EFFECT OF X IRRADIATION ON THE METABOLISM OF C14-GLUCOSE AND C14-FRUCTOSE IN RAT LIVER SLICES

1959 ◽  
Vol 37 (1) ◽  
pp. 787-792
Author(s):  
P. V. Vittorio ◽  
W. P. Spence ◽  
M. J. Johnston
Keyword(s):  
Carbon Dioxide ◽  
Ethanolic Extract ◽  
Total Radioactivity ◽  
Whole Body ◽  
Partition Chromatography ◽  
Liver Slices ◽  
Ethanolic Extracts ◽  
X Irradiation ◽  
Origin Area ◽  
Control Samples

The utilization of C14-glucose and C14-fructose in liver slices from normal rats and from rats exposed to 1000 r of whole-body X irradiation was studied. Liver slices prepared from normal rats were incubated with C14-glucose or C14-fructose in equivalent amounts and the incorporation of C14 into carbon dioxide, glycogen, and an ethanolic extract was determined. After the rats had been fasted 4 or 24 hours the amount of C14 incorporated into glycogen and carbon dioxide from C14-fructose was greater than that incorporated from C14-glucose but the total radioactivity in the ethanolic extracts was approximately the same for both hexoses. When liver slices prepared from normal and X-irradiated rats were incubated with C14-glucose or C14-fructose 4 or 24 hours after irradiation, the samples obtained from irradiated rats incorporated a greater amount of C14 into carbon dioxide, glycogen, and the ethanolic extract, with the exception of the 24-hour samples incubated in the presence of labelled glucose. In the latter instance incorporation into carbon dioxide fell below the normal value. The total C14 recovery from the three fractions was always higher in the X-irradiated samples than in the corresponding control samples. Further examination of the ethanolic extracts (amino acids, lactic acid, and origin area material) separated by paper partition chromatography revealed additional differences between the samples of liver from normal and X-irradiated rats in their ability to incorporate C14 from either labelled hexose. These differences were apparent in samples incubated either 4 or 24 hours after X irradiation of the animals.

EFFECT OF X IRRADIATION ON THE METABOLISM OF C14-GLUCOSE AND C14-FRUCTOSE IN RAT LIVER SLICES

1959 ◽  
Vol 37 (6) ◽  
pp. 787-792 ◽  
Author(s):  
P. V. Vittorio ◽  
W. P. Spence ◽  
M. J. Johnston
Keyword(s):  
Carbon Dioxide ◽  
Ethanolic Extract ◽  
Total Radioactivity ◽  
Whole Body ◽  
Partition Chromatography ◽  
Liver Slices ◽  
Ethanolic Extracts ◽  
X Irradiation ◽  
Origin Area ◽  
Control Samples

The utilization of C14-glucose and C14-fructose in liver slices from normal rats and from rats exposed to 1000 r of whole-body X irradiation was studied. Liver slices prepared from normal rats were incubated with C14-glucose or C14-fructose in equivalent amounts and the incorporation of C14 into carbon dioxide, glycogen, and an ethanolic extract was determined. After the rats had been fasted 4 or 24 hours the amount of C14 incorporated into glycogen and carbon dioxide from C14-fructose was greater than that incorporated from C14-glucose but the total radioactivity in the ethanolic extracts was approximately the same for both hexoses. When liver slices prepared from normal and X-irradiated rats were incubated with C14-glucose or C14-fructose 4 or 24 hours after irradiation, the samples obtained from irradiated rats incorporated a greater amount of C14 into carbon dioxide, glycogen, and the ethanolic extract, with the exception of the 24-hour samples incubated in the presence of labelled glucose. In the latter instance incorporation into carbon dioxide fell below the normal value. The total C14 recovery from the three fractions was always higher in the X-irradiated samples than in the corresponding control samples. Further examination of the ethanolic extracts (amino acids, lactic acid, and origin area material) separated by paper partition chromatography revealed additional differences between the samples of liver from normal and X-irradiated rats in their ability to incorporate C14 from either labelled hexose. These differences were apparent in samples incubated either 4 or 24 hours after X irradiation of the animals.

scholarly journals Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii)

2016 ◽  
Vol 16 (1) ◽  
pp. 9
Author(s):  
Ummu Balqis ◽  
Darmawi Darmawi ◽  
Maryam Maryam ◽  
Muslina Muslina ◽  
Abdullah Hamzah ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
The Body ◽  
Whole Body ◽  
Ascaridia Galli ◽  
Fourth Group ◽  
The Third ◽  
Ethanolic Extracts ◽  
Aqueous Ethanolic Extract ◽  
Normal Controls

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.  (Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii) ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.

THE EFFECT OF 2-AMINOETHYLISOTHIOURONIUM (AET) ON THE RESPIRATION OF MOUSE LIVER AND SPLEEN SLICES

1960 ◽  
Vol 38 (1) ◽  
pp. 819-822
Author(s):  
P. V. Vittorio ◽  
Wilma P. Spence
Keyword(s):  
Mouse Liver ◽  
Whole Body ◽  
Depressing Effect ◽  
Bicarbonate Buffer ◽  
Liver Slices ◽  
X Irradiation

The effect of AET injected in vivo on the respiration of liver and spleen slices from non-irradiated and X-irradiated mice was studied. The mice were killed 4 or 24 hours after the injection of a protective dose of AET and the livers and spleens were removed, sliced, and incubated for 3 hours in Krebs–Henseleit bicarbonate buffer containing uniformly labelled C14 glucose. In both non-irradiated and X-irradiated mice, initially, AET depressed the respiration of C14O2 from incubated liver slices but this depressing effect appeared to be overcome with time. Whole body X irradiation increased the total C14O2 respired by liver slices from mice injected with saline or AET prior to X irradiation and killed 4 or 24 hours after X irradiation. Whole body X irradiation decreased the total C14O2 respired by the spleen slices and the 24-hour samples showed the greatest decrease. In both the non-irradiated and X-irradiated mice AET did not appear to affect the total C14O2 respired by spleen slices.

THE EFFECT OF 2-AMINOETHYLISOTHIOURONIUM (AET) ON THE RESPIRATION OF MOUSE LIVER AND SPLEEN SLICES

1960 ◽  
Vol 38 (8) ◽  
pp. 819-822
Author(s):  
P. V. Vittorio ◽  
Wilma P. Spence
Keyword(s):  
Mouse Liver ◽  
Whole Body ◽  
Depressing Effect ◽  
Bicarbonate Buffer ◽  
Liver Slices ◽  
X Irradiation

The effect of AET injected in vivo on the respiration of liver and spleen slices from non-irradiated and X-irradiated mice was studied. The mice were killed 4 or 24 hours after the injection of a protective dose of AET and the livers and spleens were removed, sliced, and incubated for 3 hours in Krebs–Henseleit bicarbonate buffer containing uniformly labelled C14 glucose. In both non-irradiated and X-irradiated mice, initially, AET depressed the respiration of C14O2 from incubated liver slices but this depressing effect appeared to be overcome with time. Whole body X irradiation increased the total C14O2 respired by liver slices from mice injected with saline or AET prior to X irradiation and killed 4 or 24 hours after X irradiation. Whole body X irradiation decreased the total C14O2 respired by the spleen slices and the 24-hour samples showed the greatest decrease. In both the non-irradiated and X-irradiated mice AET did not appear to affect the total C14O2 respired by spleen slices.

scholarly journals Anticancer effects of the combined Thai noni juice ethanolic extracts and 5-fluorouracil against cholangiocarcinoma cells in vitro and in vivo

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jeerati Prompipak ◽  
Thanaset Senawong ◽  
Banchob Sripa ◽  
Albert J. Ketterman ◽  
Suppawit Utaiwat ◽  
...  
Keyword(s):  
Nude Mice ◽  
Combination Treatment ◽  
Synergistic Effects ◽  
Single Agent ◽  
Xenograft Model ◽  
Ethanolic Extract ◽  
Model Combination ◽  
Mouse Xenograft Model ◽  
Ethanolic Extracts

AbstractApplication of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy of 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim of this study was to evaluate the combination effects of 5-FU and two ethanolic extracts of Thai noni juice (TNJ) products on CCA cell lines and nude mice xenografts. The results of antiproliferative assay showed the combination treatment of 5-FU and each TNJ ethanolic extract exerted more cytotoxicity on CCA cells than either single agent treatment. Synergistic effects of drug combinations can enable the dose reduction of 5-FU. The mechanism underlying a combination treatment was apoptosis induction through an activation of p53 and Bax proteins. In the nude mouse xenograft model, combination treatments of 5-FU with each TNJ ethanolic extract suppressed the growth of CCA cells implanted mice more than single agent treatments with no effects on mouse body weight, kidney, and spleen. Moreover, low doses of TNJ ethanolic extracts reduced the hepatotoxicity of 5-FU in nude mice. Taken together, these data suggested that the ethanolic extracts of TNJ products can enhance the anti-CCA effect and reduce toxicity of 5-FU.

scholarly journals Betel (Piper betle L.) leaf ethanolic extracts dechlorophyllized using different methods: antioxidant and antibacterial activities, and application for shelf-life extension of Nile tilapia (Oreochromis niloticus) fillets

RSC Advances ◽  
2021 ◽  
Vol 11 (29) ◽  
pp. 17630-17641
Author(s):  
Mohamed Tagrida ◽  
Soottawat Benjakul
Keyword(s):  
Shelf Life ◽  
Nile Tilapia ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Life Extension ◽  
Piper Betle ◽  
Refrigerated Storage ◽  
Betel Leaf ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Ethanolic Extracts

Betel leaf ethanolic extract dechlorophyllized using sedimentation (BLEE-SED) exhibited enhanced antioxidant and antibacterial activities. BLEE-SED could extend the shelf-life of Nile tilapia fillets during refrigerated storage (4 °C) up to 9 days.

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