EFFECT OF LIPOTROPIC AGENTS UPON THE NET SYNTHESIS OF CHOLESTEROL IN THE RAT

1960 ◽  
Vol 38 (1) ◽  
pp. 757-761
Author(s):  
Sailen Mookerjea ◽  
C. C. Lucas
Keyword(s):  
Choline Chloride ◽  
Young Male ◽  
Significant Degree ◽  
Male Rats ◽  
Vitamin B ◽  
Normal Range ◽  
Liver Lipids ◽  
Significant Difference

The content of cholesterol in the bodies of young male rats fed a basal hypolipotropic diet has been compared with that of other rats pair-fed on similar diets supplemented with choline chloride, methionine, or vitamin B12, respectively. Increased efficiency of utilization of the food and variable lipotropic effects were observed, but no significant difference was found in the total amounts of cholesterol at the end of the 3-week test periods. The lipotropic agents do not influence to any significant degree the net biosynthesis of cholesterol in rats fed this type of diet but they do affect the distribution, tending to keep the liver lipids, including cholesterol, in the normal range.

EFFECT OF LIPOTROPIC AGENTS UPON THE NET SYNTHESIS OF CHOLESTEROL IN THE RAT

1960 ◽  
Vol 38 (7) ◽  
pp. 757-761
Author(s):  
Sailen Mookerjea ◽  
C. C. Lucas
Keyword(s):  
Choline Chloride ◽  
Young Male ◽  
Significant Degree ◽  
Male Rats ◽  
Vitamin B ◽  
Normal Range ◽  
Liver Lipids ◽  
Significant Difference

The content of cholesterol in the bodies of young male rats fed a basal hypolipotropic diet has been compared with that of other rats pair-fed on similar diets supplemented with choline chloride, methionine, or vitamin B12, respectively. Increased efficiency of utilization of the food and variable lipotropic effects were observed, but no significant difference was found in the total amounts of cholesterol at the end of the 3-week test periods. The lipotropic agents do not influence to any significant degree the net biosynthesis of cholesterol in rats fed this type of diet but they do affect the distribution, tending to keep the liver lipids, including cholesterol, in the normal range.

IN VITRO STUDIES ON CARBOHYDRATE METABOLISM IN THE VITAMIN-B6-DEPRIVED RAT

1955 ◽  
Vol 33 (1) ◽  
pp. 562-567
Author(s):  
John R. Beaton
Keyword(s):  
Carbohydrate Metabolism ◽  
Vitamin B6 ◽  
Glucose Utilization ◽  
Female Rats ◽  
Male Rats ◽  
Vitamin B ◽  
Significant Difference ◽  
Aldolase Activity ◽  
Food Consumed

Following earlier studies on carbohydrate metabolism in the vitamin-B6-deprived rat, in vitro investigations have been carried out. In all cases, comparisons were made between tissues from vitamin-B6-deprived and pair-fed control animals so that differences in the amount of food consumed would not affect the interpretation of experimental results. No significant difference was found in glucose utilization by muscle nor in liver cytochrome oxidase activity. Liver aldolase activity was significantly decreased and the activity of plasma alkaline phosphatase was significantly increased in the vitamin-B6-deprived rats. In vitamin-B6-deprived female rats, but not male rats, liver catalase activity was significantly increased. These results are discussed in the light of earlier observations indicating disturbances in carbohydrate metabolism in the vitamin-B6-deprived rat.

scholarly journals Diltiazem did not induce gingival overgrowth in rats: a clinical, histological and histometric analysis

2005 ◽  
Vol 19 (3) ◽  
pp. 163-168 ◽  
Author(s):  
Fernanda de Oliveira Bello Corrêa ◽  
Gabriela Giro ◽  
Daniela Gonçalves ◽  
Luis Carlos Spolidorio ◽  
Silvana Regina Perez Orrico
Keyword(s):  
Body Weight ◽  
Treatment Duration ◽  
Young Male ◽  
Male Rats ◽  
Channel Blockers ◽  
Connective Tissues ◽  
Control Groups ◽  
Duration Of Treatment ◽  
Gingival Overgrowth ◽  
Significant Difference

The administration of calcium channel blockers has been associated with gingival overgrowth. However, there are few studies in humans or animals that evaluated the effect of diltiazem on gingival tissues. The present study assessed the influence of diltiazem, at different dosages and treatment duration, on gingival tissues of rats, using clinical, histological and histometric analyses. Eighty young male rats were separated into eight groups according to the dosage and duration of treatment. Rats were treated for 20 or 40 days with a daily subcutaneous injection of 5, 20 or 50 mg/kg of body weight of diltiazem. The results confirmed that diltiazem did not induce gingival overgrowth in rats. For all animals, the evaluation did not show gingival alterations regardless of the dosages and periods of treatment. The histometric analysis showed no significant change in the area of epithelium and connective tissues, although after 40 days of treatment a decrease in the area of connective tissue was observed, without statistically significant difference from control groups. Within the limits of this study, we suggest that diltiazem did not induce gingival overgrowth.

scholarly journals Reduced Bone and Body Mass in Young Male Rats Exposed to Lead

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Fellipe Augusto Tocchini de Figueiredo ◽  
Raquel Fernanda Gerlach ◽  
Márcia Andreia Mesquita Silva da Veiga ◽  
Flavio Venancio Nakadi ◽  
Junia Ramos ◽  
...  
Keyword(s):  
Whole Blood ◽  
Young Male ◽  
Blood Lead ◽  
Male Rats ◽  
Control Groups ◽  
Growing Rats ◽  
Bone Lead ◽  
Exposure Biomarker ◽  
Significant Difference ◽  
Gf Aas

The aim of this study was to see whether there would be differences in whole blood versus tibia lead concentrations over time in growing rats prenatally. Lead was given in the drinking water at 30 mg/L from the time the dams were pregnant until offspring was 28- or 60-day-old. Concentrations of lead were measured in whole blood and in tibia after 28 (28D) and 60 days (60D) in control (C) and in lead-exposed animals (Pb). Lead measurements were made by GF-AAS. There was no significant difference (P>0.05) in the concentration of whole blood lead between Pb-28D (8.0±1.1 μg/dL) and Pb-60D (7.2±0.89 μg/dL), while both significantly varied (P<0.01) from controls (0.2 μg/dL). Bone lead concentrations significantly varied between the Pb-28D (8.02±1.12 μg/g) and the Pb-60D (43.3±13.26 μg/g) lead-exposed groups (P<0.01), while those exposed groups were also significantly higher (P<0.0001) than the 28D and 60D control groups (Pb < 1 μg/g). The Pb-60D group showed a 25% decrease in tibia mass as compared to the respective control. The five times higher amount of lead found in the bone of older animals (Pb-60D versus Pb-28D), which reinforces the importance of using bone lead as an exposure biomarker.

Cardiac function, microvascular structure, and capillary hematocrit in hearts of polycythemic rats

2001 ◽  
Vol 281 (6) ◽  
pp. H2425-H2431 ◽  
Author(s):  
Karel Rakusan ◽  
N. Cicutti ◽  
F. Kolar
Keyword(s):  
Young Male ◽  
Experimental Models ◽  
Left Ventricular ◽  
Male Rats ◽  
Cell Distribution ◽  
Normal Range ◽  
Functional Reserve ◽  
Geometric Conditions ◽  
Capillary Angiogenesis ◽  
Induced Changes

The effect of polycythemia on the coronary microcirculation was studied in young male rats. Two experimental models of polycythemia were employed: cobalt-induced polycythemia, which mimics hypoxia-induced changes, and erythropoietin-induced polycythemia, which circumvents these changes. In both models, baseline left ventricular function was normal, whereas maximal systolic and developed pressures were decreased. In cobalt-treated rats the left ventricular functional reserve was also compromised. Morphometric analysis of the left ventricle confirmed previously described improved geometric conditions for oxygen supply at the distal portions of capillaries (smaller domain areas and shorter capillary segments). In cobalt-treated but not in erythropoietin-treated rats, increased capillary angiogenesis was also detected. In the hearts from rats with both types of polycythemia, a small but significant increase in the formation of arterioles was found. Capillary linear hematocrit was within the normal range in both types of polycythemia despite sizeable increases in systemic hematocrit. Significant differences in red blood cell distribution within capillaries were found between proximal and distal portions in all experimental groups.

IN VITRO STUDIES ON CARBOHYDRATE METABOLISM IN THE VITAMIN-B6-DEPRIVED RAT

1955 ◽  
Vol 33 (4) ◽  
pp. 562-567
Author(s):  
John R. Beaton
Keyword(s):  
Carbohydrate Metabolism ◽  
Vitamin B6 ◽  
Glucose Utilization ◽  
Female Rats ◽  
Male Rats ◽  
Vitamin B ◽  
Significant Difference ◽  
Aldolase Activity ◽  
Food Consumed

Following earlier studies on carbohydrate metabolism in the vitamin-B6-deprived rat, in vitro investigations have been carried out. In all cases, comparisons were made between tissues from vitamin-B6-deprived and pair-fed control animals so that differences in the amount of food consumed would not affect the interpretation of experimental results. No significant difference was found in glucose utilization by muscle nor in liver cytochrome oxidase activity. Liver aldolase activity was significantly decreased and the activity of plasma alkaline phosphatase was significantly increased in the vitamin-B6-deprived rats. In vitamin-B6-deprived female rats, but not male rats, liver catalase activity was significantly increased. These results are discussed in the light of earlier observations indicating disturbances in carbohydrate metabolism in the vitamin-B6-deprived rat.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

scholarly journals Inter-Limb Jump Asymmetries and Their Association with Sport-Specific Performance in Young Male and Female Swimmers

2021 ◽  
Vol 18 (14) ◽  
pp. 7324
Author(s):  
Max I. Phukan ◽  
Rohit K. Thapa ◽  
Gopal Kumar ◽  
Chris Bishop ◽  
Helmi Chaabene ◽  
...  
Keyword(s):  
National Level ◽  
Young Male ◽  
Front Crawl ◽  
Male And Female ◽  
Standing Long Jump ◽  
Significant Difference ◽  
Specific Performance ◽  
Long Jump ◽  
Males And Females ◽  
Level Of Agreement

This study aimed to examine inter-limb jump asymmetries and their association with sport-specific performance in young swimmers. Thirty-eight (male, n = 19; female, n = 19) regional/national level young swimmers (age: 12.3 ± 1.2 years; height: 159.6 ± 8.2 cm; body mass: 52.5 ± 9.2 kg) participated in this study. Inter-limb asymmetries were assessed for single-leg countermovement jump (SLCMJ) and single-leg standing long jump (SLSLJ). Sport-specific performance was evaluated using front crawl (i.e., 50 m and 25 m) and front crawl kick (i.e., 50 m and 25 m). The kappa coefficient revealed a “slight” level of agreement (Κ = 0.156, 0.184, and 0.197 for female, male, and all, respectively) between the direction of asymmetry for SLCMJ and SLSLJ, indicating that asymmetries rarely favored the same limb during both tests. A paired sample t-test showed a significant difference (p = 0.025) between asymmetry scores obtained in SLCMJ and SLSLJ. No significant difference was found in asymmetry scores between males and females (p = 0.099 to 0.977). Additionally, no association between asymmetry scores and sport-specific performance was observed (p > 0.05). Our findings highlight the independent nature of inter-limb asymmetries derived from SLCMJ and SLSLJ among young male and female swimmers. Further, our results suggest no association between jumping asymmetries and sport-specific performance.

Sign in / Sign up

Export Citation Format

Share Document