The use of babassu oil as substrate to produce bioemulsifiers byCandida lipolytica

1999 ◽  
Vol 45 (5) ◽  
pp. 423-426 ◽  
Author(s):  
L A Sarubbo ◽  
ALF Porto ◽  
G M Campos-Takaki

Candida lipolytica IA 1055 produced an extracellular emulsifier when using babassu oil as its sole carbon source during batch and fed batch fermentations at 27°C. Emulsification activity was detected after 60 h of growth in all conditions studied. The bioemulsifier was isolated after 144 h of fermentation from the best condition studied. The biopolymer seems to be a polysaccharide-protein-lipid complex.Key words: bioemulsifiers, biopolymer, Candida lipolytica, babassu oil, fermentation.

2020 ◽  
Vol 7 (4) ◽  
pp. 138
Author(s):  
Spyridon Achinas ◽  
Sytse Jelmer Mulder ◽  
Gerrit Jan Willem Euverink

Syngas fermentation by methanogens is a novel process to purify biogas. Methanogens are able to ferment non-desirable CO2, H2, and CO to methane. However, to use methanogens on an industrial scale, more research has to be done. There are studies that discuss the growth of methanogens on syngas in combination with acetate. In this research, growth of methanogens on syngas as sole carbon source is discussed. Effluent of an anaerobic fed-batch was selectively cultivated with syngas in 400 mL Eppendorf© bioreactors. After a period of 7 days, fifteen 120 mL flasks were filled with three different liquid-to-gas ratios (1:1, 1:3, 1:5). Results showed that different liquid-to-gas ratios change the metabolic preference of the anaerobic microbial community. Moreover, complete conversion in a four-to-eight-day period, via the carboxidotrophic pathway, was observed in all three liquid-to-gas ratios.


2021 ◽  
Author(s):  
Alireza Habibi ◽  
Samira Fallahi ◽  
Saeed Abbasi ◽  
Rouhallah Sharifi

Abstract Application of agro-industrial waste in microbial fermentation is interesting in economic and environmental aspects. Carotenoids production by Rhodotorula toruloides KP324973 was investigated using corn steep liquor (CSL) as sole carbon source. Haldane model with constants µmax = 0.056 h-1, KS = 1.54 vv-1%, and KI = 58.58 vv-1% showed best describe of cell growth kinetics on CLS. A same maximum carotenoid production rate (Rp) about 2.23 μg gcell-1 h-1 was obtained at initial CSL concentration of 5 v/v% after 72 h and 21 h in batch cultivation in shaken flasks and bubble column reactor (BCR), respectively. Further improvement of carotenogenesis was followed by fed-batch cultivation in BCR where the optimal setting of factors at feed flow rate of 5 mL h-1, pH of 5.66, and temperature 14 ˚C gained a highest Rp = 8.686 μg gcell-1 h-1. Chromatographic analysis showed more than 94% of produced carotenes was β-carotene.


Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  

1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.


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