Structure of worldwide populations of Lasioderma serricorne (Coleoptera: Anobiidae) as revealed by amplified fragment length polymorphism profiles

2006 ◽  
Vol 96 (2) ◽  
pp. 111-116 ◽  
Author(s):  
M.P. Blanc ◽  
N. Lugon-Moulin ◽  
C. Panighini ◽  
H. Pijnenburg ◽  
L. Rossi
Keyword(s):  
Population Study ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Origin ◽  
Amplified Fragment Length Polymorphism ◽  
Dna Polymorphisms ◽  
Lasioderma Serricorne ◽  
Insect Dispersal ◽  
Geographical Regions

AbstractThe cigarette beetle Lasioderma serricorne through transportation affects the infestation of stored tobacco. Using amplified fragment length polymorphism (AFLP), DNA polymorphisms were assessed in 16 populations of L. serricorne collected from 15 countries. The dendrograms constructed from profile distance matrices revealed well-supported colony clusters. There was no clear clustering as a function of the geographic origin of the samples. The results suggest extensive insect dispersal among geographical regions due to movement of infested commodities worldwide. This first AFLP population study of a stored-product insect demonstrates the potential of AFLP for distinguishing L. serricorne populations.

scholarly journals High-Resolution Genotyping of Streptococcus pyogenesSerotype M1 Isolates by Fluorescent Amplified-Fragment Length Polymorphism Analysis

1999 ◽  
Vol 37 (6) ◽  
pp. 1948-1952 ◽  
Author(s):  
Meeta Desai ◽  
Androulla Efstratiou ◽  
Robert George ◽  
John Stanley
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Geographic Origin ◽  
Amplified Fragment Length Polymorphism ◽  
Discriminatory Power ◽  
Polymorphism Analysis ◽  
Worldwide Distribution ◽  
High Discriminatory Power ◽  
Typing Methods

We have used fluorescent amplified-fragment length polymorphism (FAFLP) analysis to subtype clinical isolates of Streptococcus pyogenes serotype M1. Established typing methods define most M1 isolates as members of a clone that has a worldwide distribution and that is strongly associated with invasive diseases. FAFLP analysis simultaneously sampled 90 to 120 loci throughout the M1 genome. Its discriminatory power, precision, and reproducibility were compared with those of other molecular typing methods. Irrespective of disease symptomatology or geographic origin, the majority of the clinical M1 isolates shared a single ribotype, pulsed-field gel electrophoresis macrorestriction profile, and emm1 gene sequence. Nonetheless, among these isolates, FAFLP analysis could differentiate 17 distinct profiles, including seven multi-isolate groups. The FAFLP profiles of M1 isolates reproducibly exhibited between 1 and more than 20 amplified fragment differences. The high discriminatory power of genotyping by FAFLP analysis revealed genetic microheterogeneity and differentiated otherwise “identical” M1 isolates as members of a clone complex.

Assessment of genetic diversity in Tectona grandis using amplified fragment length polymorphism markers

2005 ◽  
Vol 35 (4) ◽  
pp. 1017-1022 ◽  
Author(s):  
Madan K Shrestha ◽  
Hugo Volkaert ◽  
Dominique Van Der Straeten
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Genetic Variance ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism ◽  
Tectona Grandis ◽  
Principal Coordinate Analysis ◽  
Indian Populations ◽  
The Past ◽  
Geographical Regions

There is much concern over teak (Tectona grandis L. f.) genetic resources because of uncontrolled logging during the past centuries. We investigated patterns of genetic variation within and among nine populations of T. grandis from diverse geographical regions in India, Thailand, and Indonesia using amplified fragment length polymorphism (AFLP). An analysis of molecular variance (AMOVA) showed that 57% of total genetic variance occurred within populations, the remaining 43% occurred between populations. Cluster analysis and principal coordinate analysis indicated that Indian populations are clearly separated from those in Thailand and Indonesia. However, the Berbera population from Orissa near the Indian northeastern coast is an exception, as it seems to have associations with both the Indian and the Thai–Indonesian populations. The Thai and Indian populations cannot mutually be separated in this investigation. The comparisons between Indian populations suggest that the northwestern Allapally plains population (Maharastra) is distinct from the two southern Indian populations that were analyzed.

scholarly journals Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)

2005 ◽  
Vol 28 (2) ◽  
pp. 267-270 ◽  
Author(s):  
Michelle Mantovani Gonçalves ◽  
Manoel Victor Franco Lemos ◽  
Pedro Manoel Galetti Junior ◽  
Patrícia Domingues de Freitas ◽  
Manuel Antonio Andrade Furtado Neto
Keyword(s):  
Genetic Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Litopenaeus Vannamei ◽  
Fragment Length Polymorphism ◽  
Amplified Fragment Length Polymorphism
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