Genetic mapping of linkage group XIX and identification of sex-linked SSR markers in a Populus tremula × Populus tremuloides cross

2011 ◽  
Vol 41 (2) ◽  
pp. 245-253 ◽  
Author(s):  
Birte Pakull ◽  
Katrin Groppe ◽  
Federica Mecucci ◽  
Muriel Gaudet ◽  
Maurizio Sabatti ◽  
...  
Keyword(s):  
Linkage Group ◽  
Genetic Mapping ◽  
Ssr Markers ◽  
Genome Sequence ◽  
Populus Tremuloides ◽  
Populus Trichocarpa ◽  
Interspecific Cross ◽  
Populus Tremula ◽  
Central Position ◽  
Populus Alba

A progeny of 130 F1 individuals of an interspecific cross between Populus tremula L. and Populus tremuloides Michx. was used for genetic mapping of linkage group XIX with SSR markers based on the Populus trichocarpa Torr. & A. Gray genome sequence. Several fully sex-linked SSR markers were identified and mapped to a central position on the male P. tremuloides map of linkage group XIX. For the SSR markers tested here, the position on the assembled P. trichocarpa genome sequence is known, allowing sex-linked markers to be assigned to the central region of scaffold/chromosome 19 of P. trichocarpa. The sex linkage of the SSR markers was validated in other P. tremula × P. tremuloides crosses and also tested in Populus alba L. and Populus nigra L.

Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L.

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 544-551 ◽  
Author(s):  
Yonghe Bai ◽  
T. E. Michaels ◽  
K. P. Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Linkage Group ◽  
Bacterial Blight ◽  
Rapd Markers ◽  
Interspecific Cross ◽  
Breeding Population ◽  
Common Bacterial Blight ◽  
Linkage Groups ◽  
Phaseolus Vulgaris L ◽  
Total Contribution

Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.Key words: Phaseolus vulgaris L., common bacterial blight (CBB), polymerase chain reaction (PCR), RAPD markers, linkage groups.

scholarly journals Development of Capsicum EST–SSR markers for species identification and in silico mapping onto the tomato genome sequence

2012 ◽  
Vol 31 (1) ◽  
pp. 101-110 ◽  
Author(s):  
Kenta Shirasawa ◽  
Kohei Ishii ◽  
Cholgwang Kim ◽  
Tomohiro Ban ◽  
Munenori Suzuki ◽  
...  
Keyword(s):  
Species Identification ◽  
Ssr Markers ◽  
Genome Sequence ◽  
In Silico ◽  
Tomato Genome ◽  
In Silico Mapping

scholarly journals Genome sequencing and phylogenetic analysis of allotetraploid Salix matsudana Koidz

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Zhang ◽  
Huwei Yuan ◽  
Yujuan Li ◽  
Yanhong Chen ◽  
Guoyuan Liu ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Tree Species ◽  
Evolutionary History ◽  
Genetic Basis ◽  
Populus Trichocarpa ◽  
Willow Species ◽  
History Of ◽  
Salix Matsudana ◽  
Salix Matsudana Koidz

AbstractPolyploidy is a common phenomenon among willow species. In this study, genome sequencing was conducted for Salix matsudana Koidz (also named Chinese willow), an important greening and arbor tree species, and the genome of this species was compared with those of four other tree species in Salicaceae. The total genome sequence of S. matsudana was 655.72 Mb in size, with repeated sequences accounting for 45.97% of the total length. In total, 531.43 Mb of the genome sequence could be mapped onto 38 chromosomes using the published genetic map as a reference. The genome of S. matsudana could be divided into two groups, the A and B genomes, through homology analysis with the genome of Populus trichocarpa, and the A and B genomes contained 23,985 and 25,107 genes, respectively. 4DTv combined transposon analysis predicted that allotetraploidy in S. matsudana appeared ~4 million years ago. The results from this study will help reveal the evolutionary history of S. matsudana and lay a genetic basis for its breeding.

Transferability, polymorphism and effectiveness for genetic mapping of the Pummelo (Citrus grandis Osbeck) EST-SSR markers

2013 ◽  
Vol 155 ◽  
pp. 85-91 ◽  
Author(s):  
Lijun Chai ◽  
Manosh Kumar Biswas ◽  
Hualin Yi ◽  
Wenwu Guo ◽  
Xiuxin Deng
Keyword(s):  
Genetic Mapping ◽  
Ssr Markers

scholarly journals The Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on 5S rDNA Non-Transcribed Spacer Polymorphism

Forests ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1092 ◽  
Author(s):  
Alexandrov ◽  
Karlov
Keyword(s):  
Molecular Markers ◽  
Tree Species ◽  
5S Rdna ◽  
Populus Tremula ◽  
Plant Science ◽  
Populus Alba ◽  
Natural Hybrid ◽  
Species Specific ◽  
Genetics Of Populations

The Populus L. genus includes tree species that are botanically grouped into several sections. This species successfully hybridizes both in the same section and among other sections. Poplar hybridization widely occurs in nature and in variety breeding. Therefore, the development of poplar species’ specific molecular markers is very important. The effective markers for trees of the Aigeiros Duby section have recently been developed using the polymorphism of 5S rDNA non-transcribed spacers (NTSs). In this article, 5S rDNA NTS-based markers were designed for several species of the Leuce Duby section. The alb9 marker amplifies one fragment with the DNA matrix of P. alba and P. × canescens (natural hybrid P. alba × P. tremula). The alb2 marker works the same way, except for the case with Populus bolleana. In this case, the amplification of three fragments was observed. The tremu1 marker amplification was detected with the DNA matrix of P. tremula and P. × canescens. Thus, the developed markers may be applied as a useful tool for P. alba, P. tremula, P. × canescens, and P. bolleana identification in various areas of plant science such as botany, dendrology, genetics of populations, variety breeding, etc.

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