The effects of aluminum on Picearubens:factorial experiments using sand culture
We used sand culture to examine the effects of aluminum (Al3+) on gas exchange and growth in 1+0 Picearubens Sarg. seedlings. Growth solutions were prepared to match the elemental concentrations of the soil solutions in a declining Abiesbalsamea (L.) Mill.–P. rubens stand. In our first experiment, we used a four-way factorial design to examine the interactions between Al, pH, nutrients, and CO2. All plants were nitrogen deficient. Photosynthetic rate and instantaneous water-use efficiency both declined at 250 μmol•L−1 Al, as did the root/shoot ratio of the seedlings. Plant weight, however, was not significantly affected by the 10-fold increase in Al solution concentration. These results were robust across a range of experimental conditions, suggesting that other aspects of soil acidification, such as NO3 fertilization in N deficient soils, could counteract the toxic effects of Al. In a second experiment, we examined the effects of subtoxic concentrations of Al at two levels of nutrient cation availability. Unlike in a previous study, cation fertilization affected neither gas exchange nor growth. While the decreases in root/shoot ratio and growth were expected at 250 μmol•L−1 Al, the peak in growth rates for intermediate concentrations of Al (25–100 μmol•L−1) was not. The latter result was interpreted in light of the positive effects that low concentrations of Al are known to have on other species.