scholarly journals Gene Silencing by RNA Interference in the White Rot Fungus Phanerochaete chrysosporium

2008 ◽  
Vol 74 (17) ◽  
pp. 5359-5365 ◽  
Author(s):  
Avi Matityahu ◽  
Yitzhak Hadar ◽  
Carlos G. Dosoretz ◽  
Paula A. Belinky
Keyword(s):  
Rna Interference ◽  
Gene Silencing ◽  
Phanerochaete Chrysosporium ◽  
Gene Families ◽  
White Rot ◽  
Transcriptional Unit ◽  
White Rot Fungus ◽  
Activity Levels ◽  
Protein Activity ◽  
Rnai Technique

ABSTRACT The effectiveness of RNA interference (RNAi) is demonstrated in the lignin-degrading fungus Phanerochaete chrysosporium. The manganese-containing superoxide dismutase gene (MnSOD1) was used as the target for RNAi. The plasmid constructed for gene silencing contained a transcriptional unit for hairpin RNA expression. Significantly lower MnSOD expression at both the mRNA and protein activity levels was detected in RNAi transformants. Furthermore, even though P. chrysosporium possesses three copies of the MnSOD gene, this RNAi construct was sufficient to decrease the enzymatic activity by as much as 70% relative to control levels. Implementation of the RNAi technique in P. chrysosporium provides an alternative genetic tool for studies of gene function, particularly of essential genes or gene families.

Tween 80 enhanced TNT mineralization by Phanerochaete chrysosporium

2000 ◽  
Vol 46 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Jonathan Hodgson ◽  
Denis Rho ◽  
Serge R Guiot ◽  
Guy Ampleman ◽  
Sonia Thiboutot ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
Culture Medium ◽  
Consumption Rate ◽  
Oxygen Uptake Rate ◽  
Tween 80 ◽  
Control Culture ◽  
White Rot ◽  
White Rot Fungus ◽  
Fungal Culture ◽  
Activity Levels

The effect of a nonionic surfactant (Tween 80) on 2,4,6-trinitrotoluene (TNT) mineralization by the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767, was investigated in a liquid culture at 20, 50, and 100 mg TNT·L-1. The presence of 1% (w/v) Tween 80, at 20 mg·L-1 TNT, added to a 4-d-old culture, allowed the highest TNT mineralization level, that is 29.3% after 24 d, which is two times more than the control culture, without Tween 80 (13.9%). The mineralization of TNT resumed upon additional Tween 80 supplementation, consequently, 39.0% of the TNT was respired on day 68. Orbital agitation of the fungal culture was found detrimental to TNT mineralization, with or without Tween 80 in the culture medium. The surfactant also stimulated the growth of P. chrysosporium without any notable effect on either the glycerol consumption rate or the extracellular LiP and MnP activity levels. Respirometric assays highlighted some differences between the oxygen uptake rate of the fungal culture supplemented with or without Tween 80. Key words: 2,4,6-trinitrotoluene, TNT, surfactant, white-rot fungus, Phanerochaete chrysosporium, lignin peroxidase, manganese peroxidase.

The Effect of Heavy Metal-Induced Oxidative Stress on the Enzymes in White Rot Fungus Phanerochaete chrysosporium

2014 ◽  
Vol 175 (3) ◽  
pp. 1281-1293 ◽  
Author(s):  
Qihua Zhang ◽  
Guangming Zeng ◽  
Guiqiu Chen ◽  
Min Yan ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

Toxicity of carbon nanotubes to white rot fungus Phanerochaete chrysosporium

2018 ◽  
Vol 162 ◽  
pp. 225-234 ◽  
Author(s):  
Zhu Ming ◽  
Shicheng Feng ◽  
Ailimire Yilihamu ◽  
Shengnan Yang ◽  
Qiang Ma ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Biodegradation of sago effluent by white-rot fungus Phanerochaete chrysosporium

2021 ◽  
Vol 11 (2) ◽  
pp. 91-99
Author(s):  
Vivekanandhan K. ◽  
Dr.M.P. Ayyappadas ◽  
Dr. S. K. Ayyappadas ◽  
R. Renugadevi, ◽  
M. Flory Shobana ◽  
...  
Keyword(s):  
Phanerochaete Chrysosporium ◽  
White Rot ◽  
White Rot Fungus

scholarly journals Biotransformation of Pristine and Oxidized Carbon Nanotubes by the White Rot Fungus Phanerochaete chrysosporium

Nanomaterials ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 1340 ◽  
Author(s):  
Qiang Ma ◽  
Ailimire Yilihamu ◽  
Zhu Ming ◽  
Shengnan Yang ◽  
Mengyao Shi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Multiwalled Carbon Nanotubes ◽  
Phanerochaete Chrysosporium ◽  
Environmental Remediation ◽  
Carbon Nanomaterials ◽  
Carbon Skeleton ◽  
White Rot ◽  
White Rot Fungus ◽  
Multiwalled Carbon ◽  
Complete Degradation

Carbon nanomaterials are widely studied and applied nowadays, with annual production increasing. After entering the environment, the complete degradation of these carbon nanomaterials by microorganisms is proposed as an effective approach for detoxification and remediation. In this study, we evaluated the degradation of pristine multiwalled carbon nanotubes (p-MWCNTs) and oxidized multiwalled carbon nanotubes (o-MWCNTs) by the white rot fungus Phanerochaete chrysosporium, which is a powerful decomposer in the carbon cycle and environmental remediation. Both p-MWCNTs and o-MWCNTs were partially oxidized by P. chrysosporium as indicated by the addition of oxygen atoms to the carbon skeleton in the forms of C=O and O–H bonds. The fungal oxidation led to the shortening of MWCNTs, where precipitated o-MWCNTs showed more short tubes. During the transformation, the defects on the tubes became detached from the carbon skeleton, resulting in decreases of the ID/IG (intensity of D-band/ intensity of G-band) values in Raman spectra. The transformation mechanism was attributed to the enzymatic degradation by laccase and manganese peroxidase excreted by P. chrysosporium. The results collectively indicated that MWCNTs could be transformed by P. chrysosporium, but complete degradation could not be achieved in a short time period. The implications on the environmental risks of carbon nanomaterials are discussed.

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