scholarly journals Evaluation of essential oils from 22 Guatemalan medicinal plants for in vitro activity against cancer and established cell lines

2018 ◽  
Vol 12 (3) ◽  
pp. 42-49 ◽  
Author(s):  
Byron Miller Andrew ◽  
Gordon Cates Rex ◽  
O’Neill Kim ◽  
Alfonso Fuentes Soria Juan ◽  
Vicente Espinoza Luis ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Essential Oils ◽  
Cell Lines ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Established Cell Lines ◽  
Established Cell

Flavopiridol Induces Apoptosis in Chronic Lymphocytic Leukemia Cells Via Activation of Caspase-3 Without Evidence of bcl-2 Modulation or Dependence on Functional p53

Blood ◽  
1998 ◽  
Vol 92 (10) ◽  
pp. 3804-3816 ◽  
Author(s):  
John C. Byrd ◽  
Charlotte Shinn ◽  
Jamie K. Waselenko ◽  
Ephraim J. Fuchs ◽  
Teresa A. Lehman ◽  
...  
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Cell Lines ◽  
Caspase 3 ◽  
Mononuclear Cells ◽  
P53 Protein ◽  
Lymphocytic Leukemia ◽  
Vitro Activity ◽  
Interleukin 4 ◽  
In Vitro Activity

Abstract Flavopiridol has been reported to induce apoptosis in lymphoid cell lines via downregulation of bcl-2. The in vitro activity of flavopiridol against human chronic lymphocytic leukemia (CLL) cells and potential mechanisms of action for inducing cytotoxicity were studied. The in vitro viability of mononuclear cells from CLL patients (n = 11) was reduced by 50% at 4 hours, 24 hours, and 4 days at a flavopiridol concentration of 1.15 μmol/L (95% confidence interval [CI] ±0.31), 0.18 μmol/L (95% CI ±0.04), and 0.16 μmol/L (95% CI ±0.04), respectively. Loss of viability in human CLL cells correlated with early induction of apoptosis. Exposure of CLL cells to 0.18 μmol/L of flavopiridol resulted in both decreased expression of p53 protein and cleavage of the caspase-3 zymogen 32-kD protein with the appearance of its 20-kD subunit. Contrasting observations of others in tumor cell lines, flavopiridol cytotoxicity in CLL cells did not correlate with changes in bcl-2 protein expression alterations. We evaluated flavopiridol’s dependence on intact p53 by exposing splenocytes from wild-type (p53+/+) and p53 null (p53−/−) mice that demonstrated no preferential cytotoxicity as compared with a marked differential with F-ara-a and radiation. Incubation of CLL cells with antiapoptotic cytokine interleukin-4 (IL-4) did not alter the LC50 of flavopiridol, as compared with a marked elevation noted with F-ara-a in the majority of patients tested. These data demonstrate that flavopiridol has significant in vitro activity against human CLL cells through activation of caspase-3, which appears to occur independently of bcl-2 modulation, the presence of IL-4, or p53 status. Such findings strongly support the early introduction of flavopiridol into clinical trials for patients with B-CLL.

In Vitro Activity of Essential Oils from San Luis-Argentina AgainstAscosphaera apis

2003 ◽  
Vol 15 (4) ◽  
pp. 282-285 ◽  
Author(s):  
Alejandro D. Dellacasa ◽  
Pedro N. Bailac ◽  
Marta I. Ponzi ◽  
Sergio R. Ruffinengo ◽  
Martín J. Eguaras
Keyword(s):  
Essential Oils ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
San Luis

scholarly journals Selected ethno-medicinal plants from Kenya with in vitro activity against major African livestock pathogens belonging to the “ Mycoplasma mycoides cluster”

2016 ◽  
Vol 192 ◽  
pp. 524-534 ◽  
Author(s):  
Francisca Kama-Kama ◽  
Jacob Midiwo ◽  
Joseph Nganga ◽  
Naomi Maina ◽  
Elise Schiek ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Mycoplasma Mycoides

Sensitivity to Amphotericin B of in vitro Established Cell Lines

Nature ◽  
1974 ◽  
Vol 247 (5441) ◽  
pp. 466-469 ◽  
Author(s):  
P. AMATI ◽  
C. LAGO
Keyword(s):  
Amphotericin B ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Hypoxia affects in vitro growth of newly established cell lines from patients with malignant pleural mesothelioma

2013 ◽  
Vol 34 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Houman Goudarzi ◽  
Yasuhiro Hida ◽  
Hiroko Takano ◽  
Hiroki Teramae ◽  
Hisashi Iizasa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Malignant Pleural Mesothelioma ◽  
Pleural Mesothelioma ◽  
In Vitro Growth ◽  
Established Cell Lines ◽  
Established Cell

In vitro activity of antifungals in combination with essential oils against the oomycete Pythium insidiosum

2016 ◽  
Vol 121 (4) ◽  
pp. 998-1003 ◽  
Author(s):  
J.S. Valente ◽  
A.O.S. Fonseca ◽  
L.B. Denardi ◽  
V.S. Dal Ben ◽  
F.S. Maia Filho ◽  
...  
Keyword(s):  
Essential Oils ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Pythium Insidiosum
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