Assessment of genetic diversity in Tectona grandis using amplified fragment length polymorphism markers

2005 ◽  
Vol 35 (4) ◽  
pp. 1017-1022 ◽  
Author(s):  
Madan K Shrestha ◽  
Hugo Volkaert ◽  
Dominique Van Der Straeten
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Variance ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Tectona Grandis ◽  
Principal Coordinate Analysis ◽  
Indian Populations ◽  
The Past ◽  
Geographical Regions

There is much concern over teak (Tectona grandis L. f.) genetic resources because of uncontrolled logging during the past centuries. We investigated patterns of genetic variation within and among nine populations of T. grandis from diverse geographical regions in India, Thailand, and Indonesia using amplified fragment length polymorphism (AFLP). An analysis of molecular variance (AMOVA) showed that 57% of total genetic variance occurred within populations, the remaining 43% occurred between populations. Cluster analysis and principal coordinate analysis indicated that Indian populations are clearly separated from those in Thailand and Indonesia. However, the Berbera population from Orissa near the Indian northeastern coast is an exception, as it seems to have associations with both the Indian and the Thai–Indonesian populations. The Thai and Indian populations cannot mutually be separated in this investigation. The comparisons between Indian populations suggest that the northwestern Allapally plains population (Maharastra) is distinct from the two southern Indian populations that were analyzed.

Assessment of genetic diversity of Guizotia abyssinica (L.f.) Cass. (Asteraceae) from Ethiopia using amplified fragment length polymorphism

2008 ◽  
Vol 6 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Mulatu Geleta ◽  
Tomas Bryngelsson ◽  
Endashaw Bekele ◽  
Kifle Dagne
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Distance ◽  
Amplified Fragment Length Polymorphism ◽  
Principal Coordinate Analysis ◽  
Gene Bank ◽  
Guizotia Abyssinica ◽  
Level Analysis

Seventeen populations of niger (Guizotia abyssinica), representing all regions in Ethiopia where this crop is grown, were investigated using the amplified fragment length polymorphism (AFLP) technique, in order to determine the extent and distribution of its genetic diversity. A total of 539 AFLP loci were scored using seven primer combinations applied to 170 individual plants. Of these, 90% were polymorphic and all the individuals investigated were genetically unique. Despite the fact that most of the variation was within populations, populations were differentiated at a significant level (analysis of molecular variance; P < 0.001). There were no significant differences between populations in relation to the extent and altitude of cultivation. A significant positive correlation was revealed between Nei's standard genetic distance and geographic distance. Cluster analysis and principal coordinate analysis revealed that populations from the same regions were clustered together in most cases. Further collection of niger germplasm from areas underrepresented in gene bank collections is recommended.

Structure of worldwide populations of Lasioderma serricorne (Coleoptera: Anobiidae) as revealed by amplified fragment length polymorphism profiles

2006 ◽  
Vol 96 (2) ◽  
pp. 111-116 ◽  
Author(s):  
M.P. Blanc ◽  
N. Lugon-Moulin ◽  
C. Panighini ◽  
H. Pijnenburg ◽  
L. Rossi
Keyword(s):  
Population Study ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Origin ◽  
Amplified Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Lasioderma Serricorne ◽  
Insect Dispersal ◽  
Geographical Regions

AbstractThe cigarette beetle Lasioderma serricorne through transportation affects the infestation of stored tobacco. Using amplified fragment length polymorphism (AFLP), DNA polymorphisms were assessed in 16 populations of L. serricorne collected from 15 countries. The dendrograms constructed from profile distance matrices revealed well-supported colony clusters. There was no clear clustering as a function of the geographic origin of the samples. The results suggest extensive insect dispersal among geographical regions due to movement of infested commodities worldwide. This first AFLP population study of a stored-product insect demonstrates the potential of AFLP for distinguishing L. serricorne populations.

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