ADSORPTION OF ORANGE II BY CYTOCHROME c AND RIBONUCLEASE

1954 ◽  
Vol 32 (1) ◽  
pp. 184-188
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Cytochrome C ◽  
Adsorption Isotherms ◽  
Acetate Buffer ◽  
Orange Ii

The adsorption isotherms of Orange II on cytochrome c and ribonuclease at pH 5.5 in 0.05 M acetate buffer are sigmoid. They may be interpreted by a previously described theory of interacting hydration effects. Adsorption of methyl orange or sodium flavianate by either protein was negligible.

ADSORPTION OF ORANGE II BY CYTOCHROME c AND RIBONUCLEASE

1954 ◽  
Vol 32 (3) ◽  
pp. 184-188 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Cytochrome C ◽  
Adsorption Isotherms ◽  
Acetate Buffer ◽  
Orange Ii

The adsorption isotherms of Orange II on cytochrome c and ribonuclease at pH 5.5 in 0.05 M acetate buffer are sigmoid. They may be interpreted by a previously described theory of interacting hydration effects. Adsorption of methyl orange or sodium flavianate by either protein was negligible.

A NOTE ON THE STOIGHIOMETRY OF ADSORPTION OF ANIONS BY LYSOZYME

1955 ◽  
Vol 33 (4) ◽  
pp. 651-653 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Picric Acid ◽  
Sulphonic Acid ◽  
Acetate Buffer ◽  
Orange Ii ◽  
Necessary And Sufficient

Ten adsorbed anions of monovalent methyl orange, Orange II, or picric acid are necessary and sufficient to precipitate one lysozyme molecule at pH 5.5 in 0.05 M acetate buffer at 32°, whereas only five of divalent 2,4-dinitro-1-naphthol-7-sulphonic acid (flavianic acid) are required. These results are consistent with a hypothesis of interacting hydration effects.

A NOTE ON THE STOIGHIOMETRY OF ADSORPTION OF ANIONS BY LYSOZYME

1955 ◽  
Vol 33 (1) ◽  
pp. 651-653
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Picric Acid ◽  
Sulphonic Acid ◽  
Acetate Buffer ◽  
Orange Ii ◽  
Necessary And Sufficient

Ten adsorbed anions of monovalent methyl orange, Orange II, or picric acid are necessary and sufficient to precipitate one lysozyme molecule at pH 5.5 in 0.05 M acetate buffer at 32°, whereas only five of divalent 2,4-dinitro-1-naphthol-7-sulphonic acid (flavianic acid) are required. These results are consistent with a hypothesis of interacting hydration effects.

Polycations and Their Complexes

1977 ◽  
Vol 47 (8) ◽  
pp. 530-534 ◽  
Author(s):  
B. Courtney ◽  
J. B. Lawton
Keyword(s):  
Free Energy ◽  
Methyl Orange ◽  
Diffuse Reflectance ◽  
Color Change ◽  
Reflectance Spectra ◽  
Orange Ii ◽  
Alternative Treatments ◽  
Anionic Dyes ◽  
Diffuse Reflectance Spectra ◽  
Diethylaminoethyl Cellulose

Free-energy changes have been determined for the binding of the anionic dyes Orange II and Methyl Orange to diethylaminoethyl cellulose and triethylaminoethyl cellulose. These polycations resemble wool and other natural polycations in some respects. It is shown, however, that the Gilbert-Rideal theory is not satisfactory for cellulosic polycations, and alternative treatments are given. It was found that the order of binding of some simple anions was I- > Br- > Cl-. The sulphate ion behaved anomalously and appeared to be sensitive to changes in the intersite distance. Diffuse reflectance spectra showed evidence of a metachromatic color change in the spectrum of the bound dyes.

THE BINDING OF ANIONS BY LYSOZYME, CALF THYMUS HISTONE SULPHATE, AND PROTAMINE SULPHATE

1952 ◽  
Vol 30 (4) ◽  
pp. 320-331 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Protein Binding ◽  
Temperature Coefficient ◽  
Adsorption Isotherms ◽  
Large Series ◽  
Orange Ii ◽  
General Process ◽  
Protamine Sulphate ◽  
Calf Thymus ◽  
Pass Through ◽  
Positively Charged

The adsorption of a large series of anions by positively charged lysozyme, calf thymus histone sulphate, and protamine sulphate has been studied by dialysis equilibrium. One group of anions was not adsorbed by lysozyme while the adsorption isotherms for Orange I, Orange II, and 2, 4-dinitro-1-naphthol-7-sulphonic acid were S-shaped. These anomalous isotherms, which were also obtained with histone sulphate and protamine sulphate, have an appreciable temperature coefficient at intermediate dye concentrations. It is emphasized that this type of adsorption is inconsistent with the exclusively electrostatic view of protein binding of anions. It is also shown that −ΔF, −ΔH, and ΔS per mole of anion bound must pass through a maximum for intermediate free anion concentrations in such systems. The lack of binding of some anions and the anomalous isotherms for others is interpreted in terms of a solvation sheath about the charged protein. Their significance for the general process of binding of ions by proteins is discussed.

Synthesis of Polythiophene Nanoparticles by Microemulsion Polymerization for Photocatalysis

2011 ◽  
Vol 399-401 ◽  
pp. 1312-1319 ◽  
Author(s):  
Yang Cheng ◽  
Jun Wang ◽  
Yun Cheng Bao ◽  
Ya Li Ma ◽  
Guang Hui Wang
Keyword(s):  
Methyl Orange ◽  
Surface Area ◽  
Uv Irradiation ◽  
Conjugated Polymer ◽  
Organic Dyes ◽  
Orange Ii ◽  
Microemulsion Polymerization ◽  
Before And After ◽  
Photocatalytic Reactions ◽  
20 Nm

Abstract. Polythiophene nanoparticles prepared by W/O microemulsion method were found to be photocatalytically active for degradation of Orange II dye and methyl Orange. During the photodegradation of Orange II and Methyl Orange as organic dyes in UV/H2O2and UV irradiation systems using polythiophene nanoparticles as photocatalyst, it is exhibiting significant photocatalytic activity towards to double dyes in UV and UV/H2O2system. the diameter of spherical polymer nanoparticles is 100±20 nm which was characterized by TEM with good dispersity. Furthermore, its photocatalytic efficiency for degradation of Orange II and Methyl Orange under UV irradiation is 2.54 and 1.95 times higher than normal polythiophene composites relatively. According to the testment by Accelerated Surface Area and Porosimetry System, The true cause lay in the fact that the specific surface area of Polythiophene nanoparticles is 106.6 m2/g compared to normal Polythiophene is 24.2 m2/g, This conjugated polymer characterized by FTIR spectroscopies before and after photocatalytic reactions shows reliable chemical stability. In addition, it held excellent recovery ability and kept up their catalytic activity with indistinctive drop after six repeated utilization.

THE ADSORPTION OF METHYL ORANGE BY LYSOZYME

1954 ◽  
Vol 32 (1) ◽  
pp. 109-118
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Ionic Strength ◽  
Adsorption Isotherms ◽  
Inflection Point

Adsorption isotherms for methyl orange on lysozyme at ionic strengths varying from 0.001 to 0.05, pH 5.5, are sigmoid. Increasing ionic strength shifts the inflection point of such isotherms to higher free anion concentrations. Binding of one methyl orange anion to a lysozyme molecule in 0.05 M acetate, pH 5.5, facilitates adsorption of nine others, with subsequent precipitation of the protein. This co-operative behavior is interpreted on the basis of a previously described theory of interacting hydration effects. The possible biological implications for similar systems are indicated.

Chronoabsorptometric determination of adsorption isotherms for cytochrome c on tin oxide electrodes

Langmuir ◽  
1992 ◽  
Vol 8 (10) ◽  
pp. 2552-2559 ◽  
Author(s):  
Maryanne Collinson ◽  
Edmond F. Bowden
Keyword(s):  
Cytochrome C ◽  
Adsorption Isotherms ◽  
Tin Oxide ◽  
Oxide Electrodes
Sign in / Sign up

Export Citation Format

Share Document