Nutrient availability and organic matter quality shape bacterial community structure in a lake biofilm

2020 ◽  
Vol 85 ◽  
pp. 1-18
Author(s):  
RC Seballos ◽  
KH Wyatt ◽  
RJ Bernot ◽  
SP Brown ◽  
S Chandra ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Interspecific Interactions ◽  
Molecular Techniques ◽  
Nitrogen And Phosphorus ◽  
Microbial Composition ◽  
Organic Matter Quality

Heterotrophic bacteria play a key role in ecosystem processes, but little is known about the factors that shape bacterial community structure in aquatic biofilms, especially in lakes. We used molecular techniques (16S rRNA) to evaluate resource controls on biofilm bacterial community structure in an oligotrophic subalpine lake. We manipulated nutrients (nitrogen and phosphorus; NP) and glucose (G) on inorganic (rock) and organic (wood) substrates under light and dark conditions (i.e. with and without autotrophy, respectively) in a full factorial design using nutrient diffusing substrates in situ for 20 d. Distinct patterns of separation in community structure between treatments with nutrients (NP, NP+G) and without nutrients (control, G-only) indicated that community structure was more strongly influenced by nutrients than organic matter irrespective of substrate type or light availability. Further separation in community structure between treatments with nutrients only (NP) and nutrients with glucose (NP+G) on both organic and inorganic substrates indicated that once nutrient limitation was alleviated, organic matter quality played an important role in shaping community structure. Differences in the relative abundance of 6 phyla, 3 classes, and 19 genera among treatments revealed (1) contrasting taxa-specific resource requirements, (2) the influence of interspecific interactions on composition, and (3) the potential for individual taxa to participate in the decomposition of recalcitrant organic matter. Our findings provide insight into the role that nutrients and organic matter quality play in shaping bacterial community structure, which is a critical step in bridging the knowledge gap between microbial composition and ecosystem function within aquatic environments.

Heterotrophic Bacterial Community Structure of Multistage Biofilters in a Commercial Pufferfish Takifugu rubripes RAS

2013 ◽  
Vol 726-731 ◽  
pp. 1621-1627 ◽  
Author(s):  
Zhu Chen ◽  
Ying Liu ◽  
Liang Zi Liu ◽  
Xiao Jing Wang ◽  
Zhi Pei Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Heterotrophic Bacteria ◽  
Diversity Index ◽  
Community Diversity ◽  
Takifugu Rubripes ◽  
Recirculating Aquaculture ◽  
Microbial Resources

The success of a recirculating aquaculture system (RAS) greatly depends on the structure, dynamics and activities of microbial community. Heterotrophic bacteria as the major members play various roles. The heterotrophic bacterial community structure in threestaged biofilters was studied using four different media. 228 isolates belonging to 77species were obtained and affiliated toGammaproteobacteria,Alphaproteobacteria,Bacteroidetes,Firmicutes,ActinobacteriaandBetaproteobacteria.Gammaproteobacteriawas the predominant group. The concurrence was found between potential pathogens (VibrioandShewanella) and probiotics (BacillusandPseudomonas). On the basis of community diversity index, we could infer that differences existed between stages, and the diversity index increased along the biofilters. A comprehensive understanding of microbial community in RAS will be in favor of utilization of microbial resources and optimizing the culture systems' operation.

Relationships between Soil Organic Matter, Nutrients, Bacterial Community Structure, And the Performance of Microbial Fuel Cells

2012 ◽  
Vol 46 (3) ◽  
pp. 1914-1922 ◽  
Author(s):  
Sara J. Dunaj ◽  
Joseph J. Vallino ◽  
Mark E. Hines ◽  
Marcus Gay ◽  
Christine Kobyljanec ◽  
...  
Keyword(s):  
Organic Matter ◽  
Fuel Cells ◽  
Community Structure ◽  
Soil Organic Matter ◽  
Bacterial Community ◽  
Microbial Fuel Cells ◽  
Bacterial Community Structure

scholarly journals Bacterial community structure in the Bohai Strait provides insights into organic matter niche partitioning

2018 ◽  
Vol 169 ◽  
pp. 46-54 ◽  
Author(s):  
Yibo Wang ◽  
Bin Wang ◽  
Lisa M. Dann ◽  
James G. Mitchell ◽  
Xiaoke Hu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Niche Partitioning ◽  
Bohai Strait

scholarly journals Bacterial community structure and dissolved organic matter in repeatedly flooded subsurface karst water pools

2014 ◽  
Vol 89 (1) ◽  
pp. 111-126 ◽  
Author(s):  
Tanja Shabarova ◽  
Jörg Villiger ◽  
Oleg Morenkov ◽  
Jutta Niggemann ◽  
Thorsten Dittmar ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Bacterial Community Structure ◽  
Karst Water

Effect of earthworm loads on organic matter and nutrient removal efficiencies in synthetic domestic wastewater, and on bacterial community structure and diversity in vermifiltration

2013 ◽  
Vol 68 (1) ◽  
pp. 43-49 ◽  
Author(s):  
L. M. Wang ◽  
X. Z. Luo ◽  
Y. M. Zhang ◽  
J. J. Lian ◽  
Y. X. Gao ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Removal Rate ◽  
Oxygen Demand ◽  
Domestic Wastewater ◽  
Ammonia Nitrogen ◽  
Domestic Sewage ◽  
Shannon Index

In this paper, we studied the effect of earthworm loads on the removal rates of chemical oxygen demand (COD), ammonia nitrogen (NH3-N), total nitrogen, and total phosphorus from synthetic domestic sewage and on the bacterial community structure and diversity of substrates in earthworm packing beds. The different vermifiltrations (VFs), including the control, are successful in removing both organic matter (OM) and nutrients. The removal rate of NH3-N at 12.5 g of earthworm/L of soil VF is higher compared with that at 0 and 4.5 earthworm load VFs. The highest Shannon index, in the earthworm packing bed, occurred at 16.5 earthworm load VF. Furthermore, the COD removal rate is significantly correlated with the Shannon index, which reveals that OM removal for synthetic domestic sewage treatment at VF might be more dependent on bacterial diversity at the earthworm packing bed. The band distributions and diversities of the bacterial community for samples from different earthworm loads in VFs suggest that the bacterial community structure was only affected within the earthworm packing bed when the earthworm load reached a certain level. The present study adds to the current understanding of OM and nutrient degradation processes in VF domestic wastewater treatment.

scholarly journals Effects of enrichmemt planting with native tree species on bacterial community structure and potential impact on Eucalyptus plantations in southern China

2022 ◽  
Author(s):  
Yuxing Xu ◽  
Chao Li ◽  
Wankuan Zhu ◽  
Zhichao Wang ◽  
Lichao Wu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Third Generation ◽  
The Third ◽  
Α Diversity ◽  
Native Tree ◽  
Native Tree Species ◽  
Mixed Systems

AbstractMulti-generational planting of Eucalyptus species degrades soil quality but the introduction of legumes can improve soil fertility and microbial diversity. However, the effects of introducing non-legume native tree species on soil nutrients and bacterial community structure remain poorly understood. This study investigated the impacts of the conversion of third generation monoculture Eucalyptus plantations to mixed systems including Eucalyptus urograndis with Cinnamomum camphora (EC) and E. urograndis with Castanopsis hystrix (EH), on soil chemical and biochemical properties and bacterial community structure, diversity and functions. First generation E. urophylla plantations were the control. Results show that planting the third generation Eucalyptus led to a significant decrease in pH, organic matter, nutrient content, enzyme activities (invertin, acid phosphataes, and urease), and bacterial α-diversity compare to the controls. However, the mixed planting showed significant improvement in soil chemical and biochemical attributes and bacterial α-diversity, although the E. urograndis and C. hystrix planting had no improvement. Chloroflexi (oligotrophic bacteria) were significantly enriched in third generation Eucalyptus and Eucalyptus + C. hystrix, while proteobacteria increased significantly in the E. urograndis with C. camphora plantings. The relative abundance of multiple metabolic pathways increased significantly in the third generation Eucalyptus plantations whereas membrane transport-related genes were enriched in soils of the mixed systems. The changes in bacterial community structures in the two mixed systems were driven by diversity, organic matter and acid phosphatase, while bacterial functions were affected by invertase, $${\mathrm{NO}}_{3}^{-}$$ NO 3 - -N, diversity and urease. These results suggest that the transformation of successive monoculture Eucalyptus plantations into mixed plantations reduces the depletion of soil nutrients and enhances the ecological function of soil microorganisms.

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