Conversion of dibenzothiophene to biphenyl by sulfate-reducing bacteria isolated from oil field production facilities

1995 ◽  
Vol 17 (10) ◽  
pp. 1133-1136 ◽  
Author(s):  
S. M. Armstrong ◽  
B. M. Sankey ◽  
G. Voordouw
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Field Production ◽  
Production Facilities

Characterization of the diversity of sulfate-reducing bacteria in soil and mining waste water environments by nucleic acid hybridization techniques

1994 ◽  
Vol 40 (11) ◽  
pp. 955-964 ◽  
Author(s):  
Anita J. Telang ◽  
Gerrit Voordouw ◽  
Sara Ebert ◽  
Neili Sifeldeen ◽  
Julia M. Foght ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Bacterial Communities ◽  
Genomic Dna ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Nucleic Acid Hybridization ◽  
Gene Probe ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Field Production

Nucleic acid hybridization techniques were used to characterize the sulfate-reducing bacterial communities at seven waste water and two soil sites in Canada. Genomic DNA was obtained from liquid enrichment cultures of samples taken from these nine sites. The liquid enrichment protocol favored growth of the sulfate-reducing bacterial component of the communities at these sites. The genomic DNA preparations were analyzed with (i) a specific gene probe aimed at a single genus (Desulfovibrio), (ii) a general 16S rRNA gene probe aimed at all genera of sulfate-reducing bacteria and other bacteria, and (iii) whole genome probes aimed at specific bacteria. This three-pronged approach provided information on the sulfate-reducing bacterial community structures for the nine sites. These were compared with each other and with the sulfate-reducing bacterial communities of western Canadian oil field production waters, studied previously. It was found that there is considerable diversity in the sulfate-reducing bacterial community at each site. Most sulfate-reducing bacteria isolated from distinct sites are genomically different and differ also from sulfate-reducing bacteria found in oil field production waters.Key words: sulfate-reducing bacteria, genomic diversity, nucleic acid hybridization, microbial community.

Characterization of sulfate-reducing bacteria isolated from oil-field waters

1996 ◽  
Vol 42 (3) ◽  
pp. 259-266 ◽  
Author(s):  
C. Tardy-Jacquenod ◽  
P. Caumette ◽  
R. Matheron ◽  
C. Lanau ◽  
O. Arnauld ◽  
...  
Keyword(s):  
Physicochemical Parameters ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Oil Fields ◽  
Gulf Of Guinea ◽  
Sulfate Reducing ◽  
The North ◽  
The North Sea ◽  
Reducing Bacteria

The occurrence and metabolic capacities of sulfate-reducing bacteria (SRB) were studied in 23 water samples taken from producing wells at 14 different sites. Oil fields in France, the North Sea, and the Gulf of Guinea were selected and classified according to physicochemical parameters (salinity ranging from 0.3 to 120 g∙L−1 and temperature between 29 and 85 °C). After the distribution of SRB within oil fields was studied, several strains of SRB were isolated and characterized metabolically. Twenty of the thirty-seven strains were not related to any known species. Most of the identified strains were members of the genera Desulfovibrio and Desulfotomaculum by molecular, morphological, and physiological properties.Key words: sulfate-reducing bacteria, oil-field ecology, metabolic identification, biodiversity.

scholarly journals Crude oil-utilizing strain Desulfovibrio vulgaris D107G3, a mesophilic sulfate-reducing bacterium isolated from Bach Ho gas-oil field in Vung Tau, Vietnam

2020 ◽  
Vol 12 (2) ◽  
pp. 193-199
Author(s):  
Thi Thu Huyen NGUYEN ◽  
Thi Kim Thoa TRAN ◽  
Thuy Hien LAI
Keyword(s):  
16S Rrna ◽  
Crude Oil ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Metal Corrosion ◽  
Desulfovibrio Vulgaris ◽  
Viet Nam ◽  
Gas Oil ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Some of anaerobic, mesophilic sulfate-reducing bacteria that produce H2S and cause microbial metal corrosion can degrade crude oil in anaerobic conditions. In this study, a mesophilic sulfate-reducing bacterial strain D107G3 isolated from Bach Ho gas-oil field in Vung Tau, Vietnam that is able to utilize crude oil in the anaerobic condition is reported. The strain D107G3 was classified as a Gram-negative bacterium by using Gram staining method. Basing on scanning microscopy observation, the cell of a strain D107G3 had a curved rod shape. The 16S rRNA gene sequence analysis showed that the strain D107G3 was identified as Desulfovibrio vulgaris with 99.7% identity. The suitable conditions for its growth that was determined via estimating its H2S production was the modified Postgate B medium containing 1% (v/v) crude oil, 1% NaCl (w/v), pH 7 and 30°C incubation. In these conditions, the strain D107G3 can consume 11.4 % of crude oil total and oxidize heavy crude oil (≥ C45) for one month at anoxic condition. These obtained results not only contribute to the science but also continue to warn about the dangers of mesophilic sulfate reducing bacteria to the process of crude oil exploitation, use, and storage in Vung Tau, Vietnam. Trong bài báo này, chủng vi khuẩn khử sunphat (KSF) ưa ấm D107G3 phân lập từ giếng khoan dầu khí mỏ Bạch Hổ, Vũng Tàu, Việt Nam có khả năng sử dụng dầu thô trong điều kiện kị khí được công bố. Chủng D107G3 được xác định là vi khuẩn Gram âm nhờ phương pháp nhuộm Gram. Quan sát trên kính hiển vi điện tử quét cho thấy tế bào chủng D107G3 có hình que cong. Kết quả phân tích trình tự gen 16S rRNA đã xác định được chủng D107G3 thuộc loài Desulfovibrio vulgaris với độ tương đồng 99.7%. Thông qua đánh giá lượng H2S tạo thành đã khám phá được điều kiện thích hợp cho sinh trưởng của chủng D107G3: môi trường Postgate B cải tiến chứa 1% (v/v) dầu thô, 1 % NaCl (gL-1), pH 7 và nuôi cấy ở 30°C. Trong điều kiện đó, chủng D107G3 đã sử dụng được 11.4 % hàm lượng dầu tổng số, thành phần dầu bị phân huỷ là các n-parafin có mạch C≥45 sau 1 tháng nuôi cấy kỵ khí. Các kết quả này đóng góp về mặt khoa học và tiếp tục cảnh báo mối nguy hại của KSF ưa ấm đến việc khai thác, sử dụng và bảo quản dầu mỏ ở Vũng Tàu, Việt Nam.

scholarly journals Oil Field Souring Control by Nitrate-Reducing Sulfurospirillum spp. That Outcompete Sulfate-Reducing Bacteria for Organic Electron Donors

2007 ◽  
Vol 73 (8) ◽  
pp. 2644-2652 ◽  
Author(s):  
Casey Hubert ◽  
Gerrit Voordouw
Keyword(s):  
Community Analysis ◽  
Packed Bed ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Sulfate Concentration ◽  
Key Factors ◽  
Sulfate Reducing ◽  
Production Of Hydrogen ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

ABSTRACT Nitrate injection into oil reservoirs can prevent and remediate souring, the production of hydrogen sulfide by sulfate-reducing bacteria (SRB). Nitrate stimulates nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB) and heterotrophic nitrate-reducing bacteria (hNRB) that compete with SRB for degradable oil organics. Up-flow, packed-bed bioreactors inoculated with water produced from an oil field and injected with lactate, sulfate, and nitrate served as sources for isolating several NRB, including Sulfurospirillum and Thauera spp. The former coupled reduction of nitrate to nitrite and ammonia with oxidation of either lactate (hNRB activity) or sulfide (NR-SOB activity). Souring control in a bioreactor receiving 12.5 mM lactate and 6, 2, 0.75, or 0.013 mM sulfate always required injection of 10 mM nitrate, irrespective of the sulfate concentration. Community analysis revealed that at all but the lowest sulfate concentration (0.013 mM), significant SRB were present. At 0.013 mM sulfate, direct hNRB-mediated oxidation of lactate by nitrate appeared to be the dominant mechanism. The absence of significant SRB indicated that sulfur cycling does not occur at such low sulfate concentrations. The metabolically versatile Sulfurospirillum spp. were dominant when nitrate was present in the bioreactor. Analysis of cocultures of Desulfovibrio sp. strain Lac3, Lac6, or Lac15 and Sulfurospirillum sp. strain KW indicated its hNRB activity and ability to produce inhibitory concentrations of nitrite to be key factors for it to successfully outcompete oil field SRB.

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