Polyamines conjuguées et différenciation florale chez le sapin de Douglas (Pseudotsuga menziesii (Mirb.) Franco)

1998 ◽  
Vol 76 (5) ◽  
pp. 782-790
Author(s):  
EL-Hassania Daoudi ◽  
Marc Bonnet-Masimbert

To characterize as early as possible the status of flowering differentiation in Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), the variation of bound or conjugated polyamines is studied in the shoots of young plants (6 years old) during the period covering flowering initiation (between 30 and 75 days after bud burst). These compounds are also quantified in sexual and vegetative buds. The quantitative effects of treatments susceptible to stimulate the flowering (application of gibberellins and (or) calcium nitrate) on the level of conjugated polyamines are investigated in the shoots. The combination of gibberellins to calcium nitrate application strongly increases the flowering. Conjugated polyamines are particularly abundant in sexual buds and in the shoots of flowering plants. Sexual buds are also characterized by a ratio of conjugated putrescine to conjugated spermidine about four to six times higher than in vegetative buds. The accumulation of conjugated polyamines in the shoots accompanies bud sexualization, which suggests that conjugated polyamines may constitute potential markers of floral initiation in Douglas-fir.Key words: floral induction, flowering marker, conjugated polyamines, Douglas-fir.

1994 ◽  
Vol 24 (9) ◽  
pp. 1854-1863 ◽  
Author(s):  
E.H. Daoudi ◽  
P. Doumas ◽  
M. Bonnet-Masimbert

To characterize as early as possible the status of flowering differentiation in Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco), the variation of amino acids and polyamines was studied in the shoots during the period covering flowering initiation (between 4 and 10 weeks after bud burst). These compounds were also quantified in sexual and vegetative buds. The qualitative and quantitative effects of treatments susceptible to stimulate flowering (application of gibberellins (GA) and (or) calcium nitrate (N)) were observed in the shoots. Only N and N + GA treated trees flowered, the flowering being especially abundant for trees treated by N + GA. Arginine and proline were generally more abundant in sexual buds than in vegetative ones and in the shoots of flowering plants than in the control shoots. Sexual buds were characterized by a ratio of putrescine/(spermidine + spermine) about two times lower than in vegetative buds, mainly owing to a lower level in putrescine. The accumulation of polyamines in the shoots accompanied bud sexualization, which suggests that polyamines may constitute potential markers, probably rather early ones (4–6 weeks after bud burst), of floral initiation in Douglas-fir. This possibility is discussed.


2005 ◽  
Vol 54 (1-6) ◽  
pp. 218-227 ◽  
Author(s):  
J. K. Hansen ◽  
H. Wellendorf ◽  
E. D. Kjær

Abstract An evaluation of a seedling seed orchard in Denmark of Coastal Douglas-fir showed variation between open pollinated families in their susceptibility to windthrow, height and diameter growth, stem straightness, number of forks at age 10 and bud burst at age 9. Individual narrow-sense heritability was 0.63 for bud burst, 0.40 for height and diameter, respectively, 0.48 for stem straightness, 0.20 for number of forks and 0.17 for windthrow susceptibility. Fast growing families were characterised by late bud burst, and poorer stem straightness. The approach of using seedling seed orchards in Denmark is discussed in relation to genetic gains, genetic diversity and risk of inbreeding. Predicted gains concerning a reduction of windthrow susceptibility were poor due to low genetic variability. In multi-trait selection scenarios aiming to improve growth, stem straightness and reducing windthrow susceptibility, the approach of using seedling seed orchards demonstrated that it might be possible to obtain gains in height above 5% combined with significant improvements of stem straightness while retaining an effective population size above 50.


2003 ◽  
Vol 33 (11) ◽  
pp. 2269-2273 ◽  
Author(s):  
Jason R Nault

Over a 3-year period (1998–2000), variations in terpene composition was measured in vegetative buds of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) from six sites varying in elevation and geographic location with sampling from early spring to midsummer. Eleven terpenes were found in virtually all samples (tricyclene, α-pinene, camphene, sabinene, β-pinene, myrcene, Δ-3-carene, limonene, β-phellandrene, terpinolene, and bornyl acetate) and represented an average of 87% of the total terpenes. In each year, composition of the terpene mix varied significantly (P [Formula: see text] 0.05) for all sites and dates, with some significant site and date interactions. Degree-day accumulations were calculated for all sites and years. Patterns of change in terpene composition between sites, areas, and years were strongly related to the temperature regimes associated with site and year.


Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 744-748 ◽  
Author(s):  
Jerry E. Weiland ◽  
Bryan R. Beck ◽  
Anne Davis

Pythium species are common soilborne oomycetes that occur in forest nursery soils throughout the United States. Numerous species have been described from nursery soils. However, with the exception of P. aphanidermatum, P. irregulare, P. sylvaticum, and P. ultimum, little is known about the potential for other Pythium species found in nursery soils to cause damping-off of tree seedlings. A greenhouse study was conducted to evaluate the pathogenicity and virulence of 44 Pythium isolates representing 16 species that were originally recovered from soil at three forest nurseries in Washington and Oregon. Seeds of Douglas-fir (Pseudotsuga menziesii) were planted into soil infested with each of the isolates. Seedling survival, the number of surviving seedlings with necrotic root lesions, and taproot length were evaluated 4 weeks later. Responses of Douglas-fir to inoculation varied significantly depending on Pythium species and isolate. Eight species (P. dissotocum, P. irregulare, P. aff. macrosporum, P. mamillatum, P. aff. oopapillum, P. rostratifingens, P. sylvaticum, and P. ultimum var. ultimum) significantly reduced the number of surviving seedlings compared to the noninoculated treatment. However, all Pythium species caused a greater percentage of seedlings to develop root lesions (total mean 40%) than was observed from noninoculated seedlings (17%). Taproot length varied little among Pythium treatments and was not a useful character for evaluating pathogenicity. Results confirm the ability of P. irregulare, P. mamillatum, and P. ultimum var. ultimum to cause damping-off of Douglas-fir seedlings, and are indicative that other species such as P. dissotocum, P. aff. macrosporum, P. aff. oopapillum, P. rostratifingens, and P. sylvaticum may also be responsible for seedling loss.


1998 ◽  
Vol 28 (8) ◽  
pp. 1198-1206 ◽  
Author(s):  
Paige E Axelrood ◽  
William K Chapman ◽  
Keith A Seifert ◽  
David B Trotter ◽  
Gwen Shrimpton

Poor performance of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) plantations established in 1987 has occurred in southwestern British Columbia. Affected sites were planted with 1-year-old container stock that exhibited some root dieback in the nursery. A study was initiated in 1991 to assess Cylindrocarpon and Fusarium root infection in planted and naturally regenerating (natural) Douglas-fir seedlings from seven affected plantations. Percentages of seedlings harboring Cylindrocarpon spp.and percent root colonization were significantly greater for planted seedlings compared with natural seedlings. A significant linear trend in Cylindrocarpon root colonization was observed for planted seedlings with colonization levels being highest for roots closest to the remnants of the root plug and decreasing at distances greater than 10cm from that region. This trend in Cylindrocarpon colonization was not observed for natural seedlings. Cylindrocarpon destructans (Zins.) Scholten var. destructans and C.cylindroides Wollenw. var. cylindroides were the only species isolated from planted and natural conifer seedlings. For most sites, percentage of seedlings harboring Fusarium spp.and percent Fusarium root colonization were less than for Cylindrocarpon. Recovery of Fusarium spp.from seedlings and root colonization levels were not significantly different for planted and natural seedlings from all sites.


Author(s):  

Abstract A new distribution map is provided for Contarinia pseudotsugae Condrashoff (Diptera: Cecidomyiidae). Hosts: Douglas fir (Pseudotsuga menziesii). Information is given on the geographical distribution in Europe (Belgium, France, Germany and Netherlands) and North America (Canada, British Columbia, USA, California, Idaho, Michigan, Montana, Oregon, Pennsylvania and Washington).


Author(s):  

Abstract A new distribution map is provided for Phytophthora pseudotsugae Hamm & Hansen. Host: douglas fir (Pseudotsuga menziesii). Information is given on the geographical distribution in NORTH AMERICA, USA, OR, WA.


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