PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: I. STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1963 ◽  
Vol 41 (1) ◽  
pp. 985-991 ◽  
Author(s):  
Robert F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Nucleic Acids ◽  
Mammary Tumor ◽  
Mammary Tumors ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Phosphorus Metabolism ◽  
Time Intervals ◽  
Mammary Tumor Virus ◽  
Target Organs ◽  
Tumor Virus

Studies of phosphorus metabolism, using P32-injected intraperitoneally into mice susceptible to mammary tumor induction by the mammary tumor virus, were conducted at time intervals corresponding to 24, 31, 64 days of age of the animals, and 10 months and older when mammary tumors developed. Chemical and radioactivity measurements of labelled phospholipides, nucleic acids, and phosphoproteins in liver, kidney, spleen, and mammary tissue were obtained from strain A (high-cancer strain) and BALB/c females (low-cancer strain). Both high- and low-cancer strains exhibited uniform patterns of phosphorus metabolism in non-target organs throughout the time periods selected. There are significant differences in phosphorus metabolism between the two strains which may be due to a combination of virus and genetic factors. In strain A females, 10 months and older, bearing mammary tumors, the mammary glands without tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to mammary glands of identical females that had not yet developed mammary tumors.

PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: I. STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1963 ◽  
Vol 41 (4) ◽  
pp. 985-991 ◽  
Author(s):  
Robert F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Nucleic Acids ◽  
Mammary Tumor ◽  
Mammary Tumors ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Phosphorus Metabolism ◽  
Time Intervals ◽  
Mammary Tumor Virus ◽  
Target Organs ◽  
Tumor Virus

Studies of phosphorus metabolism, using P32-injected intraperitoneally into mice susceptible to mammary tumor induction by the mammary tumor virus, were conducted at time intervals corresponding to 24, 31, 64 days of age of the animals, and 10 months and older when mammary tumors developed. Chemical and radioactivity measurements of labelled phospholipides, nucleic acids, and phosphoproteins in liver, kidney, spleen, and mammary tissue were obtained from strain A (high-cancer strain) and BALB/c females (low-cancer strain). Both high- and low-cancer strains exhibited uniform patterns of phosphorus metabolism in non-target organs throughout the time periods selected. There are significant differences in phosphorus metabolism between the two strains which may be due to a combination of virus and genetic factors. In strain A females, 10 months and older, bearing mammary tumors, the mammary glands without tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to mammary glands of identical females that had not yet developed mammary tumors.

PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: II. IN VIVO AND IN VITRO STUDIES OF THE MILK AND MAMMARY GLAND LIPIDES OF STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1965 ◽  
Vol 43 (3) ◽  
pp. 297-307 ◽  
Author(s):  
James Newland ◽  
R. F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Fatty Acid Composition ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Nucleic Acids ◽  
Acid Composition ◽  
Mammary Tumor ◽  
Mammary Glands ◽  
Phosphorus Metabolism ◽  
Mammary Tumor Virus ◽  
Tumor Virus

Studies of phosphorus metabolism in the mammary glands of C3HZ, C3HZb, and A strain female mice have been carried out using P32-labeled inorganic phosphate. Chemical and radioactivity measurements of labeled phospholipides, nucleic acids, and phosphoproteins of mammary tissue were obtained. The fatty acid composition of the chromatographed and separated phospholipides, neutral fat, and free fatty acids of milk of high-cancer-strain (C3HZ and A) females were compared to patterns obtained for low-cancer-strain (C3HZb) females. The lipide pattern of the mammary tumor virus fraction obtained by ultra-centrifugation was investigated and compared to the results found for mammary gland and milk. The C3HZ female bearing mammary tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to the genetically similar, low-cancer-strain C3HZb females and to identical C3HZ females that had not yet developed mammary tumors. Investigation of the phospholipide fatty acid composition of the milk and mammary glands of high- and low-cancer strains revealed further marked differences with respect to the palmitoleic and linoleic acid content.

scholarly journals A Novel Mechanism of Resistance to Mouse Mammary Tumor Virus Infection

2000 ◽  
Vol 74 (6) ◽  
pp. 2752-2759 ◽  
Author(s):  
Tatyana V. Golovkina
Keyword(s):  
Mammary Gland ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Inbred Mice ◽  
Tumor Development ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

ABSTRACT Exogenous mouse mammary tumor virus (MMTV) is carried from the gut of suckling pups to the mammary glands by lymphocytes and induces mammary gland tumors. MMTV-induced tumor incidence in inbred mice of different strains ranges from 0 to as high as 100%. For example, mice of the C3H/HeN strain are highly susceptible, whereas mice of the I/LnJ strain are highly resistant. Of the different factors that together determine the susceptibility of mice to development of MMTV-induced mammary tumors, genetic elements play a major role, although very few genes that determine a susceptibility-resistance phenotype have been identified so far. Our data indicate that MMTV fails to infect mammary glands in I/LnJ mice foster nursed on viremic C3H/HeN females, even though the I/LnJ mammary tissue is not refractory to MMTV infection. Lymphocytes from fostered I/LnJ mice contained integrated MMTV proviruses and shed virus but failed to establish infection in the mammary glands of susceptible syngeneic (I × C3H.JK)F1 females. Based on the susceptible-resistant phenotype distribution in N2 females, both MMTV mammary gland infection and mammary gland tumor development in I/LnJ mice are controlled by a single locus.

scholarly journals Mouse Mammary Tumor Virus c-rel Transgenic Mice Develop Mammary Tumors

2003 ◽  
Vol 23 (16) ◽  
pp. 5738-5754 ◽  
Author(s):  
Raphaëlle Romieu-Mourez ◽  
Dong W. Kim ◽  
Sang Min Shin ◽  
Elizabeth G. Demicco ◽  
Esther Landesman-Bollag ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Cyclin D1 ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Tumors ◽  
Mammary Glands ◽  
Breast Cancers ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

ABSTRACT Amplification, overexpression, or rearrangement of the c-rel gene, encoding the c-Rel NF-κB subunit, has been reported in solid and hematopoietic malignancies. For example, many primary human breast cancer tissue samples express high levels of nuclear c-Rel. While the Rev-T oncogene v-rel causes tumors in birds, the ability of c-Rel to transform in vivo has not been demonstrated. To directly test the role of c-Rel in breast tumorigenesis, mice were generated in which overexpression of mouse c-rel cDNA was driven by the hormone-responsive mouse mammary tumor virus long terminal repeat (MMTV-LTR) promoter, and four founder lines identified. In the first cycle of pregnancy, the expression of transgenic c-rel mRNA was observed, and levels of c-Rel protein were increased in the mammary gland. Importantly, 31.6% of mice developed one or more mammary tumors at an average age of 19.9 months. Mammary tumors were of diverse histology and expressed increased levels of nuclear NF-κB. Analysis of the composition of NF-κB complexes in the tumors revealed aberrant nuclear expression of multiple subunits, including c-Rel, p50, p52, RelA, RelB, and the Bcl-3 protein, as observed previously in human primary breast cancers. Expression of the cancer-related NF-κB target genes cyclin D1, c-myc, and bcl-xl was significantly increased in grossly normal transgenic mammary glands starting the first cycle of pregnancy and increased further in mammary carcinomas compared to mammary glands from wild-type mice or virgin transgenic mice. In transient transfection analysis in untransformed breast epithelial cells, c-Rel-p52 or -p50 heterodimers either potently or modestly induced cyclin D1 promoter activity, respectively. Lastly, stable overexpression of c-Rel resulted in increased cyclin D1 and NF-κB p52 and p50 subunit protein levels. These results indicate for the first time that dysregulated expression of c-Rel, as observed in breast cancers, is capable of contributing to mammary tumorigenesis.

Stability of Characters of Mammary Tumors in Balb/cfRIII Mice

1980 ◽  
Vol 66 (4) ◽  
pp. 439-444 ◽  
Author(s):  
Raffaele Pingitore
Keyword(s):  
Mammary Tumor ◽  
Morphological Characteristics ◽  
Mammary Tumors ◽  
Biological Properties ◽  
Mammary Tumor Virus ◽  
Tumor Virus

The biological and morphological characteristics of mammary tumors in BALB/c mice infected with RIII mammary tumor virus (MTV) by foster nursing have substantially kept stable and unchanged after 20 years, 53 inbred generations and transfer of the strain from Perugia to Pisa. This suggests that the causal MTV keeps unaltered in time its biological properties.

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