Brewing spoilage lactobacilli detected using monoclonal antibodies to bacterial surface antigens

1999 ◽  
Vol 45 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Michael S Whiting ◽  
Sheryl L Gares ◽  
W M Ingledew ◽  
Barry Ziola
Keyword(s):  
Monoclonal Antibodies ◽  
Surface Antigen ◽  
Environmental Conditions ◽  
Surface Antigens ◽  
Specific Detection ◽  
Bacterial Surface ◽  
Protease Treatment

A panel of thirteen monoclonal antibodies (Mabs) was assembled that reacts with surface antigens on eight of eleven Lactobacillus brewing spoilage organisms, including one or more of L. brevis, L. buchneri, L. casei-alactosus, L. plantarum, or unspeciated isolate(s). Immunoblotting was done to identify the antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment and by surface antigen extraction of washed bacteria. Protease susceptibility of extracted surface antigens was also examined. In most cases, Lactobacillus surface antigens detected by the Mabs appear to be noncovalently bound proteins readily altered or removed from the bacterium by various environmental conditions. This research identifies brewing conditions that need to be tested to ascertain whether bacterial surface antigen-reactive Mabs can be used for the rapid, sensitive, and specific detection of Lactobacillus brewing spoilage organisms.Key words: brewing spoilage organism, immunoassay, Lactobacillus, monoclonal antibodies, surface antigens.

Bacterial surface antigen-specific monoclonal antibodies used to detect beer spoilage pediococci

1999 ◽  
Vol 45 (8) ◽  
pp. 670-677 ◽  
Author(s):  
Michael S Whiting ◽  
W M Ingledew ◽  
Sun Y Lee ◽  
Barry Ziola
Keyword(s):  
Cell Wall ◽  
Monoclonal Antibodies ◽  
Surface Antigen ◽  
Surface Antigens ◽  
Bacterial Surface ◽  
Cell Wall Polymers ◽  
Good Potential ◽  
Protease Treatment ◽  
Beer Spoilage

Fourteen monoclonal antibodies (Mabs) were isolated that react with surface antigens of Pediococcus beer spoilage organisms, including P. damnosus, P. pentosaceous, P. acidilactici, and unspeciated isolates. Immunoblotting, enzyme immunoassays (EIAs) of protease- and neuraminidase-treated surface antigen extracts, carbohydrate competition EIAs, and cardiolipin EIAs were used to characterize the bacterial antigens involved in Mab binding. Antigen stability in situ was tested by protease treatment or surface antigen extraction of washed bacteria. In most cases, the Mabs bind to Pediococcus surface antigens that appear to be covalently bound cell wall polymers resistant to alteration or removal from the bacterial surface. These bacterial surface antigen reactive Mabs show good potential for rapid, sensitive, and specific immunoassay detection of Pediococcus beer spoilage organisms.Key words: beer spoilage organism, immunoassay, monoclonal antibodies, Pediococcus, surface antigens.

The occurrence of antibodies to hidden and exposed determinants of surface antigens of Trichinella spiralis

Parasitology ◽  
1984 ◽  
Vol 88 (2) ◽  
pp. 359-369 ◽  
Author(s):  
Guadalupe Ortega-Pierres ◽  
Ann Chayen ◽  
N. W. T. Clark ◽  
R. M. E. Parkhouse
Keyword(s):  
Monoclonal Antibodies ◽  
Surface Antigen ◽  
Developmental Stages ◽  
Trichinella Spiralis ◽  
Surface Antigens ◽  
Myeloma Cell ◽  
Molecular Heterogeneity ◽  
Major Surface ◽  
Polypeptide Structure

SUMMARYMice were infected per os with Trichinella spiralis and their lymphocytes were removed and fused with mouse myeloma cell line P3 × 63Ag8653P3 for the selection of monoclonal antibodies to biochemically defined, stage-specific surface antigens of 3 parasite developmental stages: muscle larvae, adults and newborn larvae. Two separate antibodies against a defined single surface antigen of each stage were isolated. In each separate case the pair of monoclonal antibodies precipitated the same component from detergent-solubilized surface antigen preparations, but only one was able to bind to the surface of the living worm. The other must therefore be directed against an antigenic epitope which is obscured in the intact worm surface. The latter type of antibody is unlikely to be involved in the initial phase of parasite rejection and hence is another example of a non-protective host antibody response. The stimulus for its synthesis may be release of surface antigen, which does occur in vitro. One surface antigen of the newborn larvae is only detected by antibody in the first 6 h after birth; thereafter its presence is obscured as other antigens appear. The major surface antigen of the infective larvae contains carbohydrate determinants which are not available at the parasite surface. In addition, it displays great molecular heterogeneity but all variants appear to be derived from a common polypeptide structure.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

MixAR

2019 ◽  
Vol 12 (4) ◽  
pp. 1-33 ◽  
Author(s):  
Telmo Adão ◽  
Luís Pádua ◽  
David Narciso ◽  
Joaquim João Sousa ◽  
Luís Agrellos ◽  
...  
Keyword(s):  
Environmental Conditions ◽  
Mixed Reality ◽  
The Real ◽  
Points Of Interest ◽  
Real Scene ◽  
The Future

MixAR, a full-stack system capable of providing visualization of virtual reconstructions seamlessly integrated in the real scene (e.g. upon ruins), with the possibility of being freely explored by visitors, in situ, is presented in this article. In addition to its ability to operate with several tracking approaches to be able to deal with a wide variety of environmental conditions, MixAR system also implements an extended environment feature that provides visitors with an insight on surrounding points-of-interest for visitation during mixed reality experiences (positional rough tracking). A procedural modelling tool mainstreams augmentation models production. Tests carried out with participants to ascertain comfort, satisfaction and presence/immersion based on an in-field MR experience and respective results are also presented. Ease to adapt to the experience, desire to see the system in museums and a raised curiosity and motivation contributed as positive points for evaluation. In what regards to sickness and comfort, the lowest number of complaints seems to be satisfactory. Models' illumination/re-lightning must be addressed in the future to improve the user's engagement with the experiences provided by the MixAR system.

scholarly journals Specificity and VH sequence of two monoclonal antibodies against the N-terminus of dystrophin

1995 ◽  
Vol 309 (1) ◽  
pp. 355-359 ◽  
Author(s):  
G E Morris ◽  
C Nguyen ◽  
Nguyen thi Man
Keyword(s):  
Monoclonal Antibodies ◽  
Point Mutations ◽  
Hypervariable Region ◽  
Variable Region ◽  
Binding Studies ◽  
Cdna Sequences ◽  
N Terminus ◽  
Random Library ◽  
Blast Cells

We have used a random library of 15-mer peptides expressed on phage to show that two monoclonal antibodies (mAbs) require only the first three amino acids of dystrophin (Leu-Trp-Trp) for binding. Since the mAbs recognize dystrophin in frozen muscle sections, the results suggest that this hydrophobic N-terminus of dystrophin is accessible to antibody in situ. Quantitative binding studies suggested minor differences in specificity between the two mAbs, so the Ig heavy-chain variable region (VH) sequences of the two hybridomas were determined by RT-PCR and cDNA sequencing. After elimination of PCR errors, the two cDNA sequences were found to be identical except for five somatic mutations which resulted in three amino acid changes in the second hypervariable region (CDR2). The results suggest that the two hybridomas originated from the same lymphocyte clone in a germinal centre of the spleen, but underwent different point mutations and subtype switches during clonal expansion to form blast cells.

scholarly journals Visual detection of granulocyte surface antigens using the avidin-biotin complex.

1984 ◽  
Vol 32 (7) ◽  
pp. 712-716 ◽  
Author(s):  
M Henke ◽  
L M Yonemoto ◽  
G S Lazar ◽  
L Gaidulis ◽  
T Hecht ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Indirect Immunofluorescence ◽  
Visual Detection ◽  
Hla Antigens ◽  
Surface Antigens ◽  
Cell Populations ◽  
Surface Markers ◽  
Visual Test ◽  
Avidin Biotin Complex

A visual test for detection of granulocyte surface markers using the avidin-biotin complex (ABC) has been developed. That this assay is highly specific, reproducible, and sensitive was determined by studying the expression of HLA antigens on granulocytes with monoclonal antibodies. Further, using granulocyte specific alloantisera, the results of the ABC test compared well to data from leukoagglutination assays and indirect immunofluorescence tests. The assay is particularly advantageous in that granulocytes can be stored, only small amounts of cells and sera are needed, and heterogeneous cell populations can easily be studied.

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