Tween 80 enhanced TNT mineralization by Phanerochaete chrysosporium

2000 ◽  
Vol 46 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Jonathan Hodgson ◽  
Denis Rho ◽  
Serge R Guiot ◽  
Guy Ampleman ◽  
Sonia Thiboutot ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Culture Medium ◽  
Consumption Rate ◽  
Oxygen Uptake Rate ◽  
Tween 80 ◽  
Control Culture ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Culture ◽  
Activity Levels

The effect of a nonionic surfactant (Tween 80) on 2,4,6-trinitrotoluene (TNT) mineralization by the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767, was investigated in a liquid culture at 20, 50, and 100 mg TNT·L-1. The presence of 1% (w/v) Tween 80, at 20 mg·L-1 TNT, added to a 4-d-old culture, allowed the highest TNT mineralization level, that is 29.3% after 24 d, which is two times more than the control culture, without Tween 80 (13.9%). The mineralization of TNT resumed upon additional Tween 80 supplementation, consequently, 39.0% of the TNT was respired on day 68. Orbital agitation of the fungal culture was found detrimental to TNT mineralization, with or without Tween 80 in the culture medium. The surfactant also stimulated the growth of P. chrysosporium without any notable effect on either the glycerol consumption rate or the extracellular LiP and MnP activity levels. Respirometric assays highlighted some differences between the oxygen uptake rate of the fungal culture supplemented with or without Tween 80. Key words: 2,4,6-trinitrotoluene, TNT, surfactant, white-rot fungus, Phanerochaete chrysosporium, lignin peroxidase, manganese peroxidase.

scholarly journals Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium

2008 ◽  
Vol 74 (17) ◽  
pp. 5359-5365 ◽  
Author(s):  
Avi Matityahu ◽  
Yitzhak Hadar ◽  
Carlos G. Dosoretz ◽  
Paula A. Belinky
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Phanerochaete Chrysosporium ◽  
Gene Families ◽  
White Rot ◽  
Transcriptional Unit ◽  
White Rot Fungus ◽  
Activity Levels ◽  
Protein Activity ◽  
Rnai Technique

ABSTRACT The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.

The Effect of Heavy Metal-Induced Oxidative Stress on the Enzymes in White Rot Fungus Phanerochaete chrysosporium

2014 ◽  
Vol 175 (3) ◽  
pp. 1281-1293 ◽  
Author(s):  
Qihua Zhang ◽  
Guangming Zeng ◽  
Guiqiu Chen ◽  
Min Yan ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

Toxicity of carbon nanotubes to white rot fungus Phanerochaete chrysosporium

2018 ◽  
Vol 162 ◽  
pp. 225-234 ◽  
Author(s):  
Zhu Ming ◽  
Shicheng Feng ◽  
Ailimire Yilihamu ◽  
Shengnan Yang ◽  
Qiang Ma ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Biodegradation of sago effluent by white-rot fungus Phanerochaete chrysosporium

2021 ◽  
Vol 11 (2) ◽  
pp. 91-99
Author(s):  
Vivekanandhan K. ◽  
Dr.M.P. Ayyappadas ◽  
Dr. S. K. Ayyappadas ◽  
R. Renugadevi, ◽  
M. Flory Shobana ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Pyrene and Chrysene Tolerance and Biodegradation Capability of Pleurotus Sajor-Caju

2018 ◽  
Vol 12 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Giuliano Saiu ◽  
Stefania Tronci ◽  
Massimiliano Grosso ◽  
Enzo Cadoni ◽  
Nicoletta Curreli
Keyword(s):  
Liquid Medium ◽  
Copper Sulphate ◽  
Negative Impact ◽  
Fungal Growth ◽  
Tween 80 ◽  
White Rot ◽  
White Rot Fungus ◽  
Polycyclic Aromatic ◽  
Pleurotus Sajor Caju

Introduction: The present work focused on the biodegradation capability of a white-rot fungus, the Pleurotus sajor-caju, when exposed to polycyclic aromatic hydrocarbons. Methods: The research was carried out by using in vitro systems developed on Petri dishes, to evaluate the fungal tolerance to pyrene and chrysene, followed by experiments in liquid medium. The first experimental campaign was necessary to evaluate the conditions promoting fungal growth and tolerance (presence of surfactants, peptone, copper sulphate and lecithin) and it was designed and analysed using statistical techniques. Results: It was found that the fungal population growth is strongly inhibited by chrysene presence. On the other hand, pyrene had a mild negative impact on the mycelia growth, which seemed to be positively influenced by the presence of Tween 80 and copper sulphate. Starting from these results, the behaviour of Pleurotus sajor-caju in presence of pyrene was investigated in liquid medium. Results showed that the depletion of pyrene was evident during a period of 20 days, and removal efficiency was greater than 90%.

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