Growth and ectomycorrhiza formation of container-grown red oak seedlings as a function of nitrogen fertilization and inoculum type of Laccariabicolor

1991 ◽  
Vol 21 (7) ◽  
pp. 966-973 ◽  
Author(s):  
J. Gagnon ◽  
C. G. Langlois ◽  
J. Garbaye
Keyword(s):  
Growth Parameters ◽  
Peat Moss ◽  
Red Oak ◽  
Shoot Height ◽  
Root Collar Diameter ◽  
N Level ◽  
Collar Diameter ◽  
Mycelial Suspension ◽  
Root Collar ◽  
Oak Seedlings

Containerized red oak (Quercusrubra L.) seedlings were inoculated at the time of sowing with Laccariabicolor (Maire) Orton using two methods to compare the efficiency of two inoculum types: a mycelial suspension (Ecot-Sol®101) produced by Rhizotec Laboratories Inc. and calcium alginate beads containing this mycelial suspension. Red oak seedlings were also grown for 19 weeks in a peat moss – vermiculite substrate under three levels of N fertilization (100, 120, and 140 mg/seedling per season) to determine the N level that maximizes the ectomycorrhizae formation and growth of seedlings. After 19 weeks in the greenhouse, seedlings inoculated with liquid inoculum had significantly more mycorrhizae than both those inoculated with beads and controls, regardless of the N level. For any of the N levels, liquid-inoculated seedlings had significantly lower shoot height, root-collar diameter, and dry weights (shoot, root, and total) than both those inoculated with beads and the controls, whereas there were no significant differences for any growth parameters between the bead and control treatments. For the three inoculum treatments, root and total dry weights of seedlings fertilized with 100 mg N were significantly lower than those of seedlings that received both 120 and 140 mg N, whereas shoot height, root-collar diameter, and shoot:root ratio of seedlings did not differ significantly between any of the three N levels. After 19 weeks, seedlings inoculated with liquid inoculum had significantly greater N and P concentrations (%) and contents (mg/seedling) than those inoculated with beads only at the 140 mg N level. Analyses show that seedling concentrations of 1.2% N and 0.1% P and substrate fertility of 25 ppm N and 30 ppm P would be appropriate to maintain the ectomycorrhizal association Q. rubra – L. bicolor.

Growth and ectomycorrhizal formation of container-grown Douglas-fir seedlings inoculated with Laccariabicolor under four levels of nitrogen fertilization

1995 ◽  
Vol 25 (12) ◽  
pp. 1953-1961 ◽  
Author(s):  
J. Gagnon ◽  
C.G. Langlois ◽  
D. Bouchard ◽  
F. Le Tacon
Keyword(s):  
Growth Parameters ◽  
N Fertilization ◽  
Douglas Fir ◽  
Peat Moss ◽  
Mycorrhizal Infection ◽  
Shoot Height ◽  
N Level ◽  
Four Levels ◽  
Substrate Fertility ◽  
Mycelial Suspension

Container-grown Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings were inoculated at the time of sowing with a Laccariabicolor (Maire) Orton mycelial suspension produced in a fermentor. They were grown in a peat moss–vermiculite substrate under four levels of N fertilization (7.2, 14.4, 21.6, and 28.7 mg/seedling per season (N1, N2, N3, and N4, respectively)) to determine the N level suitable for both ectomycorrhizal development and seedling growth. After 18 weeks in the greenhouse, seedlings inoculated with L. bicolor had 44%, 32%, 44%, and 5% of their short roots mycorrhizal when fertilized with N1, N2, N3, and N4, respectively. Only when they were fertilized with N4 did the L. bicolor seedlings have significantly greater shoot height than the controls. For the other growth parameters, they were not significantly different from control seedlings for any of the N levels. After 18 weeks, regardless of the level of N, seedlings inoculated with L. bicolor had significantly lower N concentrations (%) and contents (mg/seedling) than the uninoculated ones. Consequently, for the same production of biomass, the mycorrhizal seedlings had taken up less N than the nonmycorrhizal ones. The efficiency of applied N, expressed in terms of produced biomass, decreased when the N fertilization increased; mycorrhizal and nonmycorrhizal seedlings did not tend to be different. The efficiency of the absorbed N also decreased with the level of applied N, but less rapidly, and tended to be greater for the mycorrhizal seedlings than for the nonmycorrhizal ones. Therefore, the mycorrhizal infection improved the utilization of the absorbed N. N3 was the best of the four N levels used, since it was the only one that maximized both the ectomycorrhizal formation and the growth of the seedlings. In other words, a total seedling N concentration of 1.6% and a substrate fertility of 52 ppm N are appropriate to optimize both the ectomycorrhizal development and the growth of Douglas-fir seedlings.

scholarly journals Variability of some morphological traits of one-year old red oak, black walnut, birch and wild pear seedlings in the nurseries of Jastrebac region

2002 ◽  
pp. 147-160
Author(s):  
Milun Krstic ◽  
Nevena Vojinovic
Keyword(s):  
Comparative Analysis ◽  
Root Length ◽  
Black Walnut ◽  
Red Oak ◽  
Root Collar Diameter ◽  
Significant Difference ◽  
Collar Diameter ◽  
One Year ◽  
Root Collar ◽  
Oak Seedlings

Five morphological and quantitative characteristics of one-year old seedlings of Red oak (Quercus rubra L), Black walnut (Juglans nigra L), Wild pear (Pyrus pygrowser Borkh) and Birch (Betula verrucosa Ehrh) were studied. The seedlings were produced and cultivated in the controlled conditions of the nursery in the region of Jastrebac, by the classical method. Aboveground seedling height, root collar diameter, root length, number of secondary roots and the leaf assimilation area were analysed. Intraspecific and interspecific variability of morphological features of the above species were assessed by the comparative analysis and statistical methods The comparative analysis shows the great individual variability of seedlings, which can indicate their genetic potential, adaptation to environment conditions, further spontaneous selection and the development in natural conditions. This justifies the need of the quality assessment and the first selection already in the nursery, in order to ensure the quality planting material and to reduce the risk of afforestation failure One-year old birch seedlings have the lowest average height (18.8 cm). Black walnut and Wild pear seedlings are approximately twice as high, and Red oak about 2.5 times higher. At the same time Red oak seedlings have for about one-fourth greater height than Black walnut, and for one-third greater height than Wild pear. Wild pear seedlings attain the averagely twice larger root collar diameter than Birch (2.8 cm), Red oak seedlings about 2.5 times larger diameter, and Black walnut 3.5 times larger diameter. Black walnut has a larger root collar diameter than Red oak for about one third, and almost twice larger than Wild pear. Birch, Red oak and Wild pear have almost twice longer root (1.8-1.9 times), Black walnut about 2.25 times longer. The total assimilation area of a Birch seedling is averagely 89.0 cm2. Compared to birch, wild pear has approximately double assimilation area per tree, Red oak 10 times larger, Black walnut even 14 times larger assimilation area The analysis of variance shows that the experiment is homogeneous in general, i.e. that variance within the group of the same species per all characteristics is not statistically significant at the level p>0.01. The statistically significant difference was found in the attained heights of one-year old seedlings of Red oak and Birch, as well as between them and other species, while there is no difference between Black walnut and Wild pear. All analysed species differ significantly in root collar diameter of one-year old seedlings. Black walnut and Birch differ significantly in root length, and also compared to other species. There are no statistically significant differences in root length between Red oak and Wild pear. All the analysed species differ statistically significantly per total assimilation area of one seedling .

L'utilisation du compost de boues de stations d'épuration des eaux comme substrat de culture pour la production des plants forestiers en récipients

1993 ◽  
Vol 20 (3) ◽  
pp. 518-527
Author(s):  
Patrick Polan ◽  
Jean Gagnon ◽  
J. Peter Jones
Keyword(s):  
Pinus Banksiana ◽  
Wastewater Treatment Plants ◽  
Growth Parameters ◽  
Jack Pine ◽  
Peat Moss ◽  
Shoot Height ◽  
Containerized Seedlings ◽  
Pine Seedlings ◽  
Sludge Recycling ◽  
Collar Diameter

The Quebec government has established a program to treat industrial and domestic wastewaters. However, this program does make it necessary to dispose of large quantities of sludge. This sludge can be burned or landfilled but such solutions do not properly draw benefit from the sludge's potential as a fertilizer. There is a considerable interest in composting of the sludge. In this study, compost from the composting plant in Plattsburgh was mixed with the growing substrate normally used for the production of containerized jack pine (Pinus banksiana Lamb.) seedlings to determine if the compost could be used for the production in containers of forest seedlings. The growth of containerized seedlings produced with proportions by volume of 80% peat moss and 20% vermiculite was compared to the growth of seedlings when the peat moss (80%) was replaced by different proportions of compost (20, 40, 60, and 80%). After one growing season in tunnel, the best growth rates in terms of shoot height, root-collar diameter, and dry biomass were obtained with the control, followed in decreasing order by the treatments compost 20, 40, 60, and 80%. In this experiment, the use of compost from wastewater treatment plants reduced significantly the growth of jack pine and this can be explained mainly by the lack of nitrogen in the tissues of jack pine seedlings. However, the adjustment of nitrogen fertilization and conditions of cultivation by using compost may permit the production of containerized seedlings having the same dimensions as the controls. The seedling growth parameters when 20% compost was incorporated in the substrate were nearly the same as the control seedling. Key words: sludge, recycling, compost, substrate, container, forest seedlings, jack pine.

scholarly journals Seedling Growth of Tectona grandis using Different Potting Mixture

2021 ◽  
pp. 17-24
Author(s):  
G. E. Omokhua ◽  
C. Fredrick ◽  
C. N. Okakpu
Keyword(s):  
Seedling Growth ◽  
Growth Parameters ◽  
Tectona Grandis ◽  
Dry Weight ◽  
Leaf Number ◽  
Cow Dung ◽  
Root Collar Diameter ◽  
Shoot Dry Weight ◽  
Collar Diameter ◽  
Root Collar

Aims: This study evaluated the influence of 11 potting media compositions on seedling growth and development of Tectona grandis. Study Design: The experiment was set up in a completely randomized design involving ten replicates. Place and Duration of Study: The study was carried out at the Department of Forestry and Wildlife Management Nursery, University of Port Harcourt, Rivers State, Nigeria, between April 2019 and April 2020. Methodology: Ten (10) seedlings per sowing media including topsoil, topsoil and cow dung (2:1, 3:1), topsoil and poultry dung (2:1, 3:1), topsoil and pig dung (2.1, 3:1), topsoil and sawdust (2.1, 3:1), topsoil and mushroom substrate (2.1, 3:1) were selected and transplanted into polybags. Growth parameters (seedling height, root collar diameter, leaf number and biomass (shoot dry weight (SDW), root dry weight (RDW) and total dry weight (TDW)) were determined and subjected to analysis of variance. Results: Seedlings of T. grandis displayed significant differences (p ≤ 0.05) in height, root collar diameter, leaf number and biomass. Among treatments, tallest seedlings were observed in mixture of topsoil and poultry dung (3:1), at months 1 to 3 and in mixture of topsoil and cow dung (3:1) at months 4 and 5, highest root collar diameter was observed in mixture of topsoil and poultry dung (3:1) and highest leaf number was recorded in mixture of topsoil and poultry dung (2:1 and 3:1) and topsoil and cow dung (2:1 and 3:1) at months 1 to 5. The highest SDW was evident in mixture of topsoil and poultry dung (2:1) while highest RDW and TDW were observed in mixture of topsoil and cow dung (3:1). Lowest height, diameter, leaf number and biomass were recorded in topsoil (control) at months 1 to 5. Conclusion: The results revealed that among the tested mixtures, topsoil and poultry dung and topsoil and cow dung mixtures were the best for seedlings growth of T. grandis.

scholarly journals Influence of acorn size on morphological characteristics of one-year-old Northern red oak (Quercus rubra L.) seedlings

2015 ◽  
Vol 67 (4) ◽  
pp. 1357-1360
Author(s):  
Vladan Popovic ◽  
Aleksandar Lucic ◽  
Ljubinko Rakonjac ◽  
Tatjana Cirkovic-Mitrovic ◽  
Ljiljana Brasanac-Bosanac
Keyword(s):  
Quercus Rubra ◽  
Morphological Characteristics ◽  
Red Oak ◽  
Northern Red Oak ◽  
Collar Diameter ◽  
Acorn Size ◽  
One Year ◽  
Root Collar ◽  
Oak Seedlings

The aim of this research was to examine the influence of acorn size on morphological characteristics of one-year-old Northern red oak (Quercus rubra L.) seedlings. The quality of seedlings correlates with the quality of the seed they are derived from. In species with large seeds, as in the case of Northern red oak, the seedling growth in the first growing season is closely related to seed size or the amount of reserve nutrients that are stored in the seed. The height and root collar diameter of one-year-old Northern red oak seedlings increases with increase in acorn size. The results obtained in this research can be used as a guideline for acorn grading, because they show that improved Northern red oak seedlings quality can be achieved using seeds of appropriate size.

scholarly journals Nursery Production of Pinus engelmannii Carr. with Substrates Based on Fresh Sawdust

Forests ◽  
2018 ◽  
Vol 9 (11) ◽  
pp. 678 ◽  
Author(s):  
María González-Orozco ◽  
José Prieto-Ruíz ◽  
Arnulfo Aldrete ◽  
José Hernández-Díaz ◽  
Jorge Chávez-Simental ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Production Costs ◽  
Pine Bark ◽  
Peat Moss ◽  
Root Collar Diameter ◽  
Pine Sawdust ◽  
Nursery Production ◽  
Biomass N ◽  
Collar Diameter ◽  
Root Collar

Substrate is a factor that significantly influences the quality and production costs of nursery seedlings. The objective of this study was to evaluate combinations of peat moss, composted pine bark, and fresh pine sawdust in order to identify the proportions that favour the quality of Pinus engelmannii Carr. seedlings and minimise the production costs in the nursery. Substrates were formed using mixtures of peat moss (15% to 50%), composted pine bark (15% to 50%) and fresh pine sawdust (20% to 70%), with 2, 4 and 6 g L−1 of controlled release fertilizer (Multicote®, Haifa, Israel). A completely randomised experimental design with a factorial arrangement of 7 × 3 was used. The evaluated factors are root collar diameter, biomass, N-P-K content, and production costs of the substrates which were determined based on the container volume and three commercial quotations. Significant differences were found in root collar diameter and biomass, highlighting the treatments using 50% to 70% sawdust with 6 g L−1 of fertilizer. Assimilated values of N-P-K were acceptable in all treatments with 4 and 6 g L−1 of fertilizer. In the substrates with high percentages of sawdust, seedlings with morphological characteristics and nutritional levels within the values recommended for conifers were produced. In addition, it was possible to reduce the production cost of the substrates by up to 67%.

scholarly journals An Assessment of the Benefits and Period of Arbuscular Mycorrhizal Fungi in Plant Growth Regulation and the Level of Fertilizer Influential Tomato Development (Solanum lycopersicum L.)

2020 ◽  
pp. 20-33
Author(s):  
Ouattara Brahima ◽  
Abo Kouabenan ◽  
Tuo Seydou ◽  
Silue Nakpalo ◽  
Kone N’golo Abdoulaye ◽  
...  
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Growth Regulation ◽  
Growth Parameters ◽  
Arbuscular Mycorrhizal ◽  
Root Collar Diameter ◽  
Collar Diameter ◽  
The Impact ◽  
Root Collar

Data on tomato fitness improvement by arbuscular mycorrhizal fungi (AMF) remain patchy. The present study was initiated to evaluate the effect of the period of AMF inoculation as well as the level of mineral manure on tomato growth. The experiment took place from June to October 2016, in the West African Science Service Center on Climate Change and Adapted Land Use greenhouse. AMF inocula were applied to seeds and/or transplants, each receiving three different levels of chemical fertilizer. The impact of the inoculation period and the level of fertilization, were assessed on plant growth parameters, including height, number of functional leaves, root-collar diameter, and root length. Observation of hyphae, arbuscules and vesicles was carried out by roots staining method and anabled the determination of mycorrhization parameters. Plants Mycorrhizal dependence was assessed with their fresh and dry mass. An analysis of variance and post ANOVA analysis was performed using the Newman-Keuls test (P= .05) for the comparison of means. The findings pointed that, when transplanting, the difference between mycorrhized plants and non-mycorrhized ones was very highly significant in terms of the height of the stem (P= .00), the length of the taproot, and the root collar diameter. The lower the level of manure was, the higher the frequency of infection has been (73.33% for MS1 and MSR1; 76.67% for MR1).Transplants growing without a supply of mineral manure expressed greater mycorrhizal dependence (66% for MSR1). Arbuscular mycorrhization of the tomato is profitable for its optimal development. The endomycorrhization of tomato can be done during sowing or transplanting with the same benefits but, with a low level of fertilizer. So, it’s necessary to controll the intake of mineral manure because it influences the natural mycorrhization of plants.

Responses of northern red oak seedlings to lime and deer exclosure fencing in Pennsylvania

2012 ◽  
Vol 42 (4) ◽  
pp. 698-709 ◽  
Author(s):  
Robert P. Long ◽  
Patrick H. Brose ◽  
Stephen B. Horsley
Keyword(s):  
Red Oak ◽  
Northern Red Oak ◽  
Growth Responses ◽  
Deer Browsing ◽  
Root Collar Diameter ◽  
Growth And Nutrition ◽  
Forest Liming ◽  
Collar Diameter ◽  
Wire Fence ◽  
Root Collar

In Pennsylvania, two hypotheses compete to explain the chronic oak ( Quercus spp.) regeneration problem: excessive deer browsing and soil cation depletion. We tested these hypotheses by evaluating the effect of forest liming and deer exclosure fencing on northern red oak ( Quercus rubra L.) seedling growth and nutrition in five oak shelterwood stands in Pennsylvania over 6 years. In each stand, four planting plots were located inside a 2.4 m high woven wire fence and another four were established outside the fence. About 225 northern red oak acorns were planted in each plot in spring 2004. Dolomitic limestone was applied to randomly selected plots at rates of 0, 4.5, 9.0, and 13.5 Mg·ha–1 during May 2004. There were no statistically significant (P ≤ 0.05) growth responses to lime applications. The only significant growth responses resulted from the fence versus no-fence treatment. A significant (P < 0.003) fence × year interaction for seedling height and root collar diameter indicates differential impacts of deer browsing. By 2009, seedlings inside fences averaged 32 cm tall, while seedlings outside the fences averaged 17 cm. Similarly, root collar diameter averaged 6.6 mm outside the fences and 9.1 mm inside fences.

scholarly journals Growth of bare root Pinus taeda, L. seedlings cultivated under five densities in nursery

2007 ◽  
Vol 64 (1) ◽  
pp. 23-29
Author(s):  
José Geraldo de Araújo Carneiro ◽  
Deborah Guerra Barroso ◽  
Luis Maurício da Silva Soares
Keyword(s):  
Pinus Taeda ◽  
Dry Matter ◽  
Root Systems ◽  
Root Collar Diameter ◽  
Plant Densities ◽  
Collar Diameter ◽  
Bare Root ◽  
Pinus Taeda L ◽  
Root Collar ◽  
Dry Matter Weight

Seedlings compete for nutrients, water and light. The available area for each seedling affects their behavior related to requirements for these resources. This experiment evaluated the influence of five plant densities on the growth of bare root Pinus taeda, L. seedlings in a nursery after outplanting. The analyzed characteristics were: height (H), root collar diameter (D), H/D ratio, and dry matter weight. Higher densities stimulated H growth and the lowest densities increased D average and dry matter weight and lowered the H/D ratio. Seedlings were distributed by H, D and H/D classes. Higher densities had a larger number of seedlings in larger H classes. Larger numbers of seedlings with larger D and lower H/D ratios were found in lower densities. Ten months after outplanting the seedlings grown in lower densities had higher survival percentages and growth. Some saplings of standardized heights were uprooted with the objective of studying their root systems. The lowest densities stimulated higher numbers of first and second order roots as well as fresh and dry matter weights of thin roots with mycorrhizae presence. In both parts of the experiment, the density of 278 seedlings m-2 yielded equivalent averages as compared to the lowest densities.

scholarly journals Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

2019 ◽  
Vol 11 (10) ◽  
pp. 277 ◽  
Author(s):  
Meire Pereira de Souza Ferrari ◽  
Mayara dos Santos Queiroz ◽  
Matheus Marquezini de Andrade ◽  
Jessica Rezende Trettel ◽  
Hélida Mara Magalhães
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Activity ◽  
Growth Regulators ◽  
Curcuma Longa ◽  
Ms Medium ◽  
Biochemical Activity ◽  
Root Collar Diameter ◽  
Collar Diameter ◽  
Root Collar

This study aimed to evaluate the in vitro growth and biochemical activity of Curcuma longa explants using different MS medium formulations and growth regulators. In all the experiments, plants were grown in MS medium supplemented with agar (6.5 g L-1) and pH adjusted to 5.8. In the first assay, the MS culture medium at 70% strength, supplemented with 30 g L-1 of sucrose, and without the addition of activated carbon, resulted in the highest number of shoots. The sucrose concentration of 60 g L-1, combined with the addition of actived charcoalin half-strength MS medium, resulted in the increased root dry mass, root collar diameter, and relative chlorophyll index. In the second assay, the highest root collar diameter and dry matter of shoots and roots were found in the MS medium supplemented with 4.44 BAP, 0.46 KIN, and 1.08 NAA. The MS medium with 8.88 BAP, 0.92 KIN, and 2.16 &mu;M NAA resulted in the highest number of shoots (7.75), number of leaves (35), and shoot length (88.57 mm). The antioxidant activity was significantly higher in the treatments that resulted in better plantlets growth performance, demonstrating that the antioxidant activity is related to other factors such as a possible role of growth regulators on the elicitation of compounds in plants. Superoxide dismutase had a high enzymatic activity in both assays, whereas the enzymatic activity of catalase and ascorbate peroxidase was dependent on the culture media used.

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