Multivitamin and mineral supplementation in 1,2-dimethylhydrazine induced experimental colon carcinogenesis and evaluation of free radical status, antioxidant potential, and incidence of ACF

2012 ◽  
Vol 90 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Albert Baskar Arul ◽  
Ignacimuthu Savarimuthu ◽  
Mohammed A. Alsaif ◽  
Khalid S. Al Numair
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Colon Carcinogenesis ◽  
Aberrant Crypt Foci ◽  
Lipid Hydroperoxides ◽  
Entire Study ◽  
Lipid Peroxidation Products ◽  
Histopathological Alterations ◽  
Mineral Supplementation

This study was performed to determine the chemopreventive and antioxidant status of multivitamin and mineral (0.01% in drinking water, ad libitum) supplements in 1,2-dimethylhydrazine (DMH)-induced experimental colon carcinogenesis. Experimental colon carcinogenesis was induced in male albino Wistar rats by injecting DMH (20 mg·(kg body mass)–1) once weekly for 15 consecutive weeks, and administering a multivitamin supplement in 3 regimes (initiation, post-initiation, and entire experimental period) for 32 weeks. We studied lipid peroxidation products (thiobarbituric acid reactive substances, lipid hydroperoxides, conjugated dienes) in the circulation and in the tissues, antioxidant status (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and non-enzymatic antioxidant-reduced glutathione) of the tissues, aberrant crypt foci (ACF), and histopathological alterations. DMH-induced rats had an increase in lipid peroxidation products and a lower antioxidant status compared with control animals. Multivitamin and mineral supplementation during the initiation, post-initiation, and the entire study period significantly decreased the levels of lipid peroxidation products in circulation and colonic tissues, significantly elevated the activities of the antioxidant enzymes and reduced glutathione to near normalcy in DMH-induced rats. The incidence of ACF was reduced to 84.1% in rats supplemented with multivitamin and minerals for the entire study and prevented the colonic tissue from histopathological alterations induced by DMH.

Chemopreventive efficacy of pronyl-lysine on lipid peroxidation and antioxidant status in rat colon carcinogenesis

2009 ◽  
Vol 23 (3) ◽  
pp. 293-302 ◽  
Author(s):  
Jayabal Panneer Selvam ◽  
Selvaraj Aranganathan ◽  
Rama Gopalan ◽  
Namasivayam Nalini
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Colon Carcinogenesis ◽  
Rat Colon

scholarly journals Influence of Marmix premix on the state of lipid peroxidation and indices of non-specific resistance of the organism of pregnant mares with microelementosis

2019 ◽  
Vol 10 (1) ◽  
pp. 87-91
Author(s):  
A. R. Shcherbatyy ◽  
L. G. Slivinska ◽  
B. V. Gutyj ◽  
V. L. Fedorovych ◽  
B. O. Lukashchuk
Keyword(s):  
Lipid Peroxidation ◽  
Blood Serum ◽  
Immune Complexes ◽  
Positive Influence ◽  
Lysozyme Activity ◽  
The State ◽  
Circulating Immune Complexes ◽  
Lipid Hydroperoxides ◽  
Diene Conjugates ◽  
Lipid Peroxidation Products

We observed the processes of lipid peroxidation, characterized by increased content of intermediate and its end products (diene conjugates, lipid hydroperoxides, malonic dialdehyde), in the blood of the pregnant mares. The changes of the data of indicators in the organism of the mares at the 9–11 months of pregnancy were established. The level of diene conjugates in the blood of animals was 3.6 ± 0.12 μmol/l, 33.3 and 44.0%, which was higher compared to non-pregnant mares and mares at 4th month of pregnancy. The concentration of lipid hydroperoxides in the blood of mares increased by 100% and by 42.9% in the blood of non-pregnant mares and mares at 4th month of pregnancy. The concentration of malondialdehyde in the blood of mares increased by 75.0%, 51.2% and 25.0% compared to non-pregnant mares and mares at 4th and 7th month of pregnancy, respectively. The results of our research showed that the mineral-vitamin premix Marmix had a positive influence on the state of lipid peroxidation products, contributed to their lowering in the blood of pregnant mares, which demonstrated antioxidant action and stimulated the function of the immune system. It was established that the bactericidal and lysozyme activity of the blood serum and the concentration of circulating immune complexes increases in the mares with lack of trace elements. It was shown that feeding mares during the 9–11 months of pregnancy with the mineral-vitamin premix Marmix had a positive influence on the state of humoral factors of nonspecific resistance. In particular, the concentration of bactericidal activity of serum increased by 31.0%, lysozyme activity of blood serum – 45.4% and contributed to the decrease of the concentration of circulating immune complexes in serum by 3.8 times. The use of mineral-vitamin premix Marmix during 60 days in feeding pregnant mares caused a restoration of the clinical status, a decrease in the content of lipid peroxidation products.

Increased erythrocyte antioxidant status protects against smoking induced hemolysis in moderate smokers

2011 ◽  
Vol 30 (10) ◽  
pp. 1475-1481 ◽  
Author(s):  
Padmavathi Pannuru ◽  
Damodara Reddy Vaddi ◽  
Rameswara Reddy Kindinti ◽  
Nallanchakravarthula Varadacharyulu
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Antioxidant Status ◽  
Reduced Glutathione ◽  
Membrane Lipid ◽  
Red Cell ◽  
Membrane Lipid Peroxidation ◽  
Free Radical Damage ◽  
Human Red Blood Cells ◽  
Nitrite Nitrate

Cigarette smoking is common in societies worldwide and has been identified as injurious to human health. Human red blood cells are important targets for electrophilic and oxidant foreign compounds. In the present study, the possible role of antioxidant status on smoking-induced erythrocyte hemolysis of smokers was studied. Erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH) level, erythrocyte membrane lipid peroxidation, total cholesterol and phospholipids were determined. Further, nitrite/nitrate levels (NO2/NO3) in both plasma and erythrocyte lysate were measured. Results showed increased plasma and erythrocyte membrane lipid peroxidation and nitrite/nitrate levels in smokers. The activities of SOD, CAT and GPx were also increased with reduced glutathione (GSH) level in smokers. No significant change was observed in smokers red cell hemolysis and cholesterol/phospholipid (C/P) ratio compared to controls. Erythrocyte membrane lipid peroxidation was positively correlated with SOD ( r = 0.482, p < 0.01) and GPx ( r = 0.368, p < 0.018) in smokers. Increased levels of nitrite/nitrate and antioxidant status of erythrocytes might be playing a crucial role in protecting red cell from free radical damage induced by cigarette smoke.

scholarly journals Effects of peroxidation products in thermoxidised dietary oil in female rats during rearing, pregnancy and lactation on their reproductive performance and the antioxidative status of their offspring

2004 ◽  
Vol 92 (2) ◽  
pp. 267-275 ◽  
Author(s):  
Corinna Brandsch ◽  
Klaus Eder
Keyword(s):  
Lipid Peroxidation ◽  
Reproductive Performance ◽  
Antioxidant Status ◽  
Energy Content ◽  
Female Rats ◽  
High Concentration ◽  
Antioxidative Status ◽  
Lipid Peroxidation Products ◽  
Pregnancy And Lactation ◽  
Dietary Oil

The present study was performed to investigate whether lipid peroxidation products in thermoxidised dietary oil fed during rearing, pregnancy and lactation influences the reproductive performance of female rats and the antioxidant status of their offspring. Twenty-four female rats were divided into two groups at 4 weeks of age. They were fed diets containing fresh or oxidised oil (the latter prepared by heating at a temperature of 50°C for 16 d) for 14 weeks. At the age of 12 weeks female rats were mated. The number of total pups and pups born alive was not different between both groups. However, individual pups and litters of dams fed oxidised oil were lighter at birth and gained less weight during the suckling period than those of dams fed fresh oil (P>0·05). Pups of dams fed oxidised oil contained less protein and more fat in their carcasses than those of dams fed fresh oil (P>0·05). The milk of dams fed oxidised oil had a lower concentration of triacylglycerols and a lower energy content than that of dams fed the fresh oil (P>0·05). The pups of dams fed oxidised oil had higher concentrations of lipid peroxidation products in the liver at birth and day 19 of lactation than those of dams fed fresh oil (P>0·05). In conclusion, the present study shows that feeding oxidised oil with a high concentration of lipid peroxidation products to female rats during rearing, pregnancy and lactation influences the development and antioxidant status of fetus and suckling pups.

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