On the Mechanism of Isoosmotic Transport of Fluid Across the Small Intestine. The Effect of the Staverman Reflection Coefficient of the Solute Used to Increase the Osmolality of the Mucosal Solution on the Composition of the Absorbate

1974 ◽  
Vol 52 (1) ◽  
pp. 96-104 ◽  
Author(s):  
I. T. Beck ◽  
P. K. Dinda
Keyword(s):  
Small Intestine ◽  
Reflection Coefficient ◽  
Solute Transport ◽  
Linear Increase ◽  
Water Soluble ◽  
Potential Difference ◽  
Reflection Coefficients ◽  
Serosal Side ◽  
Linear Decrease ◽  
Erythritol And Mannitol

The objective of the present study was to investigate the mechanism by which the fluid transported across the small intestine becomes isoosmotic with the mucosal solution. The osmolality of the mucosal solution of a unilateral preparation (the serosal side was not bathed) was increased with water soluble, nonelectrolyte solutes of different Staverman reflection coefficients (σ), and the composition of the undiluted absorbate was studied. The solutes ('hyperosmotic agents') used were formamide, urea, erythritol, and mannitol. In a series of separate experiments, utilizing a method based on potential difference depression, we found that the σ's of these solutes in the hamster small intestine were 0.26, 0.85, 0.93, and 1.00, respectively. Our data on transport across the unilateral preparation showed that when the intestine was incubated in an isotonic mucosal solution (Krebs–Ringer bicarbonate solution containing 10 mM glucose, 292 mosmol/kg), the absorbate was essentially isotonic, although its composition was significantly different from that of the mucosal solution. When the osmolality of the mucosal solution was increased to 342, 392, or to 442 mosmol/kg with any of the hyperosmotic agents, the absorbate was always isoosmotic with the mucosal solution. With formamide (low σ) as the hyperosmotic agent, the concentration of electrolytes and glucose in the absorbate was the same as that in the absorbate of the control preparation (incubated in the isotonic mucosal solution), and the transported fluid became isoosmotic with the mucosal solution exclusively due to the presence of formamide in the absorbate. When the osmolality of the mucosal solution was increased with urea, erythritol, or mannitol, the concentration of these hyperosmotic agents in the absorbate decreased linearly with the increase in their σ, and the absorbate became isoosmotic with the mucosal solution due to a linear increase in the concentration of electrolytes and glucose. In addition, we found that the transport of fluid, solutes, and fluid/solute (ml/mmol per gram dry intestine) decreased linearly with the increase in σ of the hyperosmotic agents. Further calculation of the data showed that the increase in the concentration of electrolytes and glucose with the increase in σ of the hyperosmotic agents was almost exclusively due to a linear decrease in fluid/solute transport.

On the Mechanism of Isosmotic Transport Across the Small Intestine. The Composition of the Absorbate Transported from a Mucosal Solution made Hypertonic by the Addition of Mannitol

1973 ◽  
Vol 51 (2) ◽  
pp. 130-138 ◽  
Author(s):  
P. K. Dinda ◽  
Marjorie Beck ◽  
I. T. Beck
Keyword(s):  
Small Intestine ◽  
Solute Transport ◽  
Fluid Transport ◽  
Linear Increase ◽  
Mucosal Surface ◽  
Bathing Solution ◽  
Serosal Side ◽  
Hypertonic Solutions ◽  
Mucosal Side

Experiments were performed to investigate the mechanism by which the fluid transported across the small intestine becomes isosmotic with the mucosal solution when the latter is made hypertonic by the addition of a poorly absorbed substance. The mid small intestine of the hamster was isolated and everted. The mucosal surface of the control preparation was bathed with an isotonic Krebs–Ringer bicarbonate solution containing 10 mM glucose (KRBSG) (292 mOsmol/kg). The mucosal surface of the experimental preparations was bathed with one of the hypertonic solutions. These hypertonic solutions were prepared by the addition of 1, 50, 100, or 150 mM mannitol to KRBSG. No bathing solution was placed on the serosal side of these preparations, so that the fluid transported across the intestine, undiluted by a bathing solution (absorbate), could be collected on the serosal side. Results show that the absorbate was always isosmotic with the mucosal solution. In the experimental preparations, as compared to the absorption of other solutes, mannitol was poorly absorbed, even though its concentration in the absorbate sometimes reached 35% of its concentration in the mucosal solution. The small amount of mannitol found in the absorbate was never sufficient to render the absorbates isosmotic with hypertonic mucosal solutions. The absorbate became isosmotic with the mucosal solution due to an increased concentration of other solutes transported from the mucosal side. Increasing the osmolality of the mucosal solution progressively from 292 to 442 mOsmol/kg caused a linear increase in the concentration of Na, K, glucose, and mannitol in the absorbate, but, except for mannitol, the net amount of these substances transported (per gram dry tissue) to the serosal side decreased linearly. The transport of fluid also decreased. Our data further indicate that the ratio of fluid and solute transport progressively decreased with the increase in osmolality of the mucosal solution. Therefore, it appears that when the mucosal solution is made hypertonic by the addition of a poorly absorbed substance, the absorbate becomes isosmotic with the hypertonic mucosal solution by means of a greater diminution of fluid transport than of solute transport.

Effects of metabolic disturbances on potential difference across intestinal wall of rat

1963 ◽  
Vol 204 (1) ◽  
pp. 105-108 ◽  
Author(s):  
Masashi Sawada ◽  
Tomoaki Asano
Keyword(s):  
Small Intestine ◽  
Low Temperature ◽  
Intestinal Wall ◽  
Potential Difference ◽  
Rapid Decline ◽  
Serosal Side ◽  
Metabolic Disturbances ◽  
Temperature Dependent ◽  
Mucosal Side

The potential difference across the wall of the small intestine was determined in vitro under a variety of conditions using rats. When the normal Ringer's containing 200 mg/100 ml glucose was applied on both sides of the wall, the potential difference attained 5–9 mv, the serosal side being positive. The potential difference was temperature dependent, becoming reduced at low temperature, the temperature coefficient being 1.7 between 40 and 34 C. The potential difference was inhibited with 0.1 mm monoiodoacetic acid, 1 mm sodium azide, 0.1 mm dinitrophenol, and 50 µm ouabain applied on the mucosal side. Withdrawal and restitution of 200 mg/100 ml glucose on the mucosal side induced a rapid decline and recovery of the potential difference. The lowered potential difference was partially recovered by 200 mg/100 ml galactose but not by sorbitol.

scholarly journals 146 Nutritional Advances in Fetal and neonatal development: effect of fatty acid supplementation

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 121-122
Author(s):  
Alejandro E Relling
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Plasma Insulin ◽  
Linear Increase ◽  
Late Gestation ◽  
Insulin Concentration ◽  
Plasma Insulin Concentration ◽  
Pufa Supplementation ◽  
Epa And Dha ◽  
Offspring Growth

Abstract Data from a series of experiments demonstrates that maternal supply of polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), during late gestation affects offspring growth. The increase in growth is independent on the fatty acid supplemented during the growing or finishing phase of the offspring; but it is sex dependent. Dam PUFA supplementation increases wether growth. Supplementation with EPA and DHA to pregnant ewes and to their offspring after weaning showed a treatment interaction in mRNA concentration of hypothalamic neuropeptides associated with dry matter intake (DMI) regulation. A dose increased in EPA and DHA in pregnant ewe diets shows a linear increase in growth, but a quadratic change in DMI or feed efficiency; growth was associated with a linear increase in plasma glucose concentration and a linear decrease in plasma ghrelin concentration. In lambs born from ewes supplemented with different sources of FA during a glucose tolerance test; males’ plasma insulin concentration increased as FA unsaturation degree increased in the dam diet, the opposite happened with females’ plasma insulin concentration. Recent data from our lab showed that the supplementation with EPA and DHA during the last third of gestation to pregnant ewes increased liver and small intestine global DNA methylation and small intestine transporters for amino acids in the fetus. Despite EPA and DHA during late gestation increase growth in the offspring; when EPA and DHA were supplemented in early gestation, offspring growth was lesser that lambs born from ewes supplemented a saturated and monounsaturated lipid. The reason for the difference in results it is not clear. However, more studies focusing in some aspect of the biology will help to understand what specific fatty acid needs to be supplemented at different stages of gestation to improve offspring growth.

Basic determinants of epicardial transudation

1997 ◽  
Vol 273 (3) ◽  
pp. H1408-H1414 ◽  
Author(s):  
R. H. Stewart ◽  
D. A. Rohn ◽  
S. J. Allen ◽  
G. A. Laine
Keyword(s):  
Reflection Coefficient ◽  
Coronary Sinus ◽  
Myocardial Edema ◽  
Hydraulic Conductance ◽  
Linear Increase ◽  
Left Ventricular ◽  
Edema Formation ◽  
Anesthetized Dogs ◽  
The Difference ◽  
Osmotic Reflection Coefficient

Myocardial edema formation, which has been shown to compromise cardiac function, and increased epicardial transudation (pericardial effusion) have been shown to occur after elevation of myocardial venous and lymphatic outflow pressures. The purposes of this study were to estimate the hydraulic conductance and osmotic reflection coefficient for the epicardium and to determine the effect of coronary sinus hypertension and cardiac lymphatic obstruction on epicardial fluid flux (JV,e/Ae). A Plexiglas hemispheric capsule was attached to the left ventricular epicardial surface of anesthetized dogs. JV,e/Ae was determined over 30-min periods for three intracapsular pressures (-5, -15, and -25 mmHg) and two intracapsular solutions exerting colloid osmotic pressures of 7.0 and 2.0 mmHg. Hydraulic conductance was estimated to be 3.7 +/- 0.5 microliters.h-1.cm-2.mmHg-1. An osmotic reflection coefficient of 0.9 was calculated from the difference in JV,e/Ae of 16.5 +/- 8.4 microliters.h-1.cm-2 between the two solutions. Graded coronary sinus hypertension induced a linear increase in JV,e/Ae, which was significantly greater in dogs without cardiac lymphatic occlusion than in those with occlusion.

LOOP ANTENNA WITH A SEMICONDUCTOR ELEMENT

2021 ◽  
Author(s):  
П.А. ТИТОВЕЦ ◽  
А.И. САТТАРОВА ◽  
А.А. ПИЩЕРКОВ ◽  
Н.С. БЕКУШЕВ
Keyword(s):  
Laser Radiation ◽  
Reflection Coefficient ◽  
Copper Foil ◽  
Loop Antenna ◽  
Laser Source ◽  
Reflection Coefficients ◽  
Building Element ◽  
External Contour ◽  
In Series ◽  
Loop Antennas

Представлены результаты исследований рамочной антенны, в которой подстроечным элементом является фоторезистор, управляемый лазерным излучением. Показано, что использование фоторезистора как элемента внешнего контура рамочной антенны, включенного последовательно, позволяет изменять согласование рамочной антенны с помощью внешнего лазерного источника. Представлены результаты исследований характеристик коэффициента передачи рамочных антенн, состоящих из медной фольги на диэлектрической основе и полупроводникового элемента. Установлено, что при изменении интенсивности лазерного излучения, падающего на полупроводниковый элемент-фоторезистор, изменяется коэффициент отражения рамочной антенны. В диапазоне от 10 МГц до 18ГГц получены зависимости коэффициентов отражения (Su)рамочных антенн с полупроводниковым элементом. Проведено сравнение рамочной антенны и рамочной антенны с фоторезистором. The results of an experiment with a loop antenna, in which the building element is a photoresistor controlled by laser radiation, are presented. It is shown that the use of a photoresistor as an element of the external contour of a loop antenna connected in series makes it possible to change the matching of the loop antenna due to an external laser source. The results of studies of the characteristics of the transmission coefficient of loop antennas consisting of a dielectric copper foil and a semiconductor element are presented. It was found that when the intensity of the laser radiation incident on the semiconductor element-photoresistor changes, the reflection coefficient of the frame antenna changes. In the range of 10 MHz-18 GHz, the dependences of the reflection coefficients (S11) of loop antennas with a semiconductor element are obtained. A comparison is made between a loop antenna and a loop antenna with a photoresistor.

Measurement of osmotic reflection coefficient for small molecules in cat hindlimbs

1989 ◽  
Vol 256 (1) ◽  
pp. H282-H290 ◽  
Author(s):  
M. B. Wolf ◽  
P. D. Watson
Keyword(s):  
Skeletal Muscle ◽  
Reflection Coefficient ◽  
Small Molecules ◽  
Osmotic Pressure ◽  
Maximum Rate ◽  
Reflection Coefficients ◽  
Flow Rates ◽  
Perfusate Flow ◽  
Arterial Inflow ◽  
Osmotic Reflection Coefficient

Capillary osmotic reflection coefficients (sigma) for NaCl, urea, sucrose, and raffinose were measured in the isolated, perfused cat hindlimb using the osmotic transient technique. sigma were determined from the ratio of the maximum rate of transcapillary absorption [delta Jv(max)] to the increase in the osmotic pressure (25-35 mosmol/kg H2O) in the arterial inflow (delta pi a) produced by adding one of the molecules to an albumin-electrolyte perfusate containing isoproterenol (greater than 10(-7) M). delta Jv (max) was determined from organ weight and delta pi a from perfusate osmolalities. For each molecule, the delta Jv(max)/delta pi a ratio increased monotonically with perfusate flow rates (Q) to Q greater than 100 ml.min-1.100 g-1. This ratio was independent of the size of the delta pi a. Apparent sigma values were calculated by dividing these ratios by the capillary hydraulic capacity determined in other studies. At low Q, apparent sigma was comparable to the approximately 0.1 values found by others in skeletal muscle. At the highest Q, apparent sigma for these molecules were at least 0.5. These data are consistent with at least 50% of transcapillary water flow moving through a water-exclusive pathway.

Protein osmotic pressure gradients and microvascular reflection coefficients

1997 ◽  
Vol 273 (2) ◽  
pp. H997-H1002 ◽  
Author(s):  
R. E. Drake ◽  
S. Dhother ◽  
R. A. Teague ◽  
J. C. Gabel
Keyword(s):  
Reflection Coefficient ◽  
Pressure Gradient ◽  
Osmotic Pressure ◽  
Reflection Coefficients ◽  
Pressure Gradients ◽  
Fluid Filtration ◽  
The Mean ◽  
Osmotic Reflection Coefficient ◽  
New Equation ◽  
Osmotic Pressure Gradient

Microvascular membranes are heteroporous, so the mean osmotic reflection coefficient for a microvascular membrane (sigma d) is a function of the reflection coefficient for each pore. Investigators have derived equations for sigma d based on the assumption that the protein osmotic pressure gradient across the membrane (delta II) does not vary from pore to pore. However, for most microvascular membranes, delta II probably does vary from pore to pore. In this study, we derived a new equation for sigma d. According to our equation, pore-to-pore differences in delta II increase the effect of small pores and decrease the effect of large pores on the overall membrane osmotic reflection coefficient. Thus sigma d for a heteroporous membrane may be much higher than previously derived equations indicate. Furthermore, pore-to-pore delta II differences increase the effect of plasma protein osmotic pressure to oppose microvascular fluid filtration.

Boundary conditions for the numerical solution of wave propagation problems

Geophysics ◽  
1978 ◽  
Vol 43 (6) ◽  
pp. 1099-1110 ◽  
Author(s):  
Albert C. Reynolds
Keyword(s):  
Wave Propagation ◽  
Reflection Coefficient ◽  
Finite Difference ◽  
Boundary Conditions ◽  
Numerical Solution ◽  
Neumann Boundary ◽  
Synthetic Seismograms ◽  
Neumann Boundary Conditions ◽  
Numerical Calculations ◽  
Reflection Coefficients

Many finite difference models in use for generating synthetic seismograms produce unwanted reflections from the edges of the model due to the use of Dirichlet or Neumann boundary conditions. In this paper we develop boundary conditions which greatly reduce this edge reflection. A reflection coefficient analysis is given which indicates that, for the specified boundary conditions, smaller reflection coefficients than those obtained for Dirichlet or Neumann boundary conditions are obtained. Numerical calculations support this conclusion.

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