Use of a video-imaging system for estimating leaf surface area of Pinussylvestris seedlings

1988 ◽  
Vol 18 (3) ◽  
pp. 377-380 ◽  
Author(s):  
Kent S. Diebolt ◽  
Kenneth W. Mudge
Keyword(s):  
Surface Area ◽  
Imaging System ◽  
Dry Weight ◽  
Video Camera ◽  
Image Analyzer ◽  
Pine Seedlings ◽  
Weight Calculation ◽  
Leaf Surface Area ◽  
Silhouette Area ◽  
Nondestructive Estimation

A method is described for a rapid, nondestructive estimation of the surface area of the shoot system of intact pine seedlings. A video camera and image analyzer were used to quantify the area of a video image of pine seedling shoots. Surface area values were displayed in dimensionless units, referred to as silhouette area. Silhouette area was significantly correlated with several other methods of estimating needle surface area, including dry weight, calculation based on measurement of linear dimensions, volume displacement, and optical planimetry. Correspondence between dry weight, or surface area estimates based on dry weight, and silhouette area was relatively low (r2 < 0.9) but statistically significant. The correlation between shoot area and silhouette area were improved (r2 > 0.9) when shoot area was estimated from volume displacement or optical planimetry.

scholarly journals Scaling Relationships between Leaf Shape and Area of 12 Rosaceae Species

Symmetry ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1255 ◽  
Author(s):  
Xiaojing Yu ◽  
Cang Hui ◽  
Hardev S. Sandhu ◽  
Zhiyi Lin ◽  
Peijian Shi
Keyword(s):  
Surface Area ◽  
Leaf Surface ◽  
Leaf Shape ◽  
Dry Weight ◽  
Leaf Width ◽  
Scaling Exponent ◽  
Rosaceae Species ◽  
Scaling Relationship ◽  
Leaf Surface Area ◽  
Leaf Dry Weight

Leaf surface area (A) and leaf shape have been demonstrated to be closely correlated with photosynthetic rates. The scaling relationship between leaf biomass (both dry weight and fresh weight) and A has been widely studied. However, few studies have focused on the scaling relationship between leaf shape and A. Here, using more than 3600 leaves from 12 Rosaceae species, we examined the relationships of the leaf-shape indices including the left to right side leaf surface area ratio (AR), the ratio of leaf perimeter to leaf surface area (RPA), and the ratio of leaf width to length (RWL) versus A. We also tested whether there is a scaling relationship between leaf dry weight and A, and between PRA and A. There was no significant correlation between AR and A for each of the 12 species. Leaf area was also found to be independent of RWL because leaf width remained proportional to leaf length across the 12 species. However, there was a negative correlation between RPA and A. The scaling relationship between RPA and A held for each species, and the estimated scaling exponent of RPA versus A approached −1/2; the scaling relationship between leaf dry weight and A also held for each species, and 11 out of the 12 estimated scaling exponents of leaf dry weight versus A were greater than unity. Our results indicated that leaf surface area has a strong scaling relationship with leaf perimeter and also with leaf dry weight but has no relationship with leaf symmetry or RWL. Additionally, our results showed that leaf dry weight per unit area, which is usually associated with the photosynthetic capacity of plants, increases with an increasing A because the scaling exponent of leaf dry weight versus A is greater than unity. This suggests that a large leaf surface area requires more dry mass input to support the physical structure of the leaf.

Growth and water relations of black locust and pine seedlings exposed to controlled water stress

1983 ◽  
Vol 13 (2) ◽  
pp. 334-338 ◽  
Author(s):  
Paul J. Schulte ◽  
Paul E. Marshall
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Surface Area ◽  
Black Locust ◽  
Dry Weight ◽  
Growth Conditions ◽  
Red Pine ◽  
Polyethylene Glycol 4000 ◽  
Xylem Pressure ◽  
Pine Seedlings

Some responses of very young jack pine (Pinusbanksiana Lamb.), red pine (Pinusresinosa Ait.), and black locust (Robiniapseudoacacia L.) seedlings grown under constant water stress conditions induced by polyethylene glycol 4000 culture solutions were investigated. Seedlings grown under stressful conditions had lower leaf conductance, produced less foliar surface area, and accumulated less dry weight than nonstressed seedlings. Seedlings grown under nonstressed conditions were found to undergo considerable transpiration at night. When compared with the pines, black locust seedlings grew more rapidly, had lower (more negative) xylem pressure potentials, and showed poorer survival under the more stressful growth conditions.

scholarly journals Drought Avoidance in Katsura by Drought-induced Leaf Abscission and Rapid Refoliation

HortScience ◽  
1999 ◽  
Vol 34 (5) ◽  
pp. 871-874 ◽  
Author(s):  
Michael S. Dosmann ◽  
William R. Graves ◽  
Jeffery K. Iles
Keyword(s):  
Surface Area ◽  
Leaf Surface ◽  
Weight Ratio ◽  
Dry Weight ◽  
Leaf Abscission ◽  
Control Treatment ◽  
Drought Treatment ◽  
Temporary Reduction ◽  
Leaf Surface Area ◽  
Leaf Dry Weight

The limited use of the katsura tree (Cercidiphyllum japonicum Sieb. & Zucc.) in the landscape may be due to its reputed, but uncharacterized, intolerance of drought. We examined the responses of katsura trees subjected to episodes of drought. Container-grown trees in a greenhouse were subjected to one of three irrigation treatments, each composed of four irrigation phases. Control plants were maintained under well-hydrated conditions in each phase. Plants in the multiple-drought treatment were subjected to two drought phases, each followed by a hydration phase. Plants in the single-drought treatment were exposed to an initial drought phase followed by three hydration phases. Trees avoided drought stress by drought-induced leaf abscission. Plants in the multiple- and single-drought treatments underwent a 63% and 34% reduction in leaf dry weight and a 60% and 31% reduction in leaf surface area, respectively. After leaf abscission, trees in the single-drought treatment recovered 112% of the lost leaf dry weight within 24 days. Leaf abscission and subsequent refoliation resulted in a temporary reduction in the leaf surface area: root dry weight ratio. After relief from drought, net assimilation rate and relative growth rate were maintained at least at the rates associated with plants in the control treatment. We conclude that katsura is a drought avoider that abscises leaves to reduce transpirational water loss. Although plants are capable of refoliation after water becomes available, to maintain the greatest ornamental value in the landscape, siting of katsura should be limited to areas not prone to drought.

Seasonal changes in biomass and shoot characteristics of a Zostera capricorni Aschers. Dominant meadow in Cairns Harbour, northern Queensland

1994 ◽  
Vol 45 (7) ◽  
pp. 1337 ◽  
Author(s):  
LJ McKenzie
Keyword(s):  
Surface Area ◽  
Seasonal Pattern ◽  
Light Availability ◽  
Environmental Parameters ◽  
Dry Weight ◽  
Spatial And Temporal Variability ◽  
Above Ground Biomass ◽  
Ground Biomass ◽  
Leaf Surface Area ◽  
Below Ground

Spatial and temporal variability of Z. capricorni biomass, shoot characteristics (canopy height, surface area, flowering), distribution and detrital content were examined from December 1988 to December 1990. Between August 1987 and August 1991, 15% (2.0 ha) of the meadow was lost. Biomass of above- and below-ground structures showed a unimodal seasonal pattern with maxima in late spring (mean 194.92 g dry weight m-2 and 426.67 g DW m-2 respectively) and minima in winter (mean 28.72 g DW m-2 and 56.98 g DW m-2 respectively). Mean above-ground biomass (95.53 � 2.21 g DW m-2) was approximately half the mean below-ground biomass (177.28 � 4.49 g DW m-2). Leaf canopy heights were greatest between October and February (maximum 53.4 cm) and lowest around mid year (minimum 4.4 cm). Leaf surface area per square metre of seagrass meadow ranged from 10.28 to 1.39 m2 (mean 3,692 � 0.104 m2), and flowering occurred during September and October. Detrital biomass ranged from 339.73g DW m-2 to 11.83 g DW m-2 (mean 77.39 � 2.36 g DW m-2). Detrital biomass was higher during July-October and lower during February-May. The climate during the study was typical for the area, and all trials displayed similar seasonal patterns, although the amplitudes differed among some trials. The environmental parameters that may influence seagrass and detrital biomass were investigated. The best models explained only 14% of the variation in above-ground biomass, 15% of the variation in below-ground biomass, and 21% of the variation in detrital biomass. These models suggest that fluctuations in seagrass and detrital biomass in Cairns Harbour were influenced by changes in light availability, temperature, salinity and exposure.

scholarly journals Cultural Treatment and Cutting Type Affects Growth of ‘Heritage’ Birch Rooted Cuttings

1985 ◽  
Vol 3 (3) ◽  
pp. 142-146
Author(s):  
Daniel K. Struve
Keyword(s):  
Surface Area ◽  
Leaf Surface ◽  
Dry Weight ◽  
Rooted Cuttings ◽  
Relative Growth Rates ◽  
Leaf Surface Area ◽  
Long Shoots ◽  
Net Assimilation ◽  
Total Dry Weight ◽  
Short Shoots

Rooted cuttings of Betula nigra ‘Heritage’ originating from short shoots were either staked and lateral shoots pruned to a 3 node length or were unstaked and unpruned. One hundred and twenty-five days following propagation, the pruned and staked plants had higher net assimilation rates and lower leaf area ratios than unstaked and unpruned plants. No differences in relative growth rates, total dry weight, root/shoot ratios or shoot length were observed between control and treated plants. Pruned and staked plants had, relative to unstaked and unpruned, less leaf surface area composed of fewer but larger leaves which produced more grams of dry weight per cm2 leaf surface area per day. Pruning and staking maximized economically important dry matter production without reducing total dry weight. Plants originating from long shoots had greater root, shoot and total dry weights, and required less staking to produce upright growth than plants originating from short shoots. By exploiting the beneficial topophysis growth of long shoots, nursery productivity can be increased.

scholarly journals The nutmeg seedlings growth under pot culture with biofertilizers inoculation

2021 ◽  
Vol 6 (1) ◽  
pp. 1-10
Author(s):  
Reginawanti Hindersah ◽  
Agusthinus Marthin Kalay ◽  
Henry Kesaulya ◽  
Cucu Suherman
Keyword(s):  
Surface Area ◽  
Plant Height ◽  
Leaf Surface ◽  
Block Design ◽  
Foliar Spray ◽  
Dry Weight ◽  
Low Fertility ◽  
Soil Application ◽  
Leaf Surface Area ◽  
Phosphate Solubilizing

Abstract Nutmeg is important for national and community revenue mainly in Maluku Province where nutmeg seedlings are grown in low-fertility soil without fertilizer. A greenhouse experiment was performed to evaluate the response of nutmeg seedlings following the application of two different biofertilizer concortia. The experimental design was completely randomized block design, which tested the combination treatments of two rates and the application methods of biofertilizer concortium. The rates of “bacillus biofertilizer” was 0.15 and 0.3%, while the rates of “mixed biofertilizer” was 0.5 and 1.0%. Both biofertilizer were inoculated by foliar spray and soil application. The results verified that at 24 weeks after inoculation, biofertilizers increased the seedling growth traits which included plant height, shoot dry weight, leaf surface area, root number, and root dry weight over the control. Soil application by 1% of “mixed biofertilizer” consists of nitrogen-fixing bacteria and phosphate-solubilizing microbes resulted in better seedlings performance. However, the highest plant height was demonstrated by seedlings treated with 0.3% “bacillus biofertilizer” composed of phosphate solubilizing Bacillus. Biofertilizer inoculation also enhanced soil microbes and leaf surface area but did not change the root-to-shoot ratio of the seedlings. The results showed that biofertilizer inoculation improves the growth of nutmeg seedlings.

scholarly journals The formation of the leaf surface area and biomass of the miscanthus giganteus plants depending on the sewage sludge rate

2021 ◽  
Vol 280 ◽  
pp. 06009
Author(s):  
Vasyl Lopushniak ◽  
Нalyna Hrytsuliak ◽  
Mykhailo Gumentyk ◽  
Mykola Kharytonov ◽  
Bazena Barchak ◽  
...  
Keyword(s):  
Sewage Sludge ◽  
Surface Area ◽  
Leaf Surface ◽  
Net Photosynthesis ◽  
Dry Weight ◽  
Dry Mass ◽  
Podzolic Soils ◽  
Miscanthus Giganteus ◽  
Leaf Surface Area

The case study to determine the peculiarities of Miscanthus giganteus aboveground biomass formation depending on sewage sludge and composts rate carried out in the Precarpathian region of Ivano- Frankivsk province on sod-podzolic soils. The largest area of the leaf surface of miscanthus is formed in the trials where fresh sewage sludge was applied in the rate of 20 - 40 t/ha. The leaf surface area increases from 19 up to 24.0 cm2/plant, and the yield of raw mass of plants at the level of 23.5 - 25.1 t/ha due to increasing rates of sewage sludge application. The highest indicators of net photosynthesis productivity were found in the period of intensive growth, which amounted to 7.78 g/m²/day and in the maturation period of 7.56 g/m²/day in the trial SS - 40 t/ha + N10P14K58. The amount of dry mass of miscanthus plants significantly depends on the height of the shoot and the leaf surface area of the plants. The use of compost based on sewage sludge and straw in a ratio of 3: 1 at a rate of 30 t/ha contributes to the dry weight of miscanthus plants at the level of 15 t/ha.

The versatility of the light microscope image analyzer

1993 ◽  
Vol 51 ◽  
pp. 218-219
Author(s):  
E.L. Cardell ◽  
R.R. Cardell
Keyword(s):  
Electron Microscope ◽  
High Resolution ◽  
Computer Image ◽  
Light Microscope ◽  
Imaging System ◽  
Video Camera ◽  
Color Images ◽  
Image Analyzer ◽  
Microscope Image ◽  
Color Video

The centralization of resources means equipment and technical staff must be versatile since their services will be used by various investigators evaluating different applications and tissues. The Nikon Microphot light microscope (LM) equipped with MTI video camera, Gateway 2000 computer, Image-1 imaging system, and Sony Color Video Printer is helpful for the evaluation of sections of tissue from different projects, most of which are also electron microscope studies that benefit from LM analysis. The high resolution prints are immediately available to the researcher. The various computer manipulations facilitate the visualization of routinely stained sections and sections after autoradiography, β- galactosidase localization, and immunogold-silver cytochemistry, etc. Monochrome or color prints are possible with the display modes provided, or display modes may be edited as beneficial for a particular application. We have designed display modes that allow monochrome and color images in the same print. NIH Grant DK27097.

Correlation of Pore Structure and Permeability by SEM-Image Analysis

1990 ◽  
Vol 48 (1) ◽  
pp. 428-429
Author(s):  
C. A. Callender ◽  
Wm. C. Dawson ◽  
J. J. Funk
Keyword(s):  
Image Analysis ◽  
Pore Structure ◽  
Imaging System ◽  
Pore Space ◽  
Simulation Models ◽  
Image Analyzer ◽  
Imaging Data ◽  
Sem Images ◽  
Thin Sections ◽  
Rock Types

The geometric structure of pore space in some carbonate rocks can be correlated with petrophysical measurements by quantitatively analyzing binaries generated from SEM images. Reservoirs with similar porosities can have markedly different permeabilities. Image analysis identifies which characteristics of a rock are responsible for the permeability differences. Imaging data can explain unusual fluid flow patterns which, in turn, can improve production simulation models.Analytical SchemeOur sample suite consists of 30 Middle East carbonates having porosities ranging from 21 to 28% and permeabilities from 92 to 2153 md. Engineering tests reveal the lack of a consistent (predictable) relationship between porosity and permeability (Fig. 1). Finely polished thin sections were studied petrographically to determine rock texture. The studied thin sections represent four petrographically distinct carbonate rock types ranging from compacted, poorly-sorted, dolomitized, intraclastic grainstones to well-sorted, foraminiferal,ooid, peloidal grainstones. The samples were analyzed for pore structure by a Tracor Northern 5500 IPP 5B/80 image analyzer and a 80386 microprocessor-based imaging system. Between 30 and 50 SEM-generated backscattered electron images (frames) were collected per thin section. Binaries were created from the gray level that represents the pore space. Calculated values were averaged and the data analyzed to determine which geological pore structure characteristics actually affect permeability.

SOOTY MOULD DISEASES OF SOME TREES FROM AURANGABAD DISTRICT, MAHARASHTRA (INDIA)

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
NAVALSINGH J. TODAWAT
Keyword(s):  
Surface Area ◽  
Fungal Pathogens ◽  
Leaf Surface ◽  
Severity Of Disease ◽  
Sooty Mould ◽  
Leaf Surface Area

Sooty mould diseases of Tress from Aurangabad district were surveyed. During the survey of tress, 5 species were found infected by fungal pathogens causing sooty mould diseases. Disease is easily identifiable by the presence of a black, velvety growth covering the leaf surface area. The fungus produces mycelium which is superficial and dark grows on the flowers, leaf, stem and sometime on fruits also. The severity of disease depends on the honeydew secretions by insects. The diseases were found to be caused by 5 species of fungi viz. Capnodium anonae, C. ramosum, Capnodium sp., Meliola bangalorensis and Meliola ranganthii.

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