scholarly journals Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Le-Qian Hu ◽  
Chun-Ling Yin ◽  
Ya-Hui Du ◽  
Zhi-Peng Zeng
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Complex Analysis ◽  
Direct Determination ◽  
Second Order ◽  
Decomposition Algorithms ◽  
Statistical Comparison ◽  
Trilinear Decomposition ◽  
Second Order Calibration

A simple, rapid, and sensitive method for the simultaneous determination of vancomycin and cephalexin in human plasma was developed by using HPLC-DAD with second-order calibration algorithms. Instead of a completely chromatographic separation, mathematical separation was performed by using two trilinear decomposition algorithms, that is, PARAFAC-alternative least squares (PARAFAC-ALSs) and self-weight-alternative-trilinear-decomposition- (SWATLD-) coupled high-performance liquid chromatography with DAD detection. The average recoveries attained from PARAFAC-ALS and SWATLD with the factor number of 4 (N=4) were101±5% and102±4% for vancomycin, and96±3% and97±3% for cephalexininde in real human samples, respectively. The statistical comparison between PARAFAC-ALS and SWATLD is demonstrated to be similar. The results indicated that the combination of HPLC-DAD detection with second-order calibration algorithms is a powerful tool to quantify the analytes of interest from overlapped chromatographic profiles for complex analysis of drugs in plasma.

scholarly journals A Comparison of Second-Order Calibration Methods Applied to Excitation-Emission Matrix Fluorescence Data

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hui Chen ◽  
Zan Lin ◽  
Tong Wu ◽  
Chao Tan
Keyword(s):  
Performance Comparison ◽  
Second Order ◽  
Trilinear Decomposition ◽  
Excitation Emission Matrix ◽  
Calibration Methods ◽  
Mathematical Algorithms ◽  
Spectral Profiles ◽  
Second Order Calibration ◽  
Potential Tool

Due to the variety of second-order data being generated by modern instruments and various mathematical algorithms being available for analysis purposes, second-order calibration is gaining widespread acceptance by analytical community. It has the so-called second-order advantage; that is, it enables concentration and spectral profiles of sample components to be extracted even in the presence of unexpected interferences. A comprehensive performance comparison of alternating trilinear decomposition (ATLD) and its two variants, that is, alternating penalty trilinear decomposition (APTLD) and self-weighted trilinear decomposition (SWATLD), was presented in this paper. The experiment was based on the simultaneous determination of three dihydroxybenzenes, that is, catechol, hydroquinone, and resorcinol, by excitation-emission matrix fluorescence (EEMF) spectroscopy. Two special measures, that is, the consistency (COS) between the resolved and actual profiles and the mean of recovery, were used for evaluation. The optimal result was obtained by the APTLD model with five components. No perceptible difference on the speed of convergence was found. It indicates that EEMF linked with the APTLD algorithm can serve as a potential tool of quantifying dihydroxybenzenes simultaneously in environmental samples.

Determination of pesticides and metabolites in wine by high performance liquid chromatography and second-order calibration methods

2007 ◽  
Vol 1148 (2) ◽  
pp. 200-210 ◽  
Author(s):  
Jez Willian B. Braga ◽  
Carla B.G. Bottoli ◽  
Isabel C.S.F. Jardim ◽  
Héctor C. Goicoechea ◽  
Alejandro C. Olivieri ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Second Order ◽  
Calibration Methods ◽  
Second Order Calibration

scholarly journals Direct determination of trans-resveratrol in human plasma by spectrofluorimetry and second-order standard addition

Talanta ◽  
2010 ◽  
Vol 82 (2) ◽  
pp. 640-645 ◽  
Author(s):  
Cristina D. Bernardes ◽  
Ronei J. Poppi ◽  
Marcelo M. Sena
Keyword(s):  
Human Plasma ◽  
Direct Determination ◽  
Standard Addition ◽  
Second Order
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