THE EFFECTS OF SULPHUR DIOXIDE UPON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1962 ◽  
Vol 40 (1) ◽  
pp. 207-217 ◽  
Author(s):  
James R. Thompson ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Hela Cells ◽  
Sulphur Dioxide ◽  
Mouse Liver ◽  
Recovery Period ◽  
Inhibition Of Growth ◽  
Established Cell Lines ◽  
Established Cell ◽  
Stimulation Of

Studies have been made on the effects of SO2 and its salts on strain L, mouse liver, and HeLa cells. Of the cell lines tested, the HeLa cells seemed to be more sensitive to SO2 and its salts than the cells of mouse origin.Cells cultivated in "biological" medium grow in concentrations of gaseous SO2 up to 2000 p.p.m., although somewhat inhibited. Cells subjected to a concentration of 500 p.p.m. in this medium are not affected greatly and their growth is comparable to those cells in control cultures.The addition of various salts of SO2 (Na2SO4, Na2SO3, and NaHSO3) in concentrations from 10 to 200 mg% produced responses ranging from complete inhibition of growth (by 200 mg% NaHSO3) to apparent stimulation of growth by some concentrations of Na2SO4. Toxicity of these salts was in the order of NaHSO3 > Na2SO3 > Na2SO4.When cells in vitro are directly exposed to SO2 in specially designed culture flasks, strain L cells are apparently able to tolerate 5 p.p.m. SO2 for five 8-hour exposure intervals, provided a "recovery" period follows each exposure.Certain components of serum seem to play a very important role as protective agents in modifying the effect of gaseous SO2, possibly by combination.

THE EFFECTS OF SULPHUR DIOXIDE UPON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1962 ◽  
Vol 40 (2) ◽  
pp. 207-217 ◽  
Author(s):  
James R. Thompson ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Hela Cells ◽  
Sulphur Dioxide ◽  
Mouse Liver ◽  
Recovery Period ◽  
Inhibition Of Growth ◽  
Established Cell Lines ◽  
Established Cell ◽  
Stimulation Of

Studies have been made on the effects of SO2 and its salts on strain L, mouse liver, and HeLa cells. Of the cell lines tested, the HeLa cells seemed to be more sensitive to SO2 and its salts than the cells of mouse origin.Cells cultivated in "biological" medium grow in concentrations of gaseous SO2 up to 2000 p.p.m., although somewhat inhibited. Cells subjected to a concentration of 500 p.p.m. in this medium are not affected greatly and their growth is comparable to those cells in control cultures.The addition of various salts of SO2 (Na2SO4, Na2SO3, and NaHSO3) in concentrations from 10 to 200 mg% produced responses ranging from complete inhibition of growth (by 200 mg% NaHSO3) to apparent stimulation of growth by some concentrations of Na2SO4. Toxicity of these salts was in the order of NaHSO3 > Na2SO3 > Na2SO4.When cells in vitro are directly exposed to SO2 in specially designed culture flasks, strain L cells are apparently able to tolerate 5 p.p.m. SO2 for five 8-hour exposure intervals, provided a "recovery" period follows each exposure.Certain components of serum seem to play a very important role as protective agents in modifying the effect of gaseous SO2, possibly by combination.

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

THE EFFECTS OF NO2 AND SALTS OF NO2 UPON ESTABLISHED CELL LINES

1961 ◽  
Vol 39 (8) ◽  
pp. 1247-1255 ◽  
Author(s):  
Donald M. Pace ◽  
James R. Thompson ◽  
Byron Th. Aftonomos ◽  
Harald G. O. Holck
Keyword(s):  
Thin Film ◽  
Cell Lines ◽  
Hela Cells ◽  
Air Pollutants ◽  
Mouse Liver ◽  
The Other ◽  
Continuous Exposure ◽  
Established Cell Lines ◽  
Established Cell ◽  
Cell Strains

The effects of several concentrations of NO2, NaNO3, and NaNO2 respectively, upon strain L, mouse liver cells, and HeLa cells, were studied and a modified system designed to permit continuous exposure of cells to air pollutants is described. In NCTC medium 109 containing serum, cells tolerate concentrations of NO2 up to 4100 p.p.m. and some may even tolerate 8600 p.p.m. Removal of the serum lowers the lethal concentration of NO2 to less than 100 p.p.m. If the cells were covered only by a thin film of BSS (balanced salt solution) medium, a concentration of 100 p.p.m. NO2 proved toxic within [Formula: see text] hour. If, however, the NO2 concentration was reduced to 5 or 10 p.p.m., cells survived a daily 8-hour exposure but many, if not most, of the cells were dead after several days. The presence of as little as 25 mg% NaNO2 retarded proliferation. On the other hand, NaNO3 was tolerated well in the three cell lines tested; HeLa cells seemed to be the most sensitive of the cell strains with respect to these salts.

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (1) ◽  
pp. 299-304
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

Sensitivity to Amphotericin B of in vitro Established Cell Lines

Nature ◽  
1974 ◽  
Vol 247 (5441) ◽  
pp. 466-469 ◽  
Author(s):  
P. AMATI ◽  
C. LAGO
Keyword(s):  
Amphotericin B ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell

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Medicinal Plants ◽  
Essential Oils ◽  
Cell Lines ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Established Cell Lines ◽  
Established Cell

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The World ◽  
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In vitro cell culture is commonly applied in laboratories around the world. Cultured cells are either of primary origin or established cell lines. Such transformed cell lines are increasingly replaced...

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