Ectomycorrhizal fungal communities of nursery-inoculated seedlings outplanted on clear-cut sites in northern Alberta

2006 ◽  
Vol 36 (7) ◽  
pp. 1684-1694 ◽  
Author(s):  
André Gagné ◽  
Jean-Luc Jany ◽  
Jean Bousquet ◽  
Damase P Khasa
Keyword(s):  
Ssr Markers ◽  
Ectomycorrhizal Fungi ◽  
Picea Glauca ◽  
Pinus Contorta ◽  
Morphological Characterization ◽  
Fungal Communities ◽  
Volume Index ◽  
Clear Cut ◽  
And Control ◽  
Northern Alberta

Seedlings from three conifer species (Pinus contorta Doug. ex Loud. var. latifolia Englem., Picea glauca (Moench) Voss, and Picea mariana (Mill.) BSP) were planted on two clear-cut sites in Alberta, Canada, after inoculation in the nursery with strains of six different ectomycorrhizal species (Hebeloma longicaudum, Laccaria bicolor,Paxillus involutus,Pisolithus tinctorius,Rhizopogon vinicolor, and Suillus tomentosus). Five and 6 years after planting, morphological characterization and molecular typing techniques (internal transcribed spacer – restriction fragment length polymorphism (ITS-RFLP) and simple sequence repeat (SSR) markers) were used to identify the ectomycorrhizal fungal communities and to assess the occurrence of the inoculated ectomycorrhizal fungi on host roots. Ectomy corrhi zae recovered from the roots of the planted trees on each of the two sites showed little diversity, with a total of 16 and 19 ITS-RFLP patterns corresponding to 11 and 13 ectomycorrhizal taxa, respectively. The most abundant ectomycorrhizal fungi found on colonized roots were ascomycetes and the widespread basidiomycete Amphinema byssoides. Amongst the six introduced fungal strains, only L. bicolor UAMH 8232 was detected on one site after 5 and 6 years, as determined using six SSR markers. Although not detected after 5 years, some of the introduced strains might have had a positive effect on the early growth of the trees before their replacement by competing species, because significant differences in plot volume index were detected between inoculation and control treatments.

scholarly journals Regeneration of White Spruce Under Aspen Canopies: Seeding, Planting, and Site Preparation

2000 ◽  
Vol 15 (4) ◽  
pp. 177-182 ◽  
Author(s):  
James D. Stewart ◽  
Simon M. Landhäusser ◽  
Kenneth J. Stadt ◽  
Victor J. Lieffers
Keyword(s):  
Populus Tremuloides ◽  
Picea Glauca ◽  
Germination Rate ◽  
White Spruce ◽  
Seed Rain ◽  
Growing Seasons ◽  
Significant Difference ◽  
Different Types ◽  
And Control ◽  
Northern Alberta

Abstract This study investigated the establishment and survival of naturally and artificially seeded white spruce (Picea glauca), as well as three sizes of planted white spruce stock, on different types of scarification beneath mature aspen (Populus tremuloides) in northern Alberta. White spruce seed rain, natural and hand-seeded regeneration establishment and survival, and understory vegetation response were monitored for 4 yr on scarified strips (light blading, heavy blading, and ridged) and on undisturbed controls. Despite a heavy seed rain in 1993, seedling establishment on control plots was almost nil. On scarified plots, the number of seeds required to produce a live seedling after 3 growing seasons ranged from 15 to 37 and up to 68 in one site. Most mortality occurred during the summer. Germination rate on the hand-seeded plots ranged from 19 to 28% on the scarified strips. There was no significant difference in survival from seed (15%) after three growing seasons among the three scarification treatments. Survival of planted stock was 98% on scarified strips and 96% on control strips. Diameter growth was least on control plots but was not significantly different among the scarified treatments. In contrast, height increment was greatest on the light blading treatment and differed little among the other two treatments and control. These results suggest that spruce regeneration in aspen forests can be promoted by scarification or underplanting. West. J. Appl. For. 15(4):177–182.

scholarly journals Ectomycorrhizal Fungi Associated with Pinus densiflora Seedlings under Flooding Stress

2021 ◽  
Vol 13 (8) ◽  
pp. 4367
Author(s):  
Yoonhee Cho ◽  
Shinnam Yoo ◽  
Myung Soo Park ◽  
Ji Seon Kim ◽  
Chang Sun Kim ◽  
...  
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Internal Transcribed Spacer ◽  
Pinus Densiflora ◽  
Red Pine ◽  
Fungal Communities ◽  
Thelephora Terrestris ◽  
Suillus Luteus ◽  
Flooding Stress ◽  
Pine Seedlings ◽  
Rhizopogon Luteolus

Flooding is an environmental stress for plants that not only limits aeration and nutrient acquisition, but also disturbs underground plant-associated fungal communities. Despite frequent flooding, red pine (Pinus densiflora) seedlings thrive in streamside environments. However, whether the compatible ectomycorrhizal fungi (EMF) of red pine are affected by natural flooding is unclear. As EMF are vital symbionts for the development of many trees and allow them to overcome various environmental stresses, in this study, the EMF species associated with red pine seedlings in a streamside environment in Korea were investigated after flooding. The EMF species in 47 seedlings collected from the streamside site were identified by observing their different morphotypes using internal transcribed spacer sequence analysis, and a total of 10 EMF species were identified. The EMF species diversity was lower than that in samples collected from a nearby forest analyzed as a control. The dominant EMF species of streamside seedlings included Amphinema spp., Rhizopogon luteolus, Suillus luteus, and Thelephora terrestris. This study could serve as a basis for investigating the mechanisms by which advantageous EMF aid plant development under flooding stress.

scholarly journals Refining tree size and dose-response functions for control of invasive Pinus contorta

2021 ◽  
pp. 1-36
Author(s):  
Carol A. Rolando ◽  
Brian Richardson ◽  
Thomas S.H. Paul ◽  
Chanatda Somchit
Keyword(s):  
Dose Response ◽  
Pinus Contorta ◽  
Tree Size ◽  
Volume Index ◽  
Mortality Data ◽  
Field Calculation ◽  
Crown Area ◽  
Crown Diameter ◽  
Crown Volume ◽  
Canopy Area

Abstract Exotic conifers are rapidly spreading in many regions of New Zealand, as well as in many other countries, with detrimental impacts on both natural ecosystems and some productive sector environments. Herbicides, in particular the active ingredient (a.i.) triclopyr, are an important tool to manage invasive conifers, yet there is a paucity of information that quantifies the amount of herbicide required to kill trees of different sizes when applied as a basal bark treatment. Two sequential experiments were conducted to define the amount of triclopyr required to kill individual invasive Pinus contorta trees of different sizes when applied in a methylated seed oil to bark (either the whole stem or base of the tree) and to determine which tree size variates (height (HT), diameter at breast height (DBH), crown diameter (CD)), or derived attributes (crown area, crown volume index) best characterised this dose-response relationship. The outcomes of the dose-response research were compared to field operations where triclopyr was applied to the bark of trees from an aerial platform. Applying the herbicide to the whole stem, as opposed to the base of the tree only, significantly increased treatment efficacy. The tree size variates DBH, CD, crown area and crown volume index all provided good fits to the tree mortality data, with >91% prediction accuracy. Of these variates, crown diameter provided the most practical measure of tree size for ease of in-field calculation of dose by an operator. Herbicide rates used in field operations were 7 to 8 times higher than lethal doses calculated from experimental data. Our results highlight the potential for substantial reductions in herbicide rates for exotic conifer control, especially if dose-response data are combined with remotely sensed quantitative measurements of canopy area or volume using new precision technologies such as unmanned aerial vehicles.

scholarly journals Bringing the Virus Back Home: Media Representations of Ukrainian Labour Migrants in a Time of Pandemic

2021 ◽  
pp. 088832542110187
Author(s):  
Alexander Tymczuk
Keyword(s):  
Spatial Organization ◽  
Frame Analysis ◽  
Media Representations ◽  
Potential Danger ◽  
Economic Resource ◽  
Clear Cut ◽  
And Control ◽  
Globalized World ◽  
The Way

In a globalized world where mobility and movement is at its essence, the movement of viruses paradoxically causes a preoccupation with boundaries, containment, and control over borders, and thus keeping the “dangerous” outside separated from the “safe” inside. Through a qualitative thematic and frame analysis of news articles published on 12 Ukrainian news sites, I found that Ukrainian labour migrants conceptually constitute a challenge to such a clear-cut spatial organization in a time of a pandemic. Labour migrants are part of the national “we,” but their presence in the dangerous outside excludes them from the “imagined immunity.” This ambiguity is evident in the way labour migrants were portrayed during the first months of the outbreak in Ukraine. Initially, Ukrainian labour migrants were depicted as a potential danger, and then blamed for bringing the virus back home. However, the framing of the labour migrants as a danger is only part of the story, and the image of a scapegoat was eventually replaced with images of an economic resource and a victim. Thus, Ukrainian labour migrants have been the object of vilification, heroization, as well as empathy during the various phases of the outbreak. I would argue that these shifting frames are connected to the ambiguous conceptualization of Ukrainian labour migrants in general.

Evaluation of planting sites common to a southeast Alaska clear-cut. IV. Nutrient levels in ectomycorrhizal Sitka spruce seedlings

1987 ◽  
Vol 17 (4) ◽  
pp. 340-345 ◽  
Author(s):  
Roy C. Sidle ◽  
Charles G. Shaw III
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Mineral Soil ◽  
Sitka Spruce ◽  
Southeast Alaska ◽  
Clear Cut ◽  
The North ◽  
Rotten Wood ◽  
North Side ◽  
Foliar Concentrations ◽  
Nutritional Benefits

Sitka spruce (Piceasitchensis (Bong.) Carr.) seedlings colonized by the ectomycorrhizal fungi Laccarialaccata (Scop. ex Fr.) Berk and Br., Hebelomacrustuliniforme (Bull, ex St. Amans) Quel., or Cenococcumgeophilum Fr. and a noninoculated control were planted on rotten wood, undisturbed duff, undisturbed duff on the north side of large stumps, and exposed mineral soil in an area recently clear-cut on Chichagof Island, southeast Alaska. Uptake and concentrations of N, P, K, Ca, Mg, and several micronutrients in foliage, stems, and roots were determined 2 years after outplanting. There were no significant interactions between fungal treatments and microsite types for uptake or concentration of any nutrient. Uptake and concentrations of N and P in foliage and roots did not differ significantly among fungal treatments. Foliar K and Cu concentrations were significantly higher in control seedlings than in those with L. laccata ectomycorrhizae. Calcium was the only nutrient analyzed that was present in higher concentrations in seedlings inoculated with H. crustuliniforme than in controls. Concentrations of foliar P, Mn, and Zn were significantly greater in seedlings grown on undisturbed duff (protected north side) than in seedlings grown on exposed mineral soil. However, foliar concentrations of all nutrients tested were not significantly different between seedlings grown on undisturbed duff (unprotected sites) and those grown on mineral soil. Seedlings grown on rotten wood had significantly greater concentrations of foliar Ca than seedlings grown on either exposed mineral soil or undisturbed duff. The on-site colonization of control seedlings after outplanting indicates that resident ectomycorrhizal fungi readily establish and appear to provide equal if not improved nutrient benefits compared to inoculated seedlings. Greater nutritional benefits were derived by planting on certain microsite types (duff and protected duff) than by inoculating with specific ectomycorrhizal fungi.

Characterization of the bacterial flora associated with root systems of Pinus contorta var. latifolia

1978 ◽  
Vol 24 (12) ◽  
pp. 1520-1525 ◽  
Author(s):  
J. A. Dangerfield ◽  
D. W. S. Westlake ◽  
F. D. Cook
Keyword(s):  
Amino Acid ◽  
Pinus Contorta ◽  
Bacterial Flora ◽  
Root Systems ◽  
Growth Media ◽  
Agricultural Crops ◽  
Control Soil ◽  
Mature Tree ◽  
And Control

Root systems of young and mature lodgepole pine (Pinus contorta Dougl. var. latifolia Englem.) were removed from forest stands and the associated aerobic bacterial flora isolated. Characterization of rhizoplane and control soil isolates from these tree root systems demonstrated differences from that reported for agricultural crops. Ammonifying, proteolytic, and amylolytic organisms were proportionately reduced within the rhizoplane. The rhizoplane organisms grew more slowly than the control soil isolates, although they responded in greater numbers to the addition of an amino acid supplement to the growth media. The rhizoplane organisms also showed an increased ability to solubilize phosphate. The chitinolytic organisms were suppressed within the rhizoplane of the mature tree but were stimulated by the young trees. With this exception, the rhizoplane microflora of older and younger trees were similar.

An Historical Perspective of the Giriama and Witchcraft Control

Africa ◽  
1979 ◽  
Vol 49 (2) ◽  
pp. 112-133 ◽  
Author(s):  
Cynthia Brantley
Keyword(s):  
Historical Perspective ◽  
African Society ◽  
Clear Cut ◽  
Opening Paragraph ◽  
Protection And Control ◽  
And Control

Opening ParagraphWherever belief in witchcraft permeated an African society, fear prevailed and people demanded protection and control. Even though the degree of African concern about witchcraft was not always appreciated by outsiders, it was possible, at least in centralized societies, for such outsiders to discern the processes that were involved in its control. A king or a priest who failed to control the spread of witchcraft and to alleviate the fear was unlikely to maintain his authority for long. In non-centralized societies, the problem of witchcraft and the means of control were less clear-cut. Solutions were rarely obvious and easy.

scholarly journals Auswirkungen restriktiver Ernährung auf die Wachstumsleistung von Ziegenlämmern

1999 ◽  
Vol 42 (3) ◽  
pp. 281-294
Author(s):  
Y. Toukourou ◽  
K.-J. Peters
Keyword(s):  
Growth Performance ◽  
Energy Demand ◽  
Daily Weight Gain ◽  
Feed Restriction ◽  
Control Group ◽  
Control Groups ◽  
Experimental Animals ◽  
Clear Cut ◽  
And Control ◽  
Different Levels

Abstract. Title of the paper: Impaet of feed restriction on the growth performance of goat kids The influence of differential feeding levels on growth performance in 72 goat kids "Bunte Deutsche Edelziege" during the pre-weaning period was examined. The 72 animals were assigned to a control group and two experimental groups that received respectively 20% and 40% less milk/less concentrate compared to the control (fed at 2.4 times energy demand for maintenance). The experimental gained animals significantly less relative to the control group. However, during the subsequent realimentation period when all animals were fed at a energy level of 2.4 times maintenance same treatment, the daily weight gain among the kids was in inverse proportion to the level ofmilk deprivation in the pre-weaning phase. The rapid growth among the experimental animals was such that the initial differences in body weight between the experimental and control groups were fully compensated. Growth performance of kids with respect to different levels of concentrated feed was less clear cut and d.ffered significantly only behveen the group that received the lowest feed level relative to all the other groups.

scholarly journals Planted stock performance 10 years after partial cutting in west-central British Columbia

2010 ◽  
Vol 86 (1) ◽  
pp. 118-129 ◽  
Author(s):  
M J Waterhouse ◽  
E. C. Wallich ◽  
N. M. Daintith ◽  
H. M. Armleder
Keyword(s):  
Picea Glauca ◽  
Group Selection ◽  
Pinus Contorta ◽  
Rangifer Tarandus ◽  
Lodgepole Pine ◽  
Light Level ◽  
Frost Damage ◽  
Partial Cutting ◽  
Partial Harvest ◽  
Interior Spruce

Mature lodgepole pine (Pinus contorta) forests were harvested using group selection (GS) (0.02-ha openings) and irregular group shelterwood (IGS) (0.05-ha openings) systems to maintain arboreal and terrestrial lichens in the winter range of northern woodland caribou (Rangifer tarandus caribou). Ten years after planting, lodgepole pine showed excellent survival, but were smaller in the partial cut openings compared to the clearcuts. Pine grew less in the Sub-Boreal Pine–Spruce biogeoclimatic subzone (SBPSxc) than in the Montane Spruce subzone (MSxv), and trees were smaller in the GS versus IGS treatment within the MSxv subzone. Interior spruce (Picea glauca × engelmannii) grew best in the MSxv and partial cut treatments, but was significantly affected by summer frost in the clearcuts. In an operational-scale Adaptive Management trial, openings were enlarged to 0.15 ha, and both pine and spruce showed excellent survival, minimal frost damage, and 10-year size similar to clearcut conditions. This study suggests that lodgepole pine and interior spruce can be successfully regenerated in partial cut openings with acceptable growth in gaps of 0.15 ha. Key words: caribou, group selection, interior spruce, irregular group shelterwood, light level, lodgepole pine, Montane Spruce zone, partial harvest, soil moisture, soil temperature, Sub-Boreal Pine Spruce zone, summer frost

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