HETEROKARYOTIC TRANSMISSION OF FACTORS AFFECTING RECOMBINATION IN NEUROSPORA CRASSA

1968 ◽  
Vol 10 (2) ◽  
pp. 248-252 ◽  
Author(s):  
A. J. F. Griffiths
Keyword(s):  
Neurospora Crassa ◽  
Gene Products ◽  
Factors Affecting

Complementation of recombination-inhibiting gene products has been demonstrated in crosses using the heterokaryon as conidial parent. Ways in which this complementation might be effected are examined in the light of corollary experiments.

ON THE INDUCED SYNTHESIS OF AN ENZYME REQUIRED FOR BIOSYNTHESIS OF AN ESSENTIAL METABOLITE: INDUCED KYNURENINASE SYNTHESIS IN NEUROSPORA CRASSA

1959 ◽  
Vol 37 (1) ◽  
pp. 741-750
Author(s):  
S. D. Wainwright ◽  
D. M. Bonner
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting ◽  
Enzyme Formation

Brief incubation of washed mycelial pads of Neurospora crassa with L-tryptophan or L-kynurenine caused a marked and rapid increase in kynureninase activity. Some of the factors affecting the extent of enzyme formation were studied. Tryptophan was almost as effective an inducer of kynureninase formation as kynurenine. The significance of induced kynureninase formation as a regulator of niacin biosynthesis is discussed.

ON THE INDUCED SYNTHESIS OF AN ENZYME REQUIRED FOR BIOSYNTHESIS OF AN ESSENTIAL METABOLITE: INDUCED KYNURENINASE SYNTHESIS IN NEUROSPORA CRASSA

1959 ◽  
Vol 37 (6) ◽  
pp. 741-750 ◽  
Author(s):  
S. D. Wainwright ◽  
D. M. Bonner
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting ◽  
Enzyme Formation

Brief incubation of washed mycelial pads of Neurospora crassa with L-tryptophan or L-kynurenine caused a marked and rapid increase in kynureninase activity. Some of the factors affecting the extent of enzyme formation were studied. Tryptophan was almost as effective an inducer of kynureninase formation as kynurenine. The significance of induced kynureninase formation as a regulator of niacin biosynthesis is discussed.

scholarly journals Isolation of a gene that down-regulates nitrate assimilation and influences another regulatory gene in the same system.

1989 ◽  
Vol 9 (9) ◽  
pp. 4113-4117 ◽  
Author(s):  
G J Sorger ◽  
D Brown ◽  
M Farzannejad ◽  
A Guerra ◽  
M Jonathan ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Gene Product ◽  
Regulatory Gene ◽  
Nitrate Assimilation ◽  
Gene Products ◽  
Specific Pathway

Glutamine is the preferred source of nitrogen of Neurospora crassa. In its presence and that of the gene product of MS5 (nmr-1), the fungus represses the assimilation of less preferred forms of nitrogen, such as nitrate. In the absence of glutamine and the presence of the product of gene nit-2, less preferred forms of nitrogen are assimilated as long as a specific pathway for their assimilation is induced. We report here the isolation, from a cosmid bank, of a gene that complements MS5 and can also complement nit-2. We speculate that this result suggests an interaction between the MS5 and nit-2 gene products and that this is important in the regulation of nitrate assimilation.

scholarly journals Factors affecting DNA uptake by Neurospora crassa

1977 ◽  
Vol 24 (1) ◽  
Author(s):  
J. Aradi ◽  
M. Schablik ◽  
A. Zsindely ◽  
A. Kiss ◽  
M. Szabolcs ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Dna Uptake ◽  
Factors Affecting

scholarly journals Mendelian and Non-Mendelian Factors Affecting the Cytochrome System in Neurospora Crassa

1953 ◽  
Vol 39 (7) ◽  
pp. 606-613 ◽  
Author(s):  
M. B. Mitchell ◽  
H. K. Mitchell ◽  
A. Tissieres
Keyword(s):  
Neurospora Crassa ◽  
Factors Affecting

scholarly journals COMPLEMENTATION ANALYSIS OF METABOLITE-RESISTANT MUTATIONS WITH FORCED HETEROKARYONS OF NEUROSPORA CRASSA

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 581-593
Author(s):  
W M Thwaites ◽  
F K Knauert ◽  
S S Carney
Keyword(s):  
Positive Result ◽  
Neurospora Crassa ◽  
Mutant Strain ◽  
Uptake Rate ◽  
Double Mutant ◽  
Complementation Test ◽  
Gene Products ◽  
Double Mutant Strain ◽  
Common Precursor ◽  
Resistant Mutants

ABSTRACT The double mutant strain pyr-3  arg-12s is a prototroph because a common precursor of arginine and pyrimidine is supplied by the arginine pathway. Growth of this strain is inhibited by exogenous citrulline or arginine. Citrulline-resistant mutants of this strain were selected, and they resulted from modifier mutations at other loci. Forced heterokaryons were used to study complementation among these modifiers. Since the complementation test requires the scoring of non-growth as the positive result, there was concern that variations in nuclear ratios could give erroneous results. This possibility does not seem significant, since groups of mutants established by complementation correspond with groups established by physiological, enzymatic, and recombinational measurements.—The technique has revealed that the most frequently mutated loci are arg-1 and what is probably un-3. Arg-1 mutations affect the conversion of citrulline to argininosuccinate, while un-3 mutations reduce the citrulline uptake rate. Since most of these mutations are of the intracistronic complementing type, a complementation map was constructed for most of the affected loci. The high proportion of complementors in each map can be explained by assuming that partially functioning gene products are more likely to complement with each other than are those which are nonfunctional.

scholarly journals A Role of Calcium Signaling Genes in Heterokaryon Incompatibility in Neurospora crassa

2015 ◽  
Vol 3 (4) ◽  
pp. 668-679
Author(s):  
Ravi Gedela ◽  
Ranjan Tamuli
Keyword(s):  
Neurospora Crassa ◽  
Mating Type ◽  
Gene Deletion ◽  
Deletion Mutants ◽  
Gene Products ◽  
Wild Type ◽  
Heterokaryon Incompatibility ◽  
Knockout Mutants ◽  
Wild Type Control

We have studied the Ca2+-signaling knockout mutants for their role in mating-type-associated heterokaryon incompatibility in Neurospora crassa.  The found results showed on heterokaryons homokaryosis for DNCU05225, DNCU06366, DNCU06650, DNCU07075, and ∆NCU07966 Ca2+-signaling knockout mutants (Neurospora crassa unit number, NCU) displayed heterokaryon het compatibility; however heterokaryons heterokaryosis for DNCU05225, DNCU063665, DNCU06650, DNCU07075, and ∆NCU07966 mutants displayed het incompatibility like the wild-type control.  In addition to that Two Ca2+-signaling knockout mutants DNCU02283, and DNCU09655 were tested for mating-type-associated heterokaryon incompatibility; these results showed, heterokaryons homokaryosis and heterokaryons heterokaryosis for DNCU02283, DNCU09655 mutants displayed het incompatibility.  Cell death and hyphal compartmentation due to mating type associated incompatibility were confirmed by uptake of vital dye Evan’s blue.  Thus, these results of NCU05225, NCU06366, NCU06650, NCU07075, and NCU07966 Ca2+-signaling gene products could play a role in mating-type-associated heterokaryon incompatibility in N. crassa.  In this article, we are reporting initially screened Ca2+-signaling gene deletion mutants of these five acts as recessive suppressors of mating type associated vegetative incompatibility in N. crassa.Int J Appl Sci Biotechnol, Vol 3(4): 668-679

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