THE ADSORPTION OF METHYL ORANGE BY LYSOZYME

1954 ◽  
Vol 32 (1) ◽  
pp. 109-118
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Ionic Strength ◽  
Adsorption Isotherms ◽  
Inflection Point

Adsorption isotherms for methyl orange on lysozyme at ionic strengths varying from 0.001 to 0.05, pH 5.5, are sigmoid. Increasing ionic strength shifts the inflection point of such isotherms to higher free anion concentrations. Binding of one methyl orange anion to a lysozyme molecule in 0.05 M acetate, pH 5.5, facilitates adsorption of nine others, with subsequent precipitation of the protein. This co-operative behavior is interpreted on the basis of a previously described theory of interacting hydration effects. The possible biological implications for similar systems are indicated.

THE ADSORPTION OF METHYL ORANGE BY LYSOZYME

1954 ◽  
Vol 32 (2) ◽  
pp. 109-118 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Ionic Strength ◽  
Adsorption Isotherms ◽  
Inflection Point

Adsorption isotherms for methyl orange on lysozyme at ionic strengths varying from 0.001 to 0.05, pH 5.5, are sigmoid. Increasing ionic strength shifts the inflection point of such isotherms to higher free anion concentrations. Binding of one methyl orange anion to a lysozyme molecule in 0.05 M acetate, pH 5.5, facilitates adsorption of nine others, with subsequent precipitation of the protein. This co-operative behavior is interpreted on the basis of a previously described theory of interacting hydration effects. The possible biological implications for similar systems are indicated.

scholarly journals The Passive Permeability of the Red Blood Cell to Cations

1966 ◽  
Vol 50 (1) ◽  
pp. 171-188 ◽  
Author(s):  
Paul L. LaCelle ◽  
Aser Rothstein
Keyword(s):  
Ionic Strength ◽  
Inflection Point ◽  
Ph Effect ◽  
Human Red Blood Cells ◽  
Low Concentrations ◽  
Goldman Equation ◽  
Low Ionic Strength ◽  
Straight Lines ◽  
The Relationship ◽  
Determining Factor

The efflux of salt from human red blood cells suspended in isotonic sucrose plus low concentrations of salt, was measured under steady-state conditions. The relationship between the efflux and the log of the salt concentration can be fitted by two straight lines with a sharp inflection point, the steeper slope occurring at concentrations below 0.2 mM NaCl. The determining factor in the rate of efflux is the ionic strength rather than the specific monovalent cations or anions and the effects are completely reversible. With an increase in temperature, the effects of reduced ionic strength are more pronounced and the inflection point is shifted toward higher salt concentrations. An increase in pH leads to an increased efflux at a given ionic strength, but the size of the pH effect is small at low ionic strength. At a given pH, the data can be fitted by a simplified form of the Goldman equation suggesting that with reduction in ionic strength, the permeability remains constant until the inflection point is reached. At that ionic strength, a sharp reversible transition to a new permeability state occurs. The permeability increases with an increase in the external but not the internal pH.

scholarly journals "Study the Effect of Immobilization on Electrostatic Potential for Methyl Orange Dye "

2018 ◽  
Vol 5 (1) ◽  
pp. 38-43
Author(s):  
Haider Shanshool Mohammed
Keyword(s):  
Sodium Chloride ◽  
Methyl Orange ◽  
Ionic Strength ◽  
Aqueous Solutions ◽  
Electrostatic Potential ◽  
Chloride Solution ◽  
Sodium Chloride Solution ◽  
Glass Wool ◽  
Modified Chitosan ◽  
Methyl Orange Dye

"In the current study, the effect of the immobilization on electrostatic potential, pH and the absorbance of aqueous solutions of methyl orange that was immobilized on a modified chitosan polymer by glass wool were studied. It was found that the values of these parameters of the supported methyl orange are increased compared to unsupported samples. Furthermore, the electrostatic potential of the supported methyl orange is investigated in the presence of an aqueous solutions of NaCl, where showed the value of the electrostatic potential is raised With increasing ionic strength of the sodium chloride solution and a decrease in the value of pKa to the methyl orange dye after immobilization while the results are inversely before immobilization methyl orange on a modified chitosan polymer by glass wool.

scholarly journals Effects of Ionic Strength, Temperature, and Urea on Behaviors of Methyl Orange-Dodecyltrimethylammonium Complex.

1993 ◽  
Vol 49 (4) ◽  
pp. 176-181 ◽  
Author(s):  
Masaru Mitsuishi ◽  
Daisuke Yoshida ◽  
Syouichi Urata ◽  
Kunihiro Hamada ◽  
Tsutomu Ishiwatari
Keyword(s):  
Methyl Orange ◽  
Ionic Strength

ADSORPTION OF ORANGE II BY CYTOCHROME c AND RIBONUCLEASE

1954 ◽  
Vol 32 (3) ◽  
pp. 184-188 ◽  
Author(s):  
J. Ross Colvin
Keyword(s):  
Methyl Orange ◽  
Cytochrome C ◽  
Adsorption Isotherms ◽  
Acetate Buffer ◽  
Orange Ii

The adsorption isotherms of Orange II on cytochrome c and ribonuclease at pH 5.5 in 0.05 M acetate buffer are sigmoid. They may be interpreted by a previously described theory of interacting hydration effects. Adsorption of methyl orange or sodium flavianate by either protein was negligible.

scholarly journals Adsorptive Removal of Antibiotic Ciprofloxacin from Aqueous Solution Using Protein-Modified Nanosilica

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 57 ◽  
Author(s):  
Tien Duc Pham ◽  
Thi Ngan Vu ◽  
Hai Long Nguyen ◽  
Pham Hai Phong Le ◽  
Thi Sim Hoang
Keyword(s):  
Ionic Strength ◽  
Adsorption Isotherms ◽  
High Performance ◽  
Maximum Adsorption Capacity ◽  
Adsorption Mechanisms ◽  
Adsorptive Removal ◽  
Step Model ◽  
Anionic Species ◽  
Pseudo Second Order ◽  
Ft Ir

The present study aims to investigate adsorptive removal of molecular ciprofloxacin using protein-modified nanosilica (ProMNS). Protein was successfully extracted from Moringa seeds while nanosilica was synthesized from rice husk. Fourier-transform infrared (FTIR), ultraviolet visible (UV-Vis) and high-performance liquid chromatography (HPLC) were used to evaluate the characterization of protein. Adsorption of protein onto nanosilica at different pH and ionic strength was thoroughly studied to modify nanosilica surface. The removal efficiency of antibiotic ciprofloxacin (CFX) increased from 56.84% to 89.86% after surface modification with protein. Effective conditions for CFX removal using ProMNS were systematically optimized and found to be pH 7.0, adsorption time 90 min, adsorbent dosage 10 mg/mL, and ionic strength 1 mM KCl. A two-step model was successfully used to fit the adsorption isotherms of CFX onto ProMNS at different ionic strength while a pseudo-second-order model could fit adsorption kinetic of CFX onto ProMNS very well. Maximum adsorption capacity was very high that reached to 85 mg/g. Adsorption of CFX onto ProMNS decreased with increasing KCl concentration, suggesting that adsorption of CFX onto ProMNS is mainly controlled by electrostatic attraction between positively charged ProMNS surface and anionic species of CFX. Adsorption mechanisms of CFX onto ProMNS were discussed in detail based on adsorption isotherms, the change in surface charge by zeta potentail and the change in functional groups by FT-IR. The removal of CFX after three regenerations was greater than 73% while CFX removal from an actual hospital wastewater using ProMNS reached to 70%. Our results suggest that ProMNS is a new and eco-friendly adsorbent to remove antibiotics from aqueous solutions.

Comparison of Technics for Estimation of Albumin by Determination of Its Dye-Binding Capacity and by Paper Electrophoresis

1964 ◽  
Vol 10 (4) ◽  
pp. 309-320 ◽  
Author(s):  
Jesse F Goodwin
Keyword(s):  
Methyl Orange ◽  
Ionic Strength ◽  
Benzoic Acid ◽  
Binding Capacity ◽  
Paper Electrophoresis ◽  
Free Hemoglobin ◽  
System A ◽  
Dye Absorption ◽  
Electrophoresis System

Abstract Values for serum albumin obtained by the methyl orange and 2-(4’-hydroxyazobenzene) benzoic acid (HABA) dye-absorption methods have been compared with values obtained by Spinco Procedure B paper-electrophoresis system. A good correlation of albumin values has been found by the three methods in serums with albumin/globulin ratios ranging from 0.19 to 3.00. Modifications of the dye-absorption procedures are presented which permit use of reagents ordinarily employed for automatic analyses on small numbers of specimens. A decrease in ionic strength of the methyl orange buffer tends to increase the sensitivity of this procedure. Temperature affects the HABA dye/albumin reaction. Bilirubin, free hemoglobin, and lipid interfere with both dye procedures. Means for eliminating most of these interferences have been presented. The methyl orange procedure appears to be superior to the 2-(4 -hydroxyazobenzene) benzoic acid method for estimating albumin in icteric serum and serum containing macroglobulins. Albumin values of serum containing abnormal proteins were compared with values obtained by paper electrophoresis and both dye procedures. Estimation of albumin by the two dye procedures appears to be simple, precise, and fairly specific.

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