Effects of two diamine biocides on the microbial community from an oil field

1998 ◽  
Vol 44 (11) ◽  
pp. 1060-1065 ◽  
Author(s):  
Anita J Telang ◽  
Sara Ebert ◽  
Julia M Foght ◽  
Donald WS Westlake ◽  
Gerrit Voordouw
Keyword(s):  
Sulfate Reduction ◽  
Microbial Population ◽  
Oil Field ◽  
Microbial Populations ◽  
Microbial Corrosion ◽  
Broad Distribution ◽  
Sulfate Reducing ◽  
Production Water ◽  
Field Samples ◽  
Reducing Bacteria

The effects of diamine (R1-NH-R2-NH2) biocides A and B on the microbial population from an oil field were investigated with reverse sample genome probing (RSGP), a technique designed to track multiple oil field bacteria in a single assay. RSGP studies of sessile microbial populations scraped from corrosion coupons obtained from biocide-treated oil field installations indicated dominance of Desulfovibrio spp. Lac6 and Eth3, or of selected heterotrophs. RSGP of planktonic production water samples indicated a broad distribution of microorganisms that changed with the addition of medium for the growth of sulfate-reducing bacteria, containing different organic acids as electron donors for sulfate reduction. Use of lactate, propionate, or acetate enriched Desulfovibrio spp., Desulfobulbus sp. Pro4, or Desulfobacter spp., respectively. Treatment of lactate- or mixed organic acid-fed planktonic populations with biocides indicated resistance of Lac6 and Eth3 to 400 ppm of biocide B and 40 ppm of biocide A. The dominance of these two Desulfovibrio spp. in many sessile field samples is, therefore, likely caused by biocide selection.Key words: Desulfovibrio, sulfate reduction, microbial corrosion, souring, biocide.

scholarly journals The Self Ignition of Fiberglass Lines

2014 ◽  
Vol 39 ◽  
pp. 1-9
Author(s):  
Moussa Bounoughaz ◽  
Noura Touabi
Keyword(s):  
Microbial Population ◽  
Bacterial Species ◽  
Methanogenic Bacteria ◽  
Oil Field ◽  
Microbiological Analysis ◽  
High Concentration ◽  
Sulfate Reducing ◽  
Maintenance Work ◽  
Reducing Bacteria

The fiberglass pipes were used for transportation of water in order to maintain the pressure in the oil field of Zarzaïtine (Region of In Amenas located in the south of Algeria). During the maintenance work in summer, a season well known for its extensive heat, the deposits contained in the pipe, and after its contact with the atmosphere, have caused a smoke and it was followed by a fire that ignited a portion of the pipe. To give an answer to the causes of this phenomenon, we have first incriminated the role of specific bacterial species and therefore we have made a microbiological analysis of the deposits collected from the site of the incident. The obtained results revealed the presence of a heterogeneous microbial population with a high concentration level of sulfate-reducing bacteria (SRB), methanogenic bacteria (MB), yeasts and fungi. The interaction between the different species of bacteria and the organic matter contained in the deposits has generated the formation of methane which under the influence of the great heat burnt and the fire caused the ignition of the fiberglass line.

scholarly journals Application of Denaturing High-Performance Liquid Chromatography for Monitoring Sulfate-Reducing Bacteria in Oil Fields

2013 ◽  
Vol 79 (17) ◽  
pp. 5186-5196 ◽  
Author(s):  
Outi Priha ◽  
Mari Nyyssönen ◽  
Malin Bomberg ◽  
Arja Laitila ◽  
Jaakko Simell ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Content Type ◽  
Sulfate Reducing ◽  
Microbially Induced Corrosion ◽  
Field Samples ◽  
Reducing Bacteria

ABSTRACTSulfate-reducing bacteria (SRB) participate in microbially induced corrosion (MIC) of equipment and H2S-driven reservoir souring in oil field sites. Successful management of industrial processes requires methods that allow robust monitoring of microbial communities. This study investigated the applicability of denaturing high-performance liquid chromatography (DHPLC) targeting the dissimilatory sulfite reductase ß-subunit (dsrB) gene for monitoring SRB communities in oil field samples from the North Sea, the United States, and Brazil. Fifteen of the 28 screened samples gave a positive result in real-time PCR assays, containing 9 × 101to 6 × 105dsrBgene copies ml−1. DHPLC and denaturing gradient gel electrophoresis (DGGE) community profiles of the PCR-positive samples shared an overall similarity; both methods revealed the same samples to have the lowest and highest diversity. The SRB communities were diverse, and differentdsrBcompositions were detected at different geographical locations. The identifieddsrBgene sequences belonged to several phylogenetic groups, such asDesulfovibrio,Desulfococcus,Desulfomicrobium,Desulfobulbus,Desulfotignum,Desulfonatronovibrio, andDesulfonauticus. DHPLC showed an advantage over DGGE in that the community profiles were very reproducible from run to run, and the resolved gene fragments could be collected using an automated fraction collector and sequenced without a further purification step. DGGE, on the other hand, included casting of gradient gels, and several rounds of rerunning, excising, and reamplification of bands were needed for successful sequencing. In summary, DHPLC proved to be a suitable tool for routine monitoring of the diversity of SRB communities in oil field samples.

Identification and Phylogenetic Analysis of Thermophilic Sulfate-Reducing Bacteria in Oil Field Samples by 16S rDNA Gene Cloning and Sequencing

Anaerobe ◽  
1998 ◽  
Vol 4 (3) ◽  
pp. 165-174 ◽  
Author(s):  
Jyh-Yih Leu ◽  
Caroline P McGovern-Traa ◽  
Andrew J.R Porter ◽  
William J Harris ◽  
W.Allan Hamilton
Keyword(s):  
Phylogenetic Analysis ◽  
Gene Cloning ◽  
16S Rdna ◽  
Oil Field ◽  
Cloning And Sequencing ◽  
16S Rdna Gene ◽  
Rdna Gene ◽  
Sulfate Reducing ◽  
Field Samples ◽  
Reducing Bacteria

Trialing wetlands to treat coal mining wastewaters in a low rainfall, high evaporation environment

1997 ◽  
Vol 35 (5) ◽  
pp. 293-299 ◽  
Author(s):  
Wendy R. Tyrrell ◽  
David R. Mulligan ◽  
Lindsay I. Sly ◽  
L. Clive Bell
Keyword(s):  
Sulfate Reduction ◽  
Coal Mining ◽  
Pilot Scale ◽  
Cattle Manure ◽  
Annual Rainfall ◽  
Sulfate Reducing ◽  
Black Precipitate ◽  
Final Treatment ◽  
Reducing Bacteria ◽  
Initial Results

The large number of wetlands treating mining wastewaters around the world have mostly been constructed in temperate environments. Wetlands have yet to be proven in low rainfall, high evaporation environments and such conditions are common in many parts of Australia. BHP Australia Coal is researching whether wetlands have potential in central Queensland to treat coal mining wastewaters. In this region, mean annual rainfall is < 650 mm and evaporation > 2 000 mm. A pilot-scale wetland system has been constructed at an open-cut coal mine. The system comprises six treatment cells, each 125 m long and 10 m wide. The system is described in the paper and some initial results presented. Results over the first fourteen months of operation have shown that although pH has not increased enough to enable reuse or release of the water, sulfate reduction has been observed in parts of the system, as shown by the characteristic black precipitate and smell of hydrogen sulfide emanating from the wetlands. These encouraging signs have led to experiments aimed at identifying the factors limiting sulfate reduction. The first experiment, described herein, included four treatments where straw was overlain by soil and the water level varied, being either at the top of the straw, at the top of the soil, or about 5 cm above the soil. The effect of inoculating with sulfate-reducing bacteria was investigated. Two controls were included, one covered and one open, to enable the effect of evaporation to be determined. The final treatment consisted of combined straw/cattle manure overlain with soil. Results showed that sulfate reduction did occur, as demonstrated by pH increases and lowering of sulfate levels. Mean pH of the water was significantly higher after 19 days; in the controls, pH was < 3.3, whereas in the treatments, pH ranged from 5.4 to 6.7. The best improvement in sulfate levels occurred in the straw/cattle manure treatment.

Microbial ecology of sulfate-reducing bacteria in wastewater biofilms analyzed by microelectrodes and fish (fluorescent in situ hybridization) technique

1999 ◽  
Vol 39 (7) ◽  
pp. 41-47 ◽  
Author(s):  
Satoshi Okabe ◽  
Hisashi Satoh ◽  
Tsukasa Itoh ◽  
Yoshimasa Watanabe
Keyword(s):  
In Situ Hybridization ◽  
Sulfate Reduction ◽  
Sulfate Reducing Bacteria ◽  
Hybridization Technique ◽  
Concentration Profiles ◽  
Reduction Zone ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Culture Dependent

The vertical distribution of sulfate-reducing bacteria (SRB) in microaerophilic wastewater biofilms grown on fully submerged rotating disk reactors (RDR) was determined by the conventional culture-dependent MPN method and in situ hybridization of fluorescently-labelled 16S rRNA-targeted oligonucleotide probes for SRB in parallel. Chemical concentration profiles within the biofilm were also measured using microelectrodes for O2, S2-, NO3- and pH. In situ hybridization revealed that the SRB probe-stained cells were distributed throughout the biofilm even in the oxic surface zone in all states from single scattered cells to clustered cells. The higher fluorescence intensity and abundance of SRB probe-stained cells were found in the middle part of the biofilm. This result corresponded well with O2 and H2S concentration profiles measured by microelectrodes, showing sulfate reduction was restricted to a narrow anaerobic zone located about 500 μm below the biofilm surface. Results of the MPN and potential sulfate reducing activity (culture-dependent approaches) indicated a similar distribution of cultivable SRB in the biofilm. The majority of the general SRB probe-stained cells were hybridized with SRB 660 probe, suggesting that one important member of the SRB in the wastewater biofilm could be the genus Desulfobulbus. An addition of nitrate forced the sulfate reduction zone deeper in the biofilm and reduced the specific sulfate reduction rate as well. The sulfate reduction zone was consequently separated from O2 and NO3- respiration zones. Anaerobic H2S oxidation with NO3- was also induced by addition of nitrate to the medium.

scholarly journals Green biocide for mitigating sulfate-reducing bacteria influenced microbial corrosion

3 Biotech ◽  
2018 ◽  
Vol 8 (12) ◽  
Author(s):  
Shanthi Vaithiyanathan ◽  
Karthikeyan Chandrasekaran ◽  
R. C. Barik
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Microbial Corrosion ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Corrosion Behaviors of 0Cr18Ni9 Stainless Steel under the Combination Action of Sulfate-Reducing Bacteria and V.natriegens

2011 ◽  
Vol 356-360 ◽  
pp. 161-164
Author(s):  
Cai Xiang Gu ◽  
Xiao Ming Zhao ◽  
Yan Sheng Yin ◽  
Gui Jun Ji
Keyword(s):  
Stainless Steel ◽  
Corrosion Potential ◽  
Sea Water ◽  
Microbial Corrosion ◽  
Sulfate Reducing ◽  
Facultative Anaerobic ◽  
Corrosion Behaviors ◽  
Microbial Film ◽  
Combined Action ◽  
Reducing Bacteria

Advantage strains SRB and V.natriegens were obtained from the China East Sea for this study. Polarization curves, corrosion potential, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) analyses were adopted in order to investigate the corrosion behaviors of 0Cr18Ni9 stainless steel under the combination action of anaerobic SRB and facultative anaerobic V.natriegens, The characteristics and mechanisms of microbial corrosion action in sea water were analyzed in this paper. The results show that SRB and V.natriegens promote each other’s growth when cultivated in the mixed microbe medium, in which the rate of corrosion is higher than that in the single microbe; Under the combined action of the mixed microbe, the microbial film gets wider and thicker, and corrosion products and metabolite are produced, which furthermore accelerates the passivation and pit corrosion to the 0Cr18Ni9 stainless steel.

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