ON THE INDUCED SYNTHESIS OF AN ENZYME REQUIRED FOR BIOSYNTHESIS OF AN ESSENTIAL METABOLITE: INDUCED KYNURENINASE SYNTHESIS IN NEUROSPORA CRASSA

1959 ◽  
Vol 37 (1) ◽  
pp. 741-750
Author(s):  
S. D. Wainwright ◽  
D. M. Bonner
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting ◽  
Enzyme Formation

Brief incubation of washed mycelial pads of Neurospora crassa with L-tryptophan or L-kynurenine caused a marked and rapid increase in kynureninase activity. Some of the factors affecting the extent of enzyme formation were studied. Tryptophan was almost as effective an inducer of kynureninase formation as kynurenine. The significance of induced kynureninase formation as a regulator of niacin biosynthesis is discussed.

ON THE INDUCED SYNTHESIS OF AN ENZYME REQUIRED FOR BIOSYNTHESIS OF AN ESSENTIAL METABOLITE: INDUCED KYNURENINASE SYNTHESIS IN NEUROSPORA CRASSA

1959 ◽  
Vol 37 (6) ◽  
pp. 741-750 ◽  
Author(s):  
S. D. Wainwright ◽  
D. M. Bonner
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting ◽  
Enzyme Formation

Brief incubation of washed mycelial pads of Neurospora crassa with L-tryptophan or L-kynurenine caused a marked and rapid increase in kynureninase activity. Some of the factors affecting the extent of enzyme formation were studied. Tryptophan was almost as effective an inducer of kynureninase formation as kynurenine. The significance of induced kynureninase formation as a regulator of niacin biosynthesis is discussed.

scholarly journals Factors affecting DNA uptake by Neurospora crassa

1977 ◽  
Vol 24 (1) ◽  
Author(s):  
J. Aradi ◽  
M. Schablik ◽  
A. Zsindely ◽  
A. Kiss ◽  
M. Szabolcs ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Dna Uptake ◽  
Factors Affecting

scholarly journals Mendelian and Non-Mendelian Factors Affecting the Cytochrome System in Neurospora Crassa

1953 ◽  
Vol 39 (7) ◽  
pp. 606-613 ◽  
Author(s):  
M. B. Mitchell ◽  
H. K. Mitchell ◽  
A. Tissieres
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting

HETEROKARYOTIC TRANSMISSION OF FACTORS AFFECTING RECOMBINATION IN NEUROSPORA CRASSA

1968 ◽  
Vol 10 (2) ◽  
pp. 248-252 ◽  
Author(s):  
A. J. F. Griffiths
Keyword(s):  
Neurospora Crassa ◽  
Gene Products ◽  
Factors Affecting

Complementation of recombination-inhibiting gene products has been demonstrated in crosses using the heterokaryon as conidial parent. Ways in which this complementation might be effected are examined in the light of corollary experiments.

Progress In The Practical Application Of Automated Image Analysis To Tem Negatives

1977 ◽  
Vol 35 ◽  
pp. 214-217
Author(s):  
F. A. Heckman ◽  
E. Redman ◽  
J.E. Connolly
Keyword(s):  
Image Analysis ◽  
Image Quality ◽  
Exposure Time ◽  
Image Contrast ◽  
Automated Image Analysis ◽  
Practical Application ◽  
Factors Affecting ◽  
High Image Quality ◽  
Qualitative Evidence ◽  
Comprehensive Study

In our initial publication on this subject1) we reported results demonstrating that contrast is the most important factor in producing the high image quality required for reliable image analysis. We also listed the factors which enhance contrast in order of the experimentally determined magnitude of their effect. The two most powerful factors affecting image contrast attainable with sheet film are beam intensity and KV. At that time we had only qualitative evidence for the ranking of enhancing factors. Later we carried out the densitometric measurements which led to the results outlined below.Meaningful evaluations of the cause-effect relationships among the considerable number of variables in preparing EM negatives depend on doing things in a systematic way, varying only one parameter at a time. Unless otherwise noted, we adhered to the following procedure evolved during our comprehensive study:Philips EM-300; 30μ objective aperature; magnification 7000- 12000X, exposure time 1 second, anti-contamination device operating.

Ultrastructure and Morphology of the Neurospora Crassa Cell Wall Mutants, Slime And Slime X, Using Tem and Sem

1976 ◽  
Vol 34 ◽  
pp. 54-55
Author(s):  
Karen S. Howard ◽  
H. D. Braymer ◽  
M. D. Socolofsky ◽  
S. A. Milligan
Keyword(s):  
Cell Wall ◽  
Neurospora Crassa ◽  
Wild Type ◽  
Morphological Comparison ◽  
Small Areas ◽  
Solid Media ◽  
Thin Sectioning ◽  
X Cells ◽  
Mutant Cells ◽  
Isolated Cell

The recently isolated cell wall mutant slime X of Neurospora crassa was prepared for ultrastructural and morphological comparison with the cell wall mutant slime. The purpose of this article is to discuss the methods of preparation for TEM and SEM observations, as well as to make a preliminary comparison of the two mutants.TEM: Cells of the slime mutant were prepared for thin sectioning by the method of Bigger, et al. Slime X cells were prepared in the same manner with the following two exceptions: the cells were embedded in 3% agar prior to fixation and the buffered solutions contained 5% sucrose throughout the procedure.SEM: Two methods were used to prepare mutant and wild type Neurospora for the SEM. First, single colonies of mutant cells and small areas of wild type hyphae were cut from solid media and fixed with OSO4 vapors similar to the procedure used by Harris, et al. with one alteration. The cell-containing agar blocks were dehydrated by immersion in 2,2-dimethoxypropane (DMP).

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