days were fed a purified basal diet with 20% rapeseed oil or corn oil and dosed with 75 or 150 I.U. of vitamin A per day. After 14 days there was no significant effect of the strain of rat or the type of dietary oil on the liver storage of vitamin A. The rate of depletion of vitamin A from the liver following a single dose of 1200 I.U. of vitamin A was also studied and indicated no significant difference attributable to the dietary oils.

scholarly journals Evaluation of the Developmental Toxicity of Alpha-iso-methylionone in Rats

2007 ◽  
Vol 26 (3) ◽  
pp. 271-276 ◽  
Author(s):  
Valerie T. Politano ◽  
Elise M. Lewis ◽  
Alan M. Hoberman ◽  
Mildred S. Christian ◽  
Robert M. Diener ◽  
...  
Keyword(s):  
Corn Oil ◽  
Developmental Toxicity ◽  
Clinical Signs ◽  
Feed Consumption ◽  
Corpora Lutea ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Body Weights ◽  
Tissue Changes

Alpha-iso-methylionone, a widely used fragrance ingredient, was evaluated for developmental toxicity in presumed pregnant Sprague-Dawley rats (25/group). Oral dosages of 0, 3, 10, or 30 mg/kg/day alpha-iso-methylionone in corn oil were administered by gavage on gestational days 7 to 17. The presence of spermatozoa and/or a copulatory plug in situ was designated as gestational day. Rats were observed for viability, clinical signs, body weights, and feed consumption. Caesarean sectioning and necropsy occurred on gestational day 21. Uteri were examined for number and distribution of implantations, live and dead fetuses, and early and late resorptions. Numbers of corpora lutea were also recorded. Fetuses were weighed and examined for gender, gross external changes, and soft tissue or skeletal alterations. No maternal or fetal deaths occurred. No fragrance ingredient–related clinical signs were observed. Feed consumption, body weight gains, gross tissue changes at necropsy, and caesarean section or litter parameters, as well as fetal developmental morphology, were unaffected by dosages of alpha-iso-methylionone as high as 30 mg/kg/day. Based on these data, maternal and developmental no observed adverse effect levels of equal to or greater than 30 mg/kg/day were established for alpha-iso-methylionone. It is concluded that alpha-iso-methylionone is not a developmental toxicant in rats at maternal doses of up to 30 mg/kg/day.

RESPONSES OF TWO STRAINS OF RATS TO RAPESEED OIL AND CORN OIL

1960 ◽  
Vol 38 (1) ◽  
pp. 187-192 ◽  
Author(s):  
Joyce L. Beare ◽  
T. K. Murray ◽  
J. A. Campbell
Keyword(s):  
Vitamin A ◽  
Rapeseed Oil ◽  
Wistar Rats ◽  
Corn Oil ◽  
Strain Differences ◽  
Basal Diet ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Dietary Oil

Wistar and Sprague–Dawley rats were compared in their response to 20% rapeseed oil or corn oil in a basal diet of ground fox cubes. Apparent coefficients of digestibility were significantly lower for rapeseed oil than for corn oil in the Sprague–Dawley, but not in the Wistar rats. After 6 weeks the adrenals of Sprague–Dawley rats were larger in the animals fed rapeseed oil than in those fed corn oil, while the adrenals of Wistar rats were not influenced by the dietary oil. These strain differences explain some discrepancies appearing in the literature concerning the effects of rapeseed oil in the rat.In another experiment, Wistar and Sprague–Dawley rats deprived of vitamin A for 18 days were fed a purified basal diet with 20% rapeseed oil or corn oil and dosed with 75 or 150 I.U. of vitamin A per day. After 14 days there was no significant effect of the strain of rat or the type of dietary oil on the liver storage of vitamin A. The rate of depletion of vitamin A from the liver following a single dose of 1200 I.U. of vitamin A was also studied and indicated no significant difference attributable to the dietary oils.

Ultrastructural Effects of Acute Kepone Treatment in Rats

1976 ◽  
Vol 34 ◽  
pp. 286-287
Author(s):  
Richard L. Klein ◽  
Åsa K. Thureson-Klein ◽  
Harihara M. Mehendale
Keyword(s):  
Neurological Disorders ◽  
Corn Oil ◽  
Preliminary Investigation ◽  
Reproductive Capacity ◽  
Ultrastructural Changes ◽  
Lipid Deposition ◽  
Severe Toxicity ◽  
Sprague Dawley ◽  
Ether Anesthesia ◽  
Sprague Dawley Rats

KeponeR (decachlorooctahydro-1,3,4-metheno-2H-cyclobuta[cd]pentalen-2-one) is an insecticide effective against ants and roaches. It can cause severe toxicity in fishes, birds, rodents and man. Prominent effects include hepatic lipid deposition and hypertrophy, impairment of reproductive capacity and neurological disorders. Mitochondrial oligomycin-sensitive Mg2+-ATPase is also inhibited. The present study is a preliminary investigation of tissue ultrastructural changes accompanying physiological signs of acute toxicity, which after two days treatment include: pronounced hypersensitivity and tremor, various degrees of anorexia and adipsia, and decreased weight gain.Three different series of adult male Sprague-Dawley rats (Charles River or CD-I) were treated by intubation with Kepone in corn oil at a dose of 50 mg per kg for 3 successive days or at 200 ppm in food for 8 days. After ether anesthesia, rats were immediately perfused via a cannula in the left ventricle with 4% p-formaldehyde and 0.5% glutaraldehyde in Millonig's phosphate buffer at pH 7.2 for 20-30 min at 22°C.

Liver Toxicity in Rats Fed A Mixture of Pcb Congeners and 2,3,7,8-Tcdd

1997 ◽  
Vol 3 (S2) ◽  
pp. 51-52
Author(s):  
B.J. Cornell ◽  
A. Singh ◽  
I. Chu
Keyword(s):  
Aromatic Compounds ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Morphological Changes ◽  
Test Substance ◽  
Sprague Dawley ◽  
Morphological Alterations ◽  
Pcb Congeners ◽  
Transmission Electron ◽  
Sprague Dawley Rats

Polyhalogenated aromatic compounds such as polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins (PCDDs) continue to be environmental contaminants because of their bioaccumulation in the food chain and high resistance to biodegradation. The current study was undertaken to determine if a mixture of PCB congeners (WHO-IPCS) were interactive with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in producing morphological changes in the rat liver. Both compounds are known to produce a broad range of biochemical and morphological alterations including enzyme induction.Groups (N=5) of female Sprague-Dawley rats were administered TCDD (0, 2.5, 25, 250, 1000 ng/kg bw/day) or PCB (0, 2, 20 μg/kg bw/day) alone, or in combination with each concentration of both compounds. Incorrect concentrations were published in a previous abstract. The test substance was mixed with corn oil and given by gavage at 2 ml/kg daily for 28 days. At the end of the experiment, the rats were killed and liver samples were prepared for transmission electron microscopy.

Ultrastructural changes of Basolateral Amygdaloid nuclei in the Sprague Dawley rats brain following exposure to naphthalene balls

2021 ◽  
Vol 12 (2) ◽  
pp. 1272-1275
Author(s):  
Angu Bala Ganesh K S V ◽  
Sujeet Shekhar Sinha ◽  
Kesavi Durairaj ◽  
Abdul Sahabudeen K
Keyword(s):  
Structural Changes ◽  
Corn Oil ◽  
Chromatin Condensation ◽  
Ultrastructural Changes ◽  
High Dose ◽  
Model Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Brain ◽  
Amygdaloid Nuclei

Naphthalene is a bicyclic aromatic constituent commonly used in different domestic and marketable applications comprising soil fumigants, lavatory scent disks and mothballs. Accidentally, workers, children and animals are exposed to naphthalene mothballs, so there is a need to study the pathology behind this chemical toxicity. The current study was carried out to assess the ultra structural changes of basolateral amygdaloid nuclei in the Sprague Dawley rats brain in association to naphthalene toxicity. The toxicity model group was administered with naphthalene (200 and 400mg) using corn oil as a vehicle for 28 days. The post delayed toxicity of naphthalene high dose ingestion was also assessed in rats. After the experimental period, the brain tissue was processed to observe the ultra structural changes using a transmission electron microscope. The alterations in cell organelles, nuclei damage, mitochondrial swelling, chromatin condensation suggested naphthalene induced damage in the neurons of the basolateral amygdala of the brain in the toxicity model group. These experimental trials provide information about the alert of mothball usage in the home and identify risks linked with accidental exposure and misuse.

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