THE SIGNIFICANCE OF HUMAN BLOOD TRYPSIN; DIRECT DETERMINATION OF THROMBIN AND PLASMIN IN HUMAN PLASMA

1962 ◽  
Vol 40 (1) ◽  
pp. 1725-1735
Author(s):  
Edward Ronwin
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Direct Determination ◽  
Precursor Protein ◽  
Common Precursor ◽  
Conversion System ◽  
Human Thrombin ◽  
Derivatives Of

The previously described method for human plasmin determination has been simplified and applied directly to plasma. A virtually identical method for the direct determination of human thrombin in plasma is also presented. Both procedures are quantitative and clinically feasible. The existence in plasma of a non-clotting, only slightly fibrinolytic, active tryptic enzyme, Human Blood Trypsin, is firmly established by the data. The suggestion is made that human blood trypsin may be the activator in the proenzyme to enzyme conversion system for both plasmin and thrombin. Further, both enzymes may arise from different derivatives of a common precursor protein.

THE SIGNIFICANCE OF HUMAN BLOOD TRYPSIN; DIRECT DETERMINATION OF THROMBIN AND PLASMIN IN HUMAN PLASMA

1962 ◽  
Vol 40 (12) ◽  
pp. 1725-1735 ◽  
Author(s):  
Edward Ronwin
Keyword(s):  
Human Plasma ◽  
Human Blood ◽  
Direct Determination ◽  
Precursor Protein ◽  
Common Precursor ◽  
Conversion System ◽  
Human Thrombin ◽  
Derivatives Of

The previously described method for human plasmin determination has been simplified and applied directly to plasma. A virtually identical method for the direct determination of human thrombin in plasma is also presented. Both procedures are quantitative and clinically feasible. The existence in plasma of a non-clotting, only slightly fibrinolytic, active tryptic enzyme, Human Blood Trypsin, is firmly established by the data. The suggestion is made that human blood trypsin may be the activator in the proenzyme to enzyme conversion system for both plasmin and thrombin. Further, both enzymes may arise from different derivatives of a common precursor protein.

Molecular Imprinted Graphene Oxide Nanocomposite for Optical Sensing of Nicotine in Human Blood Plasma

2020 ◽  
Vol 42 (6) ◽  
pp. 856-856
Author(s):  
Sehrish Qazi Sehrish Qazi ◽  
Huma Shaikh Huma Shaikh ◽  
Ayaz Ali Memon Ayaz Ali Memon ◽  
and Shahabuddin Memon and Shahabuddin Memon
Keyword(s):  
Graphene Oxide ◽  
Human Plasma ◽  
Human Blood ◽  
Optical Sensor ◽  
Single Layer ◽  
Human Blood Plasma ◽  
Buffer Concentration ◽  
Imprinted Polymer ◽  
Ft Ir

Among all psychotropic alkaloids, nicotine is more addictive, carcinogenic and capable of causing many health problems. This work is based on the development of highly robust, cheap, reliable, selective and sensitive nicotine imprinted graphene oxide nanocomposite (ImpGO nanocomposite) based optical sensor for determination of nicotine in human plasma. The ImpGO nanocomposite has been thoroughly characterized using different techniques i.e. FT-IR, SEM, TEM, Raman, etc. These characterizations revealed that ImpGO nanocomposite is comprised of single layer of graphene oxide successfully modified with imprinted polymer. The synthesized material was utilized to selectively determine nicotine using UV-vis spectrophotometer in BR buffer of 0.1 M at pH 3 and diluted human plasma. The effect of parameters such as buffer concentration, pH, amount of nanocomposite, etc on determination of nicotine using ImpGO nanocomposite were studied thoroughly. Thus, a sensitive optical method was developed for determination of nicotine in human plasma with linear range of 22-370 pM along with LOD and LOQ of 7 pM and 22 pM, respectively. The selectivity of sensor was evaluated using homologues of nicotine such as nicotine amide, caffeine and cotinine. The results obtained from biological samples showed that developed optical sensor is efficient in complex matrices of real sample.

Simultaneous Determination of Floctafenine and its Hydrolytic Degradation Product Floctafenic Acid Using Micellar Liquid Chromatography with Applications to Tablets and Human Plasma

2013 ◽  
Vol 96 (6) ◽  
pp. 1315-1324 ◽  
Author(s):  
Mohamed I Walash ◽  
Fathalla Belal ◽  
Nahed El-Enany ◽  
Manal Eid ◽  
Rania N El-Shaheny
Keyword(s):  
Liquid Chromatography ◽  
Human Plasma ◽  
Degradation Product ◽  
Mobile Phase ◽  
Sodium Dodecyl ◽  
Hydrolytic Degradation ◽  
Direct Determination ◽  
Micellar Liquid Chromatography ◽  
Main Metabolite

Abstract A stability-indicating micellar liquid chromatography (MLC) method was developed and validated for the assay of floctafenine (FLF) in the presence of its degradation product and main metabolite, floctafenic acid (FLA). The analysis was carried out on a CLC Shim-Pack octyl silane (C8) column (150 × 4.6 mm id, 5 μm particle size) using a micellar mobile phase consisting of 0.15 M sodium dodecyl sulfate, 10% n-propanol, and 0.3% triethylamine in 0.02 M orthophosphoric acid (pH = 3). The mobile phase was pumped at a flow rate of 1.0 mL/min with UV detection at 360 nm. The method showed good linearity for FLF and FLA over the concentration ranges of 0.5–25.0 and 0.4–10.0 μg/mL, with LODs of 0.16 and 0.12 μg/mL, respectively. The developed method was successfully applied to the determination of FLF in commercial dispersible tablets, with mean recovery of 98.87 ± 1.37%. Also, the proposed method was specific for the analysis of FLF in presence of the co-formulated drug thiocolchicoside in laboratory-prepared tablets, with mean recovery of 100.50 ± 1.07%. Statistical comparison of the results obtained by the proposed MLC method with those obtained by a comparison method showed good agreement. Moreover, the method was extended to study the degradation behavior of FLF under different International Conference on Harmonization recommended conditions such as alkaline, acidic, oxidative, thermal, and photolytic. The method was further applied for direct determination of FLA as the main metabolite of FLF in human plasma without prior extraction steps, with mean recovery of 110.50 ± 6.5%.

scholarly journals Direct determination of trans-resveratrol in human plasma by spectrofluorimetry and second-order standard addition

Talanta ◽  
2010 ◽  
Vol 82 (2) ◽  
pp. 640-645 ◽  
Author(s):  
Cristina D. Bernardes ◽  
Ronei J. Poppi ◽  
Marcelo M. Sena
Keyword(s):  
Human Plasma ◽  
Direct Determination ◽  
Standard Addition ◽  
Second Order

scholarly journals Direct Determination of Aluminium Concentration in Human Blood Through Laser Photoionization Spectroscopy

1984 ◽  
Vol 5 (1) ◽  
pp. 11-17 ◽  
Author(s):  
G. I. Bekov ◽  
V. S. Letokhov ◽  
V. N. Radayev
Keyword(s):  
Human Blood ◽  
Molecular Beam ◽  
Rydberg State ◽  
Analytical Procedure ◽  
Direct Determination ◽  
Aluminium Content ◽  
Electron Multiplier ◽  
Aluminium Concentration ◽  
Laser Photoionization

The results of aluminium content measurements in human blood by the method of laser stepwise photoionization of atoms in combination with vacuum thermal atomization of organic substance are presented. The analytical procedure was as follows. Dry blood residium obtained after drying of 40 μl natural blood was stepwise heated in vacuum to 1800℃. The aluminium atoms produced in an atomic-molecular beam were excited to a Rydberg state in two steps by laser radiation and then efficiently ionized by electric field pulse. The resulting ions were detected with an electron multiplier. The calibration curve obtained for aqueous solutions of AlCl3 was used for quantitative determination of aluminium content. The measured value of aluminium concentration in human blood came to (230 ± 50) ng/ml.

scholarly journals Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Le-Qian Hu ◽  
Chun-Ling Yin ◽  
Ya-Hui Du ◽  
Zhi-Peng Zeng
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Complex Analysis ◽  
Direct Determination ◽  
Second Order ◽  
Decomposition Algorithms ◽  
Statistical Comparison ◽  
Trilinear Decomposition ◽  
Second Order Calibration

A simple, rapid, and sensitive method for the simultaneous determination of vancomycin and cephalexin in human plasma was developed by using HPLC-DAD with second-order calibration algorithms. Instead of a completely chromatographic separation, mathematical separation was performed by using two trilinear decomposition algorithms, that is, PARAFAC-alternative least squares (PARAFAC-ALSs) and self-weight-alternative-trilinear-decomposition- (SWATLD-) coupled high-performance liquid chromatography with DAD detection. The average recoveries attained from PARAFAC-ALS and SWATLD with the factor number of 4 (N=4) were101±5% and102±4% for vancomycin, and96±3% and97±3% for cephalexininde in real human samples, respectively. The statistical comparison between PARAFAC-ALS and SWATLD is demonstrated to be similar. The results indicated that the combination of HPLC-DAD detection with second-order calibration algorithms is a powerful tool to quantify the analytes of interest from overlapped chromatographic profiles for complex analysis of drugs in plasma.

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