PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: II. IN VIVO AND IN VITRO STUDIES OF THE MILK AND MAMMARY GLAND LIPIDES OF STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1965 ◽  
Vol 43 (3) ◽  
pp. 297-307 ◽  
Author(s):  
James Newland ◽  
R. F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Fatty Acid Composition ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Nucleic Acids ◽  
Acid Composition ◽  
Mammary Tumor ◽  
Mammary Glands ◽  
Phosphorus Metabolism ◽  
Mammary Tumor Virus ◽  
Tumor Virus

Studies of phosphorus metabolism in the mammary glands of C3HZ, C3HZb, and A strain female mice have been carried out using P32-labeled inorganic phosphate. Chemical and radioactivity measurements of labeled phospholipides, nucleic acids, and phosphoproteins of mammary tissue were obtained. The fatty acid composition of the chromatographed and separated phospholipides, neutral fat, and free fatty acids of milk of high-cancer-strain (C3HZ and A) females were compared to patterns obtained for low-cancer-strain (C3HZb) females. The lipide pattern of the mammary tumor virus fraction obtained by ultra-centrifugation was investigated and compared to the results found for mammary gland and milk. The C3HZ female bearing mammary tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to the genetically similar, low-cancer-strain C3HZb females and to identical C3HZ females that had not yet developed mammary tumors. Investigation of the phospholipide fatty acid composition of the milk and mammary glands of high- and low-cancer strains revealed further marked differences with respect to the palmitoleic and linoleic acid content.

PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: I. STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1963 ◽  
Vol 41 (1) ◽  
pp. 985-991 ◽  
Author(s):  
Robert F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Nucleic Acids ◽  
Mammary Tumor ◽  
Mammary Tumors ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Phosphorus Metabolism ◽  
Time Intervals ◽  
Mammary Tumor Virus ◽  
Target Organs ◽  
Tumor Virus

Studies of phosphorus metabolism, using P32-injected intraperitoneally into mice susceptible to mammary tumor induction by the mammary tumor virus, were conducted at time intervals corresponding to 24, 31, 64 days of age of the animals, and 10 months and older when mammary tumors developed. Chemical and radioactivity measurements of labelled phospholipides, nucleic acids, and phosphoproteins in liver, kidney, spleen, and mammary tissue were obtained from strain A (high-cancer strain) and BALB/c females (low-cancer strain). Both high- and low-cancer strains exhibited uniform patterns of phosphorus metabolism in non-target organs throughout the time periods selected. There are significant differences in phosphorus metabolism between the two strains which may be due to a combination of virus and genetic factors. In strain A females, 10 months and older, bearing mammary tumors, the mammary glands without tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to mammary glands of identical females that had not yet developed mammary tumors.

PHOSPHORUS METABOLISM IN VIRAL-INDUCED NEOPLASIA: I. STRAINS OF MICE SUSCEPTIBLE TO THE MAMMARY TUMOR VIRUS

1963 ◽  
Vol 41 (4) ◽  
pp. 985-991 ◽  
Author(s):  
Robert F. McGregor ◽  
W. E. Cornatzer
Keyword(s):  
Nucleic Acids ◽  
Mammary Tumor ◽  
Mammary Tumors ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Phosphorus Metabolism ◽  
Time Intervals ◽  
Mammary Tumor Virus ◽  
Target Organs ◽  
Tumor Virus

Studies of phosphorus metabolism, using P32-injected intraperitoneally into mice susceptible to mammary tumor induction by the mammary tumor virus, were conducted at time intervals corresponding to 24, 31, 64 days of age of the animals, and 10 months and older when mammary tumors developed. Chemical and radioactivity measurements of labelled phospholipides, nucleic acids, and phosphoproteins in liver, kidney, spleen, and mammary tissue were obtained from strain A (high-cancer strain) and BALB/c females (low-cancer strain). Both high- and low-cancer strains exhibited uniform patterns of phosphorus metabolism in non-target organs throughout the time periods selected. There are significant differences in phosphorus metabolism between the two strains which may be due to a combination of virus and genetic factors. In strain A females, 10 months and older, bearing mammary tumors, the mammary glands without tumors exhibited increased synthesis of phospholipides and nucleic acids when compared to mammary glands of identical females that had not yet developed mammary tumors.

scholarly journals A Novel Mechanism of Resistance to Mouse Mammary Tumor Virus Infection

2000 ◽  
Vol 74 (6) ◽  
pp. 2752-2759 ◽  
Author(s):  
Tatyana V. Golovkina
Keyword(s):  
Mammary Gland ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Inbred Mice ◽  
Tumor Development ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

ABSTRACT Exogenous mouse mammary tumor virus (MMTV) is carried from the gut of suckling pups to the mammary glands by lymphocytes and induces mammary gland tumors. MMTV-induced tumor incidence in inbred mice of different strains ranges from 0 to as high as 100%. For example, mice of the C3H/HeN strain are highly susceptible, whereas mice of the I/LnJ strain are highly resistant. Of the different factors that together determine the susceptibility of mice to development of MMTV-induced mammary tumors, genetic elements play a major role, although very few genes that determine a susceptibility-resistance phenotype have been identified so far. Our data indicate that MMTV fails to infect mammary glands in I/LnJ mice foster nursed on viremic C3H/HeN females, even though the I/LnJ mammary tissue is not refractory to MMTV infection. Lymphocytes from fostered I/LnJ mice contained integrated MMTV proviruses and shed virus but failed to establish infection in the mammary glands of susceptible syngeneic (I × C3H.JK)F1 females. Based on the susceptible-resistant phenotype distribution in N2 females, both MMTV mammary gland infection and mammary gland tumor development in I/LnJ mice are controlled by a single locus.

scholarly journals Dietary effects of linseed on fatty acid composition of milk and on liver, adipose and mammary gland metabolism of periparturient dairy cows

2013 ◽  
Vol 97 ◽  
pp. 89-104 ◽  
Author(s):  
N. Mach ◽  
R. L. G. Zom ◽  
H. C. A. Widjaja ◽  
P. G. van Wikselaar ◽  
R. E. Weurding ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Dairy Cows ◽  
Acid Composition ◽  
Dietary Effects

An entire functional mammary gland may comprise the progeny from a single cell

Development ◽  
1998 ◽  
Vol 125 (10) ◽  
pp. 1921-1930 ◽  
Author(s):  
E.C. Kordon ◽  
G.H. Smith
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mammary Gland ◽  
Mammary Tumor ◽  
Mouse Mammary Tumor Virus ◽  
Mammary Epithelial ◽  
Self Renewal ◽  
Mammary Tumor Virus ◽  
Mouse Mammary Tumor ◽  
Tumor Virus

Any epithelial portion of a normal mouse mammary gland can reproduce an entire functional gland when transplanted into an epithelium-free mammary fat pad. Mouse mammary hyperplasias and tumors are clonal dominant populations and probably represent the progeny of a single transformed cell. Our study provides evidence that single multipotent stem cells positioned throughout the mature fully developed mammary gland have the capacity to produce sufficient differentiated progeny to recapitulate an entire functional gland. Our evidence also demonstrates that these stem cells are self-renewing and are found with undiminished capacities in the newly regenerated gland. We have taken advantage of an experimental model where mouse mammary tumor virus infects mammary epithelial cells and inserts a deoxyribonucleic acid copy(ies) of its genome during replication. The insertions occur randomly within the somatic genome. CzechII mice have no endogenous nucleic acid sequence homology with mouse mammary tumor virus; therefore all viral insertions may be detected by Southern analysis provided a sufficient number of cells contain a specific insertional event. Transplantation of random fragments of infected CzechII mammary gland produced clonal-dominant epithelial populations in epithelium-free mammary fat pads. Serial transplantation of pieces of the clonally derived outgrowths produced second generation glands possessing the same viral insertion sites providing evidence for self-renewal of the original stem cell. Limiting dilution studies with cell cultures derived from third generation clonal outgrowths demonstrated that three multipotent but distinct mammary epithelial progenitors were present in clonally derived mammary epithelial populations. Estimation of the potential number of multipotent epithelial cells that may be evolved from an individual mammary-specific stem cell by self-renewal is in the order of 10(12)-10(13). Therefore, one stem cell might easily account for the renewal of mammary epithelium over several transplant generations.

scholarly journals Participation of mammary tumor virus in mammary gland sensitivity to prolactin in mice

1972 ◽  
Vol 43 (9) ◽  
pp. 519-523
Author(s):  
Hiroshi NAGASAWA ◽  
Reiko YANAI ◽  
Moriyoshi MIYAMOTO
Keyword(s):  
Mammary Gland ◽  
Mammary Tumor ◽  
Mammary Tumor Virus ◽  
Tumor Virus
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