Preliminary histological investigations of the defense reactions of three pines to Ceratocystisclavigera and two chemical elicitors

1988 ◽  
Vol 18 (10) ◽  
pp. 1243-1247 ◽  
Author(s):  
François Lieutier ◽  
Alan A. Berryman
Keyword(s):  
Proteinase Inhibitor ◽  
Growth Rings ◽  
Sieve Cells ◽  
Cupric Acetate ◽  
Defense Reactions ◽  
Parenchyma Cells ◽  
Pine Trees ◽  
Potential Mode ◽  
Buffer Control ◽  
Chitosan Resin

The histology of defense reactions in individual pine trees belonging to three species, Pinusponderosa, P. contorta, and P. monticola, to inoculation with Ceratocystisclavigera and injection with chitosan and a proteinase inhibitor inducing factor was investigated after resin fixation with cupric acetate. The synthesis of secondary resins was directly associated with parenchyma cells in the phloem and in the rays of both phloem and sapwood. However, these resins were largely located in the lumen of sieve cells and tracheids. In the case of the fungus and chitosan, resin soaking began in the outer phloem and latewood of the last growth rings, then spread into the inner phloem and earlywood. With the proteinase inhibitor inducing factor, resinosis was similar to that of the buffer control, being much less extensive than with the fungus or chitosan, and occurring mainly in the inner phloem and earlywood. In P. monticola, the reaction was complicated by the appearance of fissures in the phloem, probably caused by tension from resin pressure. Phloem resinosis was accompanied by apparent secretion of phenolic compounds, presumably from parenchyma cells. The possible origin of these secondary resins and the potential mode of action of the chemicals used are discussed.

Anatomy and Chemical Composition of Pinus Pinaster Bark

IAWA Journal ◽  
1996 ◽  
Vol 17 (2) ◽  
pp. 141-150 ◽  
Author(s):  
Elsa Nunes ◽  
Teresa Quilhó ◽  
Helena Pereira
Keyword(s):  
Chemical Composition ◽  
Pinus Pinaster ◽  
Variable Number ◽  
Starch Granules ◽  
Secondary Phloem ◽  
Scale Type ◽  
Resin Ducts ◽  
Sieve Cells ◽  
Parenchyma Cells

The secondary phloem of Pinus pinaster Aiton bark has sieve cells and axial and radial parenchyma, but no fibres. Resin ducts are present in fusiform rays . Stiloid crystals, starch granules and tannins occur inside sieve and parenchyma cells. The rhytidome of P. pinaster bark has a variable number of periderms forming scale-type discontinuous layers over expanded parenchyma cells. Phellem comprises 4-6 layers of thickwaIled and little suberized cells and phelloderm a layer of 2 or 3 thickened lignified ceIls and a layer of expanded cells.

The secondary phloem of Austrobaileya scandens

1970 ◽  
Vol 48 (2) ◽  
pp. 341-359 ◽  
Author(s):  
Lalit M. Srivastava
Keyword(s):  
Tannic Acid ◽  
Cross Sections ◽  
Significant Proportion ◽  
Sieve Tube ◽  
Secondary Phloem ◽  
Sieve Elements ◽  
Sieve Cells ◽  
Companion Cells ◽  
Parenchyma Cells ◽  
Definition Of

The origin of sieve elements and parenchyma cells in the secondary phloem of Austrobaileya was studied by use of serial cross sections stained with tannic acid – ferric chloride and lacmoid. In three important respects, Austrobaileya phloem recalls gymnospermous features: it has sieve cells rather than sieve-tube members; a significant proportion of sieve elements and companion cells arise independently of each other; and sieve areas occur between sieve elements and companion cells ontogenetically unrelated to each other. The angiospermous feature includes origin of most sieve elements and parenchyma, including companion cells, after divisions in phloic initials. In these instances companion cells show a closer ontogenetic relationship to sieve elements than do other parenchyma cells. The combination of gymnospermous and angiospermous features makes phloem of Austrobaileya unique when compared to that of all those species that have been investigated in detail. It is further suggested that the term albuminous cells is inappropriate and should be replaced by companion cells but that the ontogenetic relationship implicit in the definition of companion cells is too restrictive and should be abandoned.

scholarly journals Effects of pruning on heartwood formation in Scots pine trees

2012 ◽  
Vol 50 (No. 1) ◽  
pp. 11-16
Author(s):  
B. Bergström ◽  
R. Gref ◽  
A. Ericsson
Keyword(s):  
Growth Rate ◽  
Pinus Sylvestris ◽  
Scots Pine ◽  
Heartwood Formation ◽  
Growth Rings ◽  
Significant Negative Effect ◽  
Growing Seasons ◽  
Pine Trees ◽  
Negative Effect ◽  
Proportional Increase

The object of this study was to investigate the effect of pruning on heartwood formation in mature Scots pine (Pinus sylvestris L.) trees. Fifty trees were treated by three different intensive pruning regimes: 42, 60 and 70 percentage of defoliation. After five growing seasons numbers of growth rings were counted and the width and the area of sapwood and heartwood were calculated. The results did not show any proportional increase or decrease in the heartwood area or in the number of growth rings in heartwood associated with the pruning. A statistically significant negative effect of pruning was found on the width of the five most recently formed sapwood growth rings. This decreased growth rate did not influence the ratio of sapwood and heartwood. However, it cannot be excluded that the proportion of heartwood may increase during a longer period. It is concluded that pruning is not a practicable silvicultural method for regulating heartwood formation in mature Scots pine trees.

scholarly journals Conducting tissues and phyletic relationships of bryophytes

2000 ◽  
Vol 355 (1398) ◽  
pp. 795-813 ◽  
Author(s):  
R. Ligrone ◽  
J. G. Duckett ◽  
K. S. Renzaglia
Keyword(s):  
Long Distance ◽  
Sieve Cells ◽  
Parenchyma Cells ◽  
Cytoplasmic Organization ◽  
Symplasmic Transport ◽  
Axial System ◽  
Similar Organization

Internal specialized conducting tissues, if present, are restricted to the gametophytic generation in liverworts while they may occur in both generations in mosses. Conducting tissues are unknown in the anthocerotes. Water–conducting cells (WCCs) with walls perforated by plasmodesma–derived pores occur in the Calobryales and Pallaviciniaceae (Metzgeriales) among liverworts and in Takakia among mosses. Imperforate WCCs (hydroids) are present in bryoid mosses. A polarized cytoplasmic organization and a distinctive axial system of microtubules is present in the highly specialized food–conducting cells of polytrichaceous mosses (leptoids) and in less specialized parenchyma cells of the leafy stem and seta in other mosses including Sphagnum . A similar organization, suggested to reflect specialization in long–distance symplasmic transport of nutrients, also occurs in other parts of the plant in mosses, including rhizoids and caulonemata, and may be observed in thallus parenchyma cells of liverworts. Perforate WCCs in the Calobryales, Metzgeriales and Takakia , and hydroids in bryoid mosses, probably evolved independently. Because of fundamental differences in developmental design, homology of any of these cells with tracheids is highly unlikely. Likewise, putative food–conducting of bryophytes present highly distinctive characteristics and cannot be considered homologous with the sieve cells of tracheophytes.

scholarly journals Localization of (+)-Catechin in Picea abies Phloem: Responses to Wounding and Fungal Inoculation

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2952
Author(s):  
Tuula Jyske ◽  
Katsushi Kuroda ◽  
Susanna Keriö ◽  
Andrey Pranovich ◽  
Riikka Linnakoski ◽  
...  
Keyword(s):  
Norway Spruce ◽  
Defense Mechanisms ◽  
Resin Acid ◽  
Tree Bark ◽  
Chemical Mapping ◽  
Phloem Parenchyma ◽  
Tof Sims ◽  
Defense Reactions ◽  
Parenchyma Cells ◽  
Fungal Inoculation

To understand the positional and temporal defense mechanisms of coniferous tree bark at the tissue and cellular levels, the phloem topochemistry and structural properties were examined after artificially induced bark defense reactions. Wounding and fungal inoculation with Endoconidiophora polonica of spruce bark were carried out, and phloem tissues were frequently collected to follow the temporal and spatial progress of chemical and structural responses. The changes in (+)-catechin, (−)-epicatechin, stilbene glucoside, and resin acid distribution, and accumulation patterns within the phloem, were mapped using time-of-flight secondary ion mass spectrometry (cryo-ToF-SIMS), alongside detailed structural (LM, TEM, SEM) and quantitative chemical microanalyses of the tissues. Our results show that axial phloem parenchyma cells of Norway spruce contain (+)-catechins, the amount of which locally increases in response to fungal inoculation. The preformed, constitutive distribution and accumulation patterns of (+)-catechins closely follow those of stilbene glucosides. Phloem phenolics are not translocated but form a layered defense barrier with oleoresin compounds in response to pathogen attack. Our results suggest that axial phloem parenchyma cells are the primary location for (+)-catechin storage and synthesis in Norway spruce phloem. Chemical mapping of bark defensive metabolites by cryo-ToF-SIMS, in addition to structural and chemical microanalyses of the defense reactions, can provide novel information on the local amplitudes and localizations of chemical and structural defense mechanisms and pathogen–host interactions of trees.

WOOD ANATOMY OF MYRCIARIA, NEOMITRANTHES, PLINIA AND SIPHONEUGENA SPECIES (MYRTEAE, MYRTACEAE)

IAWA Journal ◽  
2013 ◽  
Vol 34 (3) ◽  
pp. 313-323 ◽  
Author(s):  
Gabriel U.C.A. Santos ◽  
Cátia H. Callado ◽  
Marcelo da Costa Souza ◽  
Cecilia G. Costa
Keyword(s):  
Wood Anatomy ◽  
Growth Rings ◽  
Anatomical Features ◽  
Bordered Pits ◽  
Parenchyma Cells ◽  
Ray Parenchyma ◽  
Square Cells ◽  
Ray Parenchyma Cells ◽  
Perforation Plates ◽  
Fruit Characters

Myrciaria, Neomitranthes, Plinia and Siphoneugena are closely related genera whose circumscriptions are controversial. The distinctions between Myrciaria vs. Plinia, and Neomitranthes vs. Siphoneugena, have been based on a few fruit characters. The wood anatomy of 24 species of these genera was examined to determine if wood anatomical features could help delimit the genera. It was determined the four genera cannot reliably be separated by wood anatomy alone. Characteristics seen in all four genera are: growth rings usually poorly-defined; diffuse porous; exclusively solitary vessels, usually circular to oval in outline; simple perforation plates; vessel-ray pits alternate and distinctly bordered; fibers with distinctly bordered pits in radial and tangential walls, usually very thickwalled; vasicentric tracheids typically absent; scanty paratracheal parenchyma, sometimes unilateral, and diffuse to diffuse-in-aggregates; chambered crystalliferous axial parenchyma in many species, usually both prismatic and smaller crystals; rays 1–4-seriate, uniseriate rays composed of upright/square cells, multiseriate rays with procumbent body cells and 1 to many marginal rows of upright/square cells; disjunctive ray parenchyma cells usually present.

Specialized phloem parenchyma cells in Norway spruce (Pinaceae) bark are an important site of defense reactions

1998 ◽  
Vol 85 (5) ◽  
pp. 601-615 ◽  
Author(s):  
Vincent R. Franceschi ◽  
Trygve Krekling ◽  
Alan A. Berryman ◽  
Erik Christiansen
Keyword(s):  
Norway Spruce ◽  
Phloem Parenchyma ◽  
Defense Reactions ◽  
Parenchyma Cells ◽  
Important Site

Vertical and Radial Variation of Nuclear Elongation Index of Living Sapwood Ray Parenchyma Cells in a Plantation Tree of Cryptomeria Japonica

IAWA Journal ◽  
1994 ◽  
Vol 15 (3) ◽  
pp. 323-327 ◽  
Author(s):  
K.C. Yang ◽  
Y.S. Chen ◽  
C.A. Benson
Keyword(s):  
Metabolic Activity ◽  
Cryptomeria Japonica ◽  
Ground Level ◽  
Tree Crown ◽  
Growth Rings ◽  
Parenchyma Cells ◽  
Ray Parenchyma ◽  
Ray Cells ◽  
Elongation Index ◽  
Ray Parenchyma Cells

Vertical and radial variations of nuclear elongation index (NEI) of living sapwood ray parenchyrna cells were studied in a 45-year-old plantation tree of Cryptomeria japonica D. Don collected in Taiwan on February 27, 1992. Nine wood strips oriented in an E-W direction of the tree were collected starting at 0.3 m above ground level, and progressing upwards by 2.5 m intervals to the tree crown. Radial sections, 20 µm thick, were cut from the cambium toward the inner sapwood of these nine wood strips. The nuclear elongation index (NEI) was used to express the metabolic activity of the ray cells. It was found that metabolic activity of sapwood ray parenchyma was thc highest at the outer sapwood and declined gradually towards the inner sapwood. The lowest average NEI was found at the lowest stern level. The average NEI of various stern height levels increased with increasing stern height level. The average NEI of three growth rings at the outer sapwood near the cambium reached a maximum at the bottom of the live crown.

DISTRIBUTION AND VITALITY OF XYLEM RAYS IN RELATION TO TREE LEAF AREA IN DOUGLAS-FIR

IAWA Journal ◽  
2000 ◽  
Vol 21 (4) ◽  
pp. 389-401 ◽  
Author(s):  
Barbara L. Gartner ◽  
David C. Baker ◽  
Rachel Spicer
Keyword(s):  
Leaf Area ◽  
Pseudotsuga Menziesii ◽  
Douglas Fir ◽  
Relative Volume ◽  
Growth Rings ◽  
Breast Height ◽  
Parenchyma Cells ◽  
Ray Parenchyma ◽  
Ray Parenchyma Cells ◽  
Tree Leaf

The factors that determine sapwood width and volume in a tree are not known. This study asked whether sapwood width is related to a need for stem storage sites. Experiments were conducted on 12 34-year-old Douglas-fir [(Pseudotsuga menziesii (Mirb.) Franco] trees with a 6-7 fold range of leaf areas and leaf area/sapwood volumes. Because of declining ray frequency but constant average ray area, ray volume declined for the first 6-10 growth rings, then remained constant, and did not vary with height (breast height vs. 10 nodes from the top). Fewer of the ray parenchyma cells had nuclei in inner than outer sapwood. Inner sapwood had ray parenchyma with smaller rounder nuclei than did outer sapwood, and there was no effect of height. There was a positive relationship between leaf area and the relative volume of ray in outer sapwood at breast height (r = 0.646, p = 0.02), supporting the hypothesis that Douglas-fir trees with larger leaf areas have higher ray volume than do trees with smaller leaf areas. However, correlations of leaf area I sapwood volume with leaf area at either height were not significant, nor were correlations of either leaf area or leaf area/sapwood volume with measures of ray vitality (nuclear frequency in outer sapwood, or the ratio of nuclear frequency in the middle I outer sapwood or in inner I outer sapwood). These latter correlations give no evidence that Douglas-fir trees determine their sapwood volume based on a need for quantity of vital xylem rays.

scholarly journals Anatomical features of bald cypress - exotic for meliorative afforestation

2019 ◽  
pp. 61-76
Author(s):  
Dusan Jokanovic ◽  
Nikolic Jokanovic ◽  
Aleksandar Andjelkovic ◽  
Katarina Lazarevic ◽  
Radoslav Lozjanin
Keyword(s):  
Growth Ring ◽  
Simple Equation ◽  
Stem Height ◽  
Growth Rings ◽  
Anatomical Features ◽  
Bald Cypress ◽  
Cambial Age ◽  
Parenchyma Cells

The paper deals with height and density of woody rays per mm2 by bald cypress stems at two alluvial sites in Serbia (Veliko ratno ostrvo and Backa Palanka). Overall 6 stems (3 at one and 3 at another locality) were harvested. After that discs were made and they served for permanent anatomical preparations making. All necessary measurements were performed on these preparations. Inside each growth ring was selected 6 visible fields (3 at early- and 3 at latewood zone) and within all fields density of woody rays per mm2 was calculated. According to simple equation number of woody rays per mm2 was determined. As for height of woody rays, it was calculated by parenchyma cells counting. Researched bald cypress features were measured depending on 3 factors: cambial age, stem height and zone inside growth ring. As for relation between number and height of woody rays, there is obvious inverse - greater density of woody rays means they are shorter. The scope of the paper was to establish how height and number of woody rays per mm2 change depending on 3 observed factors - age, stem height and zone inside growth rings.

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