Site temperatures influence seasonal changes in terpene composition in Douglas-fir vegetative buds and current-year foliage
Over a 3-year period (19982000), variations in terpene composition was measured in vegetative buds of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) from six sites varying in elevation and geographic location with sampling from early spring to midsummer. Eleven terpenes were found in virtually all samples (tricyclene, α-pinene, camphene, sabinene, β-pinene, myrcene, Δ-3-carene, limonene, β-phellandrene, terpinolene, and bornyl acetate) and represented an average of 87% of the total terpenes. In each year, composition of the terpene mix varied significantly (P [Formula: see text] 0.05) for all sites and dates, with some significant site and date interactions. Degree-day accumulations were calculated for all sites and years. Patterns of change in terpene composition between sites, areas, and years were strongly related to the temperature regimes associated with site and year.