Short-term temporal dynamics of yeast abundance on the tall fescue phylloplane

2008 ◽  
Vol 54 (4) ◽  
pp. 299-304 ◽  
Author(s):  
Shannon S. Nix ◽  
Leon L. Burpee ◽  
Kimberly L. Jackson ◽  
James W. Buck

Six replicate trials were conducted to determine the short-term temporal dynamics and the effects of foliar applications of nutrients on the phylloplane yeast community of tall fescue ( Festuca arundinacea Schreb.). In each trial, 2% sucrose + 0.5% yeast extract solution or sterile deionized water (control) was applied to the experiment plots. Twelve hours post-treatment (at 0600 hours), leaf samples were collected and yeast colony-forming units (cfu) were enumerated by dilution plating. This process was repeated at 1200, 1800, and 2400 hours in each trial. Significant differences were observed between the number of yeast cfu and the time at which the samples were collected. On average, the number of yeast cfu recovered was significantly less at 1800 hours and significantly greatest at 2400 hours when compared with all other sampling times. Averaged over all time intervals, we observed a trend of increased yeast abundance in turf treated with the nutrient solution compared with control treatments. In a separate investigation, atmospheric yeast abundance above the canopy of tall fescue was assessed in the morning (0900) and in the afternoon (1500) using a Thermo Andersen single stage viable particle sampler. In 5 of the 6 trials of this experiment, atmospheric yeast abundance was significantly greater in the morning than in the afternoon. Results suggest the following colonization model: phylloplane yeasts on tall fescue reproduce during the late evening and early morning, stabilize during the late morning and early afternoon through exchange of immigrants and emigrants, and decline during the late afternoon and (or) early evening.

1996 ◽  
Vol 271 (6) ◽  
pp. E1092-E1097 ◽  
Author(s):  
M. Bergo ◽  
G. Olivecrona ◽  
T. Olivecrona

The activity of lipoprotein lipase (LPL) in adipose tissue is modulated by changes in the nutritional status. We have measured LPL activity, mass, and mRNA levels in rat adipose tissue during normal feeding cycles, during short- and long-term fasting, and during refeeding after fasting. LPL activity displayed a diurnal rhythm. The activity was highest during the night and early morning, decreased to a minimum during the early afternoon, and then increased again. These changes corresponded to the feeding pattern. The increases and/or decreases resulted from changes in LPL synthetic rate compounded by posttranslational mechanisms. During short-term fasting, LPL specific activity decreased to < 30% of control. The specific activity was restored within 4 h by refeeding. On longer fasting, LPL mRNA decreased. This became significant from 36 h. On refeeding, it took 12 h to restore the mRNA levels, whereas tissue LPL activity and mass could not be fully restored by 36 h of refeeding. These data show that LPL activity during short-term fasting is regulated posttranscriptionally, which allows for quick upregulation after refeeding. On longer fasting, other mechanisms affecting LPL transcription and synthesis come into play, and upregulation after refeeding is slowed down.


2009 ◽  
Vol 55 (10) ◽  
pp. 1160-1165 ◽  
Author(s):  
Shannon Nix ◽  
Leon L. Burpee ◽  
James W. Buck

A growth-chamber experiment was conducted to determine how foliar disease or wounding affects the ability of 2 phylloplane yeasts ( Rhodotorula glutinis and Cryptococcus laurentii ) to colonize leaves of tall fescue ( Festuca arundinacea ). Yeasts were applied separately and together onto healthy leaves, leaves infected with Rhizoctonia solani (diseased), and mechanically bruised (wounded) leaves. In all 3 trials, the leaf disturbance treatment significantly affected the abundance of yeast on the phylloplane of tall fescue. Yeast abundance on the diseased or wounded leaves was significantly greater than on the nontreated, healthy leaves. In 2 of the 3 trials, the yeast species applied also had a significant affect on yeast abundance. Typically, R. glutinis was significantly more abundant than C. laurentii when applied individually, but not significantly greater than the total yeast colony-forming units of the co-inoculated treatment. When the 2 yeasts were co-inoculated onto the leaves, R. glutinis comprised 89.7%, 75.4%, and 67.6% of the recovered yeast colony-forming units on healthy, diseased, and wounded leaves, respectfully. Our data suggest that these 2 species of yeasts will differentially colonize compromised leaf tissue with disease or wounds favoring populations of R. glutinis over C. laurentii.


Author(s):  
J. Monk ◽  
E. Gerard ◽  
S. Young ◽  
K. Widdup ◽  
M. O'Callaghan

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation


Author(s):  
B.R. Watkin

AN Aberystwyth selection of tall fescue (Festuca arundinacea Schreb.), known as S170, was sown with certified New Zealand white clover (Trifolium repens) and re' clover (T. pratense) and compared under sheep grazing with other grass/clover pastures at the Grasslands Division Regional Station at Lincoln (Watkin, 1975) .


2021 ◽  
pp. 002071522199352
Author(s):  
Boris Heizmann ◽  
Nora Huth

This article addresses the extent to which economic downturns influence the perception of immigrants as an economic threat and through which channels this occurs. Our primary objective is an investigation of the specific mechanisms that connect economic conditions to the perception of immigrants as a threat. We therefore also contribute to theoretical discussions based on group threat and realistic group conflict theory by exposing the dominant source of competition relevant to these relationships. Furthermore, we investigate whether people react more sensitive to short-term economic dynamics within countries than to the long-term economic circumstances. Our database comprises all waves of the European Social Survey from 2002 to 2017. The macro-economic indicators we use include GDP per capita, unemployment, and national debt levels, covering the most salient economic dimensions. We furthermore control for the country’s migration situation and aggregate party positions toward cultural diversity. Our results show that the dynamic short-term developments of the economy and migration within countries are of greater relevance for perceived immigrant threat than the long-term situation. In contrast, the long-term political climate appears to be more important than short-term changes in the aggregate party positions. Further mediation analyses show that objective economic conditions influence anti-immigrant attitudes primarily through individual perceptions of the country’s economic performance and that unemployment rates are of primary importance.


Chemosphere ◽  
2021 ◽  
Vol 276 ◽  
pp. 130186
Author(s):  
ShaoFan Zuo ◽  
Shuai Hu ◽  
JinLiang Rao ◽  
Qin Dong ◽  
ZhaoLong Wang

2014 ◽  
Vol 10 (1) ◽  
pp. 39-47
Author(s):  
B.E. Bergstrom ◽  
J.H. Foreman ◽  
C.R. Foreman ◽  
A.M. Barger

Sodium bicarbonate and other alkalinising solutions (‘milkshakes’) have been given to horses surreptitiously before exercise to provide exogenous buffering effects. After an initial positive blood test, some accused horse trainers claim that their horses ‘naturally test high’, so some jurisdictions allow a secured quarantine in which the horse is tested multiple times. The objective of this experiment was to determine the intra- and inter-day variability of plasma total CO2 (tCO2) and other plasma strong ions in a group of sedentary horses housed similarly to a quarantine period. The hypothesis was that plasma tCO2 would not remain constant over a multi-day monitoring interval, but would vary measurably during that interval. Eight sedentary (unconditioned) horses were studied for 2 weeks. Horses were acclimated to a climate-controlled indoor environment and an alfalfa-only diet for a minimum of 10 days prior to sampling. Horses were sampled 3 times daily for 5 consecutive days at 7:00, 11:00 and 15:00 h. Blood samples were collected directly into 10 ml heparinised evacuated glass tubes by jugular venipuncture using a double-ended 0.91 mm needle. Samples were chilled until concentrations of plasma tCO2, Na+, K+, and Cl-, were determined within 1-3 h of sampling using an automated serum chemistry analyzer which was calibrated daily using commercial reagents obtained from the manufacturer as well as externally-obtained NIST-traceable calibrating solutions. Mean results documented mild variations in mean plasma tCO2 (range 28.9-31.6 mmol/l), but individual horses’ plasma tCO2 ranged over 4-7 units. Results showed that there was considerable intra- and inter-individual variability in plasma tCO2. Mean pooled tCO2 and measured strong ion difference (SIDm) differed by time-of-day, with both late morning and early afternoon values lower than early morning values (P<0.001). There was a strong positive linear relationship between plasma SIDm and tCO2 (r=0.75, P<0.001).


Sign in / Sign up

Export Citation Format

Share Document