Invitro plant regeneration via callus culture of mature Salixexigua

1989 ◽  
Vol 19 (12) ◽  
pp. 1634-1638 ◽  
Author(s):  
Michael U. Stoehr ◽  
Mantang Cai ◽  
Louis Zsuffa
Keyword(s):  
Woody Plant ◽  
Leaf Explants ◽  
Plantlet Regeneration ◽  
Clonal Variation ◽  
Dichlorophenoxyacetic Acid ◽  
Shoot Induction ◽  
Callus Production ◽  
Woody Plant Medium ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid

Callus induction, callus growth, and plantlet regeneration were examined using leaf explants of three Salixexigua clones. Calli were initiated on three basal media supplemented with 0.1 mg/L (0.44 μM) or 0.5 mg/L (2.2 μM) of benzylaminopurine and 0.1 mg/L (0.45 μM) or 0.5 mg/L (2.3 μM) of 2,4-dichlorophenoxyacetic acid in a factorial fashion. After 7 weeks of growth, callus production was highest on woody plant medium. Developed calli were subsequently cultured on woody plant medium supplemented with either 0.1 or 0.5 mg/L of benzylaminopurine for shoot induction. Shoot primordia developed only at 0.1 mg/L (0.44 μM) benzylaminopurine in two clones, suggesting clonal variation in organogenic response. Shoots larger than 1.0 cm in length were successfully rooted in half-strength Murashige and Skoog medium without hormones.

Plantlet production from anthers of Eastern cottonwood (Populusdeltoïdes)

1988 ◽  
Vol 18 (7) ◽  
pp. 937-941 ◽  
Author(s):  
M. Rafique Uddin ◽  
Martin M. Meyer Jr. ◽  
J. J. Jokela
Keyword(s):  
Butyric Acid ◽  
Woody Plant ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Eastern Cottonwood ◽  
Skoog Medium ◽  
Plantlet Production ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
2,4 Dichlorophenoxyacetic Acid

Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excising and transferring to woody plant medium supplemented with 1.0 μM indole-3-butyric acid. A few of these plants were found to be haploid. Two plants developed male terminal inflorescences, but died shortly thereafter.

In vitro culture of arbuscular mycorrhizal fungus and Frankia for inoculation of micropropagated Casuarina equisetifolia L.

1999 ◽  
Vol 77 (9) ◽  
pp. 1391-1397
Author(s):  
Genevieve Louise Mark ◽  
John E Hooker ◽  
Alexander Hahn ◽  
Chris T Wheeler
Keyword(s):  
Spore Germination ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Dichlorophenoxyacetic Acid ◽  
Casuarina Equisetifolia ◽  
Half Strength ◽  
2,4 Dichlorophenoxyacetic Acid

Micropropagated, rooted, and calli explants of Casuarina equisetifolia L. were inoculated with Frankia UGL 020605S and the arbuscular mycorrhizal fungus (AMF) Glomus mosseae, in single and dual co-culture, in vitro. Different micropropagation media formulations were evaluated for their capacity to stimulate germination of G. mosseae spores and growth of Frankia. Murashige and Skoog basal nutrient (half strength) medium, supplemented with 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), and pyruvate was selected for the in vitro co-culture of C. equisetifolia callus explants, G. mosseae, and Frankia. This medium (M4) supported 70% AMF spore germination with 44 and 34% of the germinating spores producing single and branched hyphal strands, respectively. Hoaglands (quarter strength, modified by Hoaglands and Arnon (1950)) nutrient medium (M5) with no supplements was selected for the in vitro co-culture of rooted C. equisetifolia explants, G. mosseae, and Frankia and supported 57% AMF spore germination with 29 and 40% of the germinating spores producing single and branched hyphal strands, respectively. Both media supported significant growth of Frankia. In both cases agar was substituted with Terragreen(r). AMF appressoria and intercellular hyphae were observed in rooted C. equisetifolia at 28 days; arbuscule formation occurred at 56 days postinoculation. Frankia infection was evident after 28 days. This was observed in both dual and single in vitro co-cultures. No specific immunofluorescent or immunogold reactions to monoclonal antibodies (mABs) anti-Frankia < 8C5 > and anti-G. mosseae < F5G5 > were evident in C. equisetifolia callus explants.Key words: arbuscular mycorrhizal fungi (AMF), Frankia, Casuarina, micropropagation, immunofluorescent labelling.

scholarly journals 583 PB 094 CALLUS INDUCTION AND SHOOT ORGANOGENESIS IN RHODODENDRON `BESSE HOWELLS' AND `CATAWBIENSE ALBUM'

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515d-515
Author(s):  
Yiqin Ruan ◽  
Mark Brand
Keyword(s):  
Callus Induction ◽  
Woody Plant ◽  
Shoot Organogenesis ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Indolebutyric Acid ◽  
Callus Production ◽  
Short Shoots ◽  
Number Of Shoots

Combinations of me auxins 2.4-dichlorophenoxyacetic acid (2.4-D). indolebutyric acid (IBA). and naphthaleneacetic acid (NAA), with isopentenylademne (2-iP) were studied in Woody Plant (WP) medium for callus induction and shoot organogenesis from leaves of Rhododendron `Besse Howells' (BH) and `Catawbiense Album' (CA). IBA was more effective than NAA and 2,4-D at inducing shoot organogenesis when combined with 2-iP. Addition of 1 uM IBA and 15 or 30 uM 2-iP to WP medium resulted in the highest percentage of explants producing shoots (90% in BH, 100% in CA), and the greatest number of shoots per explant (18.4 in CA, 10.1 in BH) after 12 weeks of culture. Shoot organogenesis also occurred using 1 uM NAA and 2-iP combinations, but me number of shoots produced was much less than for IBA treatments. 2,4-D and NAA were more productive than IBA for callus induction. Media containing l-10 uM 2,4-D plus 5 uM 2-iP. or 10 uM NAA plus 15 uM 2-iP. were me best for callus production. In studies using thidiazuron instead of 2-iP as the cytokinin, leafy buds and very short shoots developed.

scholarly journals PERBANYAKAN AKASIA HIBRIDA (Acacia mangium × Acacia auriculiformis) MELALUI SUBKULTUR BERULANG

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yelnititis Yelnititis ◽  
Sri Sunarti
Keyword(s):  
Woody Plant ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Acacia Auriculiformis ◽  
Shoot Induction ◽  
Benzyl Adenine ◽  
Single Node ◽  
Woody Plant Medium

In vitro culture is a promising technique for mass propagation of high-value species. Study of propagation for Acacia hybrid (A. mangium x A. auriculiformis) through this technique has been conducted using single node stem from seedlings as explants. Growth medium used was modified Murashige and Skoog (MS), basal medium Woody Plant Medium (WPM), and Gamborg (B5) supplemented. The study was conducted in two stages, namely shoot induction and shoot multiplication. The treatment tested was the Benzyl Adenine (BA) supplementation at the concentration of 0.3; 0.7; and 1.0 mgL-1 of. Observation was conducted on the frequency of shoot induction, number of shoot, shoot length and visual performance of the culture. The result showed that treatment of BA 0.7 mgL-1 on modified MS medium is the best for shoot induction, shoot multiplication and visual performace of the culture. The average of number of shoot was 2.6; 5.0 and 7.7 shoots on the first three consecutive subcultures. Changing to different basal medium on the fourth subculture showed that the treatment of BA 0.7 mgl-1 is the best condition for shoot regeneration (12.60 shoots) and shoot length (6.97 cm). The culture from this treatment showed the best visual morphological performance.Keywords:Acacia hybrid; multiplication; subculture; in vitro; BA. ABSTRAKKultur in vitro merupakan suatu teknik yang menjanjikan untuk perbanyakan massal spesies-tanaman bernilai tinggi. Penelitian perbanyakan akasia hibrid (A. mangium x A. auriculiformis) melalui kulturin vitro telah dilakukan dengan menggunakan eksplan berupa batang satu buku yang berasal dari anakan. Media tumbuh yang digunakan adalah media dasar Murashige dan Skoog (MS) yang sudah dimodifikasi, media dasar Woody Plant Medium (WPM), dan Gamborg (B5). Penelitian dilakukan dalam dua tahap yaitu induksi tunas dan perbanyakan tunas. Perlakuan yang diuji adalah penggunaan Benzyl Adenine (BA) dengan konsentrasi 0,3; 0,7 dan 1,0 mg L-1. Pengamatan dilakukan terhadap waktu induksi tunas, jumlah tunas, tinggi tunas dan penampilan biakan secara visual. Hasil penelitian menunjukkan bahwa penggunaan BA 0,7 mg L-1 pada media MS modifikasi merupakan perlakuan terbaik untuk induksi tunas, perbanyakan tunas, tinggi tunas, dan kondisi biakan secara visual. Jumlah rata-rata tunas yang dihasilkan dari perlakuan ini adalah 2,6; 5,0 dan 7,7 tunas pada subkultur pertama, kedua dan ketiga. Pada penggunaan media dasar berbeda pada subkultur keempat menunjukkan bahwa perlakuan BA 0,7 mg L-1 merupakan perlakuan terbaik dengan jumlah tunas sebanyak 12,60 tunas dan rata-rata tinggi tunas 6,97 cm. Biakan yang dihasilkan dari perlakuan tersebut mempunyai penampilan yang baik dan normal.

scholarly journals Micropropagation of Chokecherry by Shoot Tip Culture

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629c-629
Author(s):  
Zhen Zhang ◽  
Zong-Ming Cheng
Keyword(s):  
Great Plains ◽  
Woody Plant ◽  
Northern Great Plains ◽  
Limiting Factor ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Woody Plant Medium ◽  
Half Strength ◽  
The Media ◽  
Shrubby Species

Chokecherry (Prunus viginiana L.) is an important shrubby species for agroforestry planting in the northern Great Plains states. The X-disease is a serious limiting factor for its utilization. The objective of this research was to produce clonal materials for studying the host and X-disease phytoplasma interactions and for screening X-disease resistant chokecherry germplasms. Shoot tips of 1–2 cm in length were isolated from 1-year old seedling plants, sterilized, and initiated on three basal media supplemented with 5 μm BA and 5 μm IBA. After five weeks, an average of 4.8, 2.2 and 0.3 new shoots were produced on Murashige and Skoog (MS) medium, woody plant medium (WPM) and Knop's medium, respectively. The newly formed shoots were subcultured on MS medium with 5 m BA and 5 m IBA. MS and DKW media gave significantly higher proliferation rates (12–13 shoots after 4 weeks) than WPM (5.5 shoots). Microshoots rooted in half-strength MS medium supplemented with 5 and 10 μm of either IBA or NAA. The shoots were either placed on the medium for 19 days, or for 5 days then transferred to a hormone free medium for 14 days. On the media with IBA, 80% to 90% of the microshoots rooted with an average of 2.4 roots per shoot and there were no differences in rooting percentage and root number. When shoots were exposed to NAA for 5 days, 66.7% of shoots on medium with 5 μm NAA, and 83.3% on the medium with 10 m NAA formed an average of 2.2 roots per shoot; but when the shoots were exposed to NAA for 19 days, 36.4% of shoots on the medium with 5 m NAA and 30% on the medium with 10 μm of NAA formed an average of 0.53 roots per shoot. These rooted shoots are under acclimation to the ambient environment.

scholarly journals In vitro propagation of Xao tam phan (Paramignya trimera (Oliv.) Guill.)

2017 ◽  
Vol 1 (T2) ◽  
pp. 48-57
Author(s):  
Hieu Trung Tran ◽  
Chung Van Huynh ◽  
Hue Thi Linh Bui ◽  
Ngan Thi My Luong ◽  
Anh Lan Bui ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Word Of Mouth ◽  
Woody Plant ◽  
Leaf Explants ◽  
Anticancer Properties ◽  
Best Response ◽  
Woody Plant Medium ◽  
Old Trees ◽  
Cut Surface

Paramignya trimera (Oliv.) Guill., a woody climber commonly known as "Xao tam phan", has been used in Vietnamese folk for the treatment of numerous cancers. Due to word of mouth about the anticancer properties of this plant, its stems and roots have been overexploited leading to the serious decline of this species in Phu Yen, Khanh Hoa and Ninh Thuan provinces. The aim of the study was to establish an in vitro propagation protocol for the conservation of P. trimera. In this research shoot clusters (5–8 shoots/cluster) were regenerated from axillary bud explants of 1–3 year-old trees after 3 months of cultures on the WPM (woody plant medium) supplemented with STS 3 and BA 5–7 mg/L. STS (silver thiosulfate) was used to prevent the leaf abscission. These shoot clusters grew slowly and reached 1–3 cm in heights after 4 months of the cultures. These shoot clusters did not form any roots after 2 months of culture on the rooting media with IBA and/or NAA 1–5 mg/L. However, there was 51 % of the treated shoot clusters acclimatized and produced new stem and leaves after 2 months growing in greenhouse. WPM supplemented with STS 3, BA 5 and IBA 5 mg/L showed the best response for callus induction in leaf explants after 3 months of cultures. Among the callus types, the milky white compact calli were induced at the cut surface of leaf explants after 3 months of the cultures and became the compact and nodulated calli within 4 weeks later.

scholarly journals Production of Anthocyanins in Callus Cultures of Angelica archangelica

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Tomáš Siatka
Keyword(s):  
Woody Plant ◽  
Callus Cultures ◽  
Attractive Alternative ◽  
Dichlorophenoxyacetic Acid ◽  
Angelica Archangelica ◽  
Callus Proliferation ◽  
Order Of Magnitude ◽  
Culture Growth ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Production Characteristics

Anthocyanins have been used as food color additives, but they also possess many properties beneficial to health. Plant tissue culture technology is an attractive alternative for obtaining these valuable natural pigments. In this work, dark-grown anthocyanin producing callus cultures of Angelica archangelica were established. They were cultured on a Murashige and Skoog medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid and 0.4 mg/L benzylaminopurine. Anthocyanin contents in cultures were around 2%, i.e. one order of magnitude higher than in the intact plant that contains up to 0.17% anthocyanins. Growth and production characteristics of the culture were determined – fresh and dry biomass as well as anthocyanin levels reached a maximum on day 30. Effects of basal nutrient media on callus proliferation and anthocyanin accumulation were tested. Culture growth (fresh weight) achieved 105%, 102%, 141%, 129%, 54%, and 26%, and anthocyanin contents attained 114%, 41%, 33%, 31%, 25%, and 15% on Linsmaier and Skoog, Gamborg B5, Schenk and Hildebrandt, Woody plant, Nitsch and Nitsch, and Heller medium, respectively, in comparison with that of Murashige and Skoog.

INDUCTION AND MAINTENANCE OF EMBRYOGENIC CHARACTERISTICS OF CALLUS OF THE OIL PALM HYBRID MANICORÉ

2021 ◽  
Vol 45 ◽  
Author(s):  
Marlúcia Souza Pádua Vilela ◽  
Jéssica de Castro e Andrade ◽  
Raíssa Silveira Santos ◽  
Vanessa Cristina Stein ◽  
Patrick Callegari Magnani Santos Alves ◽  
...  
Keyword(s):  
Oil Palm ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Callus Formation ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
In Vitro Cultivation ◽  
Low Concentrations ◽  
2,4 Dichlorophenoxyacetic Acid

ABSTRACT Large-scale oil palm propagation (Elaeis guineensis Jacq.) is difficult due to its unique apical meristem. In this context, micropropagation allows the multiplication of seedlings in vitro and the storage of germplasm elites. This study aimed to induce embryogenic calluses from leaves of oil palm plants in low concentrations of auxins and to observe the maintenance of these characteristics during in vitro cultivation. Calluses were induced in 0.5 cm leaf explants in Y3 culture medium supplemented with Picloram (4-Amino-3,5,6-trichloro-2-pyridinecarboxylic acid) or 2,4-D (2,4-dichlorophenoxyacetic acid), at concentrations of 0, 1, 3, 6, and 9 mg L-1. The callus with embryogenic appearance was subcultured and evaluated regarding maintenance of embryogenic characteristics by cytochemical analyses. The best treatment for induction of calluses was composed of 1mg.L-1 of Picloram, which led to 30% callus formation. The calluses were classified into4 types, based on color and morphology. The cells of calluses with nodular and beige appearance have embryogenic characteristics, and the embryogenic potential of the cell masses was maintained over the 20 months of cultivation. This differentiated adaptation to the protocol can allow the advance in the mass propagation of oil palm through tissue culture, indicating the importance of investigating the topics proposed by the research.

SOMATIC EMBRYOGENESIS IN CELL SUSPENSION CULTURE OF COWPEA (VIGNA UNGUICULATA (L.) WALP)

1995 ◽  
Vol 43 (4) ◽  
pp. 385-390 ◽  
Author(s):  
S. Kulothungan ◽  
A. Ganapathi ◽  
A. Shajahan ◽  
K. Kathiravan
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Vigna Unguiculata ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Seedling Leaf ◽  
Cotyledonary Stage

Embryogenic callus was induced from seedling leaf explants of cowpea (Vigna unguiculata (L.) Walp. cv. C152 on Murashige and Skoog (MS) medium containing 2.0 mg 1−1 2,4-dichlorophenoxyacetic acid (2,4-D). The maximum frequency of somatic embryogenesis was noticed when this callus was transferred to MS liquid medium supplemented with 2 mg 1−1 2,4-D. Further studies on ontogeny of somatic embryos showed that the cells destined to become somatic embryos divided into spherical or filamentous proembryos. Subsequent divisions in the proembryo led to globular, heart, torpedo-shaped, and cotyledonary-stage somatic embryos. Tiny plantlets were obtained by transferring the cotyledonary-stage somatic embryos to MS liquid medium containing 0.5 mg 1−1 2,4-D.

The occurrence of polar structures in callus cultures from mature lodgepole pine (Pinus contorta var. latifolia)

1988 ◽  
Vol 66 (12) ◽  
pp. 2595-2596
Author(s):  
Susan C. MacDougall ◽  
Shona M. Ellis ◽  
Iain E. P. Taylor
Keyword(s):  
Pinus Contorta ◽  
Zygotic Embryo ◽  
Lodgepole Pine ◽  
Leaf Explants ◽  
Callus Cultures ◽  
Small Cells ◽  
Dichlorophenoxyacetic Acid ◽  
Pine Trees ◽  
Embryo Cells ◽  
2,4 Dichlorophenoxyacetic Acid

A somatic polar structure was observed in white callus cultured, in the presence of 2,4-dichlorophenoxyacetic acid (10−6 M) and benzylaminopurine (4 × 10−6 M), from leaf explants taken from mature lodgepole pine trees. The structure contained elongate, vacuolate cells and small cells arranged with some resemblance to the first zygotic embryo cells. We were not able to induce further development.

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