Impact of an urban effluent on the bacterial community structure in the Arga River (Spain), with special reference to culturable Gram-negative rods

1999 ◽  
Vol 45 (10) ◽  
pp. 826-832 ◽  
Author(s):  
Marisol Goñi-Urriza ◽  
Michèle Capdepuy ◽  
Nathalie Raymond ◽  
Claudine Quentin ◽  
Pierre Caumette
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Sampling Station ◽  
Gram Negative ◽  
Structure Changes ◽  
Sampling Zone ◽  
The Impact ◽  
Urban Effluent

The Arga River is an interesting system in which to study the impact of urban effluent pollution because it receives a single effluent in the form of wastewater discharge from the city of Pamplona. To analyze the extent of this discharge, total bacteria, culturable heterotrophic bacteria, and Gram-negative heterotrophic bacteria were enumerated and 409 isolates of the latter were identified. One sampling station was located upstream from the inflow, while five were located up to 30 km downstream. Bacterial counts increased drastically downstream from the wastewater inflow. Total bacterial numbers decreased along the 30 km downstream, the last station attaining similar values to those recorded upstream from the discharge. However, culturable heterotrophic and Gram-negative heterotrophic bacteria levels generally remained significantly higher within the 30 km zone investigated. Among the 409 isolates identified, Aeromonas spp. were the most frequent both upstream and downstream from the discharge. In contrast, although strains belonging to different genera of Enterobacteriaceae were found in all stations, their occurrence was significantly higher just downstream from the polluted discharge. Acinetobacter spp., which were never found upstream, were detected in all stations below the discharge. Our results clearly show that the bacterial community structure changes definitively downstream from the discharge and that Aeromonas were common throughout the sampling zone. Thus they cannot be considered good indicators of pollution in this setting compared to some genera of Enterobacteriaceae or some species ofAcinetobacter, the distribution of which correlated better with the distance from the wastewater discharge.Key words: Aeromonas, Enterobacteriaceae, sewage, freshwater.

Tu1716 Evaluation of the Impact of Different Commercially Available DNA Extraction Kits and Laboratories for Assessing Bacterial Community Structure in Fecal Samples - Implications for IBD Research

2013 ◽  
Vol 144 (5) ◽  
pp. S-829
Author(s):  
Nicholas A. Kennedy ◽  
Alan Walker ◽  
UK IBD Microbiota Consortia ◽  
UK IBD Genetics Consortia ◽  
Susan H. Berry ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Dna Extraction ◽  
Bacterial Community Structure ◽  
Fecal Samples ◽  
The Impact

Heterotrophic Bacterial Community Structure of Multistage Biofilters in a Commercial Pufferfish Takifugu rubripes RAS

2013 ◽  
Vol 726-731 ◽  
pp. 1621-1627 ◽  
Author(s):  
Zhu Chen ◽  
Ying Liu ◽  
Liang Zi Liu ◽  
Xiao Jing Wang ◽  
Zhi Pei Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Diversity Index ◽  
Community Diversity ◽  
Takifugu Rubripes ◽  
Recirculating Aquaculture ◽  
Microbial Resources

The success of a recirculating aquaculture system (RAS) greatly depends on the structure, dynamics and activities of microbial community. Heterotrophic bacteria as the major members play various roles. The heterotrophic bacterial community structure in threestaged biofilters was studied using four different media. 228 isolates belonging to 77species were obtained and affiliated toGammaproteobacteria,Alphaproteobacteria,Bacteroidetes,Firmicutes,ActinobacteriaandBetaproteobacteria.Gammaproteobacteriawas the predominant group. The concurrence was found between potential pathogens (VibrioandShewanella) and probiotics (BacillusandPseudomonas). On the basis of community diversity index, we could infer that differences existed between stages, and the diversity index increased along the biofilters. A comprehensive understanding of microbial community in RAS will be in favor of utilization of microbial resources and optimizing the culture systems' operation.

scholarly journals The impact of host rock geochemistry on bacterial community structure in oligotrophic cave environments

2007 ◽  
Vol 36 (2) ◽  
pp. 93-104 ◽  
Author(s):  
Hazel Barton ◽  
Nicholas Taylor ◽  
Michael Kreate ◽  
Austin Springer ◽  
Stuart Oehrle ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Host Rock ◽  
Bacterial Community Structure ◽  
The Impact

scholarly journals Metagenomic Exploration of Bacterial Community Structure of Earthworms’ Gut

2021 ◽  
Author(s):  
Samrendra Singh Thakur ◽  
Azhar Rashid Lone ◽  
Nalini Tiwari ◽  
Subodh Kumar Jain ◽  
Shweta Yadav
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Gut Microbiota ◽  
Bacterial Community Structure ◽  
Alpha Diversity ◽  
Eudrilus Eugeniae ◽  
Phenotypic Analysis ◽  
Perionyx Excavatus ◽  
Gram Negative ◽  
Earthworm Species

Living organisms are naturally bestowed with unique and imitable qualities for maintaining ecological balance and earthworms are no exceptions. These so-called keystone species of terrestrial ecosystems are equipped with wonderful machinery, allowing them to nurture soil beautifully. Earthworm gut represents a potential microbial reservoir, having a complex interdependence with the host. The study aimed to profile bacterial community structure of three earthworm species belonging to two different life forms; Perionyx excavatus and Eudrilus eugeniae (epigeic), Polypheretima elongata (endogeic) respectively. Diversity analysis using 16S amplicon sequencing revealed that the dominant phyla were Proteobacteria (34.17-77.88) followed by Actinobacteria (13.43-35.54%), Firmicutes (1.69-15.45%) and Bacteroidetes (0.51-8.12%). The alpha diversity indices explicit similar gut microbiota of Perionyx excavatus and Eudrilus eugeniae and while higher alpha diversity was recorded in comparison to Polypheretima elongata gut. The taxonomic to the phenotypic annotation of 16S rRNA metagenomes revealed that dominance of Gram-negative bacterial community in all earthworm species while, Polypheretima elongata comprises higher percentage (78%) of Gram-negative bacterial community to Perionyx excavatus (32.3%) and Eudrilus eugeniae (38.3%). The oxygen requirement phenotypic analysis showed that all earthworm species were abundant with aerobic followed by anaerobic bacterial groups. Furthermore, functional metabolism phenotypic analysis revealed that a high abundance of ammonia oxidizers (29.3-80.2%), the gut microbiomes showed the relative abundance of sulphate reducer (22.6-78.7%), nitrite reducer (19.8-73.2%), dehalogenators (12.6-25.1%), illustrating in the role of these microbial communities in various degradation and bioremediation processes. The present study signifies the intrinsic gut microbiota of earthworm species for intensified biodegradation.

scholarly journals Responses of Soil Organic Carbon Sequestration Potential and Bacterial Community Structure in Moso Bamboo Plantations to Different Management Strategies in Subtropical China

Forests ◽  
2018 ◽  
Vol 9 (10) ◽  
pp. 657 ◽  
Author(s):  
Chuanbao Yang ◽  
Zheke Zhong ◽  
Xiaoping Zhang ◽  
Fangyuan Bian ◽  
Xuhua Du
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Management Strategies ◽  
Moso Bamboo ◽  
Mineralization Rate ◽  
Bamboo Forest ◽  
C Mineralization ◽  
Sequestration Potential ◽  
The Impact

Moso bamboo is one of the fastest-growing plants in the world. The objective of this study was to investigate the impact of converting secondary broadleaf evergreen forests (CK) to Moso bamboo plantations, and the impact of different management strategies, including no disturbance (M0), extensive management (M1), and intensive management (M2), on the soil organic carbon (SOC) sequestration potential, and relevant characteristics of the soil bacterial community. Our results showed that, in comparison with CK, M0 and M1 had significantly higher SOC and recalcitrant organic materials (aliphatic and aromatic compounds), and a lower C mineralization rate, whereas M2 had the opposite effects. The conversion from CK to Moso bamboo plantation significantly decreased the relative abundance of Acidobacteria in both the topsoil and subsoil soil layers. Compared with CK, M0 led to the enrichment of bacteria such as Alphaproteobacteria, Chloroflexi, and Bacteroidetes, which are involved in the decomposition of organic matter and the formation of humus and are, therefore, potentially beneficial for increasing the SOC. Furthermore, the ratio of the microbial biomass C (MBC) to total organic C (TOC), C mineralization rate, and bacterial diversity increased from M0 to M2, i.e., with an increase in the disturbance intensity. These findings indicate that the conversion of secondary broadleaf forest to bamboo forest alter the soil bacterial community structure. Reducing disturbance in bamboo forest management strategies should be actively taken up to improve the SOC, and maintain sustainable development in the forest industry.

Nutrient availability and organic matter quality shape bacterial community structure in a lake biofilm

2020 ◽  
Vol 85 ◽  
pp. 1-18
Author(s):  
RC Seballos ◽  
KH Wyatt ◽  
RJ Bernot ◽  
SP Brown ◽  
S Chandra ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Interspecific Interactions ◽  
Molecular Techniques ◽  
Nitrogen And Phosphorus ◽  
Microbial Composition ◽  
Organic Matter Quality

Heterotrophic bacteria play a key role in ecosystem processes, but little is known about the factors that shape bacterial community structure in aquatic biofilms, especially in lakes. We used molecular techniques (16S rRNA) to evaluate resource controls on biofilm bacterial community structure in an oligotrophic subalpine lake. We manipulated nutrients (nitrogen and phosphorus; NP) and glucose (G) on inorganic (rock) and organic (wood) substrates under light and dark conditions (i.e. with and without autotrophy, respectively) in a full factorial design using nutrient diffusing substrates in situ for 20 d. Distinct patterns of separation in community structure between treatments with nutrients (NP, NP+G) and without nutrients (control, G-only) indicated that community structure was more strongly influenced by nutrients than organic matter irrespective of substrate type or light availability. Further separation in community structure between treatments with nutrients only (NP) and nutrients with glucose (NP+G) on both organic and inorganic substrates indicated that once nutrient limitation was alleviated, organic matter quality played an important role in shaping community structure. Differences in the relative abundance of 6 phyla, 3 classes, and 19 genera among treatments revealed (1) contrasting taxa-specific resource requirements, (2) the influence of interspecific interactions on composition, and (3) the potential for individual taxa to participate in the decomposition of recalcitrant organic matter. Our findings provide insight into the role that nutrients and organic matter quality play in shaping bacterial community structure, which is a critical step in bridging the knowledge gap between microbial composition and ecosystem function within aquatic environments.

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